ATE454463T1 - Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien - Google Patents

Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien

Info

Publication number
ATE454463T1
ATE454463T1 AT03711011T AT03711011T ATE454463T1 AT E454463 T1 ATE454463 T1 AT E454463T1 AT 03711011 T AT03711011 T AT 03711011T AT 03711011 T AT03711011 T AT 03711011T AT E454463 T1 ATE454463 T1 AT E454463T1
Authority
AT
Austria
Prior art keywords
closely related
extremozym
overexpression
pseudomonas
genes
Prior art date
Application number
AT03711011T
Other languages
English (en)
Inventor
Lawrence Chew
Henry Talbot
Stacey Lee
Original Assignee
Dow Global Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Inc filed Critical Dow Global Technologies Inc
Application granted granted Critical
Publication of ATE454463T1 publication Critical patent/ATE454463T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT03711011T 2002-02-13 2003-02-13 Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien ATE454463T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2002/004294 WO2003068948A1 (en) 2002-02-13 2002-02-13 Over-expression of extremozyme genes in pseudomonads and closely related bacteria
PCT/US2003/004288 WO2003068926A2 (en) 2002-02-13 2003-02-13 Over-expression of extremozyme genes in pseudomonads and closely related bacteria

Publications (1)

Publication Number Publication Date
ATE454463T1 true ATE454463T1 (de) 2010-01-15

Family

ID=27732085

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03711011T ATE454463T1 (de) 2002-02-13 2003-02-13 Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien

Country Status (14)

Country Link
US (1) US20050130160A1 (de)
EP (1) EP1476562B1 (de)
JP (1) JP2006501811A (de)
CN (1) CN100451102C (de)
AT (1) ATE454463T1 (de)
AU (2) AU2002306484A1 (de)
BR (1) BR0307630B1 (de)
CA (1) CA2475926A1 (de)
DE (1) DE60330841D1 (de)
DK (1) DK1476562T3 (de)
MX (1) MXPA04007886A (de)
PL (1) PL372107A1 (de)
RU (1) RU2004127236A (de)
WO (2) WO2003068948A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4472998B2 (ja) 2002-04-22 2010-06-02 ダウ グローバル テクノロジーズ インコーポレイティド 低コストのペプチド製造
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
JP5087741B2 (ja) * 2003-11-19 2012-12-05 フェネックス インコーポレイテッド 改良タンパク質発現系
CN1882605B (zh) 2003-11-21 2012-07-11 陶氏环球技术公司 使用Sec-系统分泌的改进的表达系统
EP2434016B1 (de) 2004-01-16 2018-11-07 Pfenex Inc. Expression von säugerproteinen in pseudomonas fluorescens
CN1926238A (zh) 2004-02-27 2007-03-07 陶氏化学公司 植物细胞中的高效肽生产
EP1774017B1 (de) 2004-07-26 2013-05-15 Pfenex Inc. Verfahren zur verbesserten proteinexpression durch strain-engineering
US8969033B2 (en) 2005-11-02 2015-03-03 Battelle Energy Alliance, Llc Alteration and modulation of protein activity by varying post-translational modification
US7727755B2 (en) * 2005-11-02 2010-06-01 Battelle Energy Alliance, Llc Enzyme and methodology for the treatment of a biomass
CA2677179C (en) 2007-01-31 2016-02-16 Dow Global Technologies Inc. Bacterial leader sequences for increased expression
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CN110577945A (zh) 2007-10-03 2019-12-17 维莱尼姆公司 木聚糖酶、编码它们的核酸以及其制备和应用方法
US8318481B2 (en) 2007-12-07 2012-11-27 Pfenex Inc. High copy number self-replicating plasmids in pseudomonas
US8492114B2 (en) 2008-01-25 2013-07-23 Battelle Energy Alliance, Llc Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
US9732330B2 (en) 2008-01-25 2017-08-15 Battelle Energy Alliance, Llc Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
JP2011523346A (ja) 2008-01-25 2011-08-11 バテル エナジー アライアンス，エルエルシー 熱耐性および酸耐性β‐キシロシダーゼ、遺伝子コード化、関連生物体、および方法
EP2235174A4 (de) 2008-01-31 2012-05-09 Battelle Energy Alliance Llc Thermophile und thermoacidophile biopolymerabbaugene, enzyme aus alicyclobacillus acidocaldarius sowie entsprechende organismen und verfahren
US8426185B2 (en) 2008-01-31 2013-04-23 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
US8497110B2 (en) 2008-01-31 2013-07-30 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
US8557557B2 (en) 2008-01-31 2013-10-15 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
EP2245040A4 (de) 2008-02-22 2013-06-05 Battelle Energy Alliance Llc Steuerung der transkription bei alicyclobacillus acidocaldarius und damit assoziierte gene, proteine und verfahren
EP2257566A4 (de) 2008-02-26 2012-05-30 Battelle Energy Alliance Llc Thermophile und thermoacidophile zuckertransportergene und enzyme aus alicyclobacillus acidocaldarius und damit in zusammenhang stehende organismen, verfahren
AU2009310370A1 (en) 2008-02-27 2010-05-06 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
CN102216451A (zh) 2008-02-28 2011-10-12 巴特勒能源同盟有限公司 来自酸热脂环酸杆菌的嗜热的与嗜热嗜酸的代谢基因和酶以及相关生物
CN101613681B (zh) * 2008-06-24 2013-07-10 中国科学院上海生命科学研究院 重组极端耐热α-淀粉酶的复性与纯化方法
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
WO2010132079A1 (en) * 2009-05-15 2010-11-18 University Of Florida Research Foundation, Inc. Increased expression of transhydrogenase genes and use thereof in ethanol production
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
US20110275135A1 (en) 2010-05-05 2011-11-10 Battelle Energy Alliance, Llc Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles
AR090321A1 (es) 2012-03-30 2014-11-05 Verenium Corp Genes que codifican celulasa
GB201308853D0 (en) 2013-03-12 2013-07-03 Verenium Corp Genes encoding xylanase
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
AR095173A1 (es) 2013-07-25 2015-09-30 Basf Enzymes Llc Fitasa
US11447714B2 (en) 2017-09-26 2022-09-20 Bunge Global Innovation, Llc Enzymatic removal of chlorophyll substrates from triacylglycerol-based oils
WO2020198212A1 (en) 2019-03-27 2020-10-01 Bunge Global Innovation, Llc Silica adsorbent treatment for removal of chlorophyll derivatives from triacylglycerol-based oils
PY2110525A (es) * 2020-02-10 2022-05-11 Novozymes As Polipéptidos con actividad de alfa amilasa y polinucleótidos que los codifican

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4753876A (en) 1984-03-21 1988-06-28 Monsanto Company Marker genes in pseudomonad bacteria
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US5028530A (en) 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
US6168945B1 (en) * 1988-03-23 2001-01-02 The Board Of Regents Of The University Of Oklahoma Genes encoding operon and promoter for branched chain keto acid dehydrogenase of pseudomonas putida and methods
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US5210025A (en) 1990-03-30 1993-05-11 University Of Alaska Prepressor protein gene for regulating expression of polypeptides and its use in the preparation of 2,2-dialkylglycine decarboxylase of Pseudomonas cepacia
US5356796A (en) 1990-03-30 1994-10-18 The University Of Alaska Repressor protein and operon for regulating expression of polypeptides and its use in the preparation of 2,2-dialkyglycine decarboxylase of Pseudomonas cepacia
US5670350A (en) 1990-08-20 1997-09-23 Novartis Finance Corporation Genomic DNA encoding a pseudomonas global transcriptional activation element and its use in activating gene expression
JPH074256B2 (ja) * 1990-10-31 1995-01-25 栗田工業株式会社 リパーゼの活性発現を調節する遺伝子、ベクターおよびリパーゼの生産方法
US5366883A (en) 1992-06-09 1994-11-22 Takara Shuzo Co., Ltd. α-amylase gene
US5578479A (en) * 1992-06-09 1996-11-26 The Johns Hopkins University Alpha-amylase from hyperthermophilic archaebacterium
EP0648843A1 (de) 1993-10-01 1995-04-19 Takara Shuzo Co. Ltd. Für ein hyperthermostabiles Alpha-amylase kodierendes DNA
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
GB2291058B (en) 1994-07-14 1998-12-23 Solvay Acid-stable and thermo-stable alpha-amylases derived from sufolobus species
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
WO1998045417A1 (en) 1997-04-09 1998-10-15 Michigan State University HYPERTHERMOSTABLE α-AMYLASE
US20040091968A1 (en) * 1997-08-13 2004-05-13 Short Jay M. Recombinant phytases and methods of making and using them
US6261842B1 (en) 1997-10-23 2001-07-17 Wisconsin Alumni Research Foundation Microorganism genomics, compositions and methods related thereto
US6090593A (en) 1998-05-13 2000-07-18 The United States Of America As Represented By The Secretary Of The Air Force Isolation of expressed genes in microorganisms
KR100312456B1 (ko) * 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
JP2000350585A (ja) * 1999-06-10 2000-12-19 Tokyo Gas Co Ltd 耐熱耐酸素性ヒドロゲナーゼ遺伝子
EP1144679A3 (de) * 1999-11-22 2002-02-06 Diversa Corporation Kapillar-anordnung und darauf basierte screeningverfahren
US20010047029A1 (en) 2000-04-26 2001-11-29 Handelsman Jo E. Triaryl cation antibiotics from environmental DNA
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens

Also Published As

Publication number Publication date
MXPA04007886A (es) 2004-10-15
EP1476562A2 (de) 2004-11-17
PL372107A1 (en) 2005-07-11
WO2003068926A2 (en) 2003-08-21
EP1476562A4 (de) 2005-12-07
DK1476562T3 (da) 2010-04-19
AU2003215197B2 (en) 2008-06-26
CA2475926A1 (en) 2003-08-21
BR0307630A (pt) 2005-01-11
RU2004127236A (ru) 2005-04-20
AU2003215197A1 (en) 2003-09-04
AU2002306484A1 (en) 2003-09-04
DE60330841D1 (de) 2010-02-25
CN100451102C (zh) 2009-01-14
EP1476562B1 (de) 2010-01-06
WO2003068926A3 (en) 2004-02-26
JP2006501811A (ja) 2006-01-19
CN1646694A (zh) 2005-07-27
US20050130160A1 (en) 2005-06-16
BR0307630B1 (pt) 2014-08-19
WO2003068948A1 (en) 2003-08-21

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