ATE446380T1 - Methode zur dns-sequenzierung - Google Patents

Methode zur dns-sequenzierung

Info

Publication number
ATE446380T1
ATE446380T1 AT00900717T AT00900717T ATE446380T1 AT E446380 T1 ATE446380 T1 AT E446380T1 AT 00900717 T AT00900717 T AT 00900717T AT 00900717 T AT00900717 T AT 00900717T AT E446380 T1 ATE446380 T1 AT E446380T1
Authority
AT
Austria
Prior art keywords
nucleic acid
target position
sample nucleic
dna sequencing
sequencing method
Prior art date
Application number
AT00900717T
Other languages
English (en)
Inventor
Mostafa Ronaghi
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE446380T1 publication Critical patent/ATE446380T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00900717T 1999-01-22 2000-01-20 Methode zur dns-sequenzierung ATE446380T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9901475.5A GB9901475D0 (en) 1999-01-22 1999-01-22 A method of DNA sequencing
PCT/GB2000/000146 WO2000043540A1 (en) 1999-01-22 2000-01-20 A method of dna sequencing

Publications (1)

Publication Number Publication Date
ATE446380T1 true ATE446380T1 (de) 2009-11-15

Family

ID=10846383

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00900717T ATE446380T1 (de) 1999-01-22 2000-01-20 Methode zur dns-sequenzierung

Country Status (8)

Country Link
US (1) US6828100B1 (de)
EP (1) EP1147217B1 (de)
JP (1) JP4951170B2 (de)
AT (1) ATE446380T1 (de)
AU (1) AU3064200A (de)
DE (1) DE60043182D1 (de)
GB (1) GB9901475D0 (de)
WO (1) WO2000043540A1 (de)

Families Citing this family (401)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20030108867A1 (en) * 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US20050244870A1 (en) * 1999-04-20 2005-11-03 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
GB0021977D0 (en) * 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0022069D0 (en) * 2000-09-08 2000-10-25 Pyrosequencing Ab Method
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
GB2390055B (en) 2002-03-22 2005-09-07 Cypher Science Internat Ltd A marking apparatus
US7595883B1 (en) * 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
DE60324819D1 (de) 2002-09-17 2009-01-02 Aisin Cosmos R & D Co Ltd Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
AU2003265185A1 (en) * 2002-09-27 2004-04-19 Biotage Ab New sequencing method for sequencing rna molecules
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
CN1791682B (zh) * 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
ATE418621T1 (de) 2003-04-24 2009-01-15 Afshin Ahmadian Verfahren zur detektion allelspezifischer mutationen
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
US20070111213A1 (en) 2003-10-28 2007-05-17 Lu Wang Primers, methods and kits for amplifying or detecting human leukocyte antigen alleles
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7759057B2 (en) * 2004-02-04 2010-07-20 Ut-Battelle, Llc Method for analyzing microbial communities
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP1766090B1 (de) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Verfahren zur nukleinsäureimmobilisierung
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
AU2005254984B2 (en) * 2004-06-10 2008-10-16 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
US7361465B2 (en) * 2004-09-07 2008-04-22 Applera Corporation Methods and compositions for tailing and amplifying RNA
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CA2594483C (en) 2005-01-28 2014-08-26 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
WO2007002890A2 (en) * 2005-06-28 2007-01-04 Agencourt Personal Genomics Corporation Methods of producing and sequencing modified polynucleotides
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
JP4353958B2 (ja) * 2005-09-15 2009-10-28 株式会社日立製作所 Dna計測装置、及びdna計測方法
EP1951900B1 (de) 2005-10-07 2016-06-15 Callida Genomics, Inc. Selbstzusammengesetzte einzelmolekül-arrays und verwendungen davon
EP2578702A1 (de) 2006-02-16 2013-04-10 454 Life Sciences Corporation System Und Verfahren Zur Korrektur Von Primerextensionsfehlern Bei Nukleinsäuresequenzdaten
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8613889B2 (en) * 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US8637317B2 (en) * 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US7816121B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US7727723B2 (en) 2006-04-18 2010-06-01 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
WO2007123908A2 (en) * 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7763471B2 (en) * 2006-04-18 2010-07-27 Advanced Liquid Logic, Inc. Method of electrowetting droplet operations for protein crystallization
US8470606B2 (en) * 2006-04-18 2013-06-25 Duke University Manipulation of beads in droplets and methods for splitting droplets
US7939021B2 (en) 2007-05-09 2011-05-10 Advanced Liquid Logic, Inc. Droplet actuator analyzer with cartridge
US7822510B2 (en) 2006-05-09 2010-10-26 Advanced Liquid Logic, Inc. Systems, methods, and products for graphically illustrating and controlling a droplet actuator
US8041463B2 (en) * 2006-05-09 2011-10-18 Advanced Liquid Logic, Inc. Modular droplet actuator drive
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (de) 2006-12-14 2014-11-19 Life Technologies Corporation Verfahren zur Sequenzierung einer Nukleinsäure mittels grosser FET-Arrays, geeignet zur Messung in einem begrenzten pH-Bereich
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US8551704B2 (en) * 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
CN101720359A (zh) * 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
EP2173898A2 (de) 2007-06-28 2010-04-14 454 Life Sciences Corporation System und verfahren zur adaptiven reagenskontrolle bei der nukleinsäuresequenzierung
WO2009021233A2 (en) 2007-08-09 2009-02-12 Advanced Liquid Logic, Inc. Pcb droplet actuator fabrication
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
WO2009061840A1 (en) 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
WO2009073629A2 (en) 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN103290106B (zh) 2007-12-05 2015-07-29 考利达基因组股份有限公司 测序反应中碱基的有效确定
WO2009097368A2 (en) * 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US8206905B2 (en) * 2008-04-26 2012-06-26 Valeri Golovlev Enzymatic time-resolved luminescent assay for nucleic acids quantitation
WO2010008480A2 (en) 2008-06-25 2010-01-21 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
WO2010003153A2 (en) * 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
US20100261189A1 (en) * 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2394165A4 (de) * 2009-02-03 2013-12-11 Complete Genomics Inc Zuordnung von oligomersequenzen
US8738296B2 (en) * 2009-02-03 2014-05-27 Complete Genomics, Inc. Indexing a reference sequence for oligomer sequence mapping
US8615365B2 (en) * 2009-02-03 2013-12-24 Complete Genomics, Inc. Oligomer sequences mapping
US20110020806A1 (en) * 2009-03-31 2011-01-27 The Regents Of The University Of California Rapid DNA Sequencing by Peroxidative Reaction
EP2511843B1 (de) * 2009-04-29 2016-12-21 Complete Genomics, Inc. Verfahren und System zum Aufrufen von Variationen in einer Polynukleotidprobensequenz in Bezug auf eine Referenzpolynukleotidsequenz
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
EP2425240A4 (de) 2009-04-30 2012-12-12 Good Start Genetics Inc Verfahren und zusammensetzung zur bewertung genetischer marker
JP5618497B2 (ja) * 2009-05-15 2014-11-05 株式会社日立ハイテクノロジーズ 核酸分析方法及び核酸分析装置
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CN102459592B (zh) 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
US8609339B2 (en) 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
BR112012009023A2 (pt) 2009-10-22 2019-09-24 Biotools Biotechnologial & Medical Laboratories S A método pra detecção. diferenciação e identificação de eubactérias específica de táxons em uma amostra biológica por meio de técnicas de análise de sequenciamento e kit
US8574832B2 (en) 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
SG184466A1 (en) 2010-04-06 2012-11-29 Massachusetts Inst Technology Gene-expression profiling with reduced numbers of transcript measurements
US9125931B2 (en) 2010-04-06 2015-09-08 Massachusetts Institute Of Technology Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
WO2011135041A1 (en) 2010-04-30 2011-11-03 Roche Diagnostics Gmbh System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US20120035062A1 (en) 2010-06-11 2012-02-09 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
JP2013533482A (ja) 2010-06-30 2013-08-22 ライフ テクノロジーズ コーポレーション イオン感応性電荷蓄積回路および方法
US20120001646A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Methods and apparatus for testing isfet arrays
US8432149B2 (en) 2010-06-30 2013-04-30 Life Technologies Corporation Array column integrator
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
CN103168341B (zh) 2010-07-03 2016-10-05 生命科技公司 具有轻度掺杂的排出装置的化学敏感的传感器
EP2407242A1 (de) 2010-07-13 2012-01-18 Dublin City University Direkte Klonanalyse und Auswahltechnologie
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US9880089B2 (en) 2010-08-31 2018-01-30 Complete Genomics, Inc. High-density devices with synchronous tracks for quad-cell based alignment correction
US9671344B2 (en) 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
EP2617061B1 (de) 2010-09-15 2021-06-30 Life Technologies Corporation Verfahren und vorrichtung zur analytmessung
CN103299182A (zh) 2010-09-24 2013-09-11 生命科技公司 匹配的晶体管对电路
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
EP2633470B1 (de) 2010-10-27 2016-10-26 Life Technologies Corporation Prädiktives modell zur verwendung für sequenzierung nach synthese
WO2012082464A2 (en) 2010-12-14 2012-06-21 Life Technologies Corporation Systems and methods for run-time sequencing run quality monitoring
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2658999B1 (de) 2010-12-30 2019-03-13 Life Technologies Corporation Modelle zur analyse von daten aus sequenzierung-mit-synthese-operationen
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
US20120258871A1 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
WO2012138921A1 (en) 2011-04-08 2012-10-11 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
EP2768983A4 (de) 2011-10-17 2015-06-03 Good Start Genetics Inc Analyseverfahren
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
WO2013166517A1 (en) 2012-05-04 2013-11-07 Complete Genomics, Inc. Methods for determining absolute genome-wide copy number variations of complex tumors
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
US9884893B2 (en) 2012-05-21 2018-02-06 Distributed Bio, Inc. Epitope focusing by variable effective antigen surface concentration
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
WO2013184905A1 (en) 2012-06-06 2013-12-12 Myriad Genetics, Inc. Hereditary cancer genes
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
EP3901280B1 (de) 2012-10-17 2025-03-12 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
WO2014074611A1 (en) 2012-11-07 2014-05-15 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
EP2971159B1 (de) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Verfahren zur analyse von nukleinsäuren
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
CN105283758B (zh) 2013-03-15 2018-06-05 生命科技公司 具有一致传感器表面区域的化学传感器
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
WO2014149780A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
EP2972281B1 (de) 2013-03-15 2023-07-26 Life Technologies Corporation Chemische vorrichtung mit dünnem leitenden element
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
WO2014197377A2 (en) 2013-06-03 2014-12-11 Good Start Genetics, Inc. Methods and systems for storing sequence read data
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
CN105074011B (zh) 2013-06-13 2020-10-02 阿瑞奥萨诊断公司 用于非入侵性性染色体非整倍性确定的统计分析
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
EP2840148B1 (de) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Verfahren für nukleinsäureamplifikation
CN105637099B (zh) 2013-08-23 2020-05-19 深圳华大智造科技有限公司 使用短读段的长片段从头组装
EP3049539B1 (de) 2013-09-25 2018-09-05 Bio-Id Diagnostic Inc. Verfahren für den nachweis von nukleinsäurefragmenten
JP6532456B2 (ja) 2013-10-04 2019-06-19 ライフ テクノロジーズ コーポレーション 終止化学を用いる配列決定における整相効果(phasing effects)をモデル化するための方法及びシステム
WO2015058120A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
JP2017500004A (ja) 2013-10-18 2017-01-05 セブン ブリッジズ ジェノミクス インコーポレイテッド 遺伝子試料について遺伝子型解析するための方法およびシステム
US10832797B2 (en) 2013-10-18 2020-11-10 Seven Bridges Genomics Inc. Method and system for quantifying sequence alignment
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US10053736B2 (en) 2013-10-18 2018-08-21 Seven Bridges Genomics Inc. Methods and systems for identifying disease-induced mutations
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US9092402B2 (en) 2013-10-21 2015-07-28 Seven Bridges Genomics Inc. Systems and methods for using paired-end data in directed acyclic structure
US10867693B2 (en) 2014-01-10 2020-12-15 Seven Bridges Genomics Inc. Systems and methods for use of known alleles in read mapping
US9817944B2 (en) 2014-02-11 2017-11-14 Seven Bridges Genomics Inc. Systems and methods for analyzing sequence data
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US10450562B2 (en) 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
US10429399B2 (en) 2014-09-24 2019-10-01 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
US9558321B2 (en) 2014-10-14 2017-01-31 Seven Bridges Genomics Inc. Systems and methods for smart tools in sequence pipelines
JP2017530720A (ja) 2014-10-17 2017-10-19 グッド スタート ジェネティクス, インコーポレイテッド 着床前遺伝子スクリーニングおよび異数性検出
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
CN107407656B (zh) 2014-12-18 2020-04-07 生命科技公司 使用大规模 fet 阵列测量分析物的方法和装置
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
TWI756167B (zh) 2014-12-18 2022-03-01 美商生命技術公司 積體電路裝置、感測器裝置及積體電路
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
JP6929778B2 (ja) 2015-01-15 2021-09-01 グッド スタート ジェネティクス, インコーポレイテッド 着床前遺伝子スクリーニングにおける一塩基多型を用いた品質管理方法
WO2016133764A1 (en) 2015-02-17 2016-08-25 Complete Genomics, Inc. Dna sequencing using controlled strand displacement
US10192026B2 (en) 2015-03-05 2019-01-29 Seven Bridges Genomics Inc. Systems and methods for genomic pattern analysis
WO2016156529A1 (en) * 2015-03-31 2016-10-06 Qiagen Gmbh Efficiency improving ligation methods
EP4321627A3 (de) 2015-04-10 2024-04-17 10x Genomics Sweden AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
EP4220645A3 (de) 2015-05-14 2023-11-08 Life Technologies Corporation Barcodesequenzen sowie zugehörige systeme und verfahren
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
US10563266B2 (en) 2015-07-14 2020-02-18 Personal Genome Diagnostics Inc. Neoantigen treatment prioritization using multivariate analysis based on: HLA genotype, self-similarity, similarity to known antigens, antigen expression levels and mutant allele frequency
CN108350500A (zh) 2015-07-29 2018-07-31 普罗格尼迪公司 用于检测染色体异常的核酸和方法
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
US12603152B2 (en) 2015-10-10 2026-04-14 Guardant Health, Inc. Methods and applications of gene fusion detection in cell-free DNA analysis
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
EP3377647B1 (de) 2015-11-16 2020-03-11 Progenity, Inc. Nukleinsäuren und verfahren zum nachweis des methylierungsstatus
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
US20170199960A1 (en) 2016-01-07 2017-07-13 Seven Bridges Genomics Inc. Systems and methods for adaptive local alignment for graph genomes
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
WO2017168331A1 (en) 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex fragment sequencing
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
EP3478702A4 (de) 2016-06-27 2020-03-18 Dana-Farber Cancer Institute, Inc. Verfahren zur messung der rna-translationsraten
US10600499B2 (en) 2016-07-13 2020-03-24 Seven Bridges Genomics Inc. Systems and methods for reconciling variants in sequence data relative to reference sequence data
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
WO2018064311A2 (en) 2016-09-28 2018-04-05 Life Technologies Corporation Methods and systems for reducing phasing errors when sequencing nucleic acids using termination chemistry
BR112019008939B1 (pt) 2016-11-03 2023-11-07 Mgi Tech Co., Ltd Biossensor, método, e, método para sequenciamento de dna
CA3042434A1 (en) 2016-11-15 2018-05-24 Personal Genome Diagnostics Inc. Non-unique barcodes in a genotyping assay
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
FI4112741T3 (fi) 2017-01-04 2025-05-15 Mgi Tech Co Ltd Vaiheittainen sekvensointi ei-leimatuilla, reversiibeleillä terminaattoreilla tai luonnollisilla nukleotideilla
KR102535751B1 (ko) 2017-03-20 2023-05-22 엠쥐아이 테크 컴퍼니 엘티디. 생물학적 또는 화학적 분석을 위한 바이오센서 및 그 제조 방법
WO2019060177A1 (en) 2017-09-19 2019-03-28 Complete Genomics, Inc. MANUFACTURE OF SEQUENCING FLOW CELLS AT THE WAFER
EP4589059A3 (de) 2017-12-01 2025-12-10 Personal Genome Diagnostics, Inc. Verfahren zur detektion von mikrosatelliteninstabilität
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20200075123A1 (en) 2018-08-31 2020-03-05 Guardant Health, Inc. Genetic variant detection based on merged and unmerged reads
CN120082639A (zh) 2018-11-09 2025-06-03 深圳华大智造科技股份有限公司 使用未标记的核苷酸进行大规模平行测序
TWI853851B (zh) 2018-11-15 2024-09-01 中國商深圳華大智造科技有限公司 微流體裝置與其製備方法
WO2020108588A1 (en) 2018-12-01 2020-06-04 Mgi Tech Co., Ltd. Methods and structures to improve light collection efficiency in biosensors
EP3894585A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Erzeugung von erfassungssonden zur räumlichen analyse
EP4538389A3 (de) 2019-01-03 2025-07-30 Ncan Genomics, Inc. Verknüpfte zielerfassung
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11346755B2 (en) 2019-01-10 2022-05-31 Travera, Inc. Calibration of a functional biomarker instrument
CA3126147A1 (en) 2019-01-10 2020-07-16 Travera LLC Machine learning in functional cancer assays
US12362038B2 (en) 2019-01-10 2025-07-15 Travera, Inc. Identifying cancer therapies
US12560590B2 (en) 2019-01-23 2026-02-24 Massachusetts Institute Of Technology Rapid analysis of live cells
WO2020180813A1 (en) 2019-03-06 2020-09-10 Qiagen Sciences, Llc Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
EP4752521A3 (de) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zur verwendung davon
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
US12663345B2 (en) 2020-01-13 2026-06-23 Illumina, Inc. Devices for generating monodisperse droplets from a bulk liquid
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021185320A1 (en) 2020-03-18 2021-09-23 Mgi Tech Co., Ltd. Restoring phase in massively parallel sequencing
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4153775B1 (de) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4600376A3 (de) 2020-06-02 2025-10-22 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4164796A4 (de) 2020-06-10 2024-03-06 10x Genomics, Inc. Flüssigkeitsabgabeverfahren
EP4450639B1 (de) 2020-06-25 2025-10-15 10X Genomics, Inc. Räumliche analyse der dna-methylierung
CN111635932B (zh) * 2020-06-30 2022-07-08 北京启衡星生物科技有限公司 一种核酸聚合酶活性检测方法的应用及试剂盒
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
US20220042109A1 (en) 2020-08-06 2022-02-10 Agendia NV Methods of assessing breast cancer using circulating hormone receptor transcripts
WO2022029495A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of scoring risk and residual disease from passenger mutations
US20220042108A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of assessing breast cancer
US20220042106A1 (en) 2020-08-06 2022-02-10 Agendia NV Systems and methods of using cell-free nucleic acids to tailor cancer treatment
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
KR102924185B1 (ko) 2020-10-13 2026-02-06 바이오-라드 유럽 게엠베하 식품 품질을 특성화하고 식품 안전을 개선할 때 적용되는 표적 검출을 위한 시스템 및 방법
EP4228815A4 (de) 2020-10-19 2024-10-16 Bio-Rad Laboratories, Inc. System und verfahren zur schnellen multiplexierten probenverarbeitung mit anwendungen für nukleinsäureamplifikationstests
ES3063619T3 (en) 2020-11-03 2026-04-17 Illumina Inc Systems and methods for making sequencing libraries
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
EP4305190A4 (de) 2021-03-11 2025-03-26 Iggenix, Inc. Verfahren und systeme zur vorhersage einer allergischen reaktion
EP4524261B1 (de) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
US12176070B2 (en) * 2021-04-19 2024-12-24 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
WO2022245827A1 (en) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Pre-templated instant partitions for screening
WO2022245868A1 (en) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Perturbed genomic expression in pretemplated instant partitions
WO2022245825A1 (en) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Multi-omic analysis of extracellular vesicles in monodisperse droplets
WO2022251445A1 (en) 2021-05-28 2022-12-01 Fluent Biosciences Inc. Spatial transcriptomics in pips
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4363611A1 (de) 2021-06-30 2024-05-08 Dana-Farber Cancer Institute, Inc. Zusammensetzungen und verfahren zur anreicherung von nukleinsäuren mit lichtvermittelter vernetzung
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4426475A4 (de) 2021-11-03 2025-12-24 Fluent Biosciences Inc Vorrichtungen zur verbesserung der probenvorbereitung und -verarbeitung
EP4419707A1 (de) 2021-11-10 2024-08-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position eines analyten in einer biologischen probe
EP4305195A2 (de) 2021-12-01 2024-01-17 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
US11639499B1 (en) 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
EP4685239A1 (de) 2022-11-09 2026-01-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
WO2024173265A1 (en) 2023-02-13 2024-08-22 Guardant Health, Inc. Characterization of whole genome duplication in a genomic cohort of over 14000 cell free dna samples
CN121100381A (zh) 2023-04-17 2025-12-09 私人基因诊断公司 通过全基因组测序的综合分析进行的癌症检测
EP4739790A1 (de) 2023-07-06 2026-05-13 Pacific Biosciences of California, Inc. Verfahren und zusammensetzungen zur stabilisierung von konkateren
WO2025019319A1 (en) 2023-07-14 2025-01-23 The Broad Institute, Inc. Preparation of molecular libraries by linked adapters
WO2025019313A1 (en) 2023-07-14 2025-01-23 The Broad Institute, Inc. Method for assembling gene libraries from a pool of oligonucleotides
US12157920B1 (en) 2023-11-15 2024-12-03 Droplet Biosciences, Inc. Methods for diagnosing and assessing disease using drain fluid
WO2025178954A1 (en) 2024-02-23 2025-08-28 Illumina, Inc. Methods for sequencing and counting nucleic acids
US20250279155A1 (en) 2024-03-04 2025-09-04 Omniseq, Inc. Consensus-based classification technique to determine genetically inffered ancestry from comprehensive genomic profiling of tumor dna
WO2025250836A1 (en) 2024-05-29 2025-12-04 Omniseq, Inc. Ai-based multi-omics data processing for detection of genomic instability
WO2025254714A1 (en) 2024-06-03 2025-12-11 Illumina, Inc. System and method for assembling a polynucleotide sequence
WO2026059922A1 (en) 2024-09-10 2026-03-19 Illumina, Inc. Molecular deduplication analysis methods
WO2026064275A1 (en) 2024-09-20 2026-03-26 Somalogic, Inc. Methods for detection of proteins and nucleic acids
WO2026102281A1 (en) 2024-11-08 2026-05-15 Illumina, Inc. On-flow cell library preparation for single-cell rna analysis

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646019A (en) 1989-10-24 1997-07-08 Stratagene Method for producing primed nucleic acid templates
US5449603A (en) 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
US5605824A (en) 1989-10-24 1997-02-25 Stratagene Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein
GB9210168D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Method of sequencing dna
US5547843A (en) 1992-07-17 1996-08-20 Associated Universities, Inc. Method for promoting specific alignment of short oligonucleotides on nucleic acids
US6075905A (en) 1996-07-17 2000-06-13 Sarnoff Corporation Method and apparatus for mosaic image construction
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) * 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
AU7813698A (en) 1997-06-06 1998-12-21 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
US6335162B1 (en) * 1998-03-13 2002-01-01 Promega Corporation Nucleic acid detection

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US6828100B1 (en) 2004-12-07
JP4951170B2 (ja) 2012-06-13
DE60043182D1 (de) 2009-12-03
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GB9901475D0 (en) 1999-03-17
EP1147217A1 (de) 2001-10-24

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