ATE452992T1 - Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation - Google Patents
Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikationInfo
- Publication number
- ATE452992T1 ATE452992T1 AT05712856T AT05712856T ATE452992T1 AT E452992 T1 ATE452992 T1 AT E452992T1 AT 05712856 T AT05712856 T AT 05712856T AT 05712856 T AT05712856 T AT 05712856T AT E452992 T1 ATE452992 T1 AT E452992T1
- Authority
- AT
- Austria
- Prior art keywords
- dutp
- nucleic acid
- acid amplification
- formation during
- based compositions
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title abstract 5
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 230000015572 biosynthetic process Effects 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Exposure Of Semiconductors, Excluding Electron Or Ion Beam Exposure (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54199904P | 2004-02-04 | 2004-02-04 | |
| PCT/US2005/003567 WO2005076925A2 (en) | 2004-02-04 | 2005-02-04 | Dutp based compositions for reducing primer-aggregate formation during nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE452992T1 true ATE452992T1 (de) | 2010-01-15 |
Family
ID=34860246
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05712856T ATE452992T1 (de) | 2004-02-04 | 2005-02-04 | Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation |
| AT05712812T ATE440948T1 (de) | 2004-02-04 | 2005-02-04 | Durch gefrierschutzprotein verbesserte nukleinsäureamplifikation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05712812T ATE440948T1 (de) | 2004-02-04 | 2005-02-04 | Durch gefrierschutzprotein verbesserte nukleinsäureamplifikation |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US7932059B2 (de) |
| EP (2) | EP1725680B1 (de) |
| JP (2) | JP4718493B2 (de) |
| AT (2) | ATE452992T1 (de) |
| DE (2) | DE602005016221D1 (de) |
| WO (2) | WO2005076925A2 (de) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008206491A (ja) * | 2007-02-28 | 2008-09-11 | Toyobo Co Ltd | ｐ−ヒドロキシ安息香酸水酸化酵素の安定化方法 |
| JP5608998B2 (ja) * | 2009-03-31 | 2014-10-22 | 東洋紡株式会社 | 保存安定性に優れた核酸増幅検出試薬キット |
| JP6060447B2 (ja) | 2011-04-08 | 2017-01-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 非特異的活性が低下したＳｓｏ７ポリメラーゼコンジュゲート |
| CN103703141B (zh) | 2011-04-08 | 2016-08-17 | 伯乐实验室公司 | 具有降低的非特异性活性的pcr反应混合物 |
| FI3461807T3 (fi) | 2011-06-08 | 2023-09-07 | Life Technologies Corp | Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten |
| US9567628B2 (en) | 2011-06-08 | 2017-02-14 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| EP3539944A1 (de) | 2013-10-25 | 2019-09-18 | Life Technologies Corporation | Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon |
| EP3102699B1 (de) | 2014-02-07 | 2018-10-31 | Qiagen Sciences, LLC | Pcr-primer |
| CN104389026A (zh) * | 2014-10-30 | 2015-03-04 | 北京诺禾致源生物信息科技有限公司 | 单细胞转录组测序文库的构建方法及其应用 |
| US10822645B1 (en) | 2014-12-23 | 2020-11-03 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| US10858694B2 (en) | 2014-12-23 | 2020-12-08 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| US10968478B2 (en) | 2014-12-23 | 2021-04-06 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| CN108645676B (zh) * | 2018-05-25 | 2020-07-14 | 吉林建筑大学 | 基于超声波技术的季冻区路基土材料损伤检测与评价方法 |
| CN109609495B (zh) * | 2018-12-31 | 2023-05-23 | 苏州近岸蛋白质科技股份有限公司 | 一种高纯度单链dna制备方法及其应用 |
| CN112608984B (zh) * | 2020-12-17 | 2022-12-06 | 厦门大学 | 一种用于核酸扩增的预装pcr反应试剂及其制备方法 |
| EP4581154A1 (de) | 2022-08-31 | 2025-07-09 | QIAGEN GmbH | Gefriergetrocknete mischung zur amplifikation von nukleinsäuren |
| EP4581153A1 (de) * | 2022-09-02 | 2025-07-09 | F. Hoffmann-La Roche AG | Verbesserungen der zielanreicherungsleistung der nächsten generation |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3223104A1 (de) * | 1982-06-21 | 1983-12-22 | Hoechst Ag, 6230 Frankfurt | Photopolymerisierbares gemisch und damit hergestelltes photopolymerisierbares kopiermaterial |
| US4737462A (en) * | 1982-10-19 | 1988-04-12 | Cetus Corporation | Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of interferon-β |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4806343A (en) * | 1986-03-13 | 1989-02-21 | University Of Southwestern Louisiana | Cryogenic protectant for proteins |
| US5418149A (en) * | 1990-07-24 | 1995-05-23 | Hoffmann-La Roche Inc. | Reduction of non-specific amplification glycosylase using DUTP and DNA uracil |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5536648A (en) * | 1988-12-09 | 1996-07-16 | Amrad Corporation Limited | Amplified DNA assay using a double stranded DNA binding protein |
| US5605824A (en) * | 1989-10-24 | 1997-02-25 | Stratagene | Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| AU659795B2 (en) * | 1990-01-17 | 1995-06-01 | Regents Of The University Of California, The | Composition to improve survival of biological materials |
| US5932697A (en) * | 1990-09-25 | 1999-08-03 | Virginia Tech Intellectual Properties, Inc. | Synthetic antifreeze peptide |
| US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| CA2122203C (en) * | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
| US5731171A (en) * | 1993-07-23 | 1998-03-24 | Arch Development Corp. | Sequence independent amplification of DNA |
| US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| US6307020B1 (en) * | 1996-01-31 | 2001-10-23 | Hsc Research And Development Ltd. Partnership | Intracellular antifreeze polypeptides and nucleic acids |
| US6413718B1 (en) * | 1996-05-01 | 2002-07-02 | Visible Genetics Inc. | Method for sequencing of nucleic acid polymers |
| ATE317899T1 (de) * | 1996-06-03 | 2006-03-15 | Gefrierschutzproteine aus choristoneura sp., gene und verfahren zu ihrer verwendung | |
| WO1998054309A1 (fr) * | 1997-05-26 | 1998-12-03 | Shionogi & Co., Ltd. | DIAGNOSTIC GENETIQUE DE MALADIES DANS LESQUELLES INTERVIENT LE PROMOTEUR DU TNF-$g(a) |
| DE19736062A1 (de) * | 1997-08-20 | 1999-02-25 | Boehringer Mannheim Gmbh | Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen |
| AU752833B2 (en) * | 1998-04-01 | 2002-10-03 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Anthrax lethal factor is a MAPK kinase protease |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| EP1412495A2 (de) * | 2001-05-10 | 2004-04-28 | Novozymes A/S | Verfahren zur herstellung rekombinierter polynukleotide |
| US6783940B2 (en) * | 2001-10-31 | 2004-08-31 | Applera Corporation | Method of reducing non-specific amplification in PCR |
| GB0126887D0 (en) | 2001-11-08 | 2002-01-02 | Univ London | Method for producing and identifying soluble protein domains |
| JP2003250572A (ja) * | 2002-03-04 | 2003-09-09 | National Institute Of Advanced Industrial & Technology | ワカサギ由来抗凍結タンパク質、その回収方法、及びその用途 |
| CA2498353C (en) * | 2002-09-09 | 2014-12-02 | Genesis Research And Development Corporation Limited | Antifreeze proteins isolated from forage grasses and methods for their use |
-
2005
- 2005-02-04 AT AT05712856T patent/ATE452992T1/de not_active IP Right Cessation
- 2005-02-04 EP EP05712856A patent/EP1725680B1/de not_active Ceased
- 2005-02-04 US US10/588,689 patent/US7932059B2/en not_active Expired - Fee Related
- 2005-02-04 WO PCT/US2005/003567 patent/WO2005076925A2/en not_active Ceased
- 2005-02-04 WO PCT/US2005/003502 patent/WO2005076908A2/en not_active Ceased
- 2005-02-04 DE DE602005016221T patent/DE602005016221D1/de not_active Expired - Lifetime
- 2005-02-04 AT AT05712812T patent/ATE440948T1/de not_active IP Right Cessation
- 2005-02-04 JP JP2006552262A patent/JP4718493B2/ja not_active Expired - Lifetime
- 2005-02-04 US US10/588,587 patent/US20100035238A1/en not_active Abandoned
- 2005-02-04 EP EP05712812A patent/EP1718743B1/de not_active Expired - Lifetime
- 2005-02-04 JP JP2006552251A patent/JP2007520236A/ja active Pending
- 2005-02-04 DE DE602005018460T patent/DE602005018460D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7932059B2 (en) | 2011-04-26 |
| EP1718743A2 (de) | 2006-11-08 |
| EP1718743A4 (de) | 2007-03-28 |
| EP1718743B1 (de) | 2009-08-26 |
| WO2005076908A3 (en) | 2006-01-19 |
| JP2007520236A (ja) | 2007-07-26 |
| EP1725680B1 (de) | 2009-12-23 |
| WO2005076925A2 (en) | 2005-08-25 |
| US20100035238A1 (en) | 2010-02-11 |
| EP1725680A4 (de) | 2008-05-21 |
| EP1725680A2 (de) | 2006-11-29 |
| US20070264633A1 (en) | 2007-11-15 |
| WO2005076925A3 (en) | 2005-10-13 |
| ATE440948T1 (de) | 2009-09-15 |
| JP4718493B2 (ja) | 2011-07-06 |
| DE602005018460D1 (de) | 2010-02-04 |
| DE602005016221D1 (de) | 2009-10-08 |
| JP2007520237A (ja) | 2007-07-26 |
| WO2005076908A2 (en) | 2005-08-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |