ATE481507T1 - Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv - Google Patents
Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcvInfo
- Publication number
- ATE481507T1 ATE481507T1 AT05712046T AT05712046T ATE481507T1 AT E481507 T1 ATE481507 T1 AT E481507T1 AT 05712046 T AT05712046 T AT 05712046T AT 05712046 T AT05712046 T AT 05712046T AT E481507 T1 ATE481507 T1 AT E481507T1
- Authority
- AT
- Austria
- Prior art keywords
- hcv
- primer
- detection
- amplification
- probe design
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 5
- 238000001514 detection method Methods 0.000 title 1
- 239000000872 buffer Substances 0.000 abstract 4
- 239000002773 nucleotide Substances 0.000 abstract 3
- -1 nucleotide triphosphates Chemical class 0.000 abstract 3
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 239000001226 triphosphate Substances 0.000 abstract 2
- 235000011178 triphosphate Nutrition 0.000 abstract 2
- 239000001177 diphosphate Substances 0.000 abstract 1
- 235000011180 diphosphates Nutrition 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53881404P | 2004-01-23 | 2004-01-23 | |
| US53881604P | 2004-01-23 | 2004-01-23 | |
| US53881504P | 2004-01-23 | 2004-01-23 | |
| PCT/US2005/002415 WO2005071117A2 (en) | 2004-01-23 | 2005-01-21 | Primer and probe design for efficient amplification and detection of hcv 3′ non-translating region |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE481507T1 true ATE481507T1 (de) | 2010-10-15 |
Family
ID=34812082
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08075840T ATE491810T1 (de) | 2004-01-23 | 2005-01-21 | Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung |
| AT05712046T ATE481507T1 (de) | 2004-01-23 | 2005-01-21 | Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv |
| AT05722543T ATE417938T1 (de) | 2004-01-23 | 2005-01-21 | Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08075840T ATE491810T1 (de) | 2004-01-23 | 2005-01-21 | Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05722543T ATE417938T1 (de) | 2004-01-23 | 2005-01-21 | Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US7364854B2 (de) |
| EP (3) | EP2042612B1 (de) |
| JP (2) | JP2007518425A (de) |
| AT (3) | ATE491810T1 (de) |
| DE (3) | DE602005023586D1 (de) |
| WO (2) | WO2005070027A2 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2147118A4 (de) * | 2007-04-27 | 2010-09-01 | Eragen Biosciences Inc | Materialien und verfahren zum nachweis von hepatitis-c-virus |
| US8435741B2 (en) | 2007-07-26 | 2013-05-07 | Fujifilm Corporation | Isothermal nucleic acid amplification method using surfactant |
| US20090162856A1 (en) * | 2007-11-06 | 2009-06-25 | Hayato Miyoshi | Rna detection method |
| RU2011132045A (ru) * | 2008-12-31 | 2013-02-10 | Эбботт Лэборетриз | Праймеры и зонды для детектирования вируса гепатита с |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09107970A (ja) * | 1995-10-17 | 1997-04-28 | Soyaku Gijutsu Kenkyusho:Kk | Ｃ型肝炎ウイルス由来核酸、該核酸を用いたウイルスの検出方法 |
| EP0052296B1 (de) * | 1980-11-12 | 1984-06-27 | Mitsubishi Kasei Corporation | Als Heilmittel für Immunokrankheiten anwendbare Glutaminderivate, Verfahren zu deren Herstellung und Zusammensetzungen, die die obengenannten Derivate enthalten |
| US5087565A (en) * | 1984-07-02 | 1992-02-11 | Du Pont Merck Pharmaceutical Company | Synthetic process for preparation of 32 P-labeled nucleotides |
| DE3689223T2 (de) | 1985-05-15 | 1994-03-03 | Amoco Corp | Cytidinanaloga. |
| US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
| US5032511A (en) * | 1987-03-31 | 1991-07-16 | Mitsubishi Kasei Corporation | DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens |
| WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| US5714596A (en) * | 1987-11-18 | 1998-02-03 | Chiron Corporation | NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus |
| US5712088A (en) * | 1987-11-18 | 1998-01-27 | Chiron Corporation | Methods for detecting Hepatitis C virus using polynucleotides specific for same |
| US5698390A (en) * | 1987-11-18 | 1997-12-16 | Chiron Corporation | Hepatitis C immunoassays |
| US5030557A (en) | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| EP0578329B1 (de) | 1989-09-18 | 1996-03-13 | Canon Kabushiki Kaisha | Tintenstrahlaufzeichnungskopf und Tintenstrahlgerät mit diesem Kopf |
| DK0515506T3 (da) * | 1990-02-16 | 2000-05-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af carcinogene humane papillomavirus |
| KR100249110B1 (ko) | 1992-05-06 | 2000-04-01 | 다니엘 엘. 캐시앙 | 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트 |
| US5648232A (en) * | 1993-01-21 | 1997-07-15 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Microbiological best method and reagents |
| WO1995024499A1 (en) * | 1994-03-10 | 1995-09-14 | Gen-Probe Incorporated | Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents |
| US5874565A (en) * | 1995-08-29 | 1999-02-23 | Washington University | Nucleic acids comprising a highly conserved novel 3 terminal sequence element of the hepatitis C virus |
| US6127116A (en) * | 1995-08-29 | 2000-10-03 | Washington University | Functional DNA clone for hepatitis C virus (HCV) and uses thereof |
| DE19612779A1 (de) | 1996-03-29 | 1997-10-02 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR |
| EP2264045B1 (de) * | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren |
| DE19647055A1 (de) * | 1996-11-14 | 1998-05-20 | Boehringer Mannheim Gmbh | Stabilisierte wässrige Nukleosidtriphosphat-Lösungen |
| EP0975806B1 (de) * | 1997-04-03 | 2006-06-21 | Invitrogen Corporation | Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) |
| ZA989950B (en) | 1997-11-17 | 1999-05-04 | Akzo Nobel Nv | Transcription based amplification of double stranded DNA targets |
| JP2002512791A (ja) * | 1998-04-27 | 2002-05-08 | リボザイム・ファーマシューティカルズ・インコーポレーテッド | Ｃ型肝炎ウイルス感染に関連する疾患または状態の酵素的核酸治療 |
| AU4515699A (en) * | 1998-06-26 | 2000-01-17 | Akzo Nobel N.V. | Tagging of rna amplicons generated by transcription-based amplification |
| US6638714B1 (en) * | 1999-02-03 | 2003-10-28 | Ortho-Clinical Diagnostics, Inc. | Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof |
| US6300075B1 (en) * | 1999-02-03 | 2001-10-09 | Ortho-Clinical Diagnostics, Inc | Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid |
| WO2000075338A2 (en) * | 1999-06-04 | 2000-12-14 | The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services | CLONED GENONE OF INFECTIOUS HEPATITIS C VIRUS OF GENOTYPE 2a AND USES THEREOF |
| US6271004B1 (en) | 1999-06-25 | 2001-08-07 | Display Systems Biotech A/S | Method for improved reverse transcription at high temperatures |
| EP1233976A4 (de) * | 1999-11-17 | 2003-06-11 | Jiuping Ji | Simultaner nachweis von hbv, hcv und hiv in plasmaproben mit hilfe eines multiplexen fixierungstests |
| AU2001254670A1 (en) * | 2000-03-02 | 2001-09-12 | Akzo Nobel N.V. | Detection of hepatitis b virus rna |
| EP1383782A1 (de) * | 2001-03-26 | 2004-01-28 | Sirna Therpeutics, Inc. | Oligonukleotidvermittelte inhibierung der replikation des hepatitis--b-virus und des hepatitis-c-virus |
| WO2003016572A1 (en) * | 2001-08-17 | 2003-02-27 | Eli Lilly And Company | Oligonucleotide therapeutics for treating hepatitis c virus infections |
| US6783940B2 (en) * | 2001-10-31 | 2004-08-31 | Applera Corporation | Method of reducing non-specific amplification in PCR |
| US6750009B2 (en) * | 2002-01-29 | 2004-06-15 | Apath, Llc | Multiple viral replicon culture systems |
| WO2003100014A2 (en) * | 2002-05-24 | 2003-12-04 | Smithkline Beecham Corporation | Method for quantitating negative strand rna synthesis |
-
2005
- 2005-01-21 US US11/040,371 patent/US7364854B2/en not_active Expired - Lifetime
- 2005-01-21 EP EP08075840A patent/EP2042612B1/de not_active Expired - Lifetime
- 2005-01-21 DE DE602005023586T patent/DE602005023586D1/de not_active Expired - Lifetime
- 2005-01-21 AT AT08075840T patent/ATE491810T1/de not_active IP Right Cessation
- 2005-01-21 US US11/040,417 patent/US20060003343A1/en not_active Abandoned
- 2005-01-21 WO PCT/US2005/002414 patent/WO2005070027A2/en not_active Ceased
- 2005-01-21 DE DE602005011754T patent/DE602005011754D1/de not_active Expired - Lifetime
- 2005-01-21 EP EP05712046A patent/EP1721015B1/de not_active Expired - Lifetime
- 2005-01-21 AT AT05712046T patent/ATE481507T1/de not_active IP Right Cessation
- 2005-01-21 AT AT05722543T patent/ATE417938T1/de not_active IP Right Cessation
- 2005-01-21 EP EP05722543A patent/EP1706512B1/de not_active Expired - Lifetime
- 2005-01-21 JP JP2006551423A patent/JP2007518425A/ja active Pending
- 2005-01-21 DE DE602005025424T patent/DE602005025424D1/de not_active Expired - Lifetime
- 2005-01-21 JP JP2006551422A patent/JP4866248B2/ja not_active Expired - Fee Related
- 2005-01-21 WO PCT/US2005/002415 patent/WO2005071117A2/en not_active Ceased
-
2008
- 2008-02-11 US US12/069,606 patent/US7670779B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20060019266A1 (en) | 2006-01-26 |
| US7670779B2 (en) | 2010-03-02 |
| EP1721015A2 (de) | 2006-11-15 |
| WO2005070027A2 (en) | 2005-08-04 |
| WO2005071117A2 (en) | 2005-08-04 |
| JP4866248B2 (ja) | 2012-02-01 |
| US7364854B2 (en) | 2008-04-29 |
| DE602005011754D1 (de) | 2009-01-29 |
| EP2042612A3 (de) | 2009-06-17 |
| EP1721015B1 (de) | 2010-09-15 |
| EP2042612B1 (de) | 2010-12-15 |
| ATE417938T1 (de) | 2009-01-15 |
| WO2005070027A3 (en) | 2006-04-06 |
| WO2005070027A8 (en) | 2006-07-27 |
| US20060003343A1 (en) | 2006-01-05 |
| JP2007518424A (ja) | 2007-07-12 |
| JP2007518425A (ja) | 2007-07-12 |
| DE602005023586D1 (de) | 2010-10-28 |
| US20080248480A1 (en) | 2008-10-09 |
| EP1706512B1 (de) | 2008-12-17 |
| EP2042612A2 (de) | 2009-04-01 |
| ATE491810T1 (de) | 2011-01-15 |
| DE602005025424D1 (de) | 2011-01-27 |
| WO2005071117A3 (en) | 2006-04-20 |
| EP1706512A2 (de) | 2006-10-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |