ATE482286T1 - Verfahren zum nachweis einer mutation in einer nukleinsäure - Google Patents

Verfahren zum nachweis einer mutation in einer nukleinsäure

Info

Publication number
ATE482286T1
ATE482286T1 AT02725179T AT02725179T ATE482286T1 AT E482286 T1 ATE482286 T1 AT E482286T1 AT 02725179 T AT02725179 T AT 02725179T AT 02725179 T AT02725179 T AT 02725179T AT E482286 T1 ATE482286 T1 AT E482286T1
Authority
AT
Austria
Prior art keywords
nucleic acid
detecting
mutation
methods
target nucleic
Prior art date
Application number
AT02725179T
Other languages
English (en)
Inventor
Anthony Shuber
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Priority claimed from PCT/US2002/007926 external-priority patent/WO2002074995A1/en
Application granted granted Critical
Publication of ATE482286T1 publication Critical patent/ATE482286T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02725179T 2001-03-15 2002-03-15 Verfahren zum nachweis einer mutation in einer nukleinsäure ATE482286T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/809,713 US6428964B1 (en) 2001-03-15 2001-03-15 Method for alteration detection
US09/988,491 US6750020B2 (en) 2001-03-15 2001-11-20 Method for alteration detection
PCT/US2002/007926 WO2002074995A1 (en) 2001-03-15 2002-03-15 Method for alteration detection

Publications (1)

Publication Number Publication Date
ATE482286T1 true ATE482286T1 (de) 2010-10-15

Family

ID=25202052

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02725179T ATE482286T1 (de) 2001-03-15 2002-03-15 Verfahren zum nachweis einer mutation in einer nukleinsäure

Country Status (6)

Country Link
US (2) US6428964B1 (de)
EP (1) EP2267160A1 (de)
AT (1) ATE482286T1 (de)
DE (1) DE60237754D1 (de)
ES (1) ES2352417T3 (de)
PT (1) PT1368496E (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
AU2001217746A1 (en) 1998-05-14 2002-05-27 Calypso Medical, Inc. Systems and methods for locating and defining a target location within a human body
EP2289423A1 (de) * 1998-05-14 2011-03-02 David N. Krag System zum Klammern von Gewebe
EP1169479B1 (de) * 1999-04-09 2006-06-28 EXACT Sciences Corporation Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
CA2394921A1 (en) * 1999-12-07 2001-06-14 Anthony P. Shuber Supracolonic aerodigestive neoplasm detection
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6911308B2 (en) 2001-01-05 2005-06-28 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
US6428964B1 (en) * 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
EP1414840A4 (de) * 2001-03-27 2005-04-13 Univ Delaware Genomics-anwendungen für modifizierte oligonukleotide
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
US20040110179A1 (en) * 2002-03-15 2004-06-10 Shuber Anthony P. Method for alteration detection
US20040076606A1 (en) * 2002-09-14 2004-04-22 Ming-Shi Chang Methods of modulating inflammation by administration of interleukin-19 and inhibitors of IL-19 binding
US9740817B1 (en) 2002-10-18 2017-08-22 Dennis Sunga Fernandez Apparatus for biological sensing and alerting of pharmaco-genomic mutation
US20040086895A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Method of electrochemical detection of somatic cell mutations
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
US8346482B2 (en) * 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
WO2005108621A1 (en) * 2004-04-30 2005-11-17 Yale University Methods and compositions for cancer diagnosis
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
US7847274B2 (en) 2004-08-12 2010-12-07 Navotek Medical Ltd. Localization of a radioactive source within a body of a subject
US7952079B2 (en) 2004-08-12 2011-05-31 Navotek Medical Ltd. Localization of a radioactive source
US8239002B2 (en) * 2004-08-12 2012-08-07 Novatek Medical Ltd. Guiding a tool for medical treatment by detecting a source of radioactivity
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
CA2584723A1 (en) 2004-10-22 2006-05-04 Redpath Integrated Pathology, Inc. Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of dna
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
WO2006065598A2 (en) * 2004-12-13 2006-06-22 Geneohm Sciences, Inc. Fluidic cartridges for electrochemical detection of dna
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
CN101282760A (zh) * 2005-08-11 2008-10-08 纳沃特克医药有限公司 利用基于放射性的位置传感器的医疗系统和方法
US8058055B2 (en) * 2006-04-07 2011-11-15 Agilent Technologies, Inc. High resolution chromosomal mapping
GB0920014D0 (en) * 2009-11-13 2009-12-30 Medical Res Council Cell sampling device
CA3113672A1 (en) 2011-07-01 2013-01-10 Htg Molecular Diagnostics, Inc. Methods of detecting gene fusions
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US9341628B2 (en) 2012-11-30 2016-05-17 Interpace Diagnostics Corporation Methods for measuring carcinoembryonic antigen
US10253358B2 (en) 2013-11-04 2019-04-09 Exact Sciences Development Company, Llc Multiple-control calibrators for DNA quantitation
US10138524B2 (en) 2013-12-19 2018-11-27 Exact Sciences Development Company, Llc Synthetic nucleic acid control molecules
WO2018017710A1 (en) 2016-07-19 2018-01-25 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
EP3978624B1 (de) 2016-07-19 2025-03-26 Exact Sciences Corporation Methylierte kontroll-dna
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021195169A1 (en) 2020-03-24 2021-09-30 Fluent Biosciences Inc. Viral detection using template emulsification
EP4182477A4 (de) 2020-07-15 2024-09-11 Fluent Biosciences Inc. Abgestufte ligationsoligos
US20230002807A1 (en) * 2021-06-30 2023-01-05 Fluent Biosciences Inc. Methods and compositions for nucleic acid analysis
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4871838A (en) 1985-07-23 1989-10-03 The Board Of Rijks Universiteit Leiden Probes and methods for detecting activated ras oncogenes
US5348855A (en) 1986-03-05 1994-09-20 Miles Inc. Assay for nucleic acid sequences in an unpurified sample
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1341576C (en) 1986-08-11 2008-07-08 Thaddeus P. Dryja Diagnosis of retinoblastoma
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
GB8719061D0 (en) 1987-08-12 1987-09-16 Imp Cancer Research Fund Paten Probe
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
DE68916671T2 (de) 1988-03-18 1995-03-02 Baylor College Medicine Mutationsnachweis durch kompetitiven Oligonukleotid-Priming.
CA1339731C (en) 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
IE66572B1 (en) 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
EP0390323B2 (de) 1989-03-29 2012-08-08 Johns Hopkins University Nachweis des Ausfalls des Wildtyps des p53-Gens
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
US5589335A (en) 1989-04-05 1996-12-31 Amoco Corporation Hybridization promotion reagents
JP2802125B2 (ja) 1989-06-23 1998-09-24 キヤノン株式会社 核酸の検出方法
DK0407789T3 (da) 1989-06-23 1993-11-01 Canon Kk Fremgangsmåde til detektering af nukleinsyrer
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
ES2115611T3 (es) 1990-01-04 1998-07-01 Univ Johns Hopkins Gen suprimido en el cancer colorrectal humano.
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
IL97222A (en) 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and reagent for determining specific nucleotide variations
US5126239A (en) 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
DK0489149T3 (da) 1990-06-27 2001-12-27 Univ Princeton Prober til påvisning af mutant P53
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
IE66125B1 (en) 1991-01-31 1995-12-13 Zeneca Ltd Detection method for variant nucleotides
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
AU3919293A (en) 1992-03-27 1993-11-08 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
US5645986A (en) 1992-05-13 1997-07-08 Board Of Reagents, The University Of Texas System Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
US5489508A (en) 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
US5633134A (en) 1992-10-06 1997-05-27 Ig Laboratories, Inc. Method for simultaneously detecting multiple mutations in a DNA sample
US5436142A (en) 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
US5888780A (en) 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
WO1995002068A1 (en) 1993-07-09 1995-01-19 Wakunaga Seiyaku Kabushiki Kaisha Method of discriminating nucleic acid and testing set for discriminating nucleic acid
FR2709761B1 (fr) 1993-09-10 1995-11-24 Pasteur Institut Procédé de détection de molécules contenant des mésappariements nucléotidiques et de localisation de ces mésappariements, et application à la détection de substitutions ou de délétions de bases .
WO1995009928A1 (en) 1993-10-06 1995-04-13 The Regents Of The University Of California Methods for detecting cancer-associated chromosome deletions using in situ hybridization
ES2240970T3 (es) 1993-11-03 2005-10-16 Orchid Biosciences, Inc. Polimorfismos de nucleotido simple y su uso en analisis geneticos.
US6025127A (en) * 1994-01-14 2000-02-15 The Johns Hopkins University School Of Medicine Nucleic acid mutation detection in histologic tissue
AU693198B2 (en) 1994-04-25 1998-06-25 Avitech Diagnostics, Inc. Detection of mutation by resolvase cleavage
JPH10500310A (ja) * 1994-05-19 1998-01-13 ダコ アクティーゼルスカブ 淋菌及びトラコーマ クラミジアの検出のためのｐｎａ プローブ
US5545527A (en) 1994-07-08 1996-08-13 Visible Genetics Inc. Method for testing for mutations in DNA from a patient sample
US5589330A (en) 1994-07-28 1996-12-31 Genzyme Corporation High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides
US5849483A (en) 1994-07-28 1998-12-15 Ig Laboratories, Inc. High throughput screening method for sequences or genetic alterations in nucleic acids
US5834181A (en) 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
US5702886A (en) 1994-10-05 1997-12-30 The Johns Hopkins University Chromosome I8Q loss and prognosis in colorectal cancer
JPH10507366A (ja) 1994-10-21 1998-07-21 ゼネカ・リミテッド Ｐｃｒによるテロメアｄｎａの可変性の特性決定法
US5629178A (en) 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
US5512441A (en) 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
US5707806A (en) 1995-06-07 1998-01-13 Genzyme Corporation Direct sequence identification of mutations by cleavage- and ligation-associated mutation-specific sequencing
US5571676A (en) 1995-06-07 1996-11-05 Ig Laboratories, Inc. Method for mismatch-directed in vitro DNA sequencing
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5670325A (en) 1996-08-14 1997-09-23 Exact Laboratories, Inc. Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample
ES2220997T3 (es) 1995-12-22 2004-12-16 Exact Sciences Corporation Metodos para la deteccion de poblaciones clonales de celulas transformadas en una muestra celular genomicamente heterogenea.
US5741650A (en) 1996-01-30 1998-04-21 Exact Laboratories, Inc. Methods for detecting colon cancer from stool samples
AU2069597A (en) * 1996-03-04 1997-09-22 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US5811239A (en) 1996-05-13 1998-09-22 Frayne Consultants Method for single base-pair DNA sequence variation detection
US6100029A (en) 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US6146828A (en) 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6143529A (en) 1996-08-14 2000-11-07 Exact Laboratories, Inc. Methods for improving sensitivity and specificity of screening assays
US5928870A (en) 1997-06-16 1999-07-27 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
US6300077B1 (en) 1996-08-14 2001-10-09 Exact Sciences Corporation Methods for the detection of nucleic acids
US6203993B1 (en) 1996-08-14 2001-03-20 Exact Science Corp. Methods for the detection of nucleic acids
US5952178A (en) 1996-08-14 1999-09-14 Exact Laboratories Methods for disease diagnosis from stool samples
US6020137A (en) 1996-08-14 2000-02-01 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
EP0975797A1 (de) 1997-02-28 2000-02-02 Exact Laboratories, Inc. Verfahren zur analyse von nukleinsäuren
US5830665A (en) 1997-03-03 1998-11-03 Exact Laboratories, Inc. Contiguous genomic sequence scanning
US6048689A (en) 1997-03-28 2000-04-11 Gene Logic, Inc. Method for identifying variations in polynucleotide sequences
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US20020004201A1 (en) 1997-06-16 2002-01-10 Lapidus Stanley N. Methods for the detection of loss of heterozygosity
AU7816898A (en) 1997-06-16 1999-01-04 Exact Laboratories, Inc. Methods for stool sample preparation
US5888778A (en) 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US6818404B2 (en) 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
JP2001520054A (ja) 1997-10-23 2001-10-30 エグザクト サイエンシーズ コーポレイション Ｐｃｒを用いる分子診断におけるコンタミネーションを検出するための方法
US20020001800A1 (en) 1998-08-14 2002-01-03 Stanley N. Lapidus Diagnostic methods using serial testing of polymorphic loci
WO2000031298A1 (en) 1998-11-23 2000-06-02 Exact Laboratories, Inc. Methods for detecting lower-frequency molecules
CA2352456A1 (en) 1998-11-23 2000-06-02 Exact Sciences Corporation Primer extension methods utilizing donor and acceptor molecules for detecting nucleic acids
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
AU752817B2 (en) 1999-02-25 2002-10-03 Exact Sciences Corporation Methods for preserving DNA integrity
AU4669600A (en) 1999-05-03 2000-11-17 Exact Laboratories, Inc. Stool specimen collector
US20010039012A1 (en) 1999-06-14 2001-11-08 Lapidus Stanley N. Methods for diagnostic screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6911308B2 (en) 2001-01-05 2005-06-28 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
US6428964B1 (en) * 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US20030049659A1 (en) 2001-05-29 2003-03-13 Lapidus Stanley N. Devices and methods for isolating samples into subsamples for analysis

Also Published As

Publication number Publication date
US6750020B2 (en) 2004-06-15
US20020132251A1 (en) 2002-09-19
DE60237754D1 (de) 2010-11-04
EP2267160A1 (de) 2010-12-29
ES2352417T3 (es) 2011-02-18
PT1368496E (pt) 2010-12-14
US6428964B1 (en) 2002-08-06

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