ATE486961T1 - Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) - Google Patents

Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr)

Info

Publication number
ATE486961T1
ATE486961T1 AT06012490T AT06012490T ATE486961T1 AT E486961 T1 ATE486961 T1 AT E486961T1 AT 06012490 T AT06012490 T AT 06012490T AT 06012490 T AT06012490 T AT 06012490T AT E486961 T1 ATE486961 T1 AT E486961T1
Authority
AT
Austria
Prior art keywords
pcr
methods
amplification
molecules
chain reaction
Prior art date
Application number
AT06012490T
Other languages
English (en)
Inventor
Jun E Lee
Ayoub Rashtchian
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE486961T1 publication Critical patent/ATE486961T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT06012490T 1997-04-03 1998-04-03 Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) ATE486961T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4262997P 1997-04-03 1997-04-03

Publications (1)

Publication Number Publication Date
ATE486961T1 true ATE486961T1 (de) 2010-11-15

Family

ID=21922934

Family Applications (2)

Application Number Title Priority Date Filing Date
AT98917984T ATE331040T1 (de) 1997-04-03 1998-04-03 Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
AT06012490T ATE486961T1 (de) 1997-04-03 1998-04-03 Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr)

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT98917984T ATE331040T1 (de) 1997-04-03 1998-04-03 Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)

Country Status (8)

Country Link
US (6) US6495350B1 (de)
EP (3) EP1705253B1 (de)
AT (2) ATE331040T1 (de)
AU (1) AU7100198A (de)
DE (2) DE69841990D1 (de)
DK (1) DK1705253T3 (de)
ES (1) ES2355739T3 (de)
WO (1) WO1998044161A1 (de)

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US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6300069B1 (en) * 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
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WO2003025132A2 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
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US20040157213A1 (en) * 2003-01-27 2004-08-12 Kennell John C. Fungal reverse transcriptases with enhanced capabilities
EP1594982A4 (de) * 2003-01-29 2006-04-12 Quanta Biosciences Inc Verfahren und zusammensetzungen zum nachweis und zur quantifizierung von rna
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
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EP1706512B1 (de) * 2004-01-23 2008-12-17 BioMerieux, Inc. Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren
EP1807512A4 (de) * 2004-04-06 2008-12-31 Stratagene California Kompositionen und verfahren zur rücktranskription
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US20060024722A1 (en) * 2004-07-30 2006-02-02 Mark Fischer-Colbrie Samples for detection of oncofetal fibronectin and uses thereof
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US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
JP5702148B2 (ja) * 2007-11-27 2015-04-15 バイオ−ラッド ラボラトリーズ，インコーポレイティド １ステップｒｔ−ｐｃｒの阻害低減
JP5279339B2 (ja) * 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物
BRPI0908748A2 (pt) 2008-03-15 2015-07-28 Hologic Inc Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
WO2011163120A1 (en) * 2010-06-21 2011-12-29 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
WO2012054727A1 (en) 2010-10-22 2012-04-26 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
WO2012150317A1 (en) * 2011-05-05 2012-11-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
EP3461807B1 (de) 2011-06-08 2023-07-12 Life Technologies Corporation Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP2559764A1 (de) * 2011-08-17 2013-02-20 Qiagen GmbH Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer
EP3415638B1 (de) 2012-10-18 2025-10-08 IDEXX Laboratories, Inc. Nukleinsäureamplifikationssteuerungen sowie -kits und verfahren zur verwendung davon
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
EP3478702A4 (de) 2016-06-27 2020-03-18 Dana-Farber Cancer Institute, Inc. Verfahren zur messung der rna-translationsraten
WO2018136404A1 (en) * 2017-01-19 2018-07-26 Asuragen, Inc. Methods of rna amplification
WO2019118652A1 (en) 2017-12-12 2019-06-20 Essenlix Corporation Sample manipulation and assay with rapid temperature change
US11453907B2 (en) 2020-03-23 2022-09-27 The Broad Institute, Inc. Crispr effector system based coronavirus diagnostics
WO2022018685A1 (en) 2020-07-22 2022-01-27 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
EP4363611A1 (de) 2021-06-30 2024-05-08 Dana-Farber Cancer Institute, Inc. Zusammensetzungen und verfahren zur anreicherung von nukleinsäuren mit lichtvermittelter vernetzung

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Also Published As

Publication number Publication date
US20030113712A1 (en) 2003-06-19
US20110217735A1 (en) 2011-09-08
US6767724B2 (en) 2004-07-27
EP2354251A1 (de) 2011-08-10
ATE331040T1 (de) 2006-07-15
AU7100198A (en) 1998-10-22
US20040219595A1 (en) 2004-11-04
US8372604B2 (en) 2013-02-12
DE69835008T2 (de) 2007-01-11
DE69835008D1 (de) 2006-08-03
US6495350B1 (en) 2002-12-17
EP1705253A2 (de) 2006-09-27
EP1705253A3 (de) 2006-12-27
EP0975806A1 (de) 2000-02-02
US9873901B2 (en) 2018-01-23
DK1705253T3 (da) 2011-02-21
DE69841990D1 (de) 2010-12-16
WO1998044161A1 (en) 1998-10-08
US9556466B2 (en) 2017-01-31
US20130164788A1 (en) 2013-06-27
EP0975806A4 (de) 2004-10-06
US20150017688A1 (en) 2015-01-15
EP2354251B1 (de) 2014-10-15
EP0975806B1 (de) 2006-06-21
ES2355739T3 (es) 2011-03-30
US7883871B2 (en) 2011-02-08
EP1705253B1 (de) 2010-11-03

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