ATE486961T1 - Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) - Google Patents

Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr)

Info

Publication number
ATE486961T1
ATE486961T1 AT06012490T AT06012490T ATE486961T1 AT E486961 T1 ATE486961 T1 AT E486961T1 AT 06012490 T AT06012490 T AT 06012490T AT 06012490 T AT06012490 T AT 06012490T AT E486961 T1 ATE486961 T1 AT E486961T1
Authority
AT
Austria
Prior art keywords
pcr
methods
amplification
molecules
chain reaction
Prior art date
Application number
AT06012490T
Other languages
English (en)
Inventor
Jun E Lee
Ayoub Rashtchian
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE486961T1 publication Critical patent/ATE486961T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT06012490T 1997-04-03 1998-04-03 Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) ATE486961T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4262997P 1997-04-03 1997-04-03

Publications (1)

Publication Number Publication Date
ATE486961T1 true ATE486961T1 (de) 2010-11-15

Family

ID=21922934

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06012490T ATE486961T1 (de) 1997-04-03 1998-04-03 Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr)
AT98917984T ATE331040T1 (de) 1997-04-03 1998-04-03 Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT98917984T ATE331040T1 (de) 1997-04-03 1998-04-03 Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)

Country Status (8)

Country Link
US (6) US6495350B1 (de)
EP (3) EP2354251B1 (de)
AT (2) ATE486961T1 (de)
AU (1) AU7100198A (de)
DE (2) DE69835008T2 (de)
DK (1) DK1705253T3 (de)
ES (1) ES2355739T3 (de)
WO (1) WO1998044161A1 (de)

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US20040157213A1 (en) * 2003-01-27 2004-08-12 Kennell John C. Fungal reverse transcriptases with enhanced capabilities
EP1594982A4 (de) * 2003-01-29 2006-04-12 Quanta Biosciences Inc Verfahren und zusammensetzungen zum nachweis und zur quantifizierung von rna
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
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JP5106416B2 (ja) * 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮(packed)DNAポリメラーゼを含む核酸複製のための反応緩衝液組成物
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US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8338094B2 (en) 2007-11-27 2012-12-25 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step RT-PCR
JP5279339B2 (ja) * 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物
CN102301000B (zh) 2008-03-15 2015-04-29 豪洛捷公司 核酸分子扩增反应的分析组分及方法
EP3502272A1 (de) 2010-06-21 2019-06-26 Life Technologies Corporation Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren
DK2582839T3 (en) 2010-06-21 2016-08-29 Life Technologies Corp Compositions, methods and kits for nucleic acid synthesis and amplification by RT
WO2012054727A1 (en) * 2010-10-22 2012-04-26 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
WO2012150317A1 (en) * 2011-05-05 2012-11-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP2559764A1 (de) * 2011-08-17 2013-02-20 Qiagen GmbH Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer
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EP3478702A4 (de) 2016-06-27 2020-03-18 Dana-Farber Cancer Institute, Inc. Verfahren zur messung der rna-translationsraten
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US11648551B2 (en) 2017-12-12 2023-05-16 Essenlix Corporation Sample manipulation and assay with rapid temperature change
US11639523B2 (en) 2020-03-23 2023-05-02 The Broad Institute, Inc. Type V CRISPR-Cas systems and use thereof
US20230265531A1 (en) 2020-07-22 2023-08-24 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
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Also Published As

Publication number Publication date
DE69835008D1 (de) 2006-08-03
ES2355739T3 (es) 2011-03-30
US20040219595A1 (en) 2004-11-04
EP2354251A1 (de) 2011-08-10
EP2354251B1 (de) 2014-10-15
EP0975806A4 (de) 2004-10-06
US6495350B1 (en) 2002-12-17
EP1705253A2 (de) 2006-09-27
US9873901B2 (en) 2018-01-23
DE69835008T2 (de) 2007-01-11
EP1705253A3 (de) 2006-12-27
WO1998044161A1 (en) 1998-10-08
DE69841990D1 (de) 2010-12-16
EP1705253B1 (de) 2010-11-03
AU7100198A (en) 1998-10-22
US6767724B2 (en) 2004-07-27
EP0975806B1 (de) 2006-06-21
DK1705253T3 (da) 2011-02-21
ATE331040T1 (de) 2006-07-15
US9556466B2 (en) 2017-01-31
US20150017688A1 (en) 2015-01-15
US20110217735A1 (en) 2011-09-08
EP0975806A1 (de) 2000-02-02
US20130164788A1 (en) 2013-06-27
US8372604B2 (en) 2013-02-12
US7883871B2 (en) 2011-02-08
US20030113712A1 (en) 2003-06-19

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