DK1705253T3 - Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR) - Google Patents

Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)

Info

Publication number
DK1705253T3
DK1705253T3 DK06012490.6T DK06012490T DK1705253T3 DK 1705253 T3 DK1705253 T3 DK 1705253T3 DK 06012490 T DK06012490 T DK 06012490T DK 1705253 T3 DK1705253 T3 DK 1705253T3
Authority
DK
Denmark
Prior art keywords
reverse transcriptase
pcr
methods
amplification
molecules
Prior art date
Application number
DK06012490.6T
Other languages
English (en)
Inventor
Jun E Lee
Ayoub Rashtchian
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of DK1705253T3 publication Critical patent/DK1705253T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK06012490.6T 1997-04-03 1998-04-03 Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR) DK1705253T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4262997P 1997-04-03 1997-04-03
EP98917984A EP0975806B1 (en) 1997-04-03 1998-04-03 Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)

Publications (1)

Publication Number Publication Date
DK1705253T3 true DK1705253T3 (da) 2011-02-21

Family

ID=21922934

Family Applications (1)

Application Number Title Priority Date Filing Date
DK06012490.6T DK1705253T3 (da) 1997-04-03 1998-04-03 Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)

Country Status (8)

Country Link
US (6) US6495350B1 (da)
EP (3) EP0975806B1 (da)
AT (2) ATE486961T1 (da)
AU (1) AU7100198A (da)
DE (2) DE69835008T2 (da)
DK (1) DK1705253T3 (da)
ES (1) ES2355739T3 (da)
WO (1) WO1998044161A1 (da)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593120B1 (en) 1994-04-01 2003-07-15 Gen-Probe Incorporated Recombinant DNA encoding a reverse transcriptase derived from moloney murine leukemia virus
ES2355739T3 (es) 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
EP1005481B1 (en) 1997-04-22 2009-10-14 Life Technologies Corporation Methods for the production of aslv reverse transcriptases composed of multiple subunits
US7122304B2 (en) 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
US20040185493A1 (en) * 1997-05-12 2004-09-23 Invitrogen Corporation Methods for production and purification of nucleic acid molecules
AU3534199A (en) * 1998-04-23 1999-11-08 Takara Shuzo Co., Ltd. Method For Synthesizing DNA
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6300069B1 (en) 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
JP2003500070A (ja) * 1999-05-21 2003-01-07 インビトロゲン・コーポレーション 核酸分子を標識するための組成物および方法
EP1436385A4 (en) * 2001-09-14 2005-12-14 Invitrogen Corp DNA POLYMERASES AND MUTANTS CORRESPONDING
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
ES2579803T3 (es) * 2002-09-03 2016-08-16 Quanta Biosciences, Inc. Composiciones y métodos mejorados para la síntesis de ADNc
US20040157213A1 (en) * 2003-01-27 2004-08-12 Kennell John C. Fungal reverse transcriptases with enhanced capabilities
EP1594982A4 (en) * 2003-01-29 2006-04-12 Quanta Biosciences Inc METHODS AND COMPOSITIONS FOR RNA DETECTION AND QUANTIFICATION
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
JP2007518425A (ja) * 2004-01-23 2007-07-12 バイオメリュー・インコーポレイテッド Ｈｃｖ３’非翻訳領域を効率的に増幅および検出するためのプライマーおよびプローブの設計
AU2005232644A1 (en) * 2004-04-06 2005-10-27 Stratagen California Compositions and methods for reverse transcription
US7566532B1 (en) 2004-07-02 2009-07-28 Quest Diagnostics Investments Incorporated Methods for detecting retroviruses
US20060024722A1 (en) 2004-07-30 2006-02-02 Mark Fischer-Colbrie Samples for detection of oncofetal fibronectin and uses thereof
JP2008518621A (ja) * 2004-11-03 2008-06-05 サード・ウェーブ・テクノロジーズ・インク 一段階検出アッセイ法
EP1989318B1 (en) * 2006-01-06 2014-07-30 Agilent Technologies, Inc. Reaction buffer composition for nucleic acid replication with packed dna polymerases
EP3167900B1 (en) 2006-03-29 2018-11-21 Merial Limited Vaccine against streptococci
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2009070604A1 (en) * 2007-11-27 2009-06-04 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step rt-pcr
JP5279339B2 (ja) * 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物
KR101419980B1 (ko) 2008-03-15 2014-07-15 홀로직, 인크. 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법
ES2908415T3 (es) * 2010-06-21 2022-04-29 Life Technologies Corp Composición y método para la síntesis y amplificación de ácidos nucleicos mediante el uso de RT
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
EP2705156B1 (en) * 2011-05-05 2015-08-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Linear dna amplification
DK2718260T3 (da) 2011-06-08 2018-11-19 Life Technologies Corp Design og udvikling af nye detergenter til brug i pcr systemer
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP2559764A1 (en) 2011-08-17 2013-02-20 Qiagen GmbH Composition and methods for RT-PCR comprising an anionic polymer
DK2909339T3 (da) 2012-10-18 2018-08-13 Idexx Lab Inc Kits omfattende kontroller for nukleinsyreamplifikation
CN106458885B (zh) 2013-10-25 2019-12-10 生命技术公司 用于聚合酶链式反应系统的新颖化合物及其应用
US11155857B2 (en) 2016-06-27 2021-10-26 Dana-Farber Cancer Institute, Inc. Methods for measuring RNA translation rates
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
US11648551B2 (en) 2017-12-12 2023-05-16 Essenlix Corporation Sample manipulation and assay with rapid temperature change
US11639523B2 (en) 2020-03-23 2023-05-02 The Broad Institute, Inc. Type V CRISPR-Cas systems and use thereof
WO2022018685A1 (en) 2020-07-22 2022-01-27 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
US20240287594A1 (en) 2021-06-30 2024-08-29 Dana-Farber Cancer Institute, Inc. Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5407800A (en) 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5618702A (en) 1988-08-19 1997-04-08 City Of Hope PCR amplification of mRNA
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
EP0894860B1 (en) 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostable DNA polymerases and deletion mutants thereof
FR2690691B1 (fr) 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
USRE39031E1 (en) 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
JPH08131171A (ja) 1994-11-11 1996-05-28 Asahi Chem Ind Co Ltd 逆転写酵素の安定化組成物
EP0736608A1 (de) * 1995-04-08 1996-10-09 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
ES2355739T3 (es) * 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
EP1005481B1 (en) 1997-04-22 2009-10-14 Life Technologies Corporation Methods for the production of aslv reverse transcriptases composed of multiple subunits
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6630333B1 (en) * 1999-03-23 2003-10-07 Invitrogen Corporation Substantially pure reverse transriptases and methods of production thereof
US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US8278083B2 (en) 2004-03-22 2012-10-02 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Inactivated influenza virus compositions

Also Published As

Publication number Publication date
AU7100198A (en) 1998-10-22
EP2354251B1 (en) 2014-10-15
EP1705253A3 (en) 2006-12-27
US20030113712A1 (en) 2003-06-19
ES2355739T3 (es) 2011-03-30
EP2354251A1 (en) 2011-08-10
US6495350B1 (en) 2002-12-17
US9873901B2 (en) 2018-01-23
EP0975806A4 (en) 2004-10-06
US6767724B2 (en) 2004-07-27
US8372604B2 (en) 2013-02-12
ATE331040T1 (de) 2006-07-15
US9556466B2 (en) 2017-01-31
EP0975806A1 (en) 2000-02-02
EP0975806B1 (en) 2006-06-21
US20040219595A1 (en) 2004-11-04
US20130164788A1 (en) 2013-06-27
US7883871B2 (en) 2011-02-08
ATE486961T1 (de) 2010-11-15
WO1998044161A1 (en) 1998-10-08
DE69841990D1 (de) 2010-12-16
EP1705253A2 (en) 2006-09-27
US20150017688A1 (en) 2015-01-15
DE69835008D1 (de) 2006-08-03
DE69835008T2 (de) 2007-01-11
EP1705253B1 (en) 2010-11-03
US20110217735A1 (en) 2011-09-08

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