ATE491811T1 - Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr - Google Patents
Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcrInfo
- Publication number
- ATE491811T1 ATE491811T1 AT07820522T AT07820522T ATE491811T1 AT E491811 T1 ATE491811 T1 AT E491811T1 AT 07820522 T AT07820522 T AT 07820522T AT 07820522 T AT07820522 T AT 07820522T AT E491811 T1 ATE491811 T1 AT E491811T1
- Authority
- AT
- Austria
- Prior art keywords
- pcr
- real
- combination
- rems
- mutant alleles
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 108700028369 Alleles Proteins 0.000 title 1
- 238000003753 real-time PCR Methods 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- LNEPOXFFQSENCJ-UHFFFAOYSA-N haloperidol Chemical compound C1CC(O)(C=2C=CC(Cl)=CC=2)CCN1CCCC(=O)C1=CC=C(F)C=C1 LNEPOXFFQSENCJ-UHFFFAOYSA-N 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06020128A EP1905842A1 (de) | 2006-09-26 | 2006-09-26 | Verfahren zum Nachweis des mutierten Alleles mittels Real Time PCR und REMS-PCR |
| PCT/EP2007/060116 WO2008037694A1 (en) | 2006-09-26 | 2007-09-24 | Method for detection of mutant alleles combining real time pcr and rems-pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE491811T1 true ATE491811T1 (de) | 2011-01-15 |
Family
ID=37687373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07820522T ATE491811T1 (de) | 2006-09-26 | 2007-09-24 | Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8329405B2 (de) |
| EP (2) | EP1905842A1 (de) |
| AT (1) | ATE491811T1 (de) |
| CA (1) | CA2666442A1 (de) |
| DE (1) | DE602007011301D1 (de) |
| ES (1) | ES2354745T3 (de) |
| WO (1) | WO2008037694A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090068643A1 (en) * | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
| US8999634B2 (en) * | 2007-04-27 | 2015-04-07 | Quest Diagnostics Investments Incorporated | Nucleic acid detection combining amplification with fragmentation |
| EP2376659B1 (de) | 2008-12-17 | 2015-12-02 | Life Technologies Corporation | Verfahren, zusammensetzungen und kits für den nachweis von allelen varianten |
| EP2411543B1 (de) | 2009-03-27 | 2017-04-19 | Life Technologies Corporation | Verfahren, zusammensetzungen und kits für den nachweis von allelen varianten |
| CN102115782B (zh) * | 2009-12-30 | 2014-06-18 | 北京雅康博生物科技有限公司 | 用于定量检测K-ras突变的试剂盒 |
| CN103930571A (zh) * | 2011-11-17 | 2014-07-16 | 瑞尼克斯有限公司 | 用于选择性分子分析的系统和方法 |
| US9133509B2 (en) | 2012-03-22 | 2015-09-15 | Lgc Genomics Limited | Polymerase chain reaction detection system |
| CN103571947B (zh) * | 2013-10-09 | 2015-03-25 | 武汉百泰基因工程有限公司 | 一种用于检测kras基因热点突变的试剂盒 |
| US10433030B2 (en) | 2014-10-09 | 2019-10-01 | Thuuz, Inc. | Generating a customized highlight sequence depicting multiple events |
| CN106048015A (zh) * | 2016-06-07 | 2016-10-26 | 中国疾病预防控制中心传染病预防控制所 | 限制性内切酶介导的依赖于pcr扩增的实时核酸检测技术 |
| CN107937524A (zh) * | 2017-12-05 | 2018-04-20 | 中源协和基因科技有限公司 | 人类kras基因突变检测试剂盒及检测方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9725237D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| AUPQ495700A0 (en) * | 2000-01-05 | 2000-02-03 | Johnson & Johnson Research Pty. Limited | Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences |
| CA2594223C (en) * | 2005-01-03 | 2016-03-15 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primer for nucleic acid detection |
| EP1969140A2 (de) | 2005-11-23 | 2008-09-17 | DiaSorin S.p.A. | Reagentien und verfahren zur gleichzeitigen nukleinsäureamplifikation und -detektion |
-
2006
- 2006-09-26 EP EP06020128A patent/EP1905842A1/de not_active Withdrawn
-
2007
- 2007-09-24 EP EP07820522A patent/EP2066804B1/de not_active Not-in-force
- 2007-09-24 CA CA002666442A patent/CA2666442A1/en not_active Abandoned
- 2007-09-24 AT AT07820522T patent/ATE491811T1/de not_active IP Right Cessation
- 2007-09-24 US US12/443,175 patent/US8329405B2/en active Active
- 2007-09-24 DE DE602007011301T patent/DE602007011301D1/de active Active
- 2007-09-24 WO PCT/EP2007/060116 patent/WO2008037694A1/en not_active Ceased
- 2007-09-24 ES ES07820522T patent/ES2354745T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US8329405B2 (en) | 2012-12-11 |
| ES2354745T3 (es) | 2011-03-17 |
| US20100190157A1 (en) | 2010-07-29 |
| DE602007011301D1 (de) | 2011-01-27 |
| WO2008037694A1 (en) | 2008-04-03 |
| EP2066804B1 (de) | 2010-12-15 |
| CA2666442A1 (en) | 2008-04-03 |
| EP1905842A1 (de) | 2008-04-02 |
| EP2066804A1 (de) | 2009-06-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |