ATE528395T1 - In an den 5'-terminus eines primers zur verwendung bei einer nukleinsäureamplifikationsreaktion gebundener form verwendetes dna-fragment und verwendung davon - Google Patents

In an den 5'-terminus eines primers zur verwendung bei einer nukleinsäureamplifikationsreaktion gebundener form verwendetes dna-fragment und verwendung davon

Info

Publication number
ATE528395T1
ATE528395T1 AT07806129T AT07806129T ATE528395T1 AT E528395 T1 ATE528395 T1 AT E528395T1 AT 07806129 T AT07806129 T AT 07806129T AT 07806129 T AT07806129 T AT 07806129T AT E528395 T1 ATE528395 T1 AT E528395T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid amplification
dna fragment
primer
amplification reaction
Prior art date
Application number
AT07806129T
Other languages
English (en)
Inventor
Kazuhiko Nakatani
Fumie Takei
Masaki Hagihara
Original Assignee
Univ Osaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Osaka filed Critical Univ Osaka
Application granted granted Critical
Publication of ATE528395T1 publication Critical patent/ATE528395T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT07806129T 2006-09-01 2007-08-28 In an den 5'-terminus eines primers zur verwendung bei einer nukleinsäureamplifikationsreaktion gebundener form verwendetes dna-fragment und verwendung davon ATE528395T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006238299 2006-09-01
PCT/JP2007/066650 WO2008026582A1 (fr) 2006-09-01 2007-08-28 Fragment d'adn utilisé dans la forme attachée à l'extrémité 5' de l'amorce pour une utilisation dans une réaction d'amplification d'un acide nucléique, et son utilisation

Publications (1)

Publication Number Publication Date
ATE528395T1 true ATE528395T1 (de) 2011-10-15

Family

ID=39135865

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07806129T ATE528395T1 (de) 2006-09-01 2007-08-28 In an den 5'-terminus eines primers zur verwendung bei einer nukleinsäureamplifikationsreaktion gebundener form verwendetes dna-fragment und verwendung davon

Country Status (5)

Country Link
US (1) US8911947B2 (de)
EP (1) EP2058392B1 (de)
JP (1) JP4873427B2 (de)
AT (1) ATE528395T1 (de)
WO (1) WO2008026582A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP5692774B2 (ja) * 2010-03-11 2015-04-01 国立大学法人大阪大学 一塩基多型を検出する方法および試薬キット
WO2012051152A2 (en) * 2010-10-12 2012-04-19 Kevin Ryan Novel dna templates for small rna production in mammalian cells
WO2013133402A1 (ja) * 2012-03-08 2013-09-12 株式会社古河電工アドバンストエンジニアリング 核酸中の一塩基多型の検出方法
JP6440997B2 (ja) * 2014-08-22 2018-12-19 国立大学法人大阪大学 Ｐｃｒ法およびｐｃｒキット
DE112016000293T5 (de) 2015-02-26 2017-09-21 Hitachi High-Technologies Corporation Verfahren zum konstruieren eines nucleinsäure-moleküls

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4413325B2 (ja) 1999-09-16 2010-02-10 独立行政法人科学技術振興機構 バルジ塩基認識分子及びそれを含有するｄｎａ
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US20030148301A1 (en) * 1999-12-10 2003-08-07 Toshiya Aono Method of detecting nucleotide polymorphism
WO2001042498A1 (en) 1999-12-10 2001-06-14 Toyo Boseki Kabushiki Kaisha Method of detecting nucleotide polymorphism
US20020182622A1 (en) * 2001-02-01 2002-12-05 Yusuke Nakamura Method for SNP (single nucleotide polymorphism) typing
JP2002300894A (ja) 2001-02-01 2002-10-15 Inst Of Physical & Chemical Res 一塩基多型タイピング方法
JP2003052372A (ja) 2001-07-27 2003-02-25 Unitech Kk 簡易ｓｎｐ検出法（ｓｓｄ）
US20060014144A1 (en) * 2001-12-18 2006-01-19 Christensen Ulf B Pseudonucleotide comprising an intercalator
JP2003259873A (ja) 2002-03-07 2003-09-16 Japan Science & Technology Corp バルジ塩基検出分子
JP4177006B2 (ja) 2002-03-08 2008-11-05 独立行政法人科学技術振興機構 グアニンバルジ修飾分子
US20070059693A1 (en) * 2003-01-02 2007-03-15 University Of Rochester Hybridization-based biosensor containing hairpin probes and use thereof
JP4464614B2 (ja) 2003-02-28 2010-05-19 独立行政法人科学技術振興機構 バルジ塩基認識分子およびバルジ塩基検出方法
US7671184B2 (en) 2004-09-24 2010-03-02 Universitact Bern Molecular beacons
JP4752071B2 (ja) 2005-02-01 2011-08-17 国立大学法人京都大学 一塩基多型の検出方法
CA2597330A1 (en) 2005-02-09 2006-08-17 Stratagene California Key probe compositions and methods for polynucleotide detection

Also Published As

Publication number Publication date
US20100015618A1 (en) 2010-01-21
US8911947B2 (en) 2014-12-16
EP2058392A4 (de) 2010-09-22
JP4873427B2 (ja) 2012-02-08
EP2058392A1 (de) 2009-05-13
JPWO2008026582A1 (ja) 2010-01-21
WO2008026582A1 (fr) 2008-03-06
EP2058392B1 (de) 2011-10-12

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