ATE494392T1 - Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) - Google Patents

Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)

Info

Publication number
ATE494392T1
ATE494392T1 AT05810351T AT05810351T ATE494392T1 AT E494392 T1 ATE494392 T1 AT E494392T1 AT 05810351 T AT05810351 T AT 05810351T AT 05810351 T AT05810351 T AT 05810351T AT E494392 T1 ATE494392 T1 AT E494392T1
Authority
AT
Austria
Prior art keywords
mirnas
target polynucleotide
rnas
micro
time
Prior art date
Application number
AT05810351T
Other languages
English (en)
Inventor
Andrew Finn
Caifu Chen
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE494392T1 publication Critical patent/ATE494392T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05810351T 2004-09-21 2005-09-21 Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) ATE494392T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61219904P 2004-09-21 2004-09-21
PCT/US2005/033943 WO2006034387A1 (en) 2004-09-21 2005-09-21 TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)

Publications (1)

Publication Number Publication Date
ATE494392T1 true ATE494392T1 (de) 2011-01-15

Family

ID=35645577

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05810351T ATE494392T1 (de) 2004-09-21 2005-09-21 Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)

Country Status (6)

Country Link
US (3) US7642055B2 (de)
EP (2) EP1791982B1 (de)
JP (1) JP2008513028A (de)
AT (1) ATE494392T1 (de)
DE (1) DE602005025782D1 (de)
WO (1) WO2006034387A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
EP1791982B1 (de) 2004-09-21 2011-01-05 Life Technologies Corporation Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
WO2007117256A1 (en) * 2005-05-31 2007-10-18 Applera Corporation Multiplexed amplification of short nucleic acids
ES2448491T3 (es) 2006-03-02 2014-03-14 The Ohio State University Research Foundation Perfil de expresión de microARN asociado con cáncer de páncreas
US20080038727A1 (en) * 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
US7727745B2 (en) * 2006-08-14 2010-06-01 Bio-Id Diagnostic Inc. Synthesis of single-stranded DNA
US20080161197A1 (en) * 2006-12-12 2008-07-03 Kai Qin Lao Method for amplifying monomorphic-tailed nucleic acids
US20090023190A1 (en) 2007-06-20 2009-01-22 Kai Qin Lao Sequence amplification with loopable primers
CN101849008A (zh) 2007-09-19 2010-09-29 应用生物系统有限公司 用于减少RNAi中的脱靶表型效应的siRNA的不依赖于序列的修饰形式和其稳定形式
US20090291475A1 (en) 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
DE102012215925B3 (de) * 2012-09-07 2013-09-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Zeitgleicher Nachweis verschiedener microRNA-Biogenese-Formen
WO2017158840A1 (ja) 2016-03-18 2017-09-21 株式会社 東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
SG11202104409YA (en) 2018-10-31 2021-05-28 Zymergen Inc Multiplexed deterministic assembly of dna libraries

Family Cites Families (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US6197563B1 (en) * 1985-03-28 2001-03-06 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences
US6040166A (en) * 1985-03-28 2000-03-21 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences, including a probe
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
ES2128323T3 (es) 1990-07-24 1999-05-16 Hoffmann La Roche Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
WO1994013689A1 (en) * 1992-12-09 1994-06-23 Miller Jeffrey E METHODS AND COMPOSITIONS FOR cDNA SYNTHESIS
US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US5876927A (en) * 1993-07-13 1999-03-02 The Regents Of The University Of California Nucleic acid diagnostic assay for Charcot-Marie-Tooth Disease Type 1B
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6821727B1 (en) * 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
CA2159830C (en) 1994-04-29 2001-07-03 Applied Biosystems, Llc System for real time detection of nucleic acid amplification products
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
EP0832287B1 (de) 1995-06-07 2007-10-10 Solexa, Inc Oligonukleotid-tags zur sortierung und identifizierung
US6153425A (en) 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP2332957B1 (de) 1996-02-09 2015-04-08 Cornell Research Foundation, Inc. Detektion von Unterschieden in der Sequenz von Nukleinsäuren mittels der Ligasedetektionsreaktion und adressierbaren Arrays
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5982405A (en) 1996-04-18 1999-11-09 Japan Servo Co., Ltd. Thermal transfer recording apparatus and transfer ribbon
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5736333A (en) 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
CA2744096C (en) * 1996-07-31 2013-07-30 Laboratory Corporation Of America Holdings Biomarkers and targets for diagnosis, prognosis and management of prostate disease
WO1998015644A2 (en) 1996-09-27 1998-04-16 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method
US6090557A (en) * 1997-04-18 2000-07-18 Roche Molecular Systems, Inc. Neisseria gonorrhoeae-specific oligonucleotides
US6309829B1 (en) 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6355421B1 (en) 1997-10-27 2002-03-12 Boston Probes, Inc. Methods, kits and compositions pertaining to PNA molecular beacons
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6582936B1 (en) * 1997-12-12 2003-06-24 The Regents Of The University Of California Methods for making nucleic acids
US6114152A (en) * 1997-12-12 2000-09-05 The Regents Of The University Of California Methods for making nucleic acids
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
CA2318880C (en) * 1998-02-04 2010-07-27 Applied Biosystems, Llc Determination of a genotype of an amplification product at multiple allelic sites
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6451525B1 (en) 1998-12-03 2002-09-17 Pe Corporation (Ny) Parallel sequencing method
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6383752B1 (en) 1999-03-31 2002-05-07 Hybridon, Inc. Pseudo-cyclic oligonucleobases
JP2003510017A (ja) * 1999-06-22 2003-03-18 インビトロジェン コーポレイション 核酸の検出および識別のために改良されたプライマーおよび方法
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
JP2003527087A (ja) * 1999-08-13 2003-09-16 イェール・ユニバーシティ バイナリーコード化配列タグ
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
EP1313880A2 (de) 2000-05-30 2003-05-28 PE Corporation (NY) Methoden zur detektion gewünschter nukleinsäuren mittels kombinierter ligation und amplifizierung
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6358679B1 (en) * 2000-08-24 2002-03-19 Pe Corporation (Ny) Methods for external controls for nucleic acid amplification
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6777180B1 (en) * 2000-11-28 2004-08-17 Trustees Of Columbia University In The City Of New York Method for full-length cDNA cloning using degenerate stem-loop annealing primers
EP1360333A2 (de) 2001-01-31 2003-11-12 Ambion, Inc. Vergleichende auswertung der nukleinsäuren mit population tagging
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6589250B2 (en) 2001-11-20 2003-07-08 Stephen A. Schendel Maxillary distraction device
US20030235854A1 (en) * 2002-05-09 2003-12-25 Chan Eugene Y. Methods for analyzing a nucleic acid
WO2004022784A2 (en) * 2002-09-06 2004-03-18 Johns Hopkins Singapore Pte Ltd Strand specific detection and quantification
CN1318606C (zh) * 2002-09-19 2007-05-30 阿普里拉股份有限公司 检测靶序列的方法和组合物
AU2003265483A1 (en) * 2002-09-20 2004-04-08 Pharmacia & Upjohn Company Llc A METHOD FOR GENERATION OF A RANDOM RNAi LIBRARY AND ITS APPLICATION IN CELL-BASED SCREENS
US20040175732A1 (en) * 2002-11-15 2004-09-09 Rana Tariq M. Identification of micrornas and their targets
AU2003295745A1 (en) 2002-11-19 2004-06-15 Applera Corporation Polynucleotide sequence detection assays
WO2005007815A2 (en) * 2003-07-07 2005-01-27 One Cell Systems, Inc. Hairpin-labeled probes and methods of use
DE602004026338D1 (de) * 2003-11-04 2010-05-12 Applied Biosystems Llc Konkatamere ligationsprodukte: zusammensetzungen, verfahren und kits dafür
US7604937B2 (en) * 2004-03-24 2009-10-20 Applied Biosystems, Llc Encoding and decoding reactions for determining target polynucleotides
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
EP1791982B1 (de) 2004-09-21 2011-01-05 Life Technologies Corporation Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
WO2006071582A2 (en) * 2004-12-29 2006-07-06 Applera Corporation Methods, compositions, and kits for forming self-complementary polynucleotides
US20070015176A1 (en) * 2005-02-18 2007-01-18 Applera Corporation Small nucleic acid detection probes and uses thereof
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas
ATE532878T1 (de) * 2005-08-24 2011-11-15 Life Technologies Corp Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
FR2989691B1 (fr) 2012-04-24 2014-05-23 Commissariat Energie Atomique Reacteur pour le depot de couche atomique (ald), application a l'encapsulage d'un dispositif oled par depot de couche transparente en al2o3.
JP6102810B2 (ja) 2014-03-25 2017-03-29 富士ゼロックス株式会社 帯電部材、帯電装置、プロセスカートリッジ、画像形成装置、および帯電部材の製造方法

Also Published As

Publication number Publication date
WO2006034387A1 (en) 2006-03-30
EP1791982A1 (de) 2007-06-06
EP1791982B1 (de) 2011-01-05
US20060078924A1 (en) 2006-04-13
JP2008513028A (ja) 2008-05-01
US20100203545A1 (en) 2010-08-12
EP2186908A1 (de) 2010-05-19
US7642055B2 (en) 2010-01-05
US20090087858A1 (en) 2009-04-02
DE602005025782D1 (de) 2011-02-17

Similar Documents

Publication Publication Date Title
Srivastava et al. Isothermal nucleic acid amplification and its uses in modern diagnostic technologies
DE602005025782D1 (de) Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
ATE521717T1 (de) Parallele nukleinsäuresequenzierungsverfahren
IS8314A (is) Ákvörðun fjölkirnisraðar
WO2009074983A3 (en) Assay for detecting circulating free nucleic acids
ATE398187T1 (de) Verfahren zum nachweis von ziel-nukleinsäuren mittels pcr
EP1601780A4 (de) Erstellung eines echtzeit-genexpressionsprofils
WO2007100711A3 (en) Methods and compositions for detecting polynucleotides
DE50210199D1 (de) Verfahren zum nachweis von cytosinmethylierungsmustern mit hoher sensitivität
WO2004072297A3 (en) Terminal-phosphate-labeled nucleotides and methods of use
EA200500041A1 (ru) Способ обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров
ATE532878T1 (de) Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
WO2005047543A3 (en) Ligation assay
JP2012529904A5 (de)
ATE288964T1 (de) Methoden für externe kontrollen zur nukleinsäure amplifizierung
EP3303613A1 (de) Verfahren zur erkennung der aktivität eines polymerase
CN108753933B (zh) 一种基于金属钌配合物的实时荧光定量pcr检测方法及试剂盒
WO2004046344A3 (en) Polynucleotide sequence detection assays
RU2014102106A (ru) Композиции, способы и наборы для гибридизации нуклеиновой кислоты
GB2444101A (en) Methods and compositions for detecting polynucleotides
Pu et al. A real-time decoding sequencing based on dual mononucleotide addition for cyclic synthesis
WO2011114142A3 (en) Sequence detection assay
US7838236B2 (en) Analytical method and kit
EP1862558A4 (de) Gennachweisverfahren
ATE553221T1 (de) Verfahren zum nachweis von amplifikation oder deletion in einem genomischen dna-fragment

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties