ATE521717T1 - Parallele nukleinsäuresequenzierungsverfahren - Google Patents

Parallele nukleinsäuresequenzierungsverfahren

Info

Publication number
ATE521717T1
ATE521717T1 AT04768958T AT04768958T ATE521717T1 AT E521717 T1 ATE521717 T1 AT E521717T1 AT 04768958 T AT04768958 T AT 04768958T AT 04768958 T AT04768958 T AT 04768958T AT E521717 T1 ATE521717 T1 AT E521717T1
Authority
AT
Austria
Prior art keywords
sequencing
molecules
nucleic acid
acid sequencing
sequencing methods
Prior art date
Application number
AT04768958T
Other languages
English (en)
Inventor
Mikhail Shchepinov
Kalim Mir
Original Assignee
Isis Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation filed Critical Isis Innovation
Application granted granted Critical
Publication of ATE521717T1 publication Critical patent/ATE521717T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04768958T 2003-10-20 2004-10-20 Parallele nukleinsäuresequenzierungsverfahren ATE521717T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0324456.3A GB0324456D0 (en) 2003-10-20 2003-10-20 Parallel DNA sequencing methods
PCT/GB2004/004432 WO2005040425A2 (en) 2003-10-20 2004-10-20 Nucleic acid sequencing methods

Publications (1)

Publication Number Publication Date
ATE521717T1 true ATE521717T1 (de) 2011-09-15

Family

ID=29559567

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04768958T ATE521717T1 (de) 2003-10-20 2004-10-20 Parallele nukleinsäuresequenzierungsverfahren

Country Status (5)

Country Link
US (3) US20070219367A1 (de)
EP (4) EP2270205A3 (de)
AT (1) ATE521717T1 (de)
GB (1) GB0324456D0 (de)
WO (1) WO2005040425A2 (de)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103396933B (zh) 2003-02-26 2016-04-20 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US20080026380A1 (en) * 2006-07-31 2008-01-31 Siddiqi Suhaib M Nucleotide analogs
US7405434B2 (en) * 2004-11-16 2008-07-29 Cornell Research Foundation, Inc. Quantum dot conjugates in a sub-micrometer fluidic channel
WO2006064199A1 (en) * 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
EP2230316A1 (de) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
EP3257949A1 (de) 2005-06-15 2017-12-20 Complete Genomics Inc. Nukleinsäureanalyse durch zufällige mischungen von nichtüberlappenden fragmenten
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
CA2616433A1 (en) * 2005-07-28 2007-02-01 Helicos Biosciences Corporation Consecutive base single molecule sequencing
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
SG169356A1 (en) 2006-02-08 2011-03-30 Illumina Cambridge Ltd Method for sequencing a polynucleotide template
US20070202521A1 (en) * 2006-02-14 2007-08-30 Applera Corporation Single Molecule DNA Sequencing Using Fret Based Dynamic Labeling
US8301394B2 (en) 2006-02-16 2012-10-30 454 Life Sciences Corporation System and method for correcting primer extension errors in nucleic acid sequence data
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101495654A (zh) 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
EP3978121A1 (de) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanodüsen-geräteanordnungen: ihre herstellung und verwendung zur makromolekularen analyse
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US8315817B2 (en) * 2007-01-26 2012-11-20 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US12497656B2 (en) 2007-01-26 2025-12-16 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US20100035268A1 (en) * 2007-02-21 2010-02-11 Joseph Beechem Materials and methods for single molecule nucleic acid sequencing
EP2604344A3 (de) 2007-03-28 2014-07-16 BioNano Genomics, Inc. Verfahren zur makromolekularen Analyse mittels Nanokanalanordnungen
WO2008119181A1 (en) * 2007-04-02 2008-10-09 Mcmaster University Stabilized gold nanoparticles and methods of making the same
EP2171097A2 (de) * 2007-06-29 2010-04-07 Population Genetics Technologies LTD. Verfahren und zusammensetzungen zur isolierung der varianten von nukleinsäuresequenzen
JP2010532485A (ja) 2007-06-29 2010-10-07 アプライド バイオシステムズ ナノｆｅｔを含む電子検出のためのシステムおよび方法
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
EP2203568A1 (de) * 2007-10-22 2010-07-07 Life Technologies Corporation Verfahren und system zur gewinnung geordneter, segmentierter sequenzfragmente entlang eines nukleinsäuremoleküls
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8211673B2 (en) * 2008-02-04 2012-07-03 Life Technologies Corporation Composition and method for sequencing nucleic acid
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US8623598B2 (en) * 2008-03-19 2014-01-07 Intelligent Bio Systems, Inc. Methods and compositions for inhibiting undesired cleaving of labels
WO2010033267A2 (en) * 2008-05-06 2010-03-25 University Of Georgia Research Foundation, Inc. Structures, methods of making structures, multi-well array surface enhanced raman spectroscopy (sers) chips, methods of making, and methods of use
US8628919B2 (en) 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US10759824B2 (en) * 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
CN108467887A (zh) 2008-11-18 2018-08-31 生物纳米基因公司 多核苷酸作图和测序
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
PT2669387T (pt) 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
EP2491138A1 (de) * 2009-10-21 2012-08-29 Bionano Genomics, Inc. Verfahren und zugehörige vorrichtungen zur gesamtgenomanalyse eines einzelmoleküls
WO2011050340A1 (en) * 2009-10-23 2011-04-28 Life Technologies Corporation Systems and methods for error correction in dna sequencing
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
AU2011226792A1 (en) 2010-06-11 2012-01-12 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
ES2676183T3 (es) 2010-07-02 2018-07-17 Ventana Medical Systems, Inc. Detección de dianas usando marcas de masa y espectrometría de masas
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US8666678B2 (en) 2010-10-27 2014-03-04 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
EP2659408B1 (de) 2010-12-29 2019-03-27 Life Technologies Corporation Zeitverzerrtes hintergrundsignal für sequenzierung-mit-synthese-operationen
EP3582224B1 (de) 2010-12-30 2026-04-29 Life Technologies Corporation Modelle zur analyse von daten aus sequenzierung-mit-synthese-operationen
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
CN103764845B (zh) 2011-04-08 2016-02-17 生命科技股份有限公司 用于合成测序中的相保护试剂流排序
WO2012166647A1 (en) * 2011-05-27 2012-12-06 Life Technologies Corporation Methods for manipulating biomolecules
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2844774B1 (de) * 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna-sequenzierung
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10192024B2 (en) * 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
JPWO2014034275A1 (ja) * 2012-08-30 2016-08-08 株式会社日立ハイテクノロジーズ 核酸分析装置
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
US10428379B2 (en) 2013-03-15 2019-10-01 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
CN105683980B (zh) 2013-10-04 2018-08-24 生命科技股份有限公司 在使用终止化学物质的测序中建立分阶段效应模型的方法和系统
WO2015194663A1 (ja) * 2014-06-20 2015-12-23 コニカミノルタ株式会社 表面プラズモン共鳴励起増強蛍光分光（ｓｐｆｓ）用センサーチップ、ｓｐｆｓ測定法、およびｓｐｆｓ用キット
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN104237203B (zh) * 2014-09-28 2016-08-24 苏州大学 一种定量检测水样汞离子浓度的sers传感器及其制备方法
US10648103B2 (en) 2014-10-10 2020-05-12 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
CN107002126B (zh) * 2014-10-24 2021-05-25 昆塔波尔公司 使用纳米结构阵列的聚合物的高效光学分析
EP4220645A3 (de) 2015-05-14 2023-11-08 Life Technologies Corporation Barcodesequenzen sowie zugehörige systeme und verfahren
WO2016185284A1 (en) 2015-05-20 2016-11-24 Boreal Genomics, Inc. Method for isolating target nucleic acid using heteroduplex binding proteins
CN108779138B (zh) 2015-09-28 2022-06-17 哥伦比亚大学董事会 用作dna合成测序的可逆终止物的基于新的二硫键接头的核苷酸的设计与合成
CN105400781B (zh) * 2015-11-12 2018-08-24 广东省生态环境与土壤研究所 一种双嵌段分子探针及其快速检测核酸方法
EP3411494B1 (de) * 2015-11-18 2025-08-13 Kalim U. Mir Hochauflösende sequenzierung
CN116218970A (zh) * 2015-11-19 2023-06-06 赛纳生物科技(北京)有限公司 用于获得和校正目标多核苷酸的序列信息的方法
EP3400444A4 (de) 2016-01-04 2019-10-16 QuantuMDx Group Limited Entwurf, synthese und verwendung synthetischer nukleotide mit ladungsmassenmarkierungen
WO2017127759A1 (en) * 2016-01-22 2017-07-27 The Regents Of The University Of California Formation and use of embedded solutions in nanoscale materials
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017205336A1 (en) 2016-05-23 2017-11-30 The Trustees Of Columbia University In The City Of New York Nucleotide derivatives and methods of use thereof
EP4361287A3 (de) * 2016-09-28 2024-08-07 Life Technologies Corporation Verfahren zur sequenzierung von nukleinsäuren mittels terminierungschemie
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN108265112B (zh) 2016-12-30 2023-04-25 深圳市真迈生物科技有限公司 对序列测定反应进行控制的方法、序列测定系统和控制装置
EP3596099B1 (de) 2017-03-06 2024-07-24 Singular Genomics Systems, Inc. Verfahren zur nukleinsäuresequenzierung durch synthese (sbs), die sbs-zyklusschritte kombinieren
WO2018183538A1 (en) 2017-03-28 2018-10-04 The Trustees Of Columbia University In The City Of New York 3'-o-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for dna sequencing by synthesis
KR102709865B1 (ko) 2017-05-05 2024-09-26 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재
US11473090B2 (en) * 2017-05-16 2022-10-18 Helix Nanotechnologies, Inc. Linear covalently closed vectors and related compositions and methods thereof
WO2019023257A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
CN109957607A (zh) * 2017-12-14 2019-07-02 深圳先进技术研究院 一种检测探针及其应用
US12215124B2 (en) 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
CN112218640A (zh) 2018-03-15 2021-01-12 哥伦比亚大学董事会 核苷酸类似物及其在核酸测序和分析中的用途
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
CA3105715A1 (en) 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
EP3826762A1 (de) 2018-07-23 2021-06-02 DNA Script Massiv parallele enzymatische synthese von nukleinsäuresträngen
CN113039191B (zh) 2018-10-25 2024-07-16 奇异基因组学系统公司 核苷酸类似物
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
CN112867800B (zh) * 2018-12-17 2024-07-12 Illumina公司 用于制备测序文库的方法和手段
JP2022516684A (ja) 2019-01-08 2022-03-01 シンギュラー・ゲノミクス・システムズ・インコーポレイテッド ヌクレオチドの開裂可能なリンカーおよびその使用
WO2020154546A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
KR20260033107A (ko) 2019-02-19 2026-03-10 울티마 제노믹스, 인크. 광학 검출 및 시퀀싱을 위한 링커 및 방법
US11959105B2 (en) 2019-06-28 2024-04-16 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
WO2021041874A1 (en) 2019-08-29 2021-03-04 Martin David C Biofunctional thiophene monomers
US11712715B2 (en) 2019-10-11 2023-08-01 Quantum-Si Incorporated Surface modification in the vapor phase
IL292313A (en) * 2019-10-18 2022-06-01 Univ Leland Stanford Junior Clinical- and industrial-scale intact-tissue sequencing
US12011716B2 (en) 2019-10-29 2024-06-18 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
WO2021150614A1 (en) 2020-01-21 2021-07-29 Quantum-Si Incorporated Compounds and methods for selective c-terminal labeling
TWI724764B (zh) * 2020-01-21 2021-04-11 國立臺灣大學 光譜照相裝置
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
EP4143579A2 (de) 2020-05-20 2023-03-08 Quantum-si Incorporated Verfahren und zusammensetzungen zur proteinsequenzierung
CN114350774B (zh) * 2022-01-28 2024-07-09 赛纳生物科技(北京)有限公司 一种芯片表面固相引物剪切效率的检测方法
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging
WO2026013914A1 (ja) * 2024-07-12 2026-01-15 株式会社日立ハイテク 一塩基伸長反応による遺伝子検出方法及び手段

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
WO1996027025A1 (en) * 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
PL331513A1 (en) 1996-06-06 1999-07-19 Lynx Therapeutics Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters
JP2002503954A (ja) * 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
WO2000055372A1 (en) * 1999-03-16 2000-09-21 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
WO2000036152A1 (en) * 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001016375A2 (en) * 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
DE60027040T2 (de) * 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
IL150291A0 (en) * 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
WO2001068913A2 (en) * 2000-03-13 2001-09-20 Genset Nucleic acid detection method and system
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
ATE377093T1 (de) * 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
US6818907B2 (en) 2000-10-17 2004-11-16 The President And Fellows Of Harvard College Surface plasmon enhanced illumination system
DE60222628T2 (de) 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
EP2465943A3 (de) 2001-03-16 2012-10-03 Kalim Mir Lineare Polymeranzeige
US20030054396A1 (en) * 2001-09-07 2003-03-20 Weiner Michael P. Enzymatic light amplification
WO2003068986A1 (en) * 2002-02-11 2003-08-21 Applied Research Systems Ars Holding N.V. Nucleotide detection by fluorescence resonance energy transfer and methods thereof
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
EP1497304B1 (de) * 2002-04-12 2014-06-25 Catalyst Assets LLC Doppelt markierte nukleotide
EP1497303A2 (de) * 2002-04-22 2005-01-19 Joseph F. Lawler Jr. Verbindungen zur überwachung von nuklein säuere amplification und verfahren zu dessen verwendung
ATE432365T1 (de) * 2003-02-21 2009-06-15 Geneform Technologies Ltd Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
US20050112671A1 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. FRET efficiency methods

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US20140162892A1 (en) 2014-06-12
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