ATE521717T1 - Parallele nukleinsäuresequenzierungsverfahren - Google Patents

Parallele nukleinsäuresequenzierungsverfahren

Info

Publication number
ATE521717T1
ATE521717T1 AT04768958T AT04768958T ATE521717T1 AT E521717 T1 ATE521717 T1 AT E521717T1 AT 04768958 T AT04768958 T AT 04768958T AT 04768958 T AT04768958 T AT 04768958T AT E521717 T1 ATE521717 T1 AT E521717T1
Authority
AT
Austria
Prior art keywords
sequencing
molecules
nucleic acid
acid sequencing
sequencing methods
Prior art date
Application number
AT04768958T
Other languages
English (en)
Inventor
Mikhail Shchepinov
Kalim Mir
Original Assignee
Isis Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation filed Critical Isis Innovation
Application granted granted Critical
Publication of ATE521717T1 publication Critical patent/ATE521717T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04768958T 2003-10-20 2004-10-20 Parallele nukleinsäuresequenzierungsverfahren ATE521717T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0324456.3A GB0324456D0 (en) 2003-10-20 2003-10-20 Parallel DNA sequencing methods
PCT/GB2004/004432 WO2005040425A2 (en) 2003-10-20 2004-10-20 Nucleic acid sequencing methods

Publications (1)

Publication Number Publication Date
ATE521717T1 true ATE521717T1 (de) 2011-09-15

Family

ID=29559567

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04768958T ATE521717T1 (de) 2003-10-20 2004-10-20 Parallele nukleinsäuresequenzierungsverfahren

Country Status (5)

Country Link
US (3) US20070219367A1 (de)
EP (4) EP1689881B1 (de)
AT (1) ATE521717T1 (de)
GB (1) GB0324456D0 (de)
WO (1) WO2005040425A2 (de)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2897376A1 (en) 2003-02-26 2004-09-10 Radoje T. Drmanac Modular system and probes for dna analysis
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US20080026380A1 (en) * 2006-07-31 2008-01-31 Siddiqi Suhaib M Nucleotide analogs
US7405434B2 (en) * 2004-11-16 2008-07-29 Cornell Research Foundation, Inc. Quantum dot conjugates in a sub-micrometer fluidic channel
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
EP1828412B2 (de) * 2004-12-13 2019-01-09 Illumina Cambridge Limited Verbessertes nukleotidnachweisverfahren
EP2272983A1 (de) * 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US20070099212A1 (en) * 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing
US7666593B2 (en) * 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
US20070202521A1 (en) * 2006-02-14 2007-08-30 Applera Corporation Single Molecule DNA Sequencing Using Fret Based Dynamic Labeling
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
EP2002367B1 (de) * 2006-02-16 2017-03-29 454 Life Sciences Corporation System und verfahren zur korrektur von primerextensionsfehlern bei nukleinsäuresequenzdaten
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
JP2009538123A (ja) 2006-04-19 2009-11-05 アプライド バイオシステムズ, エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
EP2049262A2 (de) 2006-07-19 2009-04-22 Bionanomatrix, Inc. Nanodüsen-geräteanordnungen: ihre herstellung und verwendung zur makromolekularen analyse
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US8315817B2 (en) * 2007-01-26 2012-11-20 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US12497656B2 (en) 2007-01-26 2025-12-16 Illumina, Inc. Independently removable nucleic acid sequencing system and method
EP2126072A4 (de) * 2007-02-21 2011-07-13 Life Technologies Corp Materialien und verfahren zur einzelmolekülsequenzierung von nukleinsäuren
CA2964611C (en) 2007-03-28 2021-06-01 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
CA2682513A1 (en) * 2007-04-02 2008-10-09 Mcmaster University Stabilized gold nanoparticles and methods of making and using the same
EP2171097A2 (de) 2007-06-29 2010-04-07 Population Genetics Technologies LTD. Verfahren und zusammensetzungen zur isolierung der varianten von nukleinsäuresequenzen
EP2165353B1 (de) * 2007-06-29 2017-08-30 Applied Biosystems, LLC Systeme und verfahren zur elektronischen erkennung mit nanofets
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
JP2011505119A (ja) * 2007-10-22 2011-02-24 ライフ テクノロジーズ コーポレーション 核酸分子に沿って順序付けられ分断化された配列断片を得るための方法およびシステム
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009100080A1 (en) * 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US20100035253A1 (en) * 2008-03-19 2010-02-11 Intelligent Bio-Systems, Inc. Methods And Compositions For Incorporating Nucleotides
WO2010033267A2 (en) * 2008-05-06 2010-03-25 University Of Georgia Research Foundation, Inc. Structures, methods of making structures, multi-well array surface enhanced raman spectroscopy (sers) chips, methods of making, and methods of use
CA3062190C (en) 2008-06-30 2023-03-28 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US10759824B2 (en) * 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
AU2009316628B2 (en) 2008-11-18 2016-06-16 Bionano Genomics, Inc. Polynucleotide mapping and sequencing
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
ES2595055T3 (es) 2009-08-25 2016-12-27 Illumina, Inc. Métodos para seleccionar y amplificar polinucleótidos
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
EP2491138A1 (de) * 2009-10-21 2012-08-29 Bionano Genomics, Inc. Verfahren und zugehörige vorrichtungen zur gesamtgenomanalyse eines einzelmoleküls
US9234239B2 (en) 2009-10-23 2016-01-12 Life Technologies Corporation Systems and methods for error correction in DNA sequencing
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
EP2952590B1 (de) 2010-06-11 2017-07-26 Life Technologies Corporation Alternative nukleotidflüsse in verfahren zur sequenzierung durch synthese
WO2012003478A2 (en) 2010-07-02 2012-01-05 Ventana Medical Systems, Inc. Detecting targets using mass tags and mass spectrometry
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
EP3141614B1 (de) 2010-10-27 2018-11-28 Life Technologies Corporation Prädiktives modell zur verwendung für sequenzierung nach synthese
WO2012058647A1 (en) 2010-10-28 2012-05-03 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
US9594870B2 (en) 2010-12-29 2017-03-14 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP3582224B1 (de) 2010-12-30 2026-04-29 Life Technologies Corporation Modelle zur analyse von daten aus sequenzierung-mit-synthese-operationen
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
EP3366782B1 (de) 2011-04-08 2021-03-10 Life Technologies Corporation Phasenschützende reagenzflussordnungen für die verwendung zur sequenzierung durch synthese
CN103827318B (zh) * 2011-05-27 2018-05-08 生命技术公司 用于操作生物分子的方法
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
EP3623481B1 (de) 2011-09-23 2021-08-25 Illumina, Inc. Zusammensetzungen zur nukleinsäuresequenzierung
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP3438286B1 (de) * 2012-05-02 2020-10-28 Ibis Biosciences, Inc. Dna-sequenzierung
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10192024B2 (en) * 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
JPWO2014034275A1 (ja) * 2012-08-30 2016-08-08 株式会社日立ハイテクノロジーズ 核酸分析装置
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
US10428379B2 (en) 2013-03-15 2019-10-01 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
EP3053072B1 (de) 2013-10-04 2024-02-21 Life Technologies Corporation Verfahren und systeme zur modellierung von phaseneffekten in der sequenzierung mittels terminierungschemie
JP6500897B2 (ja) * 2014-06-20 2019-04-17 コニカミノルタ株式会社 表面プラズモン共鳴励起増強蛍光分光(spfs)用センサーチップ、spfs測定法、およびspfs用キット
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN104237203B (zh) * 2014-09-28 2016-08-24 苏州大学 一种定量检测水样汞离子浓度的sers传感器及其制备方法
US10648103B2 (en) 2014-10-10 2020-05-12 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
CA2964790C (en) * 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
EP4220645A3 (de) 2015-05-14 2023-11-08 Life Technologies Corporation Barcodesequenzen sowie zugehörige systeme und verfahren
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
US11085076B2 (en) 2015-09-28 2021-08-10 The Trustees Of Columbia University In The City Of New York Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis
CN105400781B (zh) * 2015-11-12 2018-08-24 广东省生态环境与土壤研究所 一种双嵌段分子探针及其快速检测核酸方法
WO2017087823A1 (en) * 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
WO2017084580A1 (en) * 2015-11-19 2017-05-26 Peking University Methods for obtaining and correcting biological sequence information
EP3400444A4 (de) 2016-01-04 2019-10-16 QuantuMDx Group Limited Entwurf, synthese und verwendung synthetischer nukleotide mit ladungsmassenmarkierungen
WO2017127759A1 (en) * 2016-01-22 2017-07-27 The Regents Of The University Of California Formation and use of embedded solutions in nanoscale materials
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
EP3463381A4 (de) 2016-05-23 2020-01-01 The Trustees of Columbia University in the City of New York Nukleotidderivate und verfahren zur verwendung davon
WO2018064311A2 (en) * 2016-09-28 2018-04-05 Life Technologies Corporation Methods and systems for reducing phasing errors when sequencing nucleic acids using termination chemistry
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN108265112B (zh) 2016-12-30 2023-04-25 深圳市真迈生物科技有限公司 对序列测定反应进行控制的方法、序列测定系统和控制装置
US11591647B2 (en) 2017-03-06 2023-02-28 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
CN110709089B (zh) 2017-03-28 2024-12-06 哥伦比亚大学董事会 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物
CA3059211A1 (en) 2017-05-05 2018-11-08 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
WO2018213460A1 (en) 2017-05-16 2018-11-22 Helix Nanotechnologies, Inc. Linear covalently closed vectors and related compositions and methods thereof
KR102626317B1 (ko) 2017-07-24 2024-01-18 퀀텀-에스아이 인코포레이티드 고강도 표지된 반응물 조성물 및 서열분석 방법
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
CN109957607A (zh) * 2017-12-14 2019-07-02 深圳先进技术研究院 一种检测探针及其应用
US12215124B2 (en) 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
WO2019178393A1 (en) 2018-03-15 2019-09-19 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis
US12359253B2 (en) 2018-04-09 2025-07-15 The Board Of Trustees Of The Leland Stanford Junior University Method of in situ gene sequencing
EP3820943A1 (de) 2018-07-13 2021-05-19 Quantum-si Incorporated Bikonjugierbare etiketten und verwendungsverfahren
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
CA3106410A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
WO2020086834A1 (en) 2018-10-25 2020-04-30 Singular Genomics Systems, Inc. Nucleotide analogues
TWI863941B (zh) 2018-11-15 2024-12-01 美商寬騰矽公司 用於蛋白質定序之方法及組合物
CA3115155A1 (en) * 2018-12-17 2020-06-25 Illumina, Inc. Methods and means for preparing a library for sequencing
CN113453692A (zh) 2019-01-08 2021-09-28 奇异基因组学系统公司 核苷酸可裂解接头和其用途
CN113614097A (zh) 2019-01-23 2021-11-05 宽腾矽公司 高强度标记的反应物组合物和测序方法
CN113710686A (zh) 2019-02-19 2021-11-26 阿尔缇玛基因组学公司 用于光学检测和测序的接头和方法
EP3976771A1 (de) 2019-06-28 2022-04-06 Quantum-si Incorporated Polymerisationsenzyme für sequenzierungsreaktionen
WO2021041874A1 (en) 2019-08-29 2021-03-04 Martin David C Biofunctional thiophene monomers
BR112022006682A2 (pt) 2019-10-11 2022-07-12 Quantum Si Inc Modificação de superfície na fase de vapor
US20230109070A1 (en) * 2019-10-18 2023-04-06 The Board of Trustees og the Leland Standford Junior University Clinical- and industrial-scale intact-tissue sequencing
WO2021086985A1 (en) 2019-10-29 2021-05-06 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
JP2023512634A (ja) 2020-01-21 2023-03-28 クアンタム-エスアイ インコーポレイテッド C末端の選択的標識のための化合物および方法
TWI724764B (zh) * 2020-01-21 2021-04-11 國立臺灣大學 光譜照相裝置
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
WO2021236983A2 (en) 2020-05-20 2021-11-25 Quantum-Si Incorporated Methods and compositions for protein sequencing
CN114350774B (zh) * 2022-01-28 2024-07-09 赛纳生物科技(北京)有限公司 一种芯片表面固相引物剪切效率的检测方法
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging
WO2026013914A1 (ja) * 2024-07-12 2026-01-15 株式会社日立ハイテク 一塩基伸長反応による遺伝子検出方法及び手段

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
AU5171696A (en) 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
HUP0003944A3 (en) 1996-06-06 2003-08-28 Lynx Therapeutics Inc Hayward Sequencing by ligation of encoded adaptors
EP1591541B1 (de) * 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
WO2000055372A1 (en) * 1999-03-16 2000-09-21 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
JP4638043B2 (ja) * 1998-12-14 2011-02-23 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU7086800A (en) * 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) * 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
DE60027040T2 (de) * 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
US20010039014A1 (en) * 2000-01-11 2001-11-08 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
AU4099001A (en) * 2000-03-13 2001-09-24 Genset Nucleic acid detection method and system
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
ATE377093T1 (de) * 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
US6818907B2 (en) 2000-10-17 2004-11-16 The President And Fellows Of Harvard College Surface plasmon enhanced illumination system
AU2002231933A1 (en) 2001-01-30 2002-08-12 Solexa Ltd. The preparation of polynucleotide arrays
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
DK1370690T3 (da) 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
US20030054396A1 (en) * 2001-09-07 2003-03-20 Weiner Michael P. Enzymatic light amplification
AU2003226829A1 (en) * 2002-02-11 2003-09-04 Applied Research Systems Ars Holding N.V. Nucleotide detection by fluorescence resonance energy transfer and methods thereof
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US20040014096A1 (en) * 2002-04-12 2004-01-22 Stratagene Dual-labeled nucleotides
EP1497303A2 (de) * 2002-04-22 2005-01-19 Joseph F. Lawler Jr. Verbindungen zur überwachung von nuklein säuere amplification und verfahren zu dessen verwendung
EP1594987B1 (de) * 2003-02-21 2009-05-27 GeneForm Technologies Limited Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
WO2005047471A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Improved fret efficiency methods

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US20140162892A1 (en) 2014-06-12
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WO2005040425A3 (en) 2005-07-28
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