ATE532878T1 - Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas - Google Patents
Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnasInfo
- Publication number
- ATE532878T1 ATE532878T1 AT06802531T AT06802531T ATE532878T1 AT E532878 T1 ATE532878 T1 AT E532878T1 AT 06802531 T AT06802531 T AT 06802531T AT 06802531 T AT06802531 T AT 06802531T AT E532878 T1 ATE532878 T1 AT E532878T1
- Authority
- AT
- Austria
- Prior art keywords
- mirnas
- quantifying
- dna
- polymorphoic
- sirnas
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108091070501 miRNA Proteins 0.000 title 2
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 102000012410 DNA Ligases Human genes 0.000 abstract 1
- 108010061982 DNA Ligases Proteins 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000010839 reverse transcription Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71148005P | 2005-08-24 | 2005-08-24 | |
| US75030205P | 2005-12-13 | 2005-12-13 | |
| US78331106P | 2006-03-16 | 2006-03-16 | |
| PCT/US2006/033642 WO2007025281A2 (en) | 2005-08-24 | 2006-08-24 | A method to quantify sirnas, mirnas and polymorphic mirnas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE532878T1 true ATE532878T1 (de) | 2011-11-15 |
Family
ID=37772526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06802531T ATE532878T1 (de) | 2005-08-24 | 2006-08-24 | Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas |
Country Status (4)
| Country | Link |
|---|---|
| US (4) | US20070111226A1 (de) |
| EP (1) | EP1924713B1 (de) |
| AT (1) | ATE532878T1 (de) |
| WO (1) | WO2007025281A2 (de) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| US7642055B2 (en) * | 2004-09-21 | 2010-01-05 | Applied Biosystems, Llc | Two-color real-time/end-point quantitation of microRNAs (miRNAs) |
| EP2195428B1 (de) | 2007-09-19 | 2013-12-11 | Applied Biosystems, LLC | Sirna-sequenz-unabhängige modifikationsformate zur verringerung von das ziel verfehlenden phänotypischen effekten bei rnai und stabilisierte formen davon |
| US20090181390A1 (en) * | 2008-01-11 | 2009-07-16 | Signosis, Inc. A California Corporation | High throughput detection of micrornas and use for disease diagnosis |
| KR101463874B1 (ko) * | 2008-01-24 | 2014-12-05 | 삼성전자 주식회사 | 검출용 올리고머 및 이를 이용한 바이오 칩의 ｑｃ 방법 |
| ITBO20080103A1 (it) * | 2008-02-15 | 2009-08-16 | Twof Inc | Microarray per l'analisi di sequenze nucleotidiche |
| WO2009136693A1 (en) * | 2008-05-06 | 2009-11-12 | Panagene Inc. | Peptide nucleic acid probes, kits and methods for expression profiling of micrornas |
| CA2749841A1 (en) | 2009-01-16 | 2010-07-22 | Cepheid | Methods of detecting cervical cancer |
| DK2391736T3 (en) | 2009-02-02 | 2015-05-11 | Exiqon As | A method for the quantitation of small RNA species |
| US9493832B2 (en) | 2009-02-02 | 2016-11-15 | Cepheid | Methods of detecting sepsis |
| EP2401406A4 (de) | 2009-02-25 | 2012-09-19 | Cepheid | Verfahren für den nachweis von lungenkrebs |
| EP2414541B8 (de) * | 2009-03-31 | 2014-09-10 | Centre Hospitalier Universitaire Vaudois | Methylierungs-/ligationsabhängiger makroarray (mlm) |
| GB0910302D0 (en) | 2009-06-15 | 2009-07-29 | Lumora Ltd | Nucleic acid amplification |
| EP2642291B1 (de) | 2009-08-07 | 2015-10-07 | Ohmx Corporation | Enzym-gesteuerter Redox-ändernder chemischer Eliminierungs- (E-trace)- Immunassay |
| US9250234B2 (en) | 2011-01-19 | 2016-02-02 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (E-TRACE) immunoassay |
| US20110117559A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| JP2013528379A (ja) | 2010-05-21 | 2013-07-11 | オームクス コーポレーション | Ｐｓａ酵素活性アッセイによる癌の検出 |
| US9850527B2 (en) * | 2010-06-14 | 2017-12-26 | National University Of Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR |
| JP6276706B2 (ja) | 2012-01-09 | 2018-02-07 | オームクス コーポレイション | Ｅ−ｔｒａｃｅアッセイシグナル増幅の酵素カスケード方法 |
| WO2013153911A1 (ja) * | 2012-04-12 | 2013-10-17 | 国立大学法人東京大学 | 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法 |
| CN103509789B (zh) * | 2012-06-26 | 2015-12-16 | 舟山医院 | 一种用于扩增短链rna的引物及其相关方法 |
| DK2914741T3 (da) * | 2012-11-02 | 2017-11-20 | Life Technologies Corp | Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet |
| WO2014110272A1 (en) | 2013-01-09 | 2014-07-17 | The Penn State Research Foundation | Low sequence bias single-stranded dna ligation |
| EP2971084A1 (de) | 2013-03-12 | 2016-01-20 | Cepheid | Verfahren zur krebserkennung |
| WO2014159073A1 (en) | 2013-03-14 | 2014-10-02 | Cepheid | Methods of detecting lung cancer |
| EP3047274A2 (de) | 2013-09-20 | 2016-07-27 | Ohmx Corporation | Psa-enzymaktivität: neuer biomarker zur bestimmung der aggressivität von prostatakrebs |
| WO2016025539A1 (en) | 2014-08-11 | 2016-02-18 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (-trace) assay with multiplexing capabilities |
| EP3967768A1 (de) | 2015-03-13 | 2022-03-16 | Life Technologies Corporation | Zusammensetzungen für erfassung, nachweis und quantifizierung von kleinen rna-molekülen |
| WO2016164866A1 (en) * | 2015-04-10 | 2016-10-13 | Hudsonalpha Institute For Biotechnology | METHOD FOR BLOCKING miRNA |
| JP6577660B2 (ja) | 2016-03-18 | 2019-09-18 | 株式会社東芝 | 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法 |
| US20190284621A1 (en) * | 2016-11-11 | 2019-09-19 | Roche Innovation Center Copenhagen A/S | Therapeutic oligonucleotides capture and detection |
| CN112105745A (zh) | 2018-05-07 | 2020-12-18 | 罗氏创新中心哥本哈根有限公司 | 用于寡核苷酸治疗剂的大规模平行发现方法 |
| US20230167487A2 (en) | 2021-07-12 | 2023-06-01 | Labsimply, Inc. | Crispr cascade assay |
| CN114085893B (zh) * | 2021-11-16 | 2023-07-25 | 圣湘生物科技股份有限公司 | 一种miRNA检测的引物探针设计方法、检测组合物及试剂盒 |
| US20230279375A1 (en) | 2021-12-13 | 2023-09-07 | Labsimply, Inc. | Signal boost cascade assay |
| WO2023114052A1 (en) * | 2021-12-13 | 2023-06-22 | Labsimply, Inc. | Tuning cascade assay kinetics via molecular design |
| EP4567129A3 (de) * | 2022-02-02 | 2025-08-13 | Guardant Health, Inc. | Multifunktionelle primer für gepaarte sequenzierungsablesungen |
| WO2024073114A1 (en) | 2022-09-30 | 2024-04-04 | Vedabio, Inc. | Delivery of therapeutics in vivo via a crispr-based cascade system |
| US11982677B2 (en) | 2022-10-02 | 2024-05-14 | Vedabio, Inc. | Dimerization screening assays |
| US11965205B1 (en) | 2022-10-14 | 2024-04-23 | Vedabio, Inc. | Detection of nucleic acid and non-nucleic acid target molecules |
| WO2024253707A2 (en) | 2023-01-07 | 2024-12-12 | Vedabio, Inc. | Engineered nucleic acid-guided nucleases |
| US12060602B2 (en) | 2023-01-10 | 2024-08-13 | Vedabio, Inc. | Sample splitting for multiplexed detection of nucleic acids without amplification |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6027923A (en) * | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
| US6072923A (en) * | 1996-04-30 | 2000-06-06 | Wavefront Research, Inc. | Optical switching, routing, and time delay systems using switched mirrors |
| EP2369007B1 (de) * | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Erkennung von Nukleinsäuresequenz-Unterschieden mittels der gleichzeitigen Ligaseerkennungs- und Polymerasekettenreaktion |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5968800A (en) * | 1997-02-07 | 1999-10-19 | Merck & Co., Inc. | Cyclin-dependent protein kinase |
| JP2001515362A (ja) | 1997-05-13 | 2001-09-18 | ディスプレイ・システムズ・バイオテック・アクティーゼルスカブ | ｍＲＮＡをクローニングする方法および差次的に発現された転写物のディスプレイ（ＤＯＤＥＴ） |
| US6406891B1 (en) * | 1998-09-28 | 2002-06-18 | Board Of Regents, The University Of Texas System | Dual RT procedure for cDNA synthesis |
| US6177635B1 (en) * | 1999-01-12 | 2001-01-23 | Yazaki Corporation | Wiring substrate and manufacturing method thereof |
| WO2000050611A1 (en) * | 1999-02-24 | 2000-08-31 | Sumitomo Electric Industries, Ltd. | Ganp proteins |
| US6528254B1 (en) * | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US6777180B1 (en) * | 2000-11-28 | 2004-08-17 | Trustees Of Columbia University In The City Of New York | Method for full-length cDNA cloning using degenerate stem-loop annealing primers |
| US7074558B2 (en) * | 2001-06-07 | 2006-07-11 | Pbi Technology, Inc. | Nucleic acid amplification using an RNA polymerase and DNA/RNA mixed polymer intermediate products |
| US20040014058A1 (en) * | 2001-10-05 | 2004-01-22 | Alsobrook John P. | Novel human proteins, polynucleotides encoding them and methods of using the same |
| AU2003295600A1 (en) * | 2002-11-14 | 2004-06-15 | Dharmacon, Inc. | Functional and hyperfunctional sirna |
| US7851150B2 (en) * | 2002-12-18 | 2010-12-14 | Third Wave Technologies, Inc. | Detection of small nucleic acids |
| JP2007530051A (ja) * | 2004-03-24 | 2007-11-01 | アプレラ コーポレイション | 標的分子を決定するためのライゲーション反応および増幅反応 |
| US8192937B2 (en) * | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| US20060057595A1 (en) * | 2004-09-16 | 2006-03-16 | Applera Corporation | Compositions, methods, and kits for identifying and quantitating small RNA molecules |
-
2006
- 2006-08-24 EP EP06802531A patent/EP1924713B1/de active Active
- 2006-08-24 AT AT06802531T patent/ATE532878T1/de active
- 2006-08-24 US US11/467,125 patent/US20070111226A1/en not_active Abandoned
- 2006-08-24 WO PCT/US2006/033642 patent/WO2007025281A2/en not_active Ceased
-
2011
- 2011-01-27 US US13/015,332 patent/US8187815B2/en active Active
-
2012
- 2012-04-26 US US13/456,835 patent/US8710208B2/en active Active
-
2014
- 2014-03-28 US US14/229,706 patent/US20140377748A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8187815B2 (en) | 2012-05-29 |
| US20120301876A1 (en) | 2012-11-29 |
| US20140377748A1 (en) | 2014-12-25 |
| WO2007025281A2 (en) | 2007-03-01 |
| EP1924713A4 (de) | 2009-01-14 |
| EP1924713B1 (de) | 2011-11-09 |
| WO2007025281A3 (en) | 2007-11-01 |
| EP1924713A2 (de) | 2008-05-28 |
| US8710208B2 (en) | 2014-04-29 |
| US20110212494A1 (en) | 2011-09-01 |
| US20070111226A1 (en) | 2007-05-17 |
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