ATE495191T1 - Verfahren zur renaturierung rekombinanter, disulfid-enthaltender proteine bei hohen proteinkonzentrationen in gegenwart von aminen - Google Patents
Verfahren zur renaturierung rekombinanter, disulfid-enthaltender proteine bei hohen proteinkonzentrationen in gegenwart von aminenInfo
- Publication number
- ATE495191T1 ATE495191T1 AT02774777T AT02774777T ATE495191T1 AT E495191 T1 ATE495191 T1 AT E495191T1 AT 02774777 T AT02774777 T AT 02774777T AT 02774777 T AT02774777 T AT 02774777T AT E495191 T1 ATE495191 T1 AT E495191T1
- Authority
- AT
- Austria
- Prior art keywords
- disulfide
- amines
- high protein
- protein concentrations
- containing proteins
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 150000001412 amines Chemical class 0.000 title 1
- 108010039627 Aprotinin Proteins 0.000 abstract 2
- 102000004388 Interleukin-4 Human genes 0.000 abstract 1
- 108090000978 Interleukin-4 Proteins 0.000 abstract 1
- 230000003196 chaotropic effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
- 229940028885 interleukin-4 Drugs 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- 238000004153 renaturation Methods 0.000 abstract 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 abstract 1
- 150000003512 tertiary amines Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5406—IL-4
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01127373A EP1314739A1 (de) | 2001-11-22 | 2001-11-22 | Verfahren zur Renaturierung rekombinanter, Disulfid-enthaltender Proteine bei hohen Proteinkonzentrationen in Gegenwart von Aminen |
| PCT/EP2002/012607 WO2003044055A1 (en) | 2001-11-22 | 2002-11-12 | Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE495191T1 true ATE495191T1 (de) | 2011-01-15 |
Family
ID=8179272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02774777T ATE495191T1 (de) | 2001-11-22 | 2002-11-12 | Verfahren zur renaturierung rekombinanter, disulfid-enthaltender proteine bei hohen proteinkonzentrationen in gegenwart von aminen |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7893210B2 (de) |
| EP (2) | EP1314739A1 (de) |
| JP (1) | JP4348188B2 (de) |
| KR (1) | KR100944845B1 (de) |
| AT (1) | ATE495191T1 (de) |
| AU (1) | AU2002340510A1 (de) |
| CY (1) | CY1112209T1 (de) |
| DE (1) | DE60238938D1 (de) |
| DK (1) | DK1453858T3 (de) |
| ES (1) | ES2357888T3 (de) |
| PT (1) | PT1453858E (de) |
| WO (1) | WO2003044055A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7442370B2 (en) | 2001-02-01 | 2008-10-28 | Biogen Idec Ma Inc. | Polymer conjugates of mutated neublastin |
| CN101043899B (zh) | 2004-08-19 | 2011-03-30 | 比奥根艾迪克Ma公司 | 重折叠转化型生长因子beta家族蛋白 |
| TWI501774B (zh) | 2006-02-27 | 2015-10-01 | Biogen Idec Inc | 神經性病症之治療 |
| JP4984983B2 (ja) * | 2007-03-09 | 2012-07-25 | 富士通株式会社 | 符号化装置および符号化方法 |
| TWI445544B (zh) | 2007-05-01 | 2014-07-21 | Biogen Idec Inc | 增進血管形成之組合物及方法 |
| US10947521B2 (en) * | 2014-11-18 | 2021-03-16 | Merck Sharp & Dohme Corp. | Process for producing recombinant trypsin |
| CN113322222A (zh) * | 2021-07-02 | 2021-08-31 | 江苏恒丰强生物技术有限公司 | 一种表达猫ω干扰素的基因工程菌的生产工艺 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5453363A (en) * | 1985-10-23 | 1995-09-26 | Boehringer Mannheim Gmbh | Process for the activation of t-PA or Ing after genetic expression in prokaryotes |
| EP0301835A1 (de) * | 1987-07-29 | 1989-02-01 | Schering Biotech Corporation | Reinigung von in Escherichia coli exprimiertem, menschlichem Interleukin-4 |
| AU3350893A (en) * | 1991-07-29 | 1993-03-02 | Immunex Corporation | Process for isolating and purifying recombinant interleukin-7 |
| DE4137333A1 (de) | 1991-11-13 | 1993-05-19 | W Prof Dr Sebald | Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung |
| US5663304A (en) * | 1993-08-20 | 1997-09-02 | Genentech, Inc. | Refolding of misfolded insulin-like growth factor-I |
| US5407810A (en) * | 1993-08-20 | 1995-04-18 | Genentech, Inc. | Aqueous multiple-phase isolation of polypeptide |
| ATE189004T1 (de) * | 1994-10-13 | 2000-02-15 | Kyowa Medex Co Ltd | Verfahren zur bestimmung von harnstoff stickstoff |
| US5563057A (en) | 1994-10-31 | 1996-10-08 | Wisconsin Alumni Research Foundation | Method for refolding misfolded enzymes with detergent and cyclodextrin |
| US5728804A (en) | 1995-06-02 | 1998-03-17 | Research Corporation Technologies, Inc. | Use of cyclodextrins for protein renaturation |
| CA2223745C (en) * | 1995-06-07 | 2004-03-16 | Chiron Corporation | Method of solubilizing, purifying, and refolding protein |
| AU5195098A (en) * | 1996-11-06 | 1998-05-29 | Schering Corporation | Renaturation and purification of viral interleukin-10 (vil-10) |
| US20020052026A1 (en) * | 1997-10-08 | 2002-05-02 | Steven M. Vicik | Methods of refolding proteins |
| KR100253916B1 (ko) * | 1997-12-29 | 2000-05-01 | 김충환 | 사람 인슐린 전구체의 제조방법 |
| DE69929638T2 (de) | 1999-01-20 | 2006-09-21 | Bayer Healthcare Ag | Plasmide, deren Konstruktion und Anwendung zur Herstellung von Interleukin-4 und dessen Muteine |
| GB9912350D0 (en) | 1999-05-26 | 1999-07-28 | European Molecular Biology Lab Embl | Modified cytokine |
| NZ522847A (en) * | 2000-05-16 | 2004-11-26 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
-
2001
- 2001-11-22 EP EP01127373A patent/EP1314739A1/de not_active Withdrawn
-
2002
- 2002-11-12 KR KR1020047007769A patent/KR100944845B1/ko not_active Expired - Fee Related
- 2002-11-12 ES ES02774777T patent/ES2357888T3/es not_active Expired - Lifetime
- 2002-11-12 EP EP02774777A patent/EP1453858B1/de not_active Expired - Lifetime
- 2002-11-12 JP JP2003545690A patent/JP4348188B2/ja not_active Expired - Fee Related
- 2002-11-12 US US10/495,417 patent/US7893210B2/en not_active Expired - Fee Related
- 2002-11-12 WO PCT/EP2002/012607 patent/WO2003044055A1/en not_active Ceased
- 2002-11-12 AU AU2002340510A patent/AU2002340510A1/en not_active Abandoned
- 2002-11-12 DE DE60238938T patent/DE60238938D1/de not_active Expired - Lifetime
- 2002-11-12 PT PT02774777T patent/PT1453858E/pt unknown
- 2002-11-12 DK DK02774777.3T patent/DK1453858T3/da active
- 2002-11-12 AT AT02774777T patent/ATE495191T1/de active
-
2011
- 2011-03-29 CY CY20111100332T patent/CY1112209T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK1453858T3 (da) | 2011-05-09 |
| PT1453858E (pt) | 2011-03-16 |
| JP2005510217A (ja) | 2005-04-21 |
| US7893210B2 (en) | 2011-02-22 |
| EP1453858B1 (de) | 2011-01-12 |
| EP1453858A1 (de) | 2004-09-08 |
| KR100944845B1 (ko) | 2010-03-04 |
| ES2357888T3 (es) | 2011-05-03 |
| AU2002340510A1 (en) | 2003-06-10 |
| US20050014933A1 (en) | 2005-01-20 |
| CY1112209T1 (el) | 2015-12-09 |
| JP4348188B2 (ja) | 2009-10-21 |
| WO2003044055A1 (en) | 2003-05-30 |
| EP1314739A1 (de) | 2003-05-28 |
| KR20050058243A (ko) | 2005-06-16 |
| DE60238938D1 (de) | 2011-02-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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