DK1453858T3 - Fremgangsmåde til renaturering af rekombinante, disulfid-holdige proteiner ved høje proteinkoncentrationer i nærvær af aminer - Google Patents
Fremgangsmåde til renaturering af rekombinante, disulfid-holdige proteiner ved høje proteinkoncentrationer i nærvær af aminerInfo
- Publication number
- DK1453858T3 DK1453858T3 DK02774777.3T DK02774777T DK1453858T3 DK 1453858 T3 DK1453858 T3 DK 1453858T3 DK 02774777 T DK02774777 T DK 02774777T DK 1453858 T3 DK1453858 T3 DK 1453858T3
- Authority
- DK
- Denmark
- Prior art keywords
- renaturation
- amines
- high protein
- protein concentrations
- containing proteins
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 238000004153 renaturation Methods 0.000 title abstract 2
- 150000001412 amines Chemical class 0.000 title 1
- 108010039627 Aprotinin Proteins 0.000 abstract 2
- 102000004388 Interleukin-4 Human genes 0.000 abstract 1
- 108090000978 Interleukin-4 Proteins 0.000 abstract 1
- 230000003196 chaotropic effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
- 229940028885 interleukin-4 Drugs 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 abstract 1
- 150000003512 tertiary amines Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5406—IL-4
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01127373A EP1314739A1 (en) | 2001-11-22 | 2001-11-22 | Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines |
| PCT/EP2002/012607 WO2003044055A1 (en) | 2001-11-22 | 2002-11-12 | Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1453858T3 true DK1453858T3 (da) | 2011-05-09 |
Family
ID=8179272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK02774777.3T DK1453858T3 (da) | 2001-11-22 | 2002-11-12 | Fremgangsmåde til renaturering af rekombinante, disulfid-holdige proteiner ved høje proteinkoncentrationer i nærvær af aminer |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7893210B2 (da) |
| EP (2) | EP1314739A1 (da) |
| JP (1) | JP4348188B2 (da) |
| KR (1) | KR100944845B1 (da) |
| AT (1) | ATE495191T1 (da) |
| AU (1) | AU2002340510A1 (da) |
| CY (1) | CY1112209T1 (da) |
| DE (1) | DE60238938D1 (da) |
| DK (1) | DK1453858T3 (da) |
| ES (1) | ES2357888T3 (da) |
| PT (1) | PT1453858E (da) |
| WO (1) | WO2003044055A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7442370B2 (en) | 2001-02-01 | 2008-10-28 | Biogen Idec Ma Inc. | Polymer conjugates of mutated neublastin |
| KR101241551B1 (ko) | 2004-08-19 | 2013-03-11 | 바이오겐 아이덱 엠에이 인코포레이티드 | 전환 성장 인자 베타 패밀리 단백질의 리폴딩 |
| TWI501774B (zh) | 2006-02-27 | 2015-10-01 | Biogen Idec Inc | 神經性病症之治療 |
| JP4984983B2 (ja) * | 2007-03-09 | 2012-07-25 | 富士通株式会社 | 符号化装置および符号化方法 |
| ES2476253T3 (es) | 2007-05-01 | 2014-07-14 | Biogen Idec Ma Inc. | P�ptidos de neublastina para su uso en el aumento de la vascularizaci�n en tejido con flujo sanguíneo deteriorado |
| US10947521B2 (en) * | 2014-11-18 | 2021-03-16 | Merck Sharp & Dohme Corp. | Process for producing recombinant trypsin |
| CN113322222A (zh) * | 2021-07-02 | 2021-08-31 | 江苏恒丰强生物技术有限公司 | 一种表达猫ω干扰素的基因工程菌的生产工艺 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5453363A (en) * | 1985-10-23 | 1995-09-26 | Boehringer Mannheim Gmbh | Process for the activation of t-PA or Ing after genetic expression in prokaryotes |
| WO1989001046A1 (en) * | 1987-07-29 | 1989-02-09 | Schering Biotech Corporation | Purification of human interleukin-4 expressed in escherichia coli |
| AU3350893A (en) * | 1991-07-29 | 1993-03-02 | Immunex Corporation | Process for isolating and purifying recombinant interleukin-7 |
| DE4137333A1 (de) * | 1991-11-13 | 1993-05-19 | W Prof Dr Sebald | Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung |
| US5663304A (en) * | 1993-08-20 | 1997-09-02 | Genentech, Inc. | Refolding of misfolded insulin-like growth factor-I |
| US5407810A (en) * | 1993-08-20 | 1995-04-18 | Genentech, Inc. | Aqueous multiple-phase isolation of polypeptide |
| ATE189004T1 (de) * | 1994-10-13 | 2000-02-15 | Kyowa Medex Co Ltd | Verfahren zur bestimmung von harnstoff stickstoff |
| US5563057A (en) * | 1994-10-31 | 1996-10-08 | Wisconsin Alumni Research Foundation | Method for refolding misfolded enzymes with detergent and cyclodextrin |
| US5728804A (en) * | 1995-06-02 | 1998-03-17 | Research Corporation Technologies, Inc. | Use of cyclodextrins for protein renaturation |
| EP0837883B1 (en) * | 1995-06-07 | 2005-08-10 | Chiron Corporation | Method of solubilizing, purifying, and refolding protein |
| AU5195098A (en) * | 1996-11-06 | 1998-05-29 | Schering Corporation | Renaturation and purification of viral interleukin-10 (vil-10) |
| US20020052026A1 (en) * | 1997-10-08 | 2002-05-02 | Steven M. Vicik | Methods of refolding proteins |
| KR100253916B1 (ko) * | 1997-12-29 | 2000-05-01 | 김충환 | 사람 인슐린 전구체의 제조방법 |
| EP1022339B1 (en) | 1999-01-20 | 2006-02-01 | Bayer HealthCare AG | Plasmids, their construction and their use in the manufacture of interleukin-4 and interleukin-4 muteins |
| GB9912350D0 (en) | 1999-05-26 | 1999-07-28 | European Molecular Biology Lab Embl | Modified cytokine |
| IL152804A0 (en) * | 2000-05-16 | 2003-06-24 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
-
2001
- 2001-11-22 EP EP01127373A patent/EP1314739A1/en not_active Withdrawn
-
2002
- 2002-11-12 US US10/495,417 patent/US7893210B2/en not_active Expired - Fee Related
- 2002-11-12 EP EP02774777A patent/EP1453858B1/en not_active Expired - Lifetime
- 2002-11-12 PT PT02774777T patent/PT1453858E/pt unknown
- 2002-11-12 DK DK02774777.3T patent/DK1453858T3/da active
- 2002-11-12 WO PCT/EP2002/012607 patent/WO2003044055A1/en not_active Ceased
- 2002-11-12 AT AT02774777T patent/ATE495191T1/de active
- 2002-11-12 DE DE60238938T patent/DE60238938D1/de not_active Expired - Lifetime
- 2002-11-12 KR KR1020047007769A patent/KR100944845B1/ko not_active Expired - Fee Related
- 2002-11-12 JP JP2003545690A patent/JP4348188B2/ja not_active Expired - Fee Related
- 2002-11-12 ES ES02774777T patent/ES2357888T3/es not_active Expired - Lifetime
- 2002-11-12 AU AU2002340510A patent/AU2002340510A1/en not_active Abandoned
-
2011
- 2011-03-29 CY CY20111100332T patent/CY1112209T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20050014933A1 (en) | 2005-01-20 |
| EP1453858A1 (en) | 2004-09-08 |
| EP1314739A1 (en) | 2003-05-28 |
| JP2005510217A (ja) | 2005-04-21 |
| EP1453858B1 (en) | 2011-01-12 |
| JP4348188B2 (ja) | 2009-10-21 |
| KR20050058243A (ko) | 2005-06-16 |
| WO2003044055A1 (en) | 2003-05-30 |
| DE60238938D1 (de) | 2011-02-24 |
| CY1112209T1 (el) | 2015-12-09 |
| KR100944845B1 (ko) | 2010-03-04 |
| ATE495191T1 (de) | 2011-01-15 |
| PT1453858E (pt) | 2011-03-16 |
| ES2357888T3 (es) | 2011-05-03 |
| US7893210B2 (en) | 2011-02-22 |
| AU2002340510A1 (en) | 2003-06-10 |
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