ATE496298T1 - Methode um die sensitivität einer luminesenz assay zu erhöhen - Google Patents

Methode um die sensitivität einer luminesenz assay zu erhöhen

Info

Publication number
ATE496298T1
ATE496298T1 AT01942027T AT01942027T ATE496298T1 AT E496298 T1 ATE496298 T1 AT E496298T1 AT 01942027 T AT01942027 T AT 01942027T AT 01942027 T AT01942027 T AT 01942027T AT E496298 T1 ATE496298 T1 AT E496298T1
Authority
AT
Austria
Prior art keywords
fold
reduces
sensitivity
luminescence
assay
Prior art date
Application number
AT01942027T
Other languages
English (en)
Inventor
Erika Hawkins
John Centanni
Jacqueline Sankbeil
Keith Wood
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24364121&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE496298(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE496298T1 publication Critical patent/ATE496298T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Plural Heterocyclic Compounds (AREA)
AT01942027T 2000-06-09 2001-06-07 Methode um die sensitivität einer luminesenz assay zu erhöhen ATE496298T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/590,884 US7118878B1 (en) 2000-06-09 2000-06-09 Method for increasing luminescence assay sensitivity
PCT/US2001/018363 WO2001096862A2 (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity

Publications (1)

Publication Number Publication Date
ATE496298T1 true ATE496298T1 (de) 2011-02-15

Family

ID=24364121

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01942027T ATE496298T1 (de) 2000-06-09 2001-06-07 Methode um die sensitivität einer luminesenz assay zu erhöhen

Country Status (8)

Country Link
US (3) US7118878B1 (de)
EP (1) EP1297337B1 (de)
JP (2) JP4685325B2 (de)
AT (1) ATE496298T1 (de)
AU (2) AU2001275325B2 (de)
CA (1) CA2411179A1 (de)
DE (1) DE60143888D1 (de)
WO (1) WO2001096862A2 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
EP1546162B1 (de) 2002-09-20 2011-06-22 Promega Corporation Auf lumineszenz basierende verfahren und sonden zur messung der cytochrom-p450-aktivität
WO2004059294A2 (en) * 2002-12-23 2004-07-15 Promega Corporation Improved luciferase-based assays
JP2006517413A (ja) * 2003-02-12 2006-07-27 プロメガ　コーポレイション 光を発光反応から消失させる二重酵素アッセイの方法及びキット
EP2778234B1 (de) 2005-05-31 2017-09-27 Promega Corporation Luminogene und fluorogene verbindungen und verfahren für den nachweis von molekülen oder leiden
WO2008045121A1 (en) * 2006-10-13 2008-04-17 Charm Sciences, Inc. Method and apparatus for reducing luminescent test result interferences
US20080248511A1 (en) * 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
WO2009032218A1 (en) * 2007-08-29 2009-03-12 Biolume Inc. Bioluminescent endoscopy methods and compounds
AU2008319571B2 (en) * 2007-10-29 2013-11-28 Revvity Health Sciences B.V. Methods, reagents and kits for luciferase assay
WO2010021686A1 (en) * 2008-08-18 2010-02-25 Promega Corporation Luminogenic compounds and methods to detect cytochrome p450 3a enzymes
ES2795287T3 (es) 2009-05-01 2020-11-23 Promega Corp Luciferasas de Oplophorus sintéticas con mayor emisión de luz
CN103443121B (zh) 2010-11-02 2016-04-20 普罗梅加公司 新型腔肠素底物及其使用方法
CA2815381C (en) 2010-11-02 2019-03-19 Promega Corporation Coelenterazine derivatives and methods of using same
EP3228714B1 (de) * 2010-12-14 2019-03-27 Stichting Katholieke Universiteit Chemilumineszenzbasierter hämostasetest
US20120204906A1 (en) * 2011-02-15 2012-08-16 3M Innovative Properties Company Method of controlling a decontamination process
CN104755931B (zh) 2012-05-02 2016-08-31 查尔斯河实验室公司 用于检测细胞样品中的活细胞的方法
US10324036B2 (en) 2012-05-02 2019-06-18 Charles River Laboratories, Inc. Porous planar cell capture system
JP6285915B2 (ja) 2012-05-02 2018-02-28 チャールズ リバー ラボラトリーズ， インコーポレイテッド 生死判別染色法
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
EP3099691B1 (de) 2014-01-29 2019-11-20 Promega Corporation Pro-substrate für lebendzellanwendungen
CN108779452B (zh) 2016-03-11 2022-05-03 国立研究开发法人产业技术综合研究所 发光酶蛋白
EP3661932B1 (de) 2017-08-04 2021-10-06 Promega Corporation Zusammensetzungen und verfahren zur stabilisierung von benzothiazol-luciferin-analoga
AU2020272037B2 (en) * 2019-04-10 2025-11-27 Promega Corporation Compositions and methods for analyte detection using bioluminescence
WO2024262411A1 (ja) * 2023-06-22 2024-12-26 国立研究開発法人産業技術総合研究所 血清アルブミンの検出剤
CN119551880B (zh) * 2025-02-06 2025-04-08 山东黄金矿业科技有限公司选冶实验室分公司 一种含氰废水中硫氰根的脱除方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE428379B (sv) 1978-05-31 1983-06-27 Lkb Produkter Ab Bioluminiscens bestemning av atp och reagens herfor
DE2828658C3 (de) 1978-06-29 1981-10-22 Lkb-Produkter Ab, Stockholm Verfahren zur photometrischen Bestimmung der Untereinheit B der Creatinkinase und Reagens hierfür
DE2901919C2 (de) 1979-01-18 1987-04-02 Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad Verfahren zur photometrischen Reihenmessung an Reaktionsvorgängen in flüssigen Proben und Vorrichtung zur Durchführung des Verfahrens
SE432112B (sv) * 1979-07-12 1984-03-19 Lkb Produkter Ab Forfarande for bestemning av kreatinkinas i prover innehallande atp
DE3048884A1 (de) 1980-12-23 1982-07-15 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur enzymimmunobestimmung in heterogener phase
US4650770A (en) * 1981-04-27 1987-03-17 Syntex (U.S.A.) Inc. Energy absorbing particle quenching in light emitting competitive protein binding assays
WO1987007386A1 (en) 1986-05-22 1987-12-03 Unilever Plc Solid phase immunoassay method
GB8811294D0 (en) 1988-05-12 1988-06-15 Kabivitrum Peptide Hormones Ab Luminescent/luminometric assays
ATE137805T1 (de) * 1989-02-14 1996-05-15 Wako Pure Chem Ind Ltd Verfahren zur erhöhung von chemilumineszenz
FI901681A7 (fi) * 1989-04-10 1990-10-11 Ela Tech Inc Menetelmä luminesenssianalyysien herkkyyden lisäämiseksi
JPH03167288A (ja) 1989-11-27 1991-07-19 Chisso Corp 界面活性剤によるエクオリンの増感発光法
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
US5629168A (en) * 1992-02-10 1997-05-13 British Technology Group Limited Chemiluminescent enhancers
AT401526B (de) 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
CA2104815A1 (en) * 1993-02-26 1994-08-27 Naotaka Kuroda Method for measuring adenyl group-containing substances
JPH0767696A (ja) 1993-09-06 1995-03-14 Tosoh Corp バックグランド発光の低減法
US5744320A (en) * 1995-06-07 1998-04-28 Promega Corporation Quenching reagents and assays for enzyme-mediated luminescence
US6602657B1 (en) 1995-12-28 2003-08-05 Tropix, Inc. Multiple reporter gene assay
US5795729A (en) * 1996-02-05 1998-08-18 Biometric Imaging, Inc. Reductive, energy-transfer fluorogenic probes
US6416960B1 (en) * 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
US5814504A (en) * 1996-08-22 1998-09-29 Kikkoman Corporation Protein involved in regenerating firefly luciferin
JPH1087621A (ja) 1996-09-13 1998-04-07 Sankyo Co Ltd ルシゲニン化学発光の増強剤
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
DK1081495T3 (da) 1999-09-01 2004-06-14 Invitrogen Corp Fotondæmpende midler til fluorescensassays
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
JP2006517413A (ja) 2003-02-12 2006-07-27 プロメガ　コーポレイション 光を発光反応から消失させる二重酵素アッセイの方法及びキット

Also Published As

Publication number Publication date
WO2001096862A2 (en) 2001-12-20
CA2411179A1 (en) 2001-12-20
DE60143888D1 (de) 2011-03-03
US20040096924A1 (en) 2004-05-20
EP1297337B1 (de) 2011-01-19
AU2001275325B2 (en) 2006-10-05
JP4685325B2 (ja) 2011-05-18
WO2001096862A3 (en) 2002-07-18
US20060051827A1 (en) 2006-03-09
AU7532501A (en) 2001-12-24
JP2004503777A (ja) 2004-02-05
US7118878B1 (en) 2006-10-10
US7078181B2 (en) 2006-07-18
EP1297337A2 (de) 2003-04-02
US7108996B2 (en) 2006-09-19
JP2011064690A (ja) 2011-03-31

Similar Documents

Publication Publication Date Title
DE60143888D1 (de) Methode um die sensitivität einer luminesenz assay zu erhöhen
WO2004059294A3 (en) Improved luciferase-based assays
ATE239912T1 (de) Verfahren und vorrichtung zur referenzierung von fluoreszenzintensitätssignalen
DK0944731T3 (da) Enzymsensor
DK0539477T3 (da) Fremgangsmåde til forstærkning af emissionssignalet af en luminescerende forbindelse
DK0716740T3 (da) Fremgangsmåde til aktivering af polyanioniske, fluorescerende farvestoffer i medier med lav dielektricitet ved hjælp af kvaternære oniumforbindelser
PT1040353E (pt) Metodo de despistagem de um analito e estojo
ATE545460T1 (de) Proteomanalyseverfahren für phosphoryliertes protein
AU3221101A (en) Detection of binding of charged species using pH- or potential-sensitive probes
CY1105025T1 (el) Αναλυση λεπτινης βασιζομενη στην ανιχνευση εκφρασης ang-2
GB0126319D0 (en) Microfluidic serrs detection
ATE377094T1 (de) Detektion von wechselwirkungen auf sonden-arrays
DE60308454D1 (de) Fluoreszenzmarkierten Hybridisierungssonden mit reduzierter Hintergrundfluoreszenz
SE0004334D0 (sv) Electron spectroscopy
ATE368222T1 (de) Entglykosilierte enzymen für konjugaten
WO2004047614A3 (en) Methods, biosensors and kits for detecting and identifying fungi
DE60332479D1 (de) Holo-transcobalamin bindungspartner und ihre verwendung in cobalamintest
WO2003016903A8 (de) Vorrichtung und verfahren zum nachweis eines analyten
EA200400024A1 (ru) Новый сцинтилляционный анализ близкого расстояния для изучения связывающих аминогликозид молекул (abm)
EP1575997A4 (de) Phosphospezifische pak-antikörper unddiagnostische kits
ES2192618T3 (es) Metodo de seleccion.
WO2005001124A3 (en) Methods of nucleic acid analysis
DE59813125D1 (de) Srcr domäne-enthaltendes protein
DE59910326D1 (de) Verwendung derivatisierter Reagenzien in Chemilumineszenz- und Elektrochemilumineszenz-Nachweisverfahren
DK1297342T3 (da) Metode til diagnostisk bestemmelse af Alzheimer's sygdom på basis af holo-transcobalamin II

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties