ATE500323T1 - Subtilisin-variante - Google Patents

Subtilisin-variante

Info

Publication number
ATE500323T1
ATE500323T1 AT01923084T AT01923084T ATE500323T1 AT E500323 T1 ATE500323 T1 AT E500323T1 AT 01923084 T AT01923084 T AT 01923084T AT 01923084 T AT01923084 T AT 01923084T AT E500323 T1 ATE500323 T1 AT E500323T1
Authority
AT
Austria
Prior art keywords
homologues
new
subtilisin variant
proteins
subtilisin
Prior art date
Application number
AT01923084T
Other languages
English (en)
Inventor
Jon Ness
Mark Welch
Lorraine Giver
Joel Cherry
Torben Borchert
Willem Stemmer
Jeremy Minshull
Original Assignee
Maxygen Inc
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Novozymes As filed Critical Maxygen Inc
Application granted granted Critical
Publication of ATE500323T1 publication Critical patent/ATE500323T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT01923084T 2000-04-03 2001-04-02 Subtilisin-variante ATE500323T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19414300P 2000-04-03 2000-04-03
PCT/US2001/010781 WO2001075087A2 (en) 2000-04-03 2001-04-02 Subtilisin variants

Publications (1)

Publication Number Publication Date
ATE500323T1 true ATE500323T1 (de) 2011-03-15

Family

ID=22716459

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01923084T ATE500323T1 (de) 2000-04-03 2001-04-02 Subtilisin-variante

Country Status (8)

Country Link
US (4) US6902922B2 (de)
EP (1) EP1287123B1 (de)
JP (1) JP2004508011A (de)
AT (1) ATE500323T1 (de)
AU (4) AU2001249811B2 (de)
CA (1) CA2405063C (de)
DE (1) DE60144145D1 (de)
WO (1) WO2001075087A2 (de)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
AU2001249811B2 (en) * 2000-04-03 2006-06-08 Maxygen, Inc. Subtilisin variants
US7226770B2 (en) * 2000-04-28 2007-06-05 Novozymes A/S Lipolytic enzyme variant
US7109016B2 (en) * 2000-08-21 2006-09-19 Novozymes A/S Subtilase enzymes
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP5328002B2 (ja) * 2002-01-16 2013-10-30 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
ATE450606T1 (de) * 2002-01-16 2009-12-15 Genencor Int Mehrfachsubstituierte proteasevarianten
ATE356871T1 (de) 2002-05-17 2007-04-15 Alligator Bioscience Ab Eine methode zur in vitro molekularen evolution der proteinfunktion
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
JP4819670B2 (ja) * 2003-05-07 2011-11-24 ノボザイムス アクティーゼルスカブ 変異体スブチリシン酵素（サブチラーゼ）
US9695251B2 (en) 2003-10-31 2017-07-04 The Regents Of The University Of California Activatable cell penetrating peptides with quenched fluorophores
US7824896B2 (en) * 2004-04-02 2010-11-02 Novozymes A/S Subtilase variants having altered lmmunogenicity
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
EP2013339B1 (de) 2006-04-20 2013-09-11 Novozymes A/S Savinase-varianten mit verbesserter waschleistung gegen eiflecken
US7802883B2 (en) * 2007-12-20 2010-09-28 Johnson & Johnson Vision Care, Inc. Cosmetic contact lenses having a sparkle effect
BRPI0915681A2 (pt) 2008-06-06 2015-08-04 Danisco Us Inc Composições e métodos compreendendo proteases microbianas variantes
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
ES2705694T3 (es) 2008-06-13 2019-03-26 Codexis Inc Método de síntesis de variantes de polinucleótidos
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
DK3301208T3 (da) 2009-09-18 2020-12-14 Codexis Inc Reduceret codon-mutagenese
WO2015004241A2 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
KR102382489B1 (ko) 2016-05-05 2022-04-01 코덱시스, 인코포레이티드 페니실린 ｇ 아실라제
KR20260006703A (ko) 2017-02-03 2026-01-13 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
AU2018217495B2 (en) 2017-02-13 2021-03-04 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
JP7045725B2 (ja) 2017-04-27 2022-04-01 コデクシス， インコーポレイテッド ケトレダクターゼポリペプチドおよびポリヌクレオチド
CA3062550A1 (en) 2017-05-08 2018-11-15 Codexis, Inc. Engineered ligase variants
WO2018231462A1 (en) 2017-06-14 2018-12-20 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
EP3645713A4 (de) 2017-06-27 2021-06-30 Codexis, Inc. Penicillin-g-acylasen
SG11202003094PA (en) 2017-11-07 2020-05-28 Codexis Inc Transglutaminase variants
AU2018383752B2 (en) 2017-12-13 2025-01-30 Codexis, Inc. Carboxyesterase polypeptides for amide coupling
EP3807409A4 (de) 2018-06-12 2022-08-03 Codexis, Inc. Manipulierte tyrosin-ammoniak-lyase
EP3821000A4 (de) 2018-07-09 2022-07-20 Codexis, Inc. Manipulierte galaktose-oxidase-enzymvarianten
CN112673105A (zh) 2018-07-09 2021-04-16 科德克希思公司 工程化脱氧核糖磷酸醛缩酶
JP7461658B2 (ja) 2018-07-09 2024-04-04 コデクシス， インコーポレイテッド 操作されたプリンヌクレオシドホスホリラーゼバリアント酵素
JP7491588B2 (ja) 2018-07-09 2024-05-28 コデクシス， インコーポレイテッド 操作されたパントテン酸キナーゼ改変体酵素
AU2019302422B2 (en) 2018-07-09 2022-08-04 Codexis, Inc. Engineered phosphopentomutase variant enzymes
EP3820833A4 (de) 2018-07-12 2022-08-03 Codexis, Inc. Manipulierte phenylalanin-ammoniak-lyase-polypeptide
IL319918A (en) 2018-07-30 2025-05-01 Tate & Lyle Solutions Usa Llc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
JP7780186B2 (ja) 2018-10-29 2025-12-04 コデクシス， インコーポレイテッド 操作されたｄｎａポリメラーゼバリアント
AU2019397401A1 (en) 2018-12-14 2021-06-17 Codexis, Inc. Engineered tyrosine ammonia lyase
CN113396218B (zh) 2019-01-15 2025-02-18 科德克希思公司 工程化转氨酶多肽
JP7245676B2 (ja) * 2019-03-11 2023-03-24 花王株式会社 変異プロテアーゼ
US12215359B2 (en) 2019-05-01 2025-02-04 Codexis, Inc. Engineered glucose dehydrogenases and methods for the reductive amination of ketone and amine compounds
EP3963057A4 (de) 2019-05-01 2023-06-14 Codexis, Inc. Manipulierte iminreduktasen und verfahren zur reduktiven aminierung von keton- und aminverbindungen
KR20220025884A (ko) 2019-07-02 2022-03-03 코덱시스, 인코포레이티드 조작된 아세테이트 키나제 변이체 효소
AU2020300454A1 (en) 2019-07-02 2021-12-23 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
BR112022003705A2 (pt) 2019-08-30 2022-05-24 Codexis Inc Lipase recombinante, composição, sequência de polinucleotídeo recombinante, vetor de expressão, composição farmacêutica para o tratamento de insuficiência pancreática, célula hospedeira, métodos para produzir uma lipase recombinante e para tratar e/ou prevenir os sintomas de insuficiência pancreática, e, uso
CN114760980B (zh) 2019-09-12 2024-10-18 科德克希思公司 针对10-乙酰基-3,7-二羟基吩噁嗪的过氧化物酶活性
IL293038A (en) 2019-11-26 2022-07-01 Codexis Inc Biocatalysts and methods for hydroxylation of chemical compounds
MX2022007399A (es) 2019-12-20 2022-09-19 Codexis Inc Variantes de alfa-glucosidasa ácida diseñadas.
EP4103711A4 (de) 2020-02-04 2024-02-28 Codexis, Inc. Gentechnisch veränderte leucindecarboxylasen
US11788071B2 (en) 2020-04-10 2023-10-17 Codexis, Inc. Engineered transaminase polypeptides
CA3179679A1 (en) 2020-04-10 2021-10-14 Codexis, Inc. Carboxyesterase polypeptides for kinetic resolution
AU2021334371A1 (en) 2020-08-28 2023-03-09 Codexis, Inc. Engineered protease variants
JP2023539634A (ja) 2020-08-28 2023-09-15 コデクシス， インコーポレイテッド 操作されたアミラーゼバリアント
US20240240169A1 (en) * 2020-09-21 2024-07-18 Bayer Cropscience Lp Novel serine proteases
CN116209754A (zh) 2020-09-28 2023-06-02 科德克希思公司 用于合成手性胺的工程化生物催化剂及方法
CN116710558A (zh) 2020-10-07 2023-09-05 科德克希思公司 工程化磷酸戊糖变位酶变体酶
WO2022133289A2 (en) 2020-12-18 2022-06-23 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2022212832A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
US11913037B2 (en) 2021-04-02 2024-02-27 Codexis, Inc. Engineered adenylate kinase variant enzymes
IL305922A (en) 2021-04-02 2023-11-01 Codexis Inc Transgenic enzymes of variant guanylate kinase
EP4314261A1 (de) 2021-04-02 2024-02-07 Codexis, Inc. Manipulierte enzyme mit acetatkinasevarianten
AU2022279241A1 (en) 2021-05-21 2023-11-30 Syntis Bio, Inc. Engineered methionine gamma lyase variants
WO2023069865A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
WO2023064938A1 (en) 2021-10-15 2023-04-20 Codexis, Inc. Engineered dna polymerase variants
JP2024540358A (ja) 2021-11-01 2024-10-31 シンティス バイオ，インコーポレイティド 工学操作ロイシン脱炭酸酵素
WO2025235589A1 (en) 2024-05-08 2025-11-13 Codexis, Inc. Dna polymerase variants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US49827A (en) * 1865-09-05 Improvement in machines for making cement pipe
PT86864B (pt) * 1987-02-27 1992-05-29 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
KR100258460B1 (ko) * 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
KR950702633A (ko) * 1992-07-17 1995-07-29 한스 발터 라벤 고알칼리성 세린 프로테아제(high alkaline serine proteases)
DE69331907T2 (de) * 1992-09-24 2002-09-05 Genencor International, Inc. Neue alkalische proteasen enthaltende reinigungsmittelzusammensetzungen
ATE245185T1 (de) * 1993-10-14 2003-08-15 Procter & Gamble Proteasen enthaltende bleichmittelzusammensetzungen
HU219851B (hu) 1993-10-14 2001-08-28 The Procter And Gamble Company Proteáz tartalmú tisztítókészítmények
US5972042A (en) 1995-12-22 1999-10-26 Novo Nordisk A/S Method for dyeing a material with a dyeing system which contains an enzymatic oxidizing agent
KR100591553B1 (ko) * 1996-11-04 2006-06-19 노보자임스 에이/에스 섭틸라제 변종과 조성물
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
AU2001249811B2 (en) * 2000-04-03 2006-06-08 Maxygen, Inc. Subtilisin variants
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162728A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Also Published As

Publication number Publication date
US6902922B2 (en) 2005-06-07
US20030049827A1 (en) 2003-03-13
US7605115B2 (en) 2009-10-20
WO2001075087A2 (en) 2001-10-11
US20070270574A1 (en) 2007-11-22
CA2405063C (en) 2013-06-04
AU2006208427B2 (en) 2009-10-01
CA2405063A1 (en) 2001-10-11
US7220566B2 (en) 2007-05-22
AU2009251169A1 (en) 2010-01-21
DE60144145D1 (de) 2011-04-14
AU2001249811B2 (en) 2006-06-08
AU4981101A (en) 2001-10-15
US20040203130A1 (en) 2004-10-14
JP2004508011A (ja) 2004-03-18
EP1287123B1 (de) 2011-03-02
US20100105599A1 (en) 2010-04-29
WO2001075087A8 (en) 2001-12-27
WO2001075087A3 (en) 2002-12-19
EP1287123A2 (de) 2003-03-05
AU2006208427A1 (en) 2006-09-28

Similar Documents

Publication Publication Date Title
DE60144145D1 (de) Subtilisin-variante
WO2002006457A3 (en) Novel lipase genes
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
DE60127441D1 (de) Dipeptidylpeptidasen
DE69942459D1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
MXPA03002218A (es) Peptidos antimicrobianos y metodos de uso.
AR006188A1 (es) "composicion farmaceutica para inhibir la actividad de serinproteasa, metodo in vitro para inhibir la actividad de serinproteasa y metodo para preparar dicha composicion".
ATE380822T1 (de) Funf-helix protein
WO2002031134A3 (en) Novel serine protease genes related to dppiv
DE60134786D1 (de) Mini-dystrophin nukleinsäure- und peptidsequenzen
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
DK0981630T3 (da) Polypeptider med prolyldipeptidylaminopeptidaseaktivitet og nukleinsyrer, der koder for samme
ATE474926T1 (de) Verwendung von clya hemolysin fur ausscheidung von fusionprotein
DE69535696D1 (de) Tripeptidyl-aminopeptidase
WO2001025438A3 (en) Ifn-alpha homologues
DE60139544D1 (de) Veränderte, fluoreszierende proteine
DK1082423T3 (da) Cyclin E2-gener og -proteiner
WO2001064912A3 (en) Triazine degrading enzymes
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
DE69734586D1 (de) Retinoid-metabolisierendes protein
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
ATE341339T1 (de) Zusammensetzungen von rekombinanten papillomavirus vakzinen
SE9602822D0 (sv) New receptor
WO2002062964A3 (en) Ap1 amine oxidase variants
WO2001040257A3 (en) Modified il-12 subunits and nucleic acids encoding them

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties