ATE500323T1 - Subtilisin-variante - Google Patents

Subtilisin-variante

Info

Publication number
ATE500323T1
ATE500323T1 AT01923084T AT01923084T ATE500323T1 AT E500323 T1 ATE500323 T1 AT E500323T1 AT 01923084 T AT01923084 T AT 01923084T AT 01923084 T AT01923084 T AT 01923084T AT E500323 T1 ATE500323 T1 AT E500323T1
Authority
AT
Austria
Prior art keywords
homologues
new
subtilisin variant
proteins
subtilisin
Prior art date
Application number
AT01923084T
Other languages
English (en)
Inventor
Jon Ness
Mark Welch
Lorraine Giver
Joel Cherry
Torben Borchert
Willem Stemmer
Jeremy Minshull
Original Assignee
Maxygen Inc
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Novozymes As filed Critical Maxygen Inc
Application granted granted Critical
Publication of ATE500323T1 publication Critical patent/ATE500323T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT01923084T 2000-04-03 2001-04-02 Subtilisin-variante ATE500323T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19414300P 2000-04-03 2000-04-03
PCT/US2001/010781 WO2001075087A2 (en) 2000-04-03 2001-04-02 Subtilisin variants

Publications (1)

Publication Number Publication Date
ATE500323T1 true ATE500323T1 (de) 2011-03-15

Family

ID=22716459

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01923084T ATE500323T1 (de) 2000-04-03 2001-04-02 Subtilisin-variante

Country Status (8)

Country Link
US (4) US6902922B2 (de)
EP (1) EP1287123B1 (de)
JP (1) JP2004508011A (de)
AT (1) ATE500323T1 (de)
AU (4) AU4981101A (de)
CA (1) CA2405063C (de)
DE (1) DE60144145D1 (de)
WO (1) WO2001075087A2 (de)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
JP2004508011A (ja) * 2000-04-03 2004-03-18 マキシジェン，　インコーポレイテッド スブチリシン変異体
EP1280817A2 (de) * 2000-04-28 2003-02-05 Novozymes A/S Identifizierung, produktion und verwendung von proteinvarianten mit veränderten immunogenzität
US7109016B2 (en) * 2000-08-21 2006-09-19 Novozymes A/S Subtilase enzymes
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
EP2287320B1 (de) * 2002-01-16 2014-10-01 Danisco US Inc. Mehrfach substituierte Proteasevarianten
ES2336092T3 (es) * 2002-01-16 2010-04-08 Genencor International, Inc. Variantes de proteasas con multiples sustituciones.
DK1504098T4 (da) 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
JP4819670B2 (ja) * 2003-05-07 2011-11-24 ノボザイムス アクティーゼルスカブ 変異体スブチリシン酵素（サブチラーゼ）
US9695251B2 (en) 2003-10-31 2017-07-04 The Regents Of The University Of California Activatable cell penetrating peptides with quenched fluorophores
US7824896B2 (en) * 2004-04-02 2010-11-02 Novozymes A/S Subtilase variants having altered lmmunogenicity
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
US20090215663A1 (en) 2006-04-20 2009-08-27 Novozymes A/S Savinase variants having an improved wash performance on egg stains
US7802883B2 (en) 2007-12-20 2010-09-28 Johnson & Johnson Vision Care, Inc. Cosmetic contact lenses having a sparkle effect
CN102046783A (zh) * 2008-06-06 2011-05-04 丹尼斯科美国公司 包含变体微生物蛋白酶的组合物和方法
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
DK3023494T3 (en) 2008-06-13 2019-02-25 Codexis Inc PROCEDURE FOR SYNTHESIS OF POLYNUCLEOTIDE VARIETIES
DK3301208T3 (da) 2009-09-18 2020-12-14 Codexis Inc Reduceret codon-mutagenese
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
SG11201600115SA (en) 2013-07-10 2016-02-26 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
US10724025B2 (en) 2016-05-05 2020-07-28 Codexis, Inc. Penicillin-G acylases
CA3175070A1 (en) 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
MX2019009615A (es) 2017-02-13 2019-10-14 Codexis Inc Polipeptidos dise?ados de fenilalanina amonio liasa.
CN110831618B (zh) 2017-04-27 2023-08-25 科德克希思公司 酮还原酶多肽及多核苷酸
ES2887078T3 (es) 2017-05-08 2021-12-21 Codexis Inc Variantes de ligasa diseñadas
EP3638688A4 (de) 2017-06-14 2021-07-07 Codexis, Inc. Manipulierte transaminase-polypeptide für industrielle biokatalyse
CN117737045A (zh) 2017-06-27 2024-03-22 科德克希思公司 青霉素g酰化酶
EP3706780A4 (de) 2017-11-07 2021-12-22 Codexis, Inc. Transglutaminasevarianten
JP2021506252A (ja) 2017-12-13 2021-02-22 コデクシス， インコーポレイテッド アミドカップリングのためのカルボキシエステラーゼポリペプチド
US10900055B2 (en) 2018-06-12 2021-01-26 Codexis, Inc. Engineered tyrosine ammonia lyase
EP3821015A4 (de) 2018-07-09 2022-07-13 Codexis, Inc. Gentechnisch hergestellte desoxyribose-phosphat-aldolasen
IL279884B2 (en) 2018-07-09 2026-04-01 Codexis Inc Engineered pantothenate kinase variant enzymes
SG11202012138XA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered galactose oxidase variant enzymes
AU2019302423B2 (en) 2018-07-09 2024-11-21 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
EP3821026A4 (de) 2018-07-09 2022-06-01 Codexis, Inc. Manipulierte enzymvarianten der phosphopentomutase
JP2021531749A (ja) 2018-07-12 2021-11-25 コデクシス， インコーポレイテッド 操作されたフェニルアラニンアンモニアリアーゼポリペプチド
WO2020028039A1 (en) 2018-07-30 2020-02-06 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
AU2019373208A1 (en) 2018-10-29 2021-05-13 Codexis, Inc. Engineered DNA polymerase variants
CA3122524A1 (en) 2018-12-14 2020-06-18 Codexis, Inc. Engineered tyrosine ammonia lyase
CA3126671A1 (en) 2019-01-15 2020-07-23 Codexis, Inc. Engineered transaminase polypeptides
JP7245676B2 (ja) 2019-03-11 2023-03-24 花王株式会社 変異プロテアーゼ
EP3963057A4 (de) 2019-05-01 2023-06-14 Codexis, Inc. Manipulierte iminreduktasen und verfahren zur reduktiven aminierung von keton- und aminverbindungen
CN114096662B (zh) 2019-05-01 2025-04-15 科德克希思公司 工程化葡萄糖脱氢酶和用于酮和胺化合物的还原胺化的方法
WO2021003132A1 (en) 2019-07-02 2021-01-07 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
AU2020299358A1 (en) 2019-07-02 2021-12-23 Codexis, Inc. Engineered acetate kinase variant enzymes
EP4501403A3 (de) 2019-08-30 2025-05-14 Société des Produits Nestlé S.A. Manipulierte lipasevarianten
US12012620B2 (en) 2019-09-12 2024-06-18 Codexis, Inc. Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine
CN121780457A (zh) 2019-11-26 2026-04-03 科德克希思公司 用于化学化合物的羟基化的生物催化剂和方法
JP2023507575A (ja) 2019-12-20 2023-02-24 コデクシス， インコーポレイテッド 操作された酸性アルファ－グルコシダーゼバリアント
JP2023512683A (ja) 2020-02-04 2023-03-28 コデクシス， インコーポレイテッド 操作されたロイシンデカルボキシラーゼ
EP4133064A4 (de) 2020-04-10 2024-07-24 Codexis, Inc. Gentechnisch hergestellte transaminasepolypeptide
JP7747337B2 (ja) 2020-04-10 2025-10-01 コデクシス， インコーポレイテッド 速度論的光学分割のためのカルボキシエステラーゼポリペプチド
CN116897205A (zh) 2020-08-28 2023-10-17 科德克希思公司 工程化淀粉酶变体
AU2021334371A1 (en) 2020-08-28 2023-03-09 Codexis, Inc. Engineered protease variants
BR112023004843A2 (pt) * 2020-09-21 2023-04-18 Bayer Cropscience Lp Serina proteases
WO2022133289A2 (en) 2020-12-18 2022-06-23 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
CA3215105A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered acetate kinase variant enzymes
US12180483B2 (en) 2021-04-02 2024-12-31 Codexis, Inc. Engineered cyclic GMP-AMP synthase (cGAS) variant enzymes
IL305919A (en) 2021-04-02 2023-11-01 Codexis Inc Engineered adenylate kinase variant enzymes
EP4314262A1 (de) 2021-04-02 2024-02-07 Codexis, Inc. Manipulierte guanylatkinase-variantenenzyme
CA3220735A1 (en) 2021-05-21 2022-11-24 Codexis, Inc. Engineered methionine gamma lyase variants
CA3234383A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
US12129495B2 (en) 2021-10-15 2024-10-29 Codexis, Inc. Engineered DNA polymerase variants
JP2024540358A (ja) 2021-11-01 2024-10-31 シンティス バイオ，インコーポレイティド 工学操作ロイシン脱炭酸酵素
EP4525615A2 (de) 2022-05-14 2025-03-26 Novozymes A/S Zusammensetzungen und verfahren zur prävention, behandlung, unterdrückung und/oder beseitigung von phytopathogenen befällen und infektionen
WO2025235589A1 (en) 2024-05-08 2025-11-13 Codexis, Inc. Dna polymerase variants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US49827A (en) * 1865-09-05 Improvement in machines for making cement pipe
WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
ES2121014T3 (es) * 1991-05-01 1998-11-16 Novo Nordisk As Enzimas estabilizadas y composiciones detergentes.
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5316935A (en) 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
KR950702633A (ko) 1992-07-17 1995-07-29 한스 발터 라벤 고알칼리성 세린 프로테아제(high alkaline serine proteases)
DE69331907T2 (de) * 1992-09-24 2002-09-05 Genencor International, Inc. Neue alkalische proteasen enthaltende reinigungsmittelzusammensetzungen
EP0723580B1 (de) * 1993-10-14 2003-07-16 The Procter & Gamble Company Proteasen enthaltende bleichmittelzusammensetzungen
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
US5972042A (en) * 1995-12-22 1999-10-26 Novo Nordisk A/S Method for dyeing a material with a dyeing system which contains an enzymatic oxidizing agent
EP0932667B1 (de) * 1996-11-04 2008-10-01 Novozymes A/S Subtilase varianten und verbindungen
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
JP2004508011A (ja) * 2000-04-03 2004-03-18 マキシジェン，　インコーポレイテッド スブチリシン変異体
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162728A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Also Published As

Publication number Publication date
US7220566B2 (en) 2007-05-22
EP1287123A2 (de) 2003-03-05
US20030049827A1 (en) 2003-03-13
US20100105599A1 (en) 2010-04-29
AU2006208427A1 (en) 2006-09-28
US7605115B2 (en) 2009-10-20
WO2001075087A2 (en) 2001-10-11
AU4981101A (en) 2001-10-15
US20070270574A1 (en) 2007-11-22
AU2006208427B2 (en) 2009-10-01
CA2405063A1 (en) 2001-10-11
CA2405063C (en) 2013-06-04
JP2004508011A (ja) 2004-03-18
WO2001075087A8 (en) 2001-12-27
AU2009251169A1 (en) 2010-01-21
WO2001075087A3 (en) 2002-12-19
DE60144145D1 (de) 2011-04-14
AU2001249811B2 (en) 2006-06-08
EP1287123B1 (de) 2011-03-02
US20040203130A1 (en) 2004-10-14
US6902922B2 (en) 2005-06-07

Similar Documents

Publication Publication Date Title
DE60144145D1 (de) Subtilisin-variante
WO2002006457A3 (en) Novel lipase genes
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
DE60127441D1 (de) Dipeptidylpeptidasen
DE69942459D1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
AR006188A1 (es) "composicion farmaceutica para inhibir la actividad de serinproteasa, metodo in vitro para inhibir la actividad de serinproteasa y metodo para preparar dicha composicion".
DE60037450D1 (de) Funf-helix protein
WO2002031134A3 (en) Novel serine protease genes related to dppiv
DE60134786D1 (de) Mini-dystrophin nukleinsäure- und peptidsequenzen
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
DK0981630T3 (da) Polypeptider med prolyldipeptidylaminopeptidaseaktivitet og nukleinsyrer, der koder for samme
ATE474926T1 (de) Verwendung von clya hemolysin fur ausscheidung von fusionprotein
ATE384787T1 (de) Tripeptidyl-aminopeptidase
ATE439441T1 (de) Veränderte, fluoreszierende proteine
DK1082423T3 (da) Cyclin E2-gener og -proteiner
WO2001064912A3 (en) Triazine degrading enzymes
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
ATE309370T1 (de) Retinoid-metabolisierendes protein
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
ATE341339T1 (de) Zusammensetzungen von rekombinanten papillomavirus vakzinen
SE9602822D0 (sv) New receptor
WO2002062964A3 (en) Ap1 amine oxidase variants
WO2001040257A3 (en) Modified il-12 subunits and nucleic acids encoding them
WO2001096593A3 (en) Novel motor proteins and methods for their use
WO2002012541A3 (en) Novel motor proteins and methods for their use

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties