ATE500323T1 - Subtilisin-variante - Google Patents

Subtilisin-variante

Info

Publication number
ATE500323T1
ATE500323T1 AT01923084T AT01923084T ATE500323T1 AT E500323 T1 ATE500323 T1 AT E500323T1 AT 01923084 T AT01923084 T AT 01923084T AT 01923084 T AT01923084 T AT 01923084T AT E500323 T1 ATE500323 T1 AT E500323T1
Authority
AT
Austria
Prior art keywords
homologues
new
subtilisin variant
proteins
subtilisin
Prior art date
Application number
AT01923084T
Other languages
English (en)
Inventor
Jon Ness
Mark Welch
Lorraine Giver
Joel Cherry
Torben Borchert
Willem Stemmer
Jeremy Minshull
Original Assignee
Maxygen Inc
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Novozymes As filed Critical Maxygen Inc
Application granted granted Critical
Publication of ATE500323T1 publication Critical patent/ATE500323T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT01923084T 2000-04-03 2001-04-02 Subtilisin-variante ATE500323T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19414300P 2000-04-03 2000-04-03
PCT/US2001/010781 WO2001075087A2 (en) 2000-04-03 2001-04-02 Subtilisin variants

Publications (1)

Publication Number Publication Date
ATE500323T1 true ATE500323T1 (de) 2011-03-15

Family

ID=22716459

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01923084T ATE500323T1 (de) 2000-04-03 2001-04-02 Subtilisin-variante

Country Status (8)

Country Link
US (4) US6902922B2 (de)
EP (1) EP1287123B1 (de)
JP (1) JP2004508011A (de)
AT (1) ATE500323T1 (de)
AU (4) AU2001249811B2 (de)
CA (1) CA2405063C (de)
DE (1) DE60144145D1 (de)
WO (1) WO2001075087A2 (de)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
WO2001083559A2 (en) * 2000-04-28 2001-11-08 Novozymes A/S Production and use of protein variants having modified immunogenecity
US7109016B2 (en) * 2000-08-21 2006-09-19 Novozymes A/S Subtilase enzymes
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162727A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
CA2472725C (en) * 2002-01-16 2015-08-11 Genencor International, Inc. Multiply-substituted protease variants
EP1530631B1 (de) * 2002-01-16 2013-08-07 Genencor International, Inc. Mehrfachsubstituierte proteasevarianten
EP1504098B2 (de) 2002-05-17 2011-02-09 Alligator Bioscience AB Eine methode zur in vitro molekularen evolution der proteinfunktion
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
BRPI0409992A (pt) * 2003-05-07 2006-05-09 Novozymes As enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar
US9695251B2 (en) 2003-10-31 2017-07-04 The Regents Of The University Of California Activatable cell penetrating peptides with quenched fluorophores
ATE491025T1 (de) * 2004-04-02 2010-12-15 Novozymes As Subtilasevarianten mit veränderter immunogenität
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
JP2009534022A (ja) 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ 卵のシミに対する洗浄能力が向上したサビナーゼ変異体
US7802883B2 (en) 2007-12-20 2010-09-28 Johnson & Johnson Vision Care, Inc. Cosmetic contact lenses having a sparkle effect
CN104694519B (zh) * 2008-06-06 2023-10-27 丹尼斯科美国公司 包含微生物蛋白酶变体的组合物和方法
EP2285958B1 (de) 2008-06-13 2016-03-09 Codexis, Inc. Verfahren zur synthese von polynukleotidvarianten
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
HUE037123T2 (hu) 2009-09-18 2018-08-28 Codexis Inc Csökkentett kodon mutagenezis
KR20160035587A (ko) 2013-07-10 2016-03-31 노파르티스 아게 복수개의 프로테아제 결핍 사상형 진균 세포들 및 그의 이용방법
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
WO2017193022A1 (en) 2016-05-05 2017-11-09 Codexis, Inc. Penicillin-g acylases
WO2018144679A2 (en) 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
CA3051262A1 (en) 2017-02-13 2018-08-16 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
CN110831618B (zh) 2017-04-27 2023-08-25 科德克希思公司 酮还原酶多肽及多核苷酸
SG11201909957TA (en) 2017-05-08 2019-11-28 Codexis Inc Engineered ligase variants
EP3638688A4 (de) 2017-06-14 2021-07-07 Codexis, Inc. Manipulierte transaminase-polypeptide für industrielle biokatalyse
JP7244089B2 (ja) 2017-06-27 2023-03-22 コデクシス， インコーポレイテッド ペニシリンｇアシラーゼ
CN111511389A (zh) 2017-11-07 2020-08-07 科德克希思公司 转谷氨酰胺酶变体
CA3081950A1 (en) 2017-12-13 2019-06-20 Codexis, Inc. Carboxyesterase polypeptides for amide coupling
WO2019241132A1 (en) 2018-06-12 2019-12-19 Codexis, Inc. Engineered tyrosine ammonia lyase
SG11202012136YA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered pantothenate kinase variant enzymes
KR20210031926A (ko) 2018-07-09 2021-03-23 코덱시스, 인코포레이티드 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체 효소
US11466259B2 (en) 2018-07-09 2022-10-11 Codexis, Inc. Engineered galactose oxidase variant enzymes
KR20210032417A (ko) 2018-07-09 2021-03-24 코덱시스, 인코포레이티드 조작된 데옥시리보스-포스페이트 알돌라아제
JP7493248B2 (ja) 2018-07-09 2024-05-31 コデクシス， インコーポレイテッド 操作されたホスホペントムターゼ改変体酵素
US11198861B2 (en) 2018-07-12 2021-12-14 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
MX2021001260A (es) 2018-07-30 2021-04-12 Tate & Lyle Solutions Usa Llc Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
KR20210084590A (ko) 2018-10-29 2021-07-07 코덱시스, 인코포레이티드 조작된 dna 중합효소 변이체
AU2019397401A1 (en) 2018-12-14 2021-06-17 Codexis, Inc. Engineered tyrosine ammonia lyase
JP2022518212A (ja) 2019-01-15 2022-03-14 コデクシス， インコーポレイテッド 操作されたトランスアミナーゼポリペプチド
JP7245676B2 (ja) * 2019-03-11 2023-03-24 花王株式会社 変異プロテアーゼ
US12215368B2 (en) 2019-05-01 2025-02-04 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
CN114096662B (zh) 2019-05-01 2025-04-15 科德克希思公司 工程化葡萄糖脱氢酶和用于酮和胺化合物的还原胺化的方法
CN114072165A (zh) 2019-07-02 2022-02-18 科德克希思公司 工程化蔗糖磷酸化酶变体酶
BR112021024819A2 (pt) 2019-07-02 2022-01-25 Codexis Inc Acetato quinase engenheirada, composição, sequência de polinucleotídeos, vetor de expressão, célula hospedeira, e, método de produção de uma acetato quinase engenheirada
MX2022002473A (es) 2019-08-30 2022-08-02 Codexis Inc Variantes de lipasa modificadas geneticamente.
JP2022547523A (ja) 2019-09-12 2022-11-14 コデクシス， インコーポレイテッド １０－アセチル－３，７－ジヒドロキシフェノキサジンに対するペルオキシダーゼ活性
US12344865B2 (en) 2019-11-26 2025-07-01 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
WO2021127457A1 (en) 2019-12-20 2021-06-24 Codexis, Inc. Engineered acid alpha-glucosidase variants
CN116113691A (zh) 2020-02-04 2023-05-12 科德克希思公司 工程化亮氨酸脱羧酶
IL296841A (en) 2020-04-10 2022-11-01 Codexis Inc Transaminase transaminase polypeptides
EP4204554A4 (de) 2020-08-28 2024-08-21 Codexis, Inc. Manipulierte proteasevarianten
AU2021331493A1 (en) 2020-08-28 2023-03-09 Codexis, Inc. Engineered amylase variants
AR124633A1 (es) * 2020-09-21 2023-04-19 Bayer Cropscience Lp Nuevas serina proteasas
EP4262804A4 (de) 2020-12-18 2025-02-12 Codexis, Inc. Manipulierte enzyme mit uridinphosphorylase-variante
CN117120600A (zh) 2021-04-02 2023-11-24 科德克希思公司 工程化腺苷酸激酶变体酶
CN117222735A (zh) 2021-04-02 2023-12-12 科德克希思公司 工程化乙酸激酶变体酶
CA3214973A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered guanylate kinase variant enzymes
IL305924B2 (en) 2021-04-02 2025-09-01 Codexis Inc Engineered Cyclic GMP-AMP Synthase (CGAS) Variant Enzymes
TW202313969A (zh) 2021-05-21 2023-04-01 美商克迪科思股份有限公司 經工程化的甲硫胺酸γ解離酶變異體
JP2024539636A (ja) 2021-10-15 2024-10-29 コデクシス， インコーポレイテッド 操作されたｄｎａポリメラーゼバリアント
US12565641B2 (en) 2021-10-15 2026-03-03 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
WO2023077169A2 (en) 2021-11-01 2023-05-04 Codexis, Inc. Engineered leucine decarboxylases
CA3257053A1 (en) 2022-05-14 2023-11-23 Novozymes A/S COMPOSITIONS AND METHODS FOR THE PREVENTION, TREATMENT, SUPPRESSION AND/OR ELIMINATION OF PHYTOPATHOGENIC INFESTATIONS AND INFECTIONS
WO2025235589A1 (en) 2024-05-08 2025-11-13 Codexis, Inc. Dna polymerase variants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US49827A (en) * 1865-09-05 Improvement in machines for making cement pipe
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
KR100258460B1 (ko) * 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
JP4028592B2 (ja) * 1992-07-17 2007-12-26 ジェネンコー インターナショナル インコーポレイテッド 高アルカリ性セリンプロテアーゼ
CA2145420C (en) * 1992-09-24 2005-01-04 Stanley E. Mainzer Cleaning compositions containing novel alkaline proteases
ATE245185T1 (de) * 1993-10-14 2003-08-15 Procter & Gamble Proteasen enthaltende bleichmittelzusammensetzungen
ES2287931T3 (es) * 1993-10-14 2007-12-16 THE PROCTER & GAMBLE COMPANY Composiciones limpiadoras que contienen proteasa.
US5972042A (en) 1995-12-22 1999-10-26 Novo Nordisk A/S Method for dyeing a material with a dyeing system which contains an enzymatic oxidizing agent
ATE409743T1 (de) * 1996-11-04 2008-10-15 Novozymes As Subtilase varianten und verbindungen
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162728A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Also Published As

Publication number Publication date
EP1287123B1 (de) 2011-03-02
US20100105599A1 (en) 2010-04-29
AU2006208427B2 (en) 2009-10-01
US20030049827A1 (en) 2003-03-13
AU2001249811B2 (en) 2006-06-08
AU2009251169A1 (en) 2010-01-21
AU4981101A (en) 2001-10-15
EP1287123A2 (de) 2003-03-05
JP2004508011A (ja) 2004-03-18
WO2001075087A8 (en) 2001-12-27
WO2001075087A3 (en) 2002-12-19
US7220566B2 (en) 2007-05-22
US6902922B2 (en) 2005-06-07
CA2405063C (en) 2013-06-04
US20040203130A1 (en) 2004-10-14
US7605115B2 (en) 2009-10-20
WO2001075087A2 (en) 2001-10-11
CA2405063A1 (en) 2001-10-11
AU2006208427A1 (en) 2006-09-28
DE60144145D1 (de) 2011-04-14
US20070270574A1 (en) 2007-11-22

Similar Documents

Publication Publication Date Title
DE60144145D1 (de) Subtilisin-variante
WO2002006457A3 (en) Novel lipase genes
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
DE60127441D1 (de) Dipeptidylpeptidasen
AR006188A1 (es) "composicion farmaceutica para inhibir la actividad de serinproteasa, metodo in vitro para inhibir la actividad de serinproteasa y metodo para preparar dicha composicion".
ATE380822T1 (de) Funf-helix protein
WO2002031134A3 (en) Novel serine protease genes related to dppiv
DE60134786D1 (de) Mini-dystrophin nukleinsäure- und peptidsequenzen
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
DK1349944T3 (da) Latensassocieret peptid af TGF-beta til at bibringe farmaceutisk aktive proteiner latens
ATE474926T1 (de) Verwendung von clya hemolysin fur ausscheidung von fusionprotein
ATE384787T1 (de) Tripeptidyl-aminopeptidase
WO2001025438A3 (en) Ifn-alpha homologues
DE60139544D1 (de) Veränderte, fluoreszierende proteine
DK1082423T3 (da) Cyclin E2-gener og -proteiner
WO2001064912A3 (en) Triazine degrading enzymes
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
ATE309370T1 (de) Retinoid-metabolisierendes protein
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
WO2002062964A3 (en) Ap1 amine oxidase variants
WO2001040257A3 (en) Modified il-12 subunits and nucleic acids encoding them
WO2001096593A3 (en) Novel motor proteins and methods for their use
WO2002012541A3 (en) Novel motor proteins and methods for their use
WO2005003289A8 (en) Ap1 amine oxidase variants
WO1999055885A3 (en) Novel serine protease capable of selective cleavage of insulin-like growth factor binding protein

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties