ATE500342T1 - Verfahren zur bestimmung von enzym aktivität - Google Patents

Verfahren zur bestimmung von enzym aktivität

Info

Publication number
ATE500342T1
ATE500342T1 AT03722751T AT03722751T ATE500342T1 AT E500342 T1 ATE500342 T1 AT E500342T1 AT 03722751 T AT03722751 T AT 03722751T AT 03722751 T AT03722751 T AT 03722751T AT E500342 T1 ATE500342 T1 AT E500342T1
Authority
AT
Austria
Prior art keywords
enzyme activity
determining enzyme
relates
measuring enzyme
determining
Prior art date
Application number
AT03722751T
Other languages
English (en)
Inventor
John Gerard Whateley
Original Assignee
Ge Healthcare Uk Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Uk Ltd filed Critical Ge Healthcare Uk Ltd
Application granted granted Critical
Publication of ATE500342T1 publication Critical patent/ATE500342T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT03722751T 2002-04-19 2003-04-17 Verfahren zur bestimmung von enzym aktivität ATE500342T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0208989.4A GB0208989D0 (en) 2002-04-19 2002-04-19 Methods for measuring enzyme activity
PCT/GB2003/001669 WO2003089663A2 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity

Publications (1)

Publication Number Publication Date
ATE500342T1 true ATE500342T1 (de) 2011-03-15

Family

ID=9935152

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03722751T ATE500342T1 (de) 2002-04-19 2003-04-17 Verfahren zur bestimmung von enzym aktivität

Country Status (9)

Country Link
US (2) US7727739B2 (de)
EP (1) EP1497452B1 (de)
JP (1) JP4713835B2 (de)
AT (1) ATE500342T1 (de)
AU (1) AU2003229914A1 (de)
CA (1) CA2484467C (de)
DE (1) DE60336217D1 (de)
GB (2) GB0208989D0 (de)
WO (1) WO2003089663A2 (de)

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JP4637565B2 (ja) * 2004-11-02 2011-02-23 一般社団法人オンチップ・セロミクス・コンソーシアム 生体試料標識物および生体物質標識法および生体物質の検査法
US7741431B2 (en) * 2005-02-01 2010-06-22 The United States Of America As Represented By The Secretary Of The Army Liposomes containing novel targeting and/or fusogenic peptides, preparations containing them and therapeutic use thereof
US20090220587A1 (en) * 2005-02-01 2009-09-03 United State Army Liposomal drug delivery constructs targeted by lipid-conjugated peptide ligands
EP1900822B1 (de) * 2005-05-24 2016-02-03 Suzuki, Miho Verfahren zur gleichzeitigen analyse mehrerer biologischer reaktionen oder veränderungen unter in-vivo-bedingungen
CA2627628A1 (en) * 2005-10-28 2007-05-03 Iti Scotland Limited Novel fluorescent dyes and uses thereof
US8691939B2 (en) 2007-06-08 2014-04-08 General Electric Company Compositions, methods, and kits for assaying complement activation
US20110039289A1 (en) * 2008-03-14 2011-02-17 Pierre Graves Fluorogenic peptides and their method of production
JP2010215572A (ja) * 2009-03-17 2010-09-30 Japan Health Science Foundation 歯髄炎診断マーカー及び歯髄炎診断システム
JP5697129B2 (ja) * 2010-03-24 2015-04-08 国立大学法人埼玉大学 Ｆｒｅｔを利用した酵素活性測定基質及びその製造方法
WO2012110833A2 (en) 2011-02-18 2012-08-23 Almac Sciences (Scotland) Limited Fluorescence modulators
US9180209B2 (en) 2011-02-28 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
WO2012131345A2 (en) 2011-03-25 2012-10-04 Almac Sciences (Scotland) Limited Enzyme assays
CN103765215A (zh) * 2011-06-07 2014-04-30 生命科技公司 发荧光半导体纳米晶体
KR101372632B1 (ko) 2011-08-26 2014-03-14 동국대학교 산학협력단 Ｘｐｇ 엔도뉴클레아제 활성 분석 방법
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes
US9229005B2 (en) 2012-03-23 2016-01-05 Surmodics Ivd, Inc. Compositions and methods for in vitro diagnostic tests including sulfonic acid compound
RU2622753C1 (ru) * 2016-11-17 2017-06-19 Федеральное государственное бюджетное учреждение "Национальный научно-практический центр сердечно-сосудистой хирургии имени А.Н. Бакулева" Министерства здравоохранения Российской Федерации (ФГБУ "ННПЦССХ им. А.Н. Бакулева" Минздрава России) Способ определения активности ангиотензин-превращающего фермента в тканях сердца
CN114605405B (zh) * 2022-03-09 2023-09-05 吉林大学 一种基于喹吖啶酮骨架的细胞脂滴荧光成像探针及其应用

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US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
FR2635114A1 (fr) * 1988-08-02 1990-02-09 Cibevial Sa Procede de traitement de solutions proteiques contenant des pigments tels que groupements heminiques ou chlorophylles en vue de leur decoloration et produits obtenus
EP0428000A1 (de) * 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
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Also Published As

Publication number Publication date
AU2003229914A1 (en) 2003-11-03
GB2388190A (en) 2003-11-05
WO2003089663A2 (en) 2003-10-30
JP2005527212A (ja) 2005-09-15
EP1497452B1 (de) 2011-03-02
DE60336217D1 (de) 2011-04-14
GB0208989D0 (en) 2002-05-29
GB0308910D0 (en) 2003-05-21
EP1497452A2 (de) 2005-01-19
CA2484467C (en) 2012-01-10
CA2484467A1 (en) 2003-10-30
GB2388190B (en) 2004-11-17
WO2003089663A3 (en) 2004-05-27
US20030228609A1 (en) 2003-12-11
JP4713835B2 (ja) 2011-06-29
US7727739B2 (en) 2010-06-01
US20100184098A1 (en) 2010-07-22

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