ATE520028T1 - Test zum nachweis von proteinbindung - Google Patents

Test zum nachweis von proteinbindung

Info

Publication number
ATE520028T1
ATE520028T1 AT04776903T AT04776903T ATE520028T1 AT E520028 T1 ATE520028 T1 AT E520028T1 AT 04776903 T AT04776903 T AT 04776903T AT 04776903 T AT04776903 T AT 04776903T AT E520028 T1 ATE520028 T1 AT E520028T1
Authority
AT
Austria
Prior art keywords
test
phage
protein binding
detecting protein
displayed
Prior art date
Application number
AT04776903T
Other languages
English (en)
Inventor
David Lockhart
Patrick Zarrinkar
Daniel Treiber
Original Assignee
Discoverx Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Discoverx Corp filed Critical Discoverx Corp
Application granted granted Critical
Publication of ATE520028T1 publication Critical patent/ATE520028T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
AT04776903T 2003-06-20 2004-06-21 Test zum nachweis von proteinbindung ATE520028T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48058703P 2003-06-20 2003-06-20
PCT/US2004/019943 WO2004113556A2 (en) 2003-06-20 2004-06-21 Assay and kits for detecting protein binding

Publications (1)

Publication Number Publication Date
ATE520028T1 true ATE520028T1 (de) 2011-08-15

Family

ID=33539315

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04776903T ATE520028T1 (de) 2003-06-20 2004-06-21 Test zum nachweis von proteinbindung

Country Status (8)

Country Link
US (2) US20050009099A1 (de)
EP (1) EP1644513B1 (de)
JP (2) JP4991291B2 (de)
AT (1) ATE520028T1 (de)
AU (1) AU2004250256B8 (de)
CA (1) CA2526847C (de)
DK (1) DK1644513T3 (de)
WO (1) WO2004113556A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1891446T3 (pl) * 2005-06-14 2013-08-30 Cellzome Gmbh Sposób identyfikacji nowych związków oddziałujących z enzymami
WO2007044894A2 (en) * 2005-10-11 2007-04-19 Chembridge Research Laboratories, Inc. Cell-free protein expression systems and methods of use thereof
JP5415264B2 (ja) 2006-06-30 2014-02-12 ドイスコベルク コーポレーション 検出可能な核酸タグ
ES2348187T3 (es) 2007-10-05 2010-12-01 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Amplificacion proteomica de pequeñas moleculas que se unen a proteinas celulares diana.
KR101028875B1 (ko) 2010-02-12 2011-04-12 메디스커브 주식회사 세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법
US20140243239A1 (en) * 2011-10-12 2014-08-28 University Of North Carolina At Chapel Hill Multiplexed kinase inhibitor beads and uses thereof
US9977015B2 (en) * 2012-05-15 2018-05-22 Massachusetts Institute Of Technology Systems and methods for detecting molecular interactions using magnetic beads
CN105593240B (zh) * 2013-06-11 2024-05-31 保罗·谢勒学院 用于以单氨基酸分辨率确定可突变配体-gpcr结合的方法以及突变配体和gpcr对
EP3183580B1 (de) 2014-08-21 2025-03-26 Eurofins DiscoverX Corporation Homogener thermal-shift-ligandenbindungstest
DE102015003503A1 (de) 2015-03-20 2016-09-22 Forschungszentrum Jülich GmbH Spezifisch Amyloid-Beta bindende Peptide und deren Verwendung für die Therapie und Diagnose der Alzheimerschen Demenz
WO2024020328A1 (en) * 2022-07-16 2024-01-25 Eurofins Discoverx Corporation Binding assays using phage display technology and kits thereof

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US5693489A (en) * 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5198346A (en) * 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
US5498538A (en) * 1990-02-15 1996-03-12 The University Of North Carolina At Chapel Hill Totally synthetic affinity reagents
US5437981A (en) * 1990-02-26 1995-08-01 Boehringer Mannheim Gmbh Method for the immunological determination of ligands
US6172197B1 (en) * 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
PT1024191E (pt) * 1991-12-02 2008-12-22 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos
ATE199392T1 (de) * 1992-12-04 2001-03-15 Medical Res Council Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung
AT401526B (de) * 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
US5536814A (en) * 1993-09-27 1996-07-16 La Jolla Cancer Research Foundation Integrin-binding peptides
JPH10511843A (ja) * 1994-07-08 1998-11-17 ニュー イングランド メディカル センター ホスピタルズ インコーポレイテッド Ｅ６結合タンパク質
US6326155B1 (en) * 1995-03-20 2001-12-04 Dyax Corp. Engineering affinity ligands for macromolecules
KR19990022651A (ko) 1995-06-07 1999-03-25 데이비드 엘. 버스테인 생물학적 사건에 대한 라파마이신 기재 조절방법
EP1017980A4 (de) * 1996-03-12 2002-09-18 Smithkline Beecham Corp Verfahren zur identifizierung pharmazeutisch aktiver verbindungen
US5766905A (en) * 1996-06-14 1998-06-16 Associated Universities Inc. Cytoplasmic bacteriophage display system
IT1291913B1 (it) * 1997-05-22 1999-01-21 Angeletti P Ist Richerche Bio Metodo che prevede l'uso di batteriofagi per la rivelazione della presenza di molecole di interesse in campioni biologici
US6245760B1 (en) * 1997-05-28 2001-06-12 Aventis Pharmaceuticals Products, Inc Quinoline and quinoxaline compounds which inhibit platelet-derived growth factor and/or p56lck tyrosine kinases
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US6897019B1 (en) * 1998-04-17 2005-05-24 Tufts College Methods for treating and preventing insulin resistance and related disorders
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JP2003508078A (ja) * 1999-09-03 2003-03-04 イエール ユニバーシティー 低分子プローブを使用して遺伝子機能を同定する方法
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US7060506B2 (en) * 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
JP2003522183A (ja) * 2000-02-02 2003-07-22 トランジェーヌ、ソシエテ、アノニム ターゲッティングペプチド
US6630335B1 (en) * 2000-02-11 2003-10-07 Millennium Pharmaceuticals, Inc. 14171 protein kinase, a novel human protein kinase and uses thereof
AU2001265717A1 (en) * 2000-06-20 2002-01-02 Euroscreen S.A. A recombinant cell line expressing gpcrx11 as a functional receptor validated byangiopeptin and useful for screening of agonists and antagonists
US20020123071A1 (en) * 2000-12-04 2002-09-05 Knudsen Sanne Moller Method of identifying compounds capable of acting as agonists or antagonists of G-protein coupled receptors
US7611711B2 (en) * 2001-01-17 2009-11-03 Vegenics Limited VEGFR-3 inhibitor materials and methods
AU2002317013B2 (en) * 2001-06-27 2007-11-01 Janssen Pharmaceutica N.V. EPF receptor assays, compounds and therapeutic compositions
CN1311084C (zh) * 2001-12-06 2007-04-18 阿隆戴蒙德艾滋病研究中心 利用嵌合噬菌体标准物的高灵敏基因组检测
US20030194749A1 (en) * 2002-02-15 2003-10-16 Wandless Thomas J. Wortmannin derivatives as probes of cellular proteins and processes

Also Published As

Publication number Publication date
JP2007524388A (ja) 2007-08-30
AU2004250256A1 (en) 2004-12-29
WO2004113556A3 (en) 2005-11-03
US9267165B2 (en) 2016-02-23
EP1644513A2 (de) 2006-04-12
JP2012044996A (ja) 2012-03-08
US20080058219A1 (en) 2008-03-06
WO2004113556A2 (en) 2004-12-29
DK1644513T3 (da) 2011-10-17
US20050009099A1 (en) 2005-01-13
EP1644513B1 (de) 2011-08-10
AU2004250256B2 (en) 2010-03-25
AU2004250256B8 (en) 2010-04-08
CA2526847C (en) 2011-01-25
EP1644513A4 (de) 2007-10-17
WO2004113556A8 (en) 2005-05-12
JP4991291B2 (ja) 2012-08-01
CA2526847A1 (en) 2004-12-29
JP5441978B2 (ja) 2014-03-12

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