AU6094794A - Non-denaturing potency assay for bovine somatotropin - Google Patents

Non-denaturing potency assay for bovine somatotropin

Info

Publication number: AU6094794A
Authority: AU; Australia
Prior art keywords: bst; bovine somatotropin; polymer gel; porous polymer; size exclusion
Prior art date: 1993-01-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU60947/94A

Other languages

English (en)

Inventor

Jen P Chang

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Eli Lilly and Co

Original Assignee

Eli Lilly and Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-01-26

Filing date

1994-01-24

Publication date

1994-08-15

1994-01-24 Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co

1994-08-15 Publication of AU6094794A publication Critical patent/AU6094794A/en

Status Abandoned legal-status Critical Current

Links

108010006025 bovine growth hormone Proteins 0.000 title claims description 209
238000003556 assay Methods 0.000 title description 14
238000000034 method Methods 0.000 claims description 71
238000003998 size exclusion chromatography high performance liquid chromatography Methods 0.000 claims description 59
102000004169 proteins and genes Human genes 0.000 claims description 48
108090000623 proteins and genes Proteins 0.000 claims description 48
239000000243 solution Substances 0.000 claims description 24
229920000642 polymer Polymers 0.000 claims description 18
ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 15
229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 15
235000012538 ammonium bicarbonate Nutrition 0.000 claims description 15
239000001099 ammonium carbonate Substances 0.000 claims description 15
239000002245 particle Substances 0.000 claims description 14
239000007853 buffer solution Substances 0.000 claims description 13
239000007864 aqueous solution Substances 0.000 claims description 10
239000011148 porous material Substances 0.000 claims description 9
230000005526 G1 to G0 transition Effects 0.000 claims description 4
108010051696 Growth Hormone Proteins 0.000 claims description 3
102000018997 Growth Hormone Human genes 0.000 claims description 3
238000011088 calibration curve Methods 0.000 claims description 3
241000283690 Bos taurus Species 0.000 claims description 2
235000018102 proteins Nutrition 0.000 description 44
239000012071 phase Substances 0.000 description 30
239000000523 sample Substances 0.000 description 16
230000000694 effects Effects 0.000 description 10
239000000499 gel Substances 0.000 description 10
238000010828 elution Methods 0.000 description 9
238000000926 separation method Methods 0.000 description 9
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 6
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
239000012062 aqueous buffer Substances 0.000 description 6
239000000463 material Substances 0.000 description 6
230000004044 response Effects 0.000 description 6
239000007790 solid phase Substances 0.000 description 5
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
230000004071 biological effect Effects 0.000 description 4
239000004202 carbamide Substances 0.000 description 4
230000007717 exclusion Effects 0.000 description 4
239000000126 substance Substances 0.000 description 4
241000700159 Rattus Species 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
230000008859 change Effects 0.000 description 3
239000003153 chemical reaction reagent Substances 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
238000004128 high performance liquid chromatography Methods 0.000 description 3
210000003000 inclusion body Anatomy 0.000 description 3
238000012986 modification Methods 0.000 description 3
230000004048 modification Effects 0.000 description 3
239000000178 monomer Substances 0.000 description 3
238000011084 recovery Methods 0.000 description 3
238000004007 reversed phase HPLC Methods 0.000 description 3
238000005185 salting out Methods 0.000 description 3
239000012488 sample solution Substances 0.000 description 3
230000004584 weight gain Effects 0.000 description 3
235000019786 weight gain Nutrition 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
230000006399 behavior Effects 0.000 description 2
239000013590 bulk material Substances 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
238000012512 characterization method Methods 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
239000003398 denaturant Substances 0.000 description 2
230000003292 diminished effect Effects 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
239000008187 granular material Substances 0.000 description 2
239000000122 growth hormone Substances 0.000 description 2
238000002347 injection Methods 0.000 description 2
239000007924 injection Substances 0.000 description 2
238000012417 linear regression Methods 0.000 description 2
230000014759 maintenance of location Effects 0.000 description 2
244000005700 microbiome Species 0.000 description 2
239000002244 precipitate Substances 0.000 description 2
230000008569 process Effects 0.000 description 2
238000000746 purification Methods 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
229950002069 somidobove Drugs 0.000 description 2
238000012360 testing method Methods 0.000 description 2
238000002211 ultraviolet spectrum Methods 0.000 description 2
239000003643 water by type Substances 0.000 description 2
241000894006 Bacteria Species 0.000 description 1
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
206010062767 Hypophysitis Diseases 0.000 description 1
239000012901 Milli-Q water Substances 0.000 description 1
239000004721 Polyphenylene oxide Substances 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000002776 aggregation Effects 0.000 description 1
238000004220 aggregation Methods 0.000 description 1
230000004075 alteration Effects 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
-1 aromatic amino acids Chemical class 0.000 description 1
239000000872 buffer Substances 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical class OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000000084 colloidal system Substances 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
210000000805 cytoplasm Anatomy 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
239000003599 detergent Substances 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
230000000762 glandular Effects 0.000 description 1
239000010931 gold Substances 0.000 description 1
229910052737 gold Inorganic materials 0.000 description 1
230000005484 gravity Effects 0.000 description 1
239000012535 impurity Substances 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
210000003635 pituitary gland Anatomy 0.000 description 1
229920000570 polyether Polymers 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
235000004252 protein component Nutrition 0.000 description 1
238000003908 quality control method Methods 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
239000002904 solvent Substances 0.000 description 1
108010057234 sometribove Proteins 0.000 description 1
229960000364 sometribove Drugs 0.000 description 1
230000003595 spectral effect Effects 0.000 description 1
239000012086 standard solution Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1
239000011800 void material Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
- B01J20/267—Cross-linked polymers
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28004—Sorbent size or size distribution, e.g. particle size
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/285—Porous sorbents based on polymers
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N2030/524—Physical parameters structural properties
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Analytical Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Endocrinology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Zoology (AREA)
Toxicology (AREA)
General Physics & Mathematics (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Pathology (AREA)
Dispersion Chemistry (AREA)
Peptides Or Proteins (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

AU60947/94A 1993-01-26 1994-01-24 Non-denaturing potency assay for bovine somatotropin Abandoned AU6094794A (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US903493A	1993-01-26	1993-01-26
US009034		1993-01-26
PCT/US1994/000850 WO1994017403A1 (en)	1993-01-26	1994-01-24	Non-denaturing potency assay for bovine somatotropin

Publications (1)

Publication Number	Publication Date
AU6094794A true AU6094794A (en)	1994-08-15

Family

ID=21735207

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AU60947/94A Abandoned AU6094794A (en)	1993-01-26	1994-01-24	Non-denaturing potency assay for bovine somatotropin

Country Status (13)

Country	Link
EP (1)	EP0681696A1 (da)
JP (1)	JPH08509288A (da)
KR (1)	KR960700454A (da)
CN (1)	CN1119042A (da)
AU (1)	AU6094794A (da)
BR (1)	BR9405672A (da)
CA (1)	CA2154794A1 (da)
CZ (1)	CZ193595A3 (da)
HU (1)	HUT75678A (da)
NO (1)	NO952948L (da)
PL (1)	PL310053A1 (da)
WO (1)	WO1994017403A1 (da)
ZA (1)	ZA94169B (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5567620A (en) *	1993-01-26	1996-10-22	Eli Lilly And Company	Non-denaturing potency assay for somatotropin
CN120685920A (zh) *	2025-06-20	2025-09-23	浙江毓昌生物技术有限公司	一种牛生长激素体外相对生物活性检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4511502A (en) *	1982-12-22	1985-04-16	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
BG49718A3 (bg) *	1983-07-15	1992-01-15	Bio- Technology General Corp	Метод за получаване на полипептид със супероксиддисмутазна активност
US5079230A (en) *	1988-09-12	1992-01-07	Pitman-Moore, Inc.	Stable bioactive somatotropins
US5064943A (en) *	1988-12-16	1991-11-12	American Cyanamid Company	Method for solubilization and naturation of somatotropin
US4975529A (en) *	1989-08-18	1990-12-04	Monsanto Company	Method of folding somatotropins
US5047511A (en) *	1989-08-28	1991-09-10	Pitman-Moore, Inc.	Method for recovering recombinant proteins

1994
- 1994-01-11 ZA ZA94169A patent/ZA94169B/xx unknown
- 1994-01-24 BR BR9405672A patent/BR9405672A/pt not_active Application Discontinuation
- 1994-01-24 CN CN94191402A patent/CN1119042A/zh active Pending
- 1994-01-24 AU AU60947/94A patent/AU6094794A/en not_active Abandoned
- 1994-01-24 EP EP94907307A patent/EP0681696A1/en not_active Withdrawn
- 1994-01-24 JP JP6517256A patent/JPH08509288A/ja active Pending
- 1994-01-24 PL PL94310053A patent/PL310053A1/xx unknown
- 1994-01-24 HU HU9502233A patent/HUT75678A/hu unknown
- 1994-01-24 CZ CZ951935A patent/CZ193595A3/cs unknown
- 1994-01-24 WO PCT/US1994/000850 patent/WO1994017403A1/en not_active Ceased
- 1994-01-24 CA CA002154794A patent/CA2154794A1/en not_active Abandoned
- 1994-01-24 KR KR1019950703075A patent/KR960700454A/ko not_active Withdrawn
1995
- 1995-07-25 NO NO952948A patent/NO952948L/no unknown

Also Published As

Publication number	Publication date
HU9502233D0 (en)	1995-09-28
CN1119042A (zh)	1996-03-20
JPH08509288A (ja)	1996-10-01
CA2154794A1 (en)	1994-01-24
NO952948D0 (no)	1995-07-25
NO952948L (no)	1995-09-18
PL310053A1 (en)	1995-11-13
HUT75678A (en)	1997-05-28
EP0681696A4 (da)	1995-11-22
EP0681696A1 (en)	1995-11-15
KR960700454A (ko)	1996-01-20
CZ193595A3 (en)	1996-05-15
WO1994017403A1 (en)	1994-08-04
BR9405672A (pt)	1995-11-14
ZA94169B (en)	1994-09-07

Publication	Publication Date	Title
Krummen et al.	1977	The separation of nonapeptides by reversed-phase high-performance liquid chromatography
Staub et al.	2011	Intact protein analysis in the biopharmaceutical field
US4377482A (en)	1983-03-22	Mobile phase for liquid chromatography
Takeuchi et al.	1986	Enantiomeric resolution of dansyl amino acids by micro high-performance liquid chromatography with β-cyclodextrin inclusion complexes
Grinberg et al.	1989	Protein aggregation in high-performance liquid chromatography: hydrophobic interaction chromatography of. beta.-lactoglobulin A
EP0206792B1 (en)	1991-11-06	High performance liquid chromatography mobile phase
Elrod Jr et al.	1992	Determination of benzalkonium chloride in eye care products by high-performance liquid chromatography and solid-phase extraction or on-line column switching
AU6094794A (en)	1994-08-15	Non-denaturing potency assay for bovine somatotropin
Thévenon et al.	1989	Reversed-phase liquid chromatography of proteins with strong acids
US5567620A (en)	1996-10-22	Non-denaturing potency assay for somatotropin
Lee et al.	1991	Influence of the mobile phase on salmon calcitonin analysis by reversed-phase liquid chromatography
Stuting et al.	1991	Determination of pituitary and recombinant human growth hormone molecular weights by modern high-performance liquid chromatography with low angle laser light scattering detection
Yang et al.	1987	High-speed and high-performance size-exclusion chromatography of proteins on a new hydrophilic polystyrene-based resin
Luo et al.	2011	Preparation of monolithic imprinted stationary phase for clenbuterol by in situ polymerization and application in biological samples pretreatment
Guitart et al.	1991	Stability of phenyl (thiocarbamoyl) amino acids and optimization of their separation by high-performance liquid chromatography
Goheen	1988	The influence of pH and acetonitrile on the high performance size exclusion profile of proteins
Bishop et al.	1981	The Preparative Separation of Synthetic Peptides on Reversed-Phase Silica Packed in Radially Compressed Flexible-Walled Columns.+
Szabo et al.	1988	High-performance liquid chromatography of proteins on stabilized polymer-diol-bonded silica-gel stationary phase
Compton et al.	1994	Chromatographic analysis of therapeutic proteins
Lombardi et al.	1992	Reverse phase high performance liquid chromatographic fractionation of marine organic matter
Chang et al.	1994	Non-denaturing assay for the determination of the potency of recombinant bovine somatotropin by high-performance size-exclusion chromatography
Kelly et al.	1989	Analysis of basic drugs by high-performance liquid chromatography using on-line solid-phase extraction and an unmodified silica column
Wilson et al.	1981	High-Performance Liquid Chromatography of Peptides and Proteins. Pharmaceutical and Bio-medical Applications
Welinder et al.	1983	Binding affinity of monoiodinated insulin tracers isolated after reversed-phase high-performance liquid chromatography
CN113358763B (zh)	2022-07-19	一种高效液相色谱测定十二烷基肌氨酸钠含量的方法