JPH08509288A - ウシソマトトロピンの未変性力価アッセイ - Google Patents

ウシソマトトロピンの未変性力価アッセイ

Info

Publication number
JPH08509288A
JPH08509288A JP6517256A JP51725694A JPH08509288A JP H08509288 A JPH08509288 A JP H08509288A JP 6517256 A JP6517256 A JP 6517256A JP 51725694 A JP51725694 A JP 51725694A JP H08509288 A JPH08509288 A JP H08509288A
Authority
JP
Japan
Prior art keywords
bst
bovine somatotropin
porous polymer
hydrophilic porous
polymer gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP6517256A
Other languages
English (en)
Japanese (ja)
Inventor
チャン、イェン・ピー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of JPH08509288A publication Critical patent/JPH08509288A/ja
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B01J20/267—Cross-linked polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28004—Sorbent size or size distribution, e.g. particle size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/291—Gel sorbents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP6517256A 1993-01-26 1994-01-24 ウシソマトトロピンの未変性力価アッセイ Pending JPH08509288A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US903493A 1993-01-26 1993-01-26
US08/009,034 1993-01-26
PCT/US1994/000850 WO1994017403A1 (en) 1993-01-26 1994-01-24 Non-denaturing potency assay for bovine somatotropin

Publications (1)

Publication Number Publication Date
JPH08509288A true JPH08509288A (ja) 1996-10-01

Family

ID=21735207

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6517256A Pending JPH08509288A (ja) 1993-01-26 1994-01-24 ウシソマトトロピンの未変性力価アッセイ

Country Status (13)

Country Link
EP (1) EP0681696A1 (da)
JP (1) JPH08509288A (da)
KR (1) KR960700454A (da)
CN (1) CN1119042A (da)
AU (1) AU6094794A (da)
BR (1) BR9405672A (da)
CA (1) CA2154794A1 (da)
CZ (1) CZ193595A3 (da)
HU (1) HUT75678A (da)
NO (1) NO952948L (da)
PL (1) PL310053A1 (da)
WO (1) WO1994017403A1 (da)
ZA (1) ZA94169B (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5567620A (en) * 1993-01-26 1996-10-22 Eli Lilly And Company Non-denaturing potency assay for somatotropin
CN120685920A (zh) * 2025-06-20 2025-09-23 浙江毓昌生物技术有限公司 一种牛生长激素体外相对生物活性检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
US4975529A (en) * 1989-08-18 1990-12-04 Monsanto Company Method of folding somatotropins
US5047511A (en) * 1989-08-28 1991-09-10 Pitman-Moore, Inc. Method for recovering recombinant proteins

Also Published As

Publication number Publication date
HU9502233D0 (en) 1995-09-28
CN1119042A (zh) 1996-03-20
CA2154794A1 (en) 1994-01-24
NO952948D0 (no) 1995-07-25
NO952948L (no) 1995-09-18
PL310053A1 (en) 1995-11-13
HUT75678A (en) 1997-05-28
EP0681696A4 (da) 1995-11-22
AU6094794A (en) 1994-08-15
EP0681696A1 (en) 1995-11-15
KR960700454A (ko) 1996-01-20
CZ193595A3 (en) 1996-05-15
WO1994017403A1 (en) 1994-08-04
BR9405672A (pt) 1995-11-14
ZA94169B (en) 1994-09-07

Similar Documents

Publication Publication Date Title
Krummen et al. The separation of nonapeptides by reversed-phase high-performance liquid chromatography
Tarr et al. Reverse-phase high-performance liquid chromatography of hydrophobic proteins and fragments thereof
Einarsson Selective determination of secondary amino acids using precolumn derivatization with 9-fluorenylmethylchloroformate and reversed-phase high-performance liquid chromatography
Ward et al. Chromatography of luteinizing hormone from sheep pituitary glands
JP3894570B2 (ja) Ｉｇｆ−ｉの精製方法
JP2001526960A (ja) 自由溶液リガンド相互作用分子分離方法
Simpson et al. A high‐performance liquid chromatography procedure for recovering subnanomole amounts of protein from SDS‐gel electroeluates for gas‐phase sequence analysis
Bietz High performance liquid chromatography: How proteins look in cereals
EP0206792B1 (en) High performance liquid chromatography mobile phase
JPH08509288A (ja) ウシソマトトロピンの未変性力価アッセイ
Thévenon et al. Reversed-phase liquid chromatography of proteins with strong acids
AU757858B2 (en) Process for the separation of glycosylated and non-glycosylated proteins
JP2001228133A (ja) ヘモグロビン類の測定方法
Lee et al. Influence of the mobile phase on salmon calcitonin analysis by reversed-phase liquid chromatography
US5567620A (en) Non-denaturing potency assay for somatotropin
Steckerf et al. Preparative chromatography and analysis of molecular weight fractions of gelatin
JP2001021555A (ja) 溶血試薬及びこれを用いたヘモグロビン類の測定方法
Hacha et al. Preparation and Some Properties of Strongly Acidic Proteins from Calf‐Thymus Nucleohistone
Kelly et al. Analysis of basic drugs by high-performance liquid chromatography using on-line solid-phase extraction and an unmodified silica column
Szuchet et al. Dissociation of myosin in acid
Hanis et al. Separation of elastin cross-links as phenylisothiocyanate derivatives
CA1288692C (en) Interferon assay
US3308027A (en) Bovine pituitary growth hormone process
EP0291988A2 (de) IL-2 Reinigung mittels Rezeptor-Affinitäts-Chromatographie
Kamp High performance liquid chromatography of proteins