BR0012963A - Engenharia metabólica papa a produção de aminoácidos - Google Patents

Engenharia metabólica papa a produção de aminoácidos

Info

Publication number
BR0012963A
BR0012963A BR0012963-1A BR0012963A BR0012963A BR 0012963 A BR0012963 A BR 0012963A BR 0012963 A BR0012963 A BR 0012963A BR 0012963 A BR0012963 A BR 0012963A
Authority
BR
Brazil
Prior art keywords
amino acids
production
mutagenesis
microorganisms
amino acid
Prior art date
Application number
BR0012963-1A
Other languages
English (en)
Other versions
BR0012963B1 (pt
Inventor
John P Rayapati
Corey M Crafton
Original Assignee
Archer Daniels Midland Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Archer Daniels Midland Co filed Critical Archer Daniels Midland Co
Publication of BR0012963A publication Critical patent/BR0012963A/pt
Publication of BR0012963B1 publication Critical patent/BR0012963B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"ENGENHARIA METABóLICA PARA A PRODUçãO DE AMINOáCIDOS". A presente invenção é dirigida para a produção fermentativa de aminoácidos, provendo microorganismos, métodos e processos utilizáveis. Os microorganismos da invenção são capazes de converter glucose em aminoácidos diferentes de L-isoleucina, L-leucina e L-valina com maior eficiência do que a cepa parental. A invenção provê microorganismos que são tornados auxotróficos ou braditróficos para a síntese de um ou mais aminoácidos de cadeia ramificada por mutagênese e selecionados por sua capacidade de produzir maiores rendimentos percentuais do aminoácido desejado do que a cepa parental. Os microorganismos preferidos são Corynebacterium, Brevibacterium ou Escherichia coli produzindo L-lisina. A mutagênese é realizada por técnicas clássicas ou através de metodologia de rDNA. Os métodos da invenção são projetados para aumentar a produção de um aminoácido por mutagênese de uma cepa parental, selecionando células auxotróficas ou braditróficas para a síntese de um ou mais aminoácidos de cadeia ramificada e selecionando auxotrofos ou braditrofos de aminoácido de cadeia ramificada que produzem um maior rendimento percentual a partir de dextrose do desejado aminoácido do que a cepa parental. Os processos da invenção são projetados para a produção de um aminoácido compreendendo cultivar, em um meio, um microorganismo obtido por mutagênese de uma cepa parental e selecionando variantes que são capazes de converter glucose em aminoácidos diferentes de Lisoleucina, L-leucina e L-valina com maior eficiência do que a cepa parental.
BRPI0012963-1B1A 1999-08-02 2000-08-02 engenharia metabàlica papa a produÇço de aminoÁcidos BR0012963B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14637999P 1999-08-02 1999-08-02
PCT/US2000/020979 WO2001009286A1 (en) 1999-08-02 2000-08-02 Metabolic engineering of amino acid production

Publications (2)

Publication Number Publication Date
BR0012963A true BR0012963A (pt) 2002-04-23
BR0012963B1 BR0012963B1 (pt) 2013-09-24

Family

ID=22517109

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0012963-1B1A BR0012963B1 (pt) 1999-08-02 2000-08-02 engenharia metabàlica papa a produÇço de aminoÁcidos

Country Status (12)

Country Link
US (2) US7323321B2 (pt)
EP (1) EP1200553B1 (pt)
JP (3) JP2003506030A (pt)
KR (1) KR100815041B1 (pt)
CN (1) CN1367823A (pt)
AU (1) AU779019B2 (pt)
BR (1) BR0012963B1 (pt)
CA (1) CA2380613C (pt)
DE (1) DE60042552D1 (pt)
MX (1) MXPA02001174A (pt)
NZ (1) NZ516893A (pt)
WO (1) WO2001009286A1 (pt)

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DE10063314A1 (de) * 2000-12-20 2002-07-04 Degussa Neue für das ilvE-Gen kodierende Nukleotidsequenzen
BR0304860A (pt) * 2002-11-11 2004-08-31 Ajinomoto Kk Método para produzir uma substância alvo pela utilização de uma-bactéria pertencente ao gênero escherichia
AR058584A1 (es) 2005-12-22 2008-02-13 Takeda Pharmaceutical Derivados de metastina y uso de los mismos
KR101368922B1 (ko) 2008-03-03 2014-02-27 글로벌 바이오-켐 테크놀로지 그룹 컴퍼니 리미티드 재조합 미생물 및 ｌ－리신을 생산하는 방법
DE102010003419B4 (de) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von L-Ornithin
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
JP6136930B2 (ja) * 2011-11-02 2017-05-31 味の素株式会社 タンパク質の分泌生産法
JP6020581B2 (ja) 2011-11-02 2016-11-02 味の素株式会社 タンパク質の分泌生産法
CN102604881B (zh) * 2012-03-31 2013-12-18 福建省麦丹生物集团有限公司 生产l-异亮氨酸的工程菌及其应用
KR101720836B1 (ko) 2014-08-05 2017-04-03 씨제이제일제당 (주) 피드백 저항성 아세토하이드록시산 신타아제 변이체 및 이를 이용한 l-발린의 생산방법
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2016133134A1 (ja) 2015-02-17 2016-08-25 味の素株式会社 有機化合物又は微生物の製造システム及び製造方法
KR101996129B1 (ko) * 2017-07-11 2019-07-04 씨제이제일제당 (주) 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법
US11021697B2 (en) 2017-07-11 2021-06-01 Cj Cheiljedang Corporation Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing L-branched-chain amino acid using the same
WO2019118548A1 (en) * 2017-12-15 2019-06-20 Archer Daniels Midland Company Engineered strains of corynebacteria
TWI716841B (zh) * 2018-03-27 2021-01-21 南韓商Ｃｊ第一製糖股份有限公司 新穎啟動子及使用該啟動子製造l-胺基酸的方法
JP2022500024A (ja) 2018-09-07 2022-01-04 アーチャー−ダニエルズ−ミッドランド カンパニー エンジニアリングされたコリネバクテリア（Ｃｏｒｙｎｅｂａｃｔｅｒｉａ）株
KR102147381B1 (ko) * 2019-11-22 2020-08-24 씨제이제일제당 주식회사 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물

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Also Published As

Publication number Publication date
KR100815041B1 (ko) 2008-03-18
EP1200553B1 (en) 2009-07-15
JP2003506030A (ja) 2003-02-18
JP2010022374A (ja) 2010-02-04
BR0012963B1 (pt) 2013-09-24
JP5319489B2 (ja) 2013-10-16
AU6617400A (en) 2001-02-19
CA2380613C (en) 2014-03-25
NZ516893A (en) 2003-02-28
US20060154345A1 (en) 2006-07-13
WO2001009286A1 (en) 2001-02-08
US7323321B2 (en) 2008-01-29
KR20020033749A (ko) 2002-05-07
CA2380613A1 (en) 2001-02-08
JP2006280383A (ja) 2006-10-19
AU779019B2 (en) 2005-01-06
DE60042552D1 (de) 2009-08-27
CN1367823A (zh) 2002-09-04
MXPA02001174A (es) 2002-07-30
US7635579B2 (en) 2009-12-22
US20080090272A1 (en) 2008-04-17
EP1200553A1 (en) 2002-05-02

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]

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B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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