BR0014059B1 - processo para produzir transglutaminase. - Google Patents
processo para produzir transglutaminase.Info
- Publication number
- BR0014059B1 BR0014059B1 BRPI0014059-7A BR0014059A BR0014059B1 BR 0014059 B1 BR0014059 B1 BR 0014059B1 BR 0014059 A BR0014059 A BR 0014059A BR 0014059 B1 BR0014059 B1 BR 0014059B1
- Authority
- BR
- Brazil
- Prior art keywords
- transglutaminase
- coryneform bacterium
- foreign protein
- pro
- secretory production
- Prior art date
Links
- 108060008539 Transglutaminase Proteins 0.000 title abstract 6
- 102000003601 transglutaminase Human genes 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 241000186254 coryneform bacterium Species 0.000 abstract 5
- 230000003248 secreting effect Effects 0.000 abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/104—Aminoacyltransferases (2.3.2)
- C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
- C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28009899 | 1999-09-30 | ||
| JP2000194043 | 2000-06-28 | ||
| PCT/JP2000/006780 WO2001023591A1 (en) | 1999-09-30 | 2000-09-29 | Process for producing transglutaminase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR0014059A BR0014059A (pt) | 2002-05-14 |
| BR0014059B1 true BR0014059B1 (pt) | 2013-02-05 |
Family
ID=26553616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0014059-7A BR0014059B1 (pt) | 1999-09-30 | 2000-09-29 | processo para produzir transglutaminase. |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7723067B2 (pt) |
| EP (1) | EP1219713B1 (pt) |
| JP (1) | JP4320769B2 (pt) |
| KR (1) | KR100565030B1 (pt) |
| CN (1) | CN100497632C (pt) |
| AT (1) | ATE532870T1 (pt) |
| AU (1) | AU7449400A (pt) |
| BR (1) | BR0014059B1 (pt) |
| CA (1) | CA2391961C (pt) |
| DK (1) | DK1219713T3 (pt) |
| ES (1) | ES2372806T3 (pt) |
| WO (1) | WO2001023591A1 (pt) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100575394B1 (ko) * | 2000-08-17 | 2006-05-03 | 아지노모토 가부시키가이샤 | 미생물 유래 트랜스글루타미나제의 변이 방법 |
| CN1250722C (zh) * | 2001-03-30 | 2006-04-12 | 味之素株式会社 | 分泌生产蛋白质的方法 |
| US7485438B2 (en) | 2002-03-01 | 2009-02-03 | Szu-Yi Chou | Method of producing polyvalent antigens |
| US7101695B2 (en) | 2002-03-01 | 2006-09-05 | Szu-Yi Chou | Method of producing transglutaminase having broad substrate activity |
| JP4323147B2 (ja) * | 2002-09-10 | 2009-09-02 | 天野エンザイム株式会社 | トランスグルタミナーゼ生産菌 |
| AU2003268693A1 (en) * | 2002-09-30 | 2004-04-19 | Ajinomoto Co., Inc. | Method of producing stable isotope-labeled protein |
| KR20050106502A (ko) | 2003-03-07 | 2005-11-09 | 아지노모토 가부시키가이샤 | 미생물 트랜스글루타미나제의 제조방법 |
| JP4730302B2 (ja) * | 2004-04-20 | 2011-07-20 | 味の素株式会社 | タンパク質の製造法 |
| US20090117640A1 (en) * | 2005-08-18 | 2009-05-07 | Novo Nordisk Health Care Ag | Transglutaminase Variants with Improved Specificity |
| WO2008020075A1 (en) * | 2006-08-18 | 2008-02-21 | Novo Nordisk Health Care Ag | Transglutaminase variants with improved specificity |
| AU2008215422B2 (en) | 2007-02-15 | 2011-07-14 | Ajinomoto Co., Inc. | Transglutaminase having disulfide bond introduced therein |
| US20100087371A1 (en) * | 2007-02-22 | 2010-04-08 | Sean Hu | Transglutaminase Variants with Improved Specificity |
| EP2138049B1 (en) | 2007-03-29 | 2014-06-25 | Ajinomoto Co., Inc. | Enzyme preparation for adhesion and method of producing adhesion-molded food product |
| KR20100085083A (ko) | 2007-11-19 | 2010-07-28 | 아지노모토 가부시키가이샤 | 섬유 가공물 및 이의 제조법 |
| EP2251421B1 (en) * | 2008-02-13 | 2016-11-16 | Amano Enzyme Inc. | Stabilized transglutaminase and process for production thereof |
| TWI444146B (zh) | 2008-09-25 | 2014-07-11 | Ajinomoto Kk | 黏著成型食品用酵素製劑及黏著成型食品之製造方法 |
| CN102341497B (zh) * | 2009-03-06 | 2013-11-06 | 味之素株式会社 | 放线菌来源的耐热性转谷氨酰胺酶 |
| US20100316764A1 (en) * | 2009-06-10 | 2010-12-16 | Engrain, LLC | Flour supplement compositions and methods for preparing wheat flour |
| EP2772546B1 (en) * | 2011-10-25 | 2019-05-15 | Ajinomoto Co., Inc. | Secretion production method for protein |
| JP6020581B2 (ja) | 2011-11-02 | 2016-11-02 | 味の素株式会社 | タンパク質の分泌生産法 |
| JP6136930B2 (ja) | 2011-11-02 | 2017-05-31 | 味の素株式会社 | タンパク質の分泌生産法 |
| CN102586167B (zh) * | 2012-03-01 | 2013-07-24 | 华南理工大学 | 一株重组的枯草芽孢杆菌及由其生产转谷氨酰胺酶的方法 |
| CN103060283B (zh) * | 2012-12-28 | 2014-05-28 | 天津科技大学 | 一种抑制枯草芽孢杆菌谷氨酰胺转胺酶活性的多肽及其筛选方法和应用 |
| EP2860259B1 (en) | 2013-02-18 | 2016-11-09 | Ajinomoto Co., Inc. | Method for producing protein by precipitation |
| CN103146661A (zh) * | 2013-03-07 | 2013-06-12 | 哈尔滨工业大学 | MgCl2胁迫提高茂原链霉菌谷氨酰胺转氨酶产量的方法 |
| RU2013119826A (ru) | 2013-04-29 | 2014-11-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Коринеформная бактерия и способ получения гетерологичных гибридных белков |
| DK3287523T3 (da) | 2015-04-24 | 2022-07-25 | Ajinomoto Kk | Fremgangsmåde til sekretorisk fremstilling af protein |
| EP3533875B1 (en) * | 2016-09-01 | 2023-02-15 | Ningxia Eppen Biotech Co. Ltd | Corynebacterium for producing l-lysine by fermentation |
| KR102335301B1 (ko) | 2016-10-21 | 2021-12-06 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
| KR102337932B1 (ko) | 2016-10-21 | 2021-12-13 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
| BR112019018859A2 (pt) | 2017-03-28 | 2020-04-14 | Ajinomoto Co., Inc. | método para produzir rna objetivo |
| WO2019078095A1 (en) | 2017-10-16 | 2019-04-25 | Ajinomoto Co., Inc. | PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY |
| CN107574159B (zh) * | 2017-10-26 | 2020-05-08 | 江南大学 | 一种以活性形式表达的谷氨酰胺转氨酶的突变体 |
| JP7367667B2 (ja) | 2018-02-20 | 2023-10-24 | 味の素株式会社 | Ｒｎａサイレンシングを誘導する方法 |
| KR102744950B1 (ko) | 2018-04-20 | 2024-12-20 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
| EP4118194A4 (en) * | 2020-03-13 | 2024-08-21 | Curie Co. Inc. | TRANSGLUTAMINASE VARIANTS |
| CN113528479B (zh) * | 2020-04-13 | 2023-01-24 | 中国科学院微生物研究所 | 一种转谷氨酰胺酶的高效制备方法及其专用工程菌 |
| EP4166647A4 (en) | 2020-06-11 | 2025-01-01 | Ajinomoto Co., Inc. | Method for producing protein |
| CN111944778B (zh) * | 2020-08-14 | 2022-06-21 | 安徽医学高等专科学校 | 谷氨酰胺转胺酶突变体及其编码基因和应用 |
| KR20240032941A (ko) | 2021-07-07 | 2024-03-12 | 아지노모토 가부시키가이샤 | 비천연 아미노산 함유 단백질의 분비 생산법 |
| JPWO2023200008A1 (pt) | 2022-04-15 | 2023-10-19 | ||
| US20250304927A1 (en) * | 2022-05-20 | 2025-10-02 | Curie Co. Inc. | Pro-peptide variants for modulating activity of transglutaminases |
| KR20250151452A (ko) | 2023-02-16 | 2025-10-21 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8800352A1 (es) * | 1985-04-05 | 1987-10-16 | Sankyo Co | Un procedimiento para producir un compuesto capaz de funcionar como una elastasa pancreatica humana |
| RU2091490C1 (ru) | 1985-10-25 | 1997-09-27 | Зимодженетикс, Инк. | Способ получения гетерологичного полипептида в эукариотических микроорганизмов |
| US4965194A (en) * | 1986-08-21 | 1990-10-23 | Toyo Boseki Kabushiki Kaisha | Pyruvate oxidase and an analytical method using the same |
| WO1988009821A1 (en) * | 1987-06-12 | 1988-12-15 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
| JPH05244947A (ja) * | 1992-03-04 | 1993-09-24 | Mercian Corp | 新規ポストプロリンエンドペプチダーゼおよびその製造方法 |
| JPH0736754B2 (ja) | 1993-07-16 | 1995-04-26 | 三共株式会社 | ヒト・膵臓エラスターゼｉｉｉｂ |
| JPH09316095A (ja) * | 1996-05-27 | 1997-12-09 | Mitsubishi Chem Corp | 新規シグナルペプチド |
| JP3711658B2 (ja) * | 1996-10-07 | 2005-11-02 | 味の素株式会社 | コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質 |
| JP3305609B2 (ja) | 1997-03-06 | 2002-07-24 | ダイハツ工業株式会社 | 車両用スペアタイヤの支持構造 |
| JPH10244947A (ja) | 1997-03-06 | 1998-09-14 | Toyoda Gosei Co Ltd | ステアリングホイール芯金 |
-
2000
- 2000-09-29 BR BRPI0014059-7A patent/BR0014059B1/pt active IP Right Grant
- 2000-09-29 AT AT00962986T patent/ATE532870T1/de active
- 2000-09-29 DK DK00962986.6T patent/DK1219713T3/da active
- 2000-09-29 KR KR1020027004159A patent/KR100565030B1/ko not_active Expired - Lifetime
- 2000-09-29 CA CA002391961A patent/CA2391961C/en not_active Expired - Lifetime
- 2000-09-29 EP EP00962986A patent/EP1219713B1/en not_active Expired - Lifetime
- 2000-09-29 CN CNB008137331A patent/CN100497632C/zh not_active Expired - Lifetime
- 2000-09-29 ES ES00962986T patent/ES2372806T3/es not_active Expired - Lifetime
- 2000-09-29 AU AU74494/00A patent/AU7449400A/en not_active Abandoned
- 2000-09-29 WO PCT/JP2000/006780 patent/WO2001023591A1/ja not_active Ceased
- 2000-09-29 JP JP2001526973A patent/JP4320769B2/ja not_active Expired - Lifetime
-
2002
- 2002-04-01 US US10/112,488 patent/US7723067B2/en not_active Expired - Fee Related
-
2010
- 2010-02-26 US US12/714,151 patent/US7972829B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US7723067B2 (en) | 2010-05-25 |
| EP1219713A4 (en) | 2005-02-02 |
| BR0014059A (pt) | 2002-05-14 |
| CN1377413A (zh) | 2002-10-30 |
| ATE532870T1 (de) | 2011-11-15 |
| EP1219713B1 (en) | 2011-11-09 |
| CA2391961C (en) | 2008-12-02 |
| EP1219713A1 (en) | 2002-07-03 |
| ES2372806T3 (es) | 2012-01-26 |
| US20100297729A1 (en) | 2010-11-25 |
| KR100565030B1 (ko) | 2006-03-30 |
| CN100497632C (zh) | 2009-06-10 |
| DK1219713T3 (da) | 2011-12-12 |
| US20030082746A1 (en) | 2003-05-01 |
| JP4320769B2 (ja) | 2009-08-26 |
| KR20020038787A (ko) | 2002-05-23 |
| AU7449400A (en) | 2001-04-30 |
| CA2391961A1 (en) | 2001-04-05 |
| US7972829B2 (en) | 2011-07-05 |
| WO2001023591A1 (en) | 2001-04-05 |
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Legal Events
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| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] |
Free format text: O DEPOSITANTE DEVERA APRESENTAR A PETICAO DEVIDA PARA O CUMPRIMENTO DESTA EXIGENCIA EM ATE 60 (SESSENTA) DIAS, CONTADOS A PARTIR DA DATA DE PUBLICACAO NA RPI, DE ACORDO COM O ART. 34 (II) DA LPI PARA QUE SE DE INICIO AO EXAME TECNICO DO PEDIDO. |
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| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 13 E 10 (IX) DA LPI. |
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Free format text: REFERENTE A RPI 2096 DE 09/03/2011. |
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| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B09X | Republication of the decision to grant [chapter 9.1.3 patent gazette] |
Free format text: O PRESENTE PEDIDO TEVE UM PARECER DE DEFERIMENTO NOTIFICADO NA RPI NO 2151 DE 27/03/2012, TENDO SIDO CONSTATADO QUE ESTA NOTIFICACAO FOI EFETUADA COM INCORRECOES (O PARECER DE DEFERIMENTO FOI PUBLICADO COM O TITULO INICIAL E NAO COM O TITULO FINAL MODIFICADO ATRAVES DA PETICAO NO 20100096356 DE 14/10/2010). ASSIM SENDO, REPUBLICO A REFERIDA PUBLICACAO. |
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| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
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