BR0016569A - Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina - Google Patents

Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina

Info

Publication number
BR0016569A
BR0016569A BR0016569-7A BR0016569A BR0016569A BR 0016569 A BR0016569 A BR 0016569A BR 0016569 A BR0016569 A BR 0016569A BR 0016569 A BR0016569 A BR 0016569A
Authority
BR
Brazil
Prior art keywords
formalin
rna isolation
fixed paraffin
tissue
tissue specimens
Prior art date
Application number
BR0016569-7A
Other languages
English (en)
Inventor
Kathleen Danenberg
Peter Danenberg
Steven Swenson
Original Assignee
Univ Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California filed Critical Univ Southern California
Publication of BR0016569A publication Critical patent/BR0016569A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"PROCESSO DE ISOLAMENTO DE RNA DE ESPéCIMES DE TECIDO EMBUTIDOS - PARAFINA FIXADOS - FORMALINA". São mostrados processos para isolamento rápido, simples e confiável de RNA de amostras de tecido embutidas em parafina fixadas com formalina. RNA purificado desta maneira pode ser usado para monitorar níveis de expressão de gene. A amostra de tecido pode ser um tecido de tumor ou outro tecido patológico.
BR0016569-7A 1999-12-20 2000-10-31 Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina BR0016569A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
PCT/US2000/030012 WO2001046402A1 (en) 1999-12-20 2000-10-31 Method for isolation of rna from formalin-fixed paraffin-embedded tissue specimens

Publications (1)

Publication Number Publication Date
BR0016569A true BR0016569A (pt) 2003-07-15

Family

ID=23863395

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0016569-7A BR0016569A (pt) 1999-12-20 2000-10-31 Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina

Country Status (19)

Country Link
US (8) US6248535B1 (pt)
EP (3) EP2363477A3 (pt)
JP (1) JP2003525033A (pt)
KR (1) KR100723328B1 (pt)
CN (2) CN1891836B (pt)
AR (1) AR027036A1 (pt)
AT (2) ATE349518T1 (pt)
AU (2) AU784565C (pt)
BR (1) BR0016569A (pt)
CA (1) CA2394618C (pt)
DE (1) DE60032634T2 (pt)
DK (1) DK1743939T3 (pt)
EA (2) EA008832B1 (pt)
ES (1) ES2373149T3 (pt)
IL (2) IL150116A0 (pt)
MX (1) MXPA02006145A (pt)
NO (1) NO330802B1 (pt)
TW (1) TWI277652B (pt)
WO (1) WO2001046402A1 (pt)

Families Citing this family (247)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6207408B1 (en) * 1997-08-20 2001-03-27 University Of Miami High quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method
US6793890B2 (en) 1997-08-20 2004-09-21 The University Of Miami Rapid tissue processor
US6960439B2 (en) 1999-06-28 2005-11-01 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6602670B2 (en) 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
CA2436910C (en) * 2000-12-01 2014-06-17 Response Genetics, Inc. Method of determining epidermal growth factor receptor and her2-neu gene expression and correlation of levels thereof with survival rates
US20020142328A1 (en) * 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
US6518416B1 (en) 2000-12-01 2003-02-11 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
US6686155B2 (en) * 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
US20040091857A1 (en) * 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
JP4303592B2 (ja) * 2001-10-12 2009-07-29 ベクトン・ディキンソン・アンド・カンパニー 生体試料を輸送する方法及びバスケット装置
WO2003031064A1 (en) 2001-10-12 2003-04-17 Becton, Dickinson And Company Method and apparatus for transporting biological samples
CN1612936A (zh) 2001-11-09 2005-05-04 苏尔斯精细医药公司 利用基因表达分布图识别、监控和治疗疾病以及鉴定生物学状态
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
CA2992643C (en) * 2002-03-13 2019-06-18 Genomic Health, Inc. Gene expression profiling in biopsied tumor tissues
JP2005534686A (ja) 2002-07-31 2005-11-17 ユニバーシティ オブ サザン カリフォルニア 疾患および処置結果の予見のための多型性
CN100374576C (zh) * 2002-10-11 2008-03-12 阿克丘勒斯生物科学股份有限公司 来自ffpe样品的基因表达分布图
US7364846B2 (en) 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
EP2363500A1 (en) 2002-11-14 2011-09-07 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative RT-PCR
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
AU2003295598B2 (en) 2002-11-15 2009-12-24 Genomic Health, Inc. Gene expression profiling of EGFR positive cancer
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US20040231909A1 (en) 2003-01-15 2004-11-25 Tai-Yang Luh Motorized vehicle having forward and backward differential structure
CA2516553C (en) * 2003-02-20 2013-04-16 Genomic Health, Inc. Use of intronic rna to measure gene expression
CA2518569C (en) 2003-03-10 2011-11-15 Expression Pathology, Inc. Liquid tissue preparation from histopathologically processed biological samples, tissues and cells
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
EP1636380A2 (en) * 2003-05-30 2006-03-22 Genomic Health, Inc. Gene expression markers for response to egfr inhibitor drugs
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
ES2609234T3 (es) 2003-06-24 2017-04-19 Genomic Health, Inc. Predicción de la probabilidad de recidiva de cáncer
US7526387B2 (en) 2003-07-10 2009-04-28 Genomic Health, Inc. Expression profile algorithm and test for cancer prognosis
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
ATE554166T1 (de) * 2003-07-25 2012-05-15 Life Technologies Corp Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
EP2216415B2 (en) * 2003-08-01 2017-01-04 Life Technologies Corporation Methods for preparing short RNA molecules
CA2542656A1 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. Qrt-pcr assay system for gene expression profiling
DE602004023104D1 (de) * 2003-10-24 2009-10-22 Univ Miami Vereinfachte gewebeverarbeitung
US20050214316A1 (en) 2003-11-13 2005-09-29 Brown Thomas P Methods of characterizing infectious bursal disease virus
CA2551267C (en) * 2003-12-23 2012-05-01 Genomic Health, Inc. Universal amplification of fragmented rna
CA2563074C (en) 2004-04-09 2014-05-20 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
US7794932B2 (en) 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
JP2007532918A (ja) * 2004-04-16 2007-11-15 チュー，ウェイシン 組織標本からの生体分子抽出装置
US20050239055A1 (en) * 2004-04-22 2005-10-27 Mariani Brian D Enterovirus primers and probes
US7332597B2 (en) * 2004-06-28 2008-02-19 University Of Kentucky Research Foundation Primers and probe to identify mycobacterium tuberculosis complex
US20060115827A1 (en) * 2004-07-01 2006-06-01 University Of Southern California Genetic markers for predicting disease and treatment outcome
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
DE102004047772B4 (de) * 2004-09-30 2018-12-13 Infineon Technologies Ag Lateraler Halbleitertransistor
EP2458015B1 (en) * 2004-09-30 2015-12-23 Epigenomics AG Method for providing DNA fragments derived from an archived sample
EP1815014B1 (en) * 2004-11-05 2012-03-21 Genomic Health, Inc. Molecular indicators of breast cancer prognosis and prediction of treatment response
JP5020088B2 (ja) * 2004-11-05 2012-09-05 ジェノミック ヘルス， インコーポレイテッド 遺伝子発現マーカーを使用する、化学療法に対する反応の予測
US20060141481A1 (en) * 2004-12-27 2006-06-29 Mariani Brian D HSV-1 and HSV-2 primers and probes
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US7449297B2 (en) * 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
WO2006119464A1 (en) * 2005-05-04 2006-11-09 University Of South Florida Predicting treatment response in cancer subjects
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1777291A1 (en) * 2005-10-20 2007-04-25 Fundacion para la Investigacion Clinica y Molecular del Cancer de Pulmon Method for the isolation of mRNA from formalin fixed, paraffin-embedded tissue
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
DE102005060738A1 (de) * 2005-12-16 2007-06-21 Qiagen Gmbh Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben
CN100366632C (zh) * 2005-12-28 2008-02-06 南方医科大学 一种石蜡包埋组织提取蛋白质的方法
JP2010508512A (ja) * 2006-10-27 2010-03-18 ジョージ メイソン インテレクチュアル プロパティーズ，インコーポレイテッド オブ フェアファックス，バージニア 転移性結腸直腸癌のアッセイ
EP2147119B1 (en) * 2007-05-03 2011-05-25 Roche Diagnostics GmbH SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
WO2008157316A1 (en) * 2007-06-14 2008-12-24 Creighton University Improved method for rna isolation
KR20100044780A (ko) * 2007-06-15 2010-04-30 유니버시티 오브 사우스 플로리다 암의 진단 및 치료 방법
CN101784663A (zh) 2007-06-22 2010-07-21 应答遗传公司 从固定的样品分离长片段rna的方法
MX2010005080A (es) 2007-11-07 2010-07-28 Genentech Inc Metodos y composiciones para determinar la sensibilidad de linfoma de celula b al tratamiento con anticuerpos anti-cd40.
EP2262893B1 (en) * 2008-04-15 2013-04-24 Universität Bern Method for optimized isolation of rna from fixed tissue
US20090298172A1 (en) * 2008-05-28 2009-12-03 Steven Paul Wheeler Histological specimen treatment apparatus and method
DE102008029356A1 (de) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
WO2010012464A1 (en) * 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
CA2745431A1 (en) * 2008-12-03 2010-06-10 Timothy J. O'leary Pressure-assisted molecular recovery (pamr) of biomolecules, pressure-assisted antigen retrieval (paar), and pressure-assisted tissue histology (path)
EP2193831A1 (de) * 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
US8846878B1 (en) 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
EP2210936A1 (en) * 2009-01-27 2010-07-28 Curetis AG Processing and analysis of viscous liquid biological samples
KR101787768B1 (ko) * 2009-04-18 2017-10-18 제넨테크, 인크. 항-ｃｄ４０ 항체를 사용한 치료에 대한 ｂ-세포 림프종의 반응성 평가를 위한 방법
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
DE202009018981U1 (de) * 2009-07-01 2015-03-11 Axagarius Gmbh & Co. Kg Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit dafür
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
US8329360B2 (en) * 2009-12-04 2012-12-11 Taiwan Semiconductor Manufacturing Company, Ltd. Method and apparatus of providing overlay
AU2010333801B2 (en) 2009-12-23 2015-12-17 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
BR112012016451A2 (pt) * 2010-01-04 2020-09-08 Giagen Gaithersburg, INC. métodos, composições, e kits para recuperação de ácidos nucleicos ou proteínas de amostras de tecido fixadas
WO2011104027A1 (en) * 2010-02-26 2011-09-01 Qiagen Gmbh Process for parallel isolation and/or purification of rna and dna
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CN102822340A (zh) 2010-04-08 2012-12-12 恰根有限公司 分离和纯化核酸的色谱设备和方法
CN102812118B (zh) 2010-04-08 2016-01-20 恰根有限公司 分离和纯化核酸的方法
US20130029336A1 (en) 2010-04-12 2013-01-31 Response Genetics, Inc. KRAS Primers and Probes
EP2388324A1 (de) * 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Kontrollmaterial für Nukleinsäureteste
EP2395082A1 (en) * 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
CN103261873A (zh) * 2010-10-12 2013-08-21 生命科技股份有限公司 制备用于ffpe的质量对照材料的方法
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
CN102146112B (zh) * 2011-01-25 2013-07-10 天根生化科技（北京）有限公司 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法
CN107255584B (zh) * 2011-02-28 2021-04-13 安捷伦科技有限公司 用于预处理包埋的生物样本的不混溶两相系统
CN102181431B (zh) * 2011-03-11 2012-12-26 大连医科大学 从石蜡包埋组织中提取总rna的方法
JP6290626B2 (ja) 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
ES2656958T3 (es) 2011-03-28 2018-03-01 Geron Corporation Medición de la longitud de telómeros en muestras fijadas en formalina, embebidas en parafina (FFPE) por PCR cuantitativa
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
HUE046506T2 (hu) 2011-06-16 2020-03-30 Univ California Szintetikus génklaszterek
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
FR2982278A1 (fr) 2011-11-03 2013-05-10 Assist Publ Hopitaux De Paris Procede de classification d'echantillons de tissus fixes et inclus en paraffine
US9416357B2 (en) 2011-11-16 2016-08-16 University Of Georgia Research Foundation, Inc. Method for isolating total RNA from cells
EP2780453B1 (en) 2011-11-17 2017-03-29 Rheonix, Inc. Microfluidic apparatus, method, and applications
CN103571824B (zh) 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
US10233440B2 (en) 2012-09-28 2019-03-19 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
CA2887575A1 (en) 2012-10-15 2014-04-24 Pioneer Hi-Bred International, Inc. Methods and compositions to enhance activity of cry endotoxins
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
US9200327B2 (en) 2012-11-30 2015-12-01 Geron Corporation Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors
BR112015023285B1 (pt) 2013-03-14 2022-03-03 Pioneer Hi-Bred International, Inc. Cassete de expressão, célula hospedeira bacteriana e método para controlar uma praga de planta do tipo coleóptero
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
KR101748867B1 (ko) * 2013-04-19 2017-06-20 주식회사 젠큐릭스 조기 유방암 예후 예측 진단용 자동화 시스템
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
MX359026B (es) 2013-08-16 2018-09-12 Pioneer Hi Bred Int Proteinas insecticidas y metodos de uso.
CA3223359A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3290904A1 (en) 2014-02-07 2026-03-02 E. I. Du Pont De Nemours And Company Insecticidal proteins and methods for their use
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
CA2961733A1 (en) 2014-09-17 2016-03-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CA2963558C (en) 2014-10-16 2023-04-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
DE102014118532B4 (de) 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
WO2016109799A2 (en) * 2014-12-31 2016-07-07 The Rockefeller University Method of rna isolation from clinical samples
CN107635396B (zh) 2015-01-15 2021-12-24 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN107529763B (zh) 2015-03-11 2021-08-20 先锋国际良种公司 Pip-72的杀昆虫组合及使用方法
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10365189B2 (en) 2015-05-07 2019-07-30 Steven Wheeler Histological specimen treatment
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
EA033170B1 (ru) * 2015-05-21 2019-09-30 Государственное Учреждение Образования "Белорусская Медицинская Академия Последипломного Образования" (Гуо "Белмапо") Способ определения экспрессии гена gstm1 в образцах из парафин-фиксированных срезов опухолевой ткани молочной железы
AU2016278142A1 (en) 2015-06-16 2017-11-30 E. I. Du Pont De Nemours And Company Compositions and methods to control insect pests
CN108602729B (zh) 2015-07-13 2022-07-05 皮沃特生物公司 改良植物性状的方法及组合物
AU2016298048B2 (en) 2015-07-24 2022-08-04 Cepheid Compositions and methods for DNA and RNA extraction from tissue samples
CA3268091A1 (en) 2015-08-06 2026-03-02 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
CN108513584A (zh) 2015-08-28 2018-09-07 先锋国际良种公司 苍白杆菌介导的植物转化
WO2017062412A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
KR101749419B1 (ko) 2015-10-15 2017-07-03 주식회사 어큐진 파라핀포매된 샘플로부터 dna를 추출하는 방법
KR102771841B1 (ko) 2015-11-06 2025-02-24 벤타나 메디컬 시스템즈, 인코포레이티드 대표 진단법
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN108699117B (zh) 2016-04-14 2023-06-23 先锋国际良种公司 具有改善的活性谱的杀昆虫多肽及其用途
CA3021391A1 (en) 2016-04-19 2017-10-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof
MX387077B (es) 2016-05-04 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para sus usos.
WO2017218207A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
ES2924552T3 (es) 2016-06-24 2022-10-07 Pioneer Hi Bred Int Elementos reguladores de las plantas y métodos de uso de los mismos
CA3026113A1 (en) 2016-07-01 2018-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
EP3555118B1 (en) 2016-12-14 2021-08-18 Pioneer Hi-Bred International Inc. Insecticidal proteins and methods for their use
WO2018119295A1 (en) 2016-12-22 2018-06-28 Ventana Medical Systems, Inc. Fully automated nucleic acid extraction method for tissue samples
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
JP7234116B2 (ja) 2017-01-12 2023-03-07 ピボット バイオ， インコーポレイテッド 植物形質を改善するための方法および組成物
WO2018140214A1 (en) 2017-01-24 2018-08-02 Pioneer Hi-Bred International, Inc. Nematicidal protein from pseudomonas
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
CA3064884A1 (en) 2017-05-26 2018-11-29 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having improved activity spectrum and uses thereof
CN107271239A (zh) * 2017-07-06 2017-10-20 浙江大学 一种溶解石蜡组织切片的方法及应用
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize
AU2018354221B2 (en) 2017-10-25 2025-04-17 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
JP2021500907A (ja) 2017-10-25 2021-01-14 ピボット バイオ， インコーポレイテッド 植物の形質の改良を目的とした窒素固定のための遺伝子標的
WO2019165245A1 (en) 2018-02-22 2019-08-29 Zymergen Inc. Method for creating a genomic library enriched for bacillus and identification of novel cry toxins
WO2019169227A1 (en) 2018-03-02 2019-09-06 Zymergen Inc. Insecticidal protein discovery platform and insecticidal proteins discovered therefrom
CA3087861A1 (en) 2018-03-02 2019-09-06 Pioneer Hi-Bred International, Inc. Plant health assay
AU2019234566B2 (en) 2018-03-14 2024-09-26 Hexima Limited Insecticidal proteins from plants and methods for their use
CN111867377B (zh) 2018-03-14 2023-05-23 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
US11702668B2 (en) 2018-05-22 2023-07-18 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
CN112739668A (zh) 2018-06-27 2021-04-30 皮沃特生物股份有限公司 包括重构固氮微生物的农业组合物
KR20210060434A (ko) 2018-07-11 2021-05-26 피벗 바이오, 인크. 리모델링된 미생물에 의한 시간적 및 공간적 표적화 동적 질소 전달
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US11878999B2 (en) 2018-08-29 2024-01-23 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020190363A1 (en) 2019-03-19 2020-09-24 Massachusetts Institute Of Technology Control of nitrogen fixation in rhizobia that associate with cereals
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN110376036A (zh) * 2019-07-30 2019-10-25 河南赛诺特生物技术有限公司 一种肿瘤细胞系免疫组化对照品的制备方法及肿瘤细胞系免疫组化对照品
CN110361242B (zh) * 2019-08-14 2022-01-18 武汉赛维尔生物科技有限公司 一种用于眼球组织的固定液以及眼球组织制片的预处理方法
WO2021076346A1 (en) 2019-10-18 2021-04-22 Pioneer Hi-Bred International, Inc. Maize event dp-202216-6 and dp-023211-2 stack
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021133849A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
TWI906251B (zh) 2020-02-04 2025-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關之方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115335519A (zh) 2020-03-31 2022-11-11 凯杰有限公司 从固定的生物样品纯化核酸
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
WO2021212042A1 (en) 2020-04-16 2021-10-21 10X Genomics, Inc. Compositions and methods for use with fixed samples
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
AU2020445067A1 (en) 2020-05-01 2022-12-01 Pivot Bio, Inc. Measurement of nitrogen fixation and incorporation
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4553168A3 (en) 2020-05-22 2025-08-27 10x Genomics, Inc. Spatial analysis to detect sequence variants
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12168774B2 (en) 2020-07-14 2024-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20230287475A1 (en) 2020-07-31 2023-09-14 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
BR112023002603A2 (pt) 2020-08-10 2023-04-04 Pioneer Hi Bred Int Elementos reguladores de plantas e métodos de uso dos mesmos
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4244379A1 (en) 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CN114854824A (zh) * 2021-02-03 2022-08-05 深圳华大基因股份有限公司 建库方法及应用
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023028064A2 (en) * 2021-08-25 2023-03-02 Matchstick Technologies, Inc. Microplate cycling thermo sonicator with programmable ultrasound, heating, and cooling for multi-analytical applications
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
TW202345696A (zh) 2022-05-18 2023-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關的方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2025178772A1 (en) 2024-02-23 2025-08-28 Genective Sa Insecticidal proteins compositions and methods of use
WO2025193453A1 (en) 2024-03-14 2025-09-18 Genective Sa Insecticidal proteins compositions and methods of use
WO2025235220A1 (en) 2024-05-08 2025-11-13 Genective Sa Insecticidal proteins compositions and methods of use
WO2025264577A1 (en) 2024-06-20 2025-12-26 Genective Sa Insecticidal proteins, compositions and methods of use
WO2025264584A1 (en) 2024-06-20 2025-12-26 Genective Sa Insecticidal proteins, compositions and methods of use
WO2026006045A1 (en) 2024-06-25 2026-01-02 Genective Sa Insecticidal proteins, compositions and methods of use
WO2026043743A1 (en) 2024-08-21 2026-02-26 Genective Sa Insecticidal proteins compositions and methods of use
WO2026055006A2 (en) 2024-09-03 2026-03-12 Genective Sa Insecticidal proteins compositions and methods of use
WO2026076007A1 (en) 2024-10-04 2026-04-09 Genective Sa Insecticidal proteins compositions and methods of use

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830969A (en) 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5620852A (en) 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
US5284940A (en) 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5502166A (en) 1992-02-26 1996-03-26 Mitsubishi Chemical Corporation NMDH receptor proteins and genes encoding the same
WO1994018156A1 (en) 1993-02-01 1994-08-18 University Of Iowa Research Foundation Quartenary amine surfactants and methods of using same in isolation of rna
US5876978A (en) * 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5643767A (en) 1994-05-02 1997-07-01 The Rockefeller University Process for isolating cellular components
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
CA2153215A1 (en) 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
US5707802A (en) * 1995-01-13 1998-01-13 Ciba Corning Diagnostics Corp. Nucleic acid probes for the detection and identification of fungi
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5998151A (en) 1995-12-01 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Methods for predicting the efficacy of a chemotherapeutic regimen for gastrointestinal cancers using antibodies specific for thymidylate synthase
US20020090642A1 (en) * 1995-12-11 2002-07-11 Patrick G. Johnston Methods for determining the prognosis of breast cancer using antibodies specific for thymidylate synthase
DE29601618U1 (de) 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
EP0791654A1 (en) 1996-02-21 1997-08-27 Jürgen A. Dr. Richt Polypeptides corresponding to the amino acid sequences of proteins p57 or p9.5 of Borna disease virus, nucleic acid fragments coding therefore and their use for diagnostic and immunization purposes
WO1997043407A1 (en) 1996-05-10 1997-11-20 Phylomed Corporation Methods for oxidizing disulfide bonds using ozone
JP2001522238A (ja) * 1997-03-31 2001-11-13 アボツト・ラボラトリーズ 胃腸管の疾患の検出に有用な試薬および方法
ATE314488T1 (de) * 1999-03-18 2006-01-15 Exiqon As Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

Also Published As

Publication number Publication date
US6610488B2 (en) 2003-08-26
US20010029018A1 (en) 2001-10-11
AU1578501A (en) 2001-07-03
EP1743939A2 (en) 2007-01-17
US20020009795A1 (en) 2002-01-24
US7919280B2 (en) 2011-04-05
EP2363477A2 (en) 2011-09-07
ES2373149T3 (es) 2012-01-31
CA2394618A1 (en) 2001-06-28
CN1420927A (zh) 2003-05-28
US20020009794A1 (en) 2002-01-24
EP2363477A3 (en) 2013-07-31
DE60032634T2 (de) 2007-10-04
EA200200698A1 (ru) 2003-12-25
US6428963B2 (en) 2002-08-06
US7998707B2 (en) 2011-08-16
DK1743939T3 (da) 2011-12-12
DE60032634D1 (de) 2007-02-08
EA006827B1 (ru) 2006-04-28
KR100723328B1 (ko) 2007-05-31
NO20022945L (no) 2002-08-06
IL150116A (en) 2012-01-31
KR20030027878A (ko) 2003-04-07
EP1743939A3 (en) 2007-05-09
ATE349518T1 (de) 2007-01-15
EP1242594A1 (en) 2002-09-25
EP1743939B1 (en) 2011-09-07
IL150116A0 (en) 2002-12-01
TWI277652B (en) 2007-04-01
US7960147B2 (en) 2011-06-14
EP1242594B1 (en) 2006-12-27
CN1891836B (zh) 2010-04-21
NO20022945D0 (no) 2002-06-19
ATE523590T1 (de) 2011-09-15
EA008832B1 (ru) 2007-08-31
AU2006202986B2 (en) 2008-12-04
US6248535B1 (en) 2001-06-19
HK1055446A1 (en) 2004-01-09
US20040091910A1 (en) 2004-05-13
AU784565B2 (en) 2006-05-04
AU784565C (en) 2007-02-01
US6613518B2 (en) 2003-09-02
AU2006202986A1 (en) 2006-08-10
CN1269959C (zh) 2006-08-16
HK1098178A1 (zh) 2007-07-13
EA200501772A1 (ru) 2006-10-27
NO330802B1 (no) 2011-07-18
MXPA02006145A (es) 2004-08-23
WO2001046402A1 (en) 2001-06-28
US20090035761A1 (en) 2009-02-05
AR027036A1 (es) 2003-03-12
US20060199197A1 (en) 2006-09-07
US20040018551A1 (en) 2004-01-29
CN1891836A (zh) 2007-01-10
JP2003525033A (ja) 2003-08-26
CA2394618C (en) 2013-05-07

Similar Documents

Publication Publication Date Title
BR0016569A (pt) Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina
Black et al. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing
DE69632466D1 (de) Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen
DE69929542D1 (de) Komplexitätsmanagement und analyse genomischer dna
ATE269416T1 (de) Verwendung von vereinigten sonden zur genetischen analyse
ATE503843T1 (de) Gesamt-chromosom analyse von protein-dns wechselwirkungen
NO20011505L (no) Alzheimer's sykdom-sekretase
DK0979308T3 (da) Fremgangsåde til fremstilling af et diagnostisk standard gen-transkriptmönster
EP0831879A4 (en) METHOD FOR IDENTIFYING AND ISOLATING A MOLECULE AND MOLECULE DETECTED BY IT
ATE212661T1 (de) Durch enzymkatalyse erhaltene therapeutische substanzen.
SE9601318D0 (sv) Method for nucleic acid analysis
DK1005540T3 (da) IKK-beta-proteiner, nukleinsyrer og fremgangsmåder
ATE214104T1 (de) Verfahren zur charakterisierung von nukleinsäuremolekulen, das die erzeugung von verlängerbaren aufwärtsgelegenen dns-fragmenten, die durch spaltung der nukleinsäure an einer abasischen stelle entsteht, umfasst
WO2004044164A3 (en) Method for identifying risk of melanoma and treatments thereof
SE9403805D0 (sv) Method of preparing oligonucleotide probes or primers, vector therefor and use thereof
Lannutti et al. Probing the Protein− DNA Contacts of a Yeast RNA Polymerase III Transcription Complex in a Crude Extract: Solid Phase Synthesis of DNA Photoaffinity Probes Containing a Novel Photoreactive Deoxycytidine Analog
Mekhovich et al. Rate of Incision of N-Acetyl-2-aminofluorene and N-2-Aminofluorene Adducts by UvrABC Nuclease Is Adduct-and Sequence-Specific: Comparison of the Rates of UvrABC Nuclease Incision and Protein− DNA Complex Formation
NO994705L (no) Preparering av blodpröver for deteksjon av homocystein og/eller folat
EP0382261A3 (en) Dna probes to vntr loci
Dème et al. Unexpected metal ion requirements specific for catalysis of the branching reaction in a group II intron
CA2275853A1 (en) Computer directed identification of paraproteins
DE60025011D1 (de) Dna polymerase lambda und ihre verwendungen
ATE272718T1 (de) Verfahren zur bestimmung von disaccharidasen
Giovine et al. Synthesis and characterization of a specific peptide nucleic acid that inhibits expression of inducible NO synthase
BR0115728A (pt) Análise da expressão de ácidos nucléicos kiaa e polipeptìdeos úteis no diagnóstico e tratamento de cáncer de próstata

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]