BR0303224A - Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma - Google Patents

Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma

Info

Publication number
BR0303224A
BR0303224A BR0303224-8A BR0303224A BR0303224A BR 0303224 A BR0303224 A BR 0303224A BR 0303224 A BR0303224 A BR 0303224A BR 0303224 A BR0303224 A BR 0303224A
Authority
BR
Brazil
Prior art keywords
methods
energy transfer
resonance energy
fluorescent resonance
nucleotide
Prior art date
Application number
BR0303224-8A
Other languages
English (en)
Inventor
Gerardo Turcatti
Original Assignee
Applied Research Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of BR0303224A publication Critical patent/BR0303224A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"DETECçãO DE NUCLEOTìDEOS POR TRANSFERêNCIA DE ENERGIA DE RESSONâNCIA FLUORESCENTE E MéTODOS PARA A MESMA". A presente invenção fornece novos processos para a detecção da incorporação de um nucleotídeo após uma reação de extensão de bases isoladas. Os processos fazem uso do efeito da Transferência de Energia de Ressonância Fluorescente (FRET) gerado por dois fluoróforos ligados ao mesmo filamento de ácido nucléico. Os processos podem ser utilizados, em solução ou em um suporte sólido, para aplicações que requerem a identificação rápida e precisa de um nucleotídeo em uma posição específica de um ácido nucléico, como para estudos de genotipagem.
BR0303224-8A 2002-02-11 2003-02-07 Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma BR0303224A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02100124 2002-02-11
PCT/EP2003/050013 WO2003068986A1 (en) 2002-02-11 2003-02-07 Nucleotide detection by fluorescence resonance energy transfer and methods thereof

Publications (1)

Publication Number Publication Date
BR0303224A true BR0303224A (pt) 2004-07-06

Family

ID=27675737

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0303224-8A BR0303224A (pt) 2002-02-11 2003-02-07 Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma

Country Status (4)

Country Link
AU (1) AU2003226829A1 (pt)
BR (1) BR0303224A (pt)
NO (1) NO20034592L (pt)
WO (1) WO2003068986A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP2041535A4 (en) * 2006-06-29 2010-08-04 Cdex Inc METHOD AND APPARATUS FOR MOLECULAR SPECTRUM RECOGNITION, TESTING AND CLASSIFICATION USING ULTRAVIOLETT TO FAST INFRARED AMPLIFIED LIGHT EMISSION SPECTROSCOPY

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
AU710425B2 (en) * 1995-12-18 1999-09-23 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
EP1715063B1 (en) * 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination

Also Published As

Publication number Publication date
NO20034592L (no) 2003-12-09
WO2003068986A1 (en) 2003-08-21
AU2003226829A1 (en) 2003-09-04
NO20034592D0 (no) 2003-10-13

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]