BRPI0212140B8 - "substrato de toxina de botulinum de sorotipo a (bont/a), bem como método de determinar atividade de protease de toxina clostridial". - Google Patents

"substrato de toxina de botulinum de sorotipo a (bont/a), bem como método de determinar atividade de protease de toxina clostridial".

Info

Publication number
BRPI0212140B8
BRPI0212140B8 BRPI0212140A BRPI0212140A BRPI0212140B8 BR PI0212140 B8 BRPI0212140 B8 BR PI0212140B8 BR PI0212140 A BRPI0212140 A BR PI0212140A BR PI0212140 A BRPI0212140 A BR PI0212140A BR PI0212140 B8 BRPI0212140 B8 BR PI0212140B8
Authority
BR
Brazil
Prior art keywords
clostridial toxin
well
protease activity
donor fluorophore
bont
Prior art date
Application number
BRPI0212140A
Other languages
English (en)
Inventor
Fernandez-Salas Ester
Roger Aoki Kei
E Steward Lance
Original Assignee
Allergan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27613894&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0212140(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Allergan Inc filed Critical Allergan Inc
Publication of BR0212140A publication Critical patent/BR0212140A/pt
Publication of BRPI0212140B1 publication Critical patent/BRPI0212140B1/pt
Publication of BRPI0212140B8 publication Critical patent/BRPI0212140B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

"ensaios de protease por fret para toxinas clostridiais". a presente invenção fornece substratos de toxina clostridial úteis no ensaio para a atividade de protease de qualquer toxina clostridial, incluindo toxinas de botulinum de todos os sorotipos como também toxinas de tétano. um substrato de toxina clostridial da invenção contém um fluoróforo doador; um aceptor tendo um espectro de absorbância que sobrepõe o espectro de emissão do fluoróforo doador; e uma seqüência de reconhecimento de toxina clostridial que inclui um sítio de clivagem, onde o sítio de clivagem intervém entre o fluoróforo doador e o aceptor e onde, sob as condições apropriadas, a transferência de energia de ressonância é exibida entre o fluoróforo doador e o aceptor.
BRPI0212140A 2001-08-28 2002-08-22 "substrato de toxina de botulinum de sorotipo a (bont/a), bem como método de determinar atividade de protease de toxina clostridial". BRPI0212140B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/942,098 US7332567B2 (en) 2001-08-28 2001-08-28 Fret protease assays for clostridial toxins
PCT/US2002/027212 WO2004031773A1 (en) 2001-08-28 2002-08-22 Fret protease assays for clostridial toxins

Publications (3)

Publication Number Publication Date
BR0212140A BR0212140A (pt) 2007-01-09
BRPI0212140B1 BRPI0212140B1 (pt) 2014-11-11
BRPI0212140B8 true BRPI0212140B8 (pt) 2021-07-27

Family

ID=27613894

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0212140A BRPI0212140B8 (pt) 2001-08-28 2002-08-22 "substrato de toxina de botulinum de sorotipo a (bont/a), bem como método de determinar atividade de protease de toxina clostridial".

Country Status (9)

Country Link
US (10) US7332567B2 (pt)
EP (2) EP1438586B1 (pt)
JP (3) JP2005520000A (pt)
AT (2) ATE541213T1 (pt)
AU (1) AU2002368109B8 (pt)
BR (1) BRPI0212140B8 (pt)
CA (1) CA2462686C (pt)
DK (2) DK1438586T3 (pt)
WO (1) WO2004031773A1 (pt)

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Also Published As

Publication number Publication date
AU2002368109A1 (en) 2004-04-23
BRPI0212140B1 (pt) 2014-11-11
US8053208B2 (en) 2011-11-08
JP2005520000A (ja) 2005-07-07
DK1901069T3 (da) 2012-03-12
BR0212140A (pt) 2007-01-09
US20100081157A1 (en) 2010-04-01
US8048643B2 (en) 2011-11-01
US20090042231A1 (en) 2009-02-12
DK1438586T3 (da) 2012-03-19
EP1438586A4 (en) 2005-03-16
CA2462686A1 (en) 2004-04-15
US20100075358A1 (en) 2010-03-25
EP1438586A1 (en) 2004-07-21
US7332567B2 (en) 2008-02-19
US8013113B2 (en) 2011-09-06
ATE541213T1 (de) 2012-01-15
ATE540317T1 (de) 2012-01-15
JP2008201786A (ja) 2008-09-04
US20080032318A1 (en) 2008-02-07
US20100075357A1 (en) 2010-03-25
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US20100081158A1 (en) 2010-04-01
WO2004031773A1 (en) 2004-04-15
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US8053209B2 (en) 2011-11-08
EP1901069A1 (en) 2008-03-19
JP2008174567A (ja) 2008-07-31
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US20030143651A1 (en) 2003-07-31
US8003753B2 (en) 2011-08-23
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US20100075346A1 (en) 2010-03-25

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