BRPI0411675A - método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas - Google Patents

método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas

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Publication number
BRPI0411675A
BRPI0411675A BRPI0411675-5A BRPI0411675A BRPI0411675A BR PI0411675 A BRPI0411675 A BR PI0411675A BR PI0411675 A BRPI0411675 A BR PI0411675A BR PI0411675 A BRPI0411675 A BR PI0411675A
Authority
BR
Brazil
Prior art keywords
expression
sym
producing
nucleic acid
interest
Prior art date
Application number
BRPI0411675-5A
Other languages
English (en)
Inventor
Stephen K Picataggio
Quinn Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BRPI0411675A publication Critical patent/BRPI0411675A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODO PARA A EXPRESSãO DE UMA REGIãO DE CODIFICAçãO DE INTERESSE, MéTODO PARA A PRODUçãO DE UM áCIDO GRAXO <sym>-3 OU <sym>-6, E MOLéCULAS DE áCIDO NUCLéICO ISOLADAS". Foi verificado que as regiões de promotor associadas com os genes de gliceraldeído-3-fosfato deidrpgenase (gpd) de Yarrowia lipolytica e de fosfoglicerato mutase (gpm) são particularmente eficazes para a expressão de genes heterólogos em levedura oleaginosa. Foi demonstrado que as regiões de promotor da invenção dirigem a expressão de alto nível dos genes envolvidos na produção de ácidos graxos <sym>3- e <sym>-6.
BRPI0411675-5A 2003-06-25 2004-06-23 método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas BRPI0411675A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48226303P 2003-06-25 2003-06-25
PCT/US2004/020432 WO2005003310A2 (en) 2003-06-25 2004-06-23 Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast

Publications (1)

Publication Number Publication Date
BRPI0411675A true BRPI0411675A (pt) 2006-08-29

Family

ID=33563842

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0411675-5A BRPI0411675A (pt) 2003-06-25 2004-06-23 método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas

Country Status (9)

Country Link
US (2) US7259255B2 (pt)
EP (1) EP1636368A4 (pt)
JP (1) JP2007525969A (pt)
CN (1) CN1842598A (pt)
AU (1) AU2004254361A1 (pt)
BR (1) BRPI0411675A (pt)
CA (1) CA2526896A1 (pt)
NO (1) NO20060422L (pt)
WO (1) WO2005003310A2 (pt)

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Also Published As

Publication number Publication date
WO2005003310A2 (en) 2005-01-13
CN1842598A (zh) 2006-10-04
US20050014270A1 (en) 2005-01-20
EP1636368A4 (en) 2007-10-10
NO20060422L (no) 2006-03-21
AU2004254361A1 (en) 2005-01-13
EP1636368A2 (en) 2006-03-22
US7259255B2 (en) 2007-08-21
US20080220474A1 (en) 2008-09-11
CA2526896A1 (en) 2005-01-13
JP2007525969A (ja) 2007-09-13
WO2005003310A3 (en) 2005-03-17

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