BRPI0411675A - método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas - Google Patents

método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas

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Publication number
BRPI0411675A
BRPI0411675A BRPI0411675-5A BRPI0411675A BRPI0411675A BR PI0411675 A BRPI0411675 A BR PI0411675A BR PI0411675 A BRPI0411675 A BR PI0411675A BR PI0411675 A BRPI0411675 A BR PI0411675A
Authority
BR
Brazil
Prior art keywords
expression
sym
producing
nucleic acid
interest
Prior art date
Application number
BRPI0411675-5A
Other languages
English (en)
Inventor
Stephen K Picataggio
Quinn Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BRPI0411675A publication Critical patent/BRPI0411675A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODO PARA A EXPRESSãO DE UMA REGIãO DE CODIFICAçãO DE INTERESSE, MéTODO PARA A PRODUçãO DE UM áCIDO GRAXO <sym>-3 OU <sym>-6, E MOLéCULAS DE áCIDO NUCLéICO ISOLADAS". Foi verificado que as regiões de promotor associadas com os genes de gliceraldeído-3-fosfato deidrpgenase (gpd) de Yarrowia lipolytica e de fosfoglicerato mutase (gpm) são particularmente eficazes para a expressão de genes heterólogos em levedura oleaginosa. Foi demonstrado que as regiões de promotor da invenção dirigem a expressão de alto nível dos genes envolvidos na produção de ácidos graxos <sym>3- e <sym>-6.
BRPI0411675-5A 2003-06-25 2004-06-23 método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas BRPI0411675A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48226303P 2003-06-25 2003-06-25
PCT/US2004/020432 WO2005003310A2 (en) 2003-06-25 2004-06-23 Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast

Publications (1)

Publication Number Publication Date
BRPI0411675A true BRPI0411675A (pt) 2006-08-29

Family

ID=33563842

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0411675-5A BRPI0411675A (pt) 2003-06-25 2004-06-23 método para a expressão de uma região de codificação de interesse, método para a produção de um ácido graxo w-3 ou w-6, e moléculas de ácido nucléico isoladas

Country Status (9)

Country Link
US (2) US7259255B2 (pt)
EP (1) EP1636368A4 (pt)
JP (1) JP2007525969A (pt)
CN (1) CN1842598A (pt)
AU (1) AU2004254361A1 (pt)
BR (1) BRPI0411675A (pt)
CA (1) CA2526896A1 (pt)
NO (1) NO20060422L (pt)
WO (1) WO2005003310A2 (pt)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2542574C (en) 2003-11-12 2014-03-18 E. I. Du Pont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast
WO2005049805A2 (en) * 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
WO2005118814A2 (en) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
CA2624661C (en) 2005-11-23 2015-06-30 E.I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
JP5967861B2 (ja) * 2007-04-03 2016-08-10 オキシレイン ユーケー リミテッド 分子のグリコシル化
US7790156B2 (en) 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
EP3546588A3 (en) 2007-06-01 2019-12-18 Corbion Biotech, Inc. Recombinant microalgal cell and method of producing lipids using said cell
DE102007031947A1 (de) * 2007-07-06 2009-01-08 Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz Allergene aus Aspergillus versicolor und Verfahren zum Nachweis einer innenraumbedingten Schimmelpilzallergie
AU2009228322B2 (en) * 2008-03-27 2013-09-26 Alliance For Sustainable Energy, Llc High expression Zymomonas promoters
CA2729626C (fr) * 2008-07-11 2017-12-12 Jean-Marc Nicaud Nouvelles souches de levure mutantes capables d'accumuler une grande quantite de lipides
EP2370555B1 (en) 2008-11-28 2016-04-20 Solazyme, Inc. Production of tailored oils in heterotrophic microorganisms
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
BRPI1009649A2 (pt) 2009-06-16 2016-03-15 Du Pont célula hospedeira yarrowia sp, recombinante, método para fabricar um óleo microbiano e célula hospedeira recombinante para produção de um óleo
DK2442666T3 (da) 2009-06-16 2015-07-13 Du Pont Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
EP3192873A1 (en) * 2009-09-29 2017-07-19 Intrexon Actobiotics NV Lactobacillus and streptococcus promoters and uses thereof
US20130053550A1 (en) 2009-11-19 2013-02-28 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
EP2327776A1 (en) 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp
SG185780A1 (en) 2010-05-28 2013-01-30 Solazyme Inc Tailored oils produced from recombinant heterotrophic microorganisms
CA2807834C (en) 2010-08-26 2019-06-25 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
JP2013535988A (ja) 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
JP5963749B2 (ja) 2010-08-26 2016-08-03 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 変異δ９エロンガーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
JP6131190B2 (ja) 2010-09-29 2017-05-17 オキシレイン ユーケー リミテッド リン酸化ｎ−グリカンの脱マンノシル化
BR112013007616B1 (pt) 2010-09-29 2023-01-24 Oxyrane Uk Limited Métodos para retirar o cap ("uncapping") de porções de manose-1- fosfo-6-manose e desmanosilar n-glicanos fosforilados em uma glicoproteína, para direcionar uma glicoproteína para o interior de uma célula de mamífero, e para converter uma glicoproteína de uma primeira forma que não se liga substancialmente a um receptor de manose-6-fosfato em uma segunda forma que se liga substancialmente a um receptor de manose-6-fosfato em uma célula de mamífero
AU2011323288B2 (en) 2010-11-03 2016-05-26 Corbion Biotech, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
AU2011353002A1 (en) 2010-12-30 2013-05-30 E. I. Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
CA2825691C (en) 2011-02-02 2020-08-25 Solazyme, Inc. Tailored oils produced from recombinant oleaginous microorganisms
US8969049B2 (en) 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
EP2694665A1 (en) 2011-04-01 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
EP2694657A1 (en) 2011-04-05 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
CN103608450A (zh) 2011-05-06 2014-02-26 索拉兹米公司 基因工程改造的代谢木糖的微生物
JP2014516537A (ja) 2011-05-26 2014-07-17 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え油性微生物における、その中のオイル含量を増加させるためのカレオシンの発現
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
CA2867237C (en) 2012-03-15 2024-01-02 Wouter Vervecken Methods and materials for treatment of pompe's disease
SG10201702442RA (en) 2012-04-18 2017-05-30 Terravia Holdings Inc Tailored oils
US9719114B2 (en) 2012-04-18 2017-08-01 Terravia Holdings, Inc. Tailored oils
US10106802B2 (en) 2013-03-14 2018-10-23 Temasek Life Sciences Laboratory Limited Polynucleotide sequences from rhodosporidium and rhodotorula and use thereof
SG11201507557UA (en) 2013-03-15 2015-10-29 Lonza Ag Constitutive promoter
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
JP6517196B2 (ja) 2013-10-04 2019-05-22 テラヴィア ホールディングス， インコーポレイテッド 調整油
BR112017000414A2 (pt) 2014-07-10 2017-11-07 Terravia Holdings Inc genes cetoacil-acp sintase e utilizações dos mesmos
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
US20180362961A1 (en) 2015-12-11 2018-12-20 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
EP3708665A1 (en) 2015-12-18 2020-09-16 Danisco US Inc. Methods and compositions for t-rna based guide rna expression
EP3784791A1 (en) 2018-04-23 2021-03-03 DuPont Nutrition Biosciences ApS Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
US11572546B2 (en) 2019-07-29 2023-02-07 Ginkgo Bioworks, Inc. Methods and compositions involving promoters derived from Yarrowia lipolytica
WO2022115503A1 (en) 2020-11-25 2022-06-02 Dupont Nutrition Biosciences Aps Treatment and prevention of coronavirus infection

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE259637C (pt)
DE285370C (pt)
DE267999C (pt)
DE285372C (pt)
DE279267C (pt)
DE227448C (pt)
DE275480C (pt)
NZ190194A (en) 1978-05-08 1982-02-23 Cpc International Inc Production of oil by fermenting yeast in fatty acids
US4937189A (en) 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
SU1454852A1 (ru) 1987-05-19 1989-01-30 Институт Микробиологии Им.Августа Кирхенштейна Штамм дрожжей JaRRoWIa LIроLYтIса-продуцент липазы и питательна среда дл его культивировани
PL160027B1 (pl) 1989-03-23 1993-01-29 Akad Rolnicza Sposób jednoczesnego otrzymywania kwasu cytrynowego i kwasu izocytrynowego PL
EP0578388B1 (en) 1992-06-25 1998-11-04 INTERNATIONAL FLAVORS &amp; FRAGRANCES INC. Fermentation process for preparing 10-hydroxy-C18-carboxylic acid and gamma-dodecalactone derivatives
IT1256043B (it) 1992-08-12 1995-11-21 Ceppo mutante di yarrowia lipolytica e procedimento per la produzione di acido citrico
FR2734843A1 (fr) 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
EP0770683A1 (en) 1995-10-04 1997-05-02 Mitsubishi Chemical Corporation Method for producing erythritol
JPH09252790A (ja) 1996-03-21 1997-09-30 Japan Energy Corp 発酵法によるピルビン酸の製造方法
EP0922109A1 (en) * 1996-05-21 1999-06-16 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
RU2090611C1 (ru) 1996-09-27 1997-09-20 Финогенова Татьяна Васильевна Штамм дрожжей yarrowia lipolytica - продуцент лимонной кислоты, способ получения лимонной кислоты и способ выделения цитрата натрия
US6432684B1 (en) * 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
EP0997533A1 (de) * 1998-10-24 2000-05-03 Haarmann & Reimer Gmbh Verfahren zur Gewinnung von Gamma-Decalacton
AU5876300A (en) * 1999-06-24 2001-01-09 Brady G. Miller Pichia methanolica glyceraldehyde 3-phosphate dehydrogenase 1 promoter and terminator
PT1276874E (pt) 2000-04-28 2008-01-11 Mayoly Spindler Lab Clonagem e expressão de uma lipase extracelular resistente aos ácidos de yarrowia lipolytica
KR20020029369A (ko) 2000-05-19 2002-04-18 후루타 타케시 형질전환체 및 이것을 이용한 폴리에스테르의 제조방법
WO2005049805A2 (en) * 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast

Also Published As

Publication number Publication date
WO2005003310A2 (en) 2005-01-13
NO20060422L (no) 2006-03-21
EP1636368A4 (en) 2007-10-10
AU2004254361A1 (en) 2005-01-13
US7259255B2 (en) 2007-08-21
WO2005003310A3 (en) 2005-03-17
US20080220474A1 (en) 2008-09-11
CA2526896A1 (en) 2005-01-13
CN1842598A (zh) 2006-10-04
US20050014270A1 (en) 2005-01-20
JP2007525969A (ja) 2007-09-13
EP1636368A2 (en) 2006-03-22

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