BRPI0412272A - composições de pares de lisil-trna e aminoacil-trna sintetase ortogonais e usos destes - Google Patents

composições de pares de lisil-trna e aminoacil-trna sintetase ortogonais e usos destes

Info

Publication number
BRPI0412272A
BRPI0412272A BRPI0412272-0A BRPI0412272A BRPI0412272A BR PI0412272 A BRPI0412272 A BR PI0412272A BR PI0412272 A BRPI0412272 A BR PI0412272A BR PI0412272 A BRPI0412272 A BR PI0412272A
Authority
BR
Brazil
Prior art keywords
trna
orthogonal
aminoacyl
lysyl
synthetase pair
Prior art date
Application number
BRPI0412272-0A
Other languages
English (en)
Inventor
Christopher J Anderson
Ning Wu
Stephen Santoro
Peter G Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of BRPI0412272A publication Critical patent/BRPI0412272A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"COMPOSIçõES DE PARES DE LISIL-TRNA E AMINOACIL-TRNA SINTETASE ORTOGONAIS E USOS DESTES". Composições e métodos de produzir componentes de maquinário bio-sintético de proteínas que incluem lisil-tRNAs, ortogonal lisil-aminoacil-tRNA sintetases ortogonais, e pares ortogonais de lisil-tRNAs/sintetases, que incorporam homoglutaminas em proteínas são providos em resposta a um códon de base quatro. Métodos para identificar esses pares ortogonais são também providos juntamente com métodos de produção de proteínas com homoglutaminas usando esses pares ortogonais.
BRPI0412272-0A 2003-07-07 2004-07-07 composições de pares de lisil-trna e aminoacil-trna sintetase ortogonais e usos destes BRPI0412272A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48545103P 2003-07-07 2003-07-07
US52881503P 2003-12-10 2003-12-10
US53714904P 2004-01-15 2004-01-15
PCT/US2004/022187 WO2005019415A2 (en) 2003-07-07 2004-07-07 Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof

Publications (1)

Publication Number Publication Date
BRPI0412272A true BRPI0412272A (pt) 2006-09-05

Family

ID=34222340

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0412272-0A BRPI0412272A (pt) 2003-07-07 2004-07-07 composições de pares de lisil-trna e aminoacil-trna sintetase ortogonais e usos destes

Country Status (14)

Country Link
US (4) US7638297B2 (pt)
EP (2) EP1649004A4 (pt)
JP (2) JP5192150B2 (pt)
CN (1) CN101076598B (pt)
AT (2) ATE471385T1 (pt)
AU (1) AU2004267359B2 (pt)
BR (1) BRPI0412272A (pt)
CA (1) CA2531146A1 (pt)
DE (2) DE602004011789T2 (pt)
ES (1) ES2301099T3 (pt)
MX (1) MXPA06000242A (pt)
PT (2) PT1666604E (pt)
SI (1) SI1666604T1 (pt)
WO (1) WO2005019415A2 (pt)

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