BRPI0412308A - métodos para a sìntese estereosseletiva e enriquecimento enantiomérico de ß-aminoácidos - Google Patents
métodos para a sìntese estereosseletiva e enriquecimento enantiomérico de ß-aminoácidosInfo
- Publication number
- BRPI0412308A BRPI0412308A BRPI0412308-5A BRPI0412308A BRPI0412308A BR PI0412308 A BRPI0412308 A BR PI0412308A BR PI0412308 A BRPI0412308 A BR PI0412308A BR PI0412308 A BRPI0412308 A BR PI0412308A
- Authority
- BR
- Brazil
- Prior art keywords
- beta
- methods
- amino acids
- phenylalanine
- new
- Prior art date
Links
- 230000015572 biosynthetic process Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 230000000707 stereoselective effect Effects 0.000 title abstract 3
- 238000003786 synthesis reaction Methods 0.000 title abstract 3
- 150000001576 beta-amino acids Chemical class 0.000 title abstract 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 2
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 abstract 2
- UJOYFRCOTPUKAK-QMMMGPOBSA-N (S)-3-ammonio-3-phenylpropanoate Chemical compound OC(=O)C[C@H](N)C1=CC=CC=C1 UJOYFRCOTPUKAK-QMMMGPOBSA-N 0.000 abstract 1
- 241000252867 Cupriavidus metallidurans Species 0.000 abstract 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 abstract 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 abstract 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
- 241001478284 Variovorax paradoxus Species 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 229960003767 alanine Drugs 0.000 abstract 1
- 229940024606 amino acid Drugs 0.000 abstract 1
- 229960002989 glutamic acid Drugs 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229960005190 phenylalanine Drugs 0.000 abstract 1
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 abstract 1
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 abstract 1
- 229960001327 pyridoxal phosphate Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
- C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"MéTODOS PARA A SìNTESE ESTEREOSSELETIVA E ENRIQUECIMENTO ENANTIOMéRICO DE <225>-AMINOáCIDOS". A presente invenção refere-se a métodos para a síntese estereoespecífica e para o enriquecimento enantiomérico de beta-aminoácidos. Uma nova D-beta-aminotransferase, que apresenta estereosseletividade para D-beta-fenilalanina, (ácido D-3-amino-3-fenilpropanóico) , foi purificada a partir de uma nova cepa isolada de Variovorax paradoxus. uma nova L-beta-aminotransferase foi purificada a partir de uma nova cepa isolada de Alcaligenes eutrophus. As D- e L-beta-aminotransferases podem ser utilizadas para facilitar a biossíntese estereosseletiva de beta-D-fenilalanina ou beta-L-fenilalanina, a partir de uma mistura de ácido L-glutâmico ou L-alanina, respectivamente, e ácido 3-ceto-3-fenilpropanóico na presença do cofator piridoxal fosfato.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US48603203P | 2003-07-10 | 2003-07-10 | |
| US49962203P | 2003-09-02 | 2003-09-02 | |
| PCT/IB2004/002183 WO2005005633A2 (en) | 2003-07-10 | 2004-06-30 | Methods for the stereoselective synthesis and enantiomeric enrichment of b-amino acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0412308A true BRPI0412308A (pt) | 2006-06-20 |
Family
ID=34068231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0412308-5A BRPI0412308A (pt) | 2003-07-10 | 2004-06-30 | métodos para a sìntese estereosseletiva e enriquecimento enantiomérico de ß-aminoácidos |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20050009151A1 (pt) |
| EP (1) | EP1646718A2 (pt) |
| JP (1) | JP2007526751A (pt) |
| BR (1) | BRPI0412308A (pt) |
| CA (1) | CA2529509A1 (pt) |
| MX (1) | MXPA06000367A (pt) |
| WO (1) | WO2005005633A2 (pt) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009165355A (ja) * | 2006-04-28 | 2009-07-30 | Ajinomoto Co Inc | Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法 |
| HUE052297T2 (hu) | 2009-01-08 | 2021-04-28 | Codexis Inc | Transzamináz polipeptidek |
| WO2011005477A1 (en) | 2009-06-22 | 2011-01-13 | Codexis, Inc. | Transaminase reactions |
| HUE038434T2 (hu) | 2010-06-17 | 2018-10-29 | Codexis Inc | Biokatalizátorok és módszerek (S)-3(1-aminoetil)-fenol szintéziséhez |
| SG11201504752YA (en) | 2012-12-21 | 2015-07-30 | Codexis Inc | Engineered biocatalysts and methods for synthesizing chiral amines |
| US9617573B2 (en) | 2013-02-28 | 2017-04-11 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
| CN106636227B (zh) * | 2016-09-22 | 2020-11-06 | 精晶药业股份有限公司 | 一种酶法转化l-丙氨酸制备丙酮酸的方法 |
| US9692784B1 (en) * | 2016-10-25 | 2017-06-27 | Fortress Cyber Security, LLC | Security appliance |
| EP3638688A4 (en) | 2017-06-14 | 2021-07-07 | Codexis, Inc. | MODIFIED TRANSAMINASE POLYPEPTIDES INTENDED FOR INDUSTRIAL BIOCATALYSIS |
| CN107586796B (zh) * | 2017-07-20 | 2021-08-06 | 暨明医药科技（苏州）有限公司 | (r)-2-(1-氨基乙基)-4-氟苯酚的合成方法 |
| CA3126671A1 (en) | 2019-01-15 | 2020-07-23 | Codexis, Inc. | Engineered transaminase polypeptides |
| EP4133064A4 (en) | 2020-04-10 | 2024-07-24 | Codexis, Inc. | Engineered transaminase polypeptides |
| CN111825563A (zh) * | 2020-07-01 | 2020-10-27 | 中国科学院福建物质结构研究所 | 一种β-高谷氨酸的制备方法 |
| CN115851540B (zh) | 2022-12-13 | 2023-06-06 | 广州大学 | 具有耐盐特性的异养硝化好氧反硝化脱氮除磷菌株及应用 |
| US12534008B2 (en) * | 2024-07-08 | 2026-01-27 | Hanghui Wu | Tray-table storage device |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4600692A (en) * | 1983-02-10 | 1986-07-15 | Purification Engineering, Inc. | Immobilized cells for preparing phenylalanine |
| US4518692A (en) * | 1983-09-01 | 1985-05-21 | Genetics Institute, Inc. | Production of L-amino acids by transamination |
| US4826766A (en) * | 1985-09-23 | 1989-05-02 | Genetics Institute, Inc. | Production of amino acids using coupled aminotransferases |
| US5316943A (en) * | 1988-06-14 | 1994-05-31 | Kidman Gene E | Racemic conversion of using a transaminase |
| US5169780A (en) * | 1989-06-22 | 1992-12-08 | Celgene Corporation | Enantiomeric enrichment and stereoselective synthesis of chiral amines |
| US4950606A (en) * | 1989-06-22 | 1990-08-21 | Celgene Corporation | Enantiomeric enrichment and stereoselective synthesis of chiral amines |
| US5300437A (en) * | 1989-06-22 | 1994-04-05 | Celgene Corporation | Enantiomeric enrichment and stereoselective synthesis of chiral amines |
| JPH08302117A (ja) * | 1995-05-08 | 1996-11-19 | Idemitsu Kosan Co Ltd | ポリスチレン系樹脂組成物 |
| CA2287171A1 (en) * | 1997-05-01 | 1998-11-12 | G.D. Searle & Co. | Method and apparatus for preparation of chiral beta amino acids |
| US6197558B1 (en) * | 1997-05-19 | 2001-03-06 | Nsc Technologies | Transaminase biotransformation process |
| DE60013939T2 (de) * | 1999-03-19 | 2005-10-06 | Sumitomo Chemical Co. Ltd. | Stereoselektive Transaminase, deren kodierende Gen und deren Verwendungen |
| DE10160066A1 (de) * | 2001-12-06 | 2003-06-18 | Degussa | Amidase aus Variovorax |
-
2004
- 2004-06-22 US US10/875,161 patent/US20050009151A1/en not_active Abandoned
- 2004-06-30 JP JP2006518393A patent/JP2007526751A/ja not_active Withdrawn
- 2004-06-30 WO PCT/IB2004/002183 patent/WO2005005633A2/en not_active Ceased
- 2004-06-30 MX MXPA06000367A patent/MXPA06000367A/es unknown
- 2004-06-30 CA CA002529509A patent/CA2529509A1/en not_active Abandoned
- 2004-06-30 BR BRPI0412308-5A patent/BRPI0412308A/pt not_active IP Right Cessation
- 2004-06-30 EP EP04743849A patent/EP1646718A2/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1646718A2 (en) | 2006-04-19 |
| US20050009151A1 (en) | 2005-01-13 |
| WO2005005633A2 (en) | 2005-01-20 |
| WO2005005633A3 (en) | 2005-05-12 |
| CA2529509A1 (en) | 2005-01-20 |
| JP2007526751A (ja) | 2007-09-20 |
| MXPA06000367A (es) | 2006-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 4A, 5A, 6A, 7A E 8A ANUIDADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2159 DE 22/05/2012. |