BRPI0416120A - dispositivo e método de medição da fluorescência de pelo menos uma amostra em pelo menos uma cámara de amostras e dispositivo de separação - Google Patents

dispositivo e método de medição da fluorescência de pelo menos uma amostra em pelo menos uma cámara de amostras e dispositivo de separação

Info

Publication number
BRPI0416120A
BRPI0416120A BRPI0416120-3A BRPI0416120A BRPI0416120A BR PI0416120 A BRPI0416120 A BR PI0416120A BR PI0416120 A BRPI0416120 A BR PI0416120A BR PI0416120 A BRPI0416120 A BR PI0416120A
Authority
BR
Brazil
Prior art keywords
sample
sample chamber
excitation
fluorescence
chamber
Prior art date
Application number
BRPI0416120-3A
Other languages
English (en)
Inventor
Lajos Nyarsik
Wilfried Nietfeld
Hans Lehrach
Tarso Benigno Ledur Kist
Original Assignee
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft filed Critical Max Planck Gesellschaft
Publication of BRPI0416120A publication Critical patent/BRPI0416120A/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

"DISPOSITIVO E MéTODO DE MEDIçãO DA FLUORESCêNCIA DE PELO MENOS UMA AMOSTRA EM PELO MENOS UMA CáMARA DE AMOSTRAS E DISPOSITIVO DE SEPARAçãO". Um método de medir a fluorescência de pelo menos uma amostra (1) em pelo menos uma câmara da amostra (10, 11), que se estende ao longo de uma direção (x) de referência longitudinal, compreende as etapas de posicionar a amostra (1) na câmara da amostra (10, 11), irradiar a amostra (1) com luz de excitação a partir de uma fonte de luz de excitação (20) ao longo de um trajeto de luz de excitação e detectar a luz fluorescente da amostra (1) com um dispositivo detector (30) ao longo de um trajeto de luz de detecção, em que um dos trajetos de luz de excitação e detecção se estende através da câmara da amostra (10, 11) paralelo à direção (x) de referência longitudinal e o respectivo outro trajeto de luz de excitação e detecção se estende numa direção (y) desviando-se da direção (x) de referência longitudinal. Além disso, é descrito um dispositivo para medir a fluorescência, de acordo com este método.
BRPI0416120-3A 2003-11-05 2004-10-29 dispositivo e método de medição da fluorescência de pelo menos uma amostra em pelo menos uma cámara de amostras e dispositivo de separação BRPI0416120A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03025455 2003-11-05
PCT/EP2004/012300 WO2005050187A1 (en) 2003-11-05 2004-10-29 Methods and devices for measuring fluorescence of a sample in a capillary

Publications (1)

Publication Number Publication Date
BRPI0416120A true BRPI0416120A (pt) 2007-01-02

Family

ID=34610044

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0416120-3A BRPI0416120A (pt) 2003-11-05 2004-10-29 dispositivo e método de medição da fluorescência de pelo menos uma amostra em pelo menos uma cámara de amostras e dispositivo de separação

Country Status (3)

Country Link
EP (1) EP1682883A1 (pt)
BR (1) BRPI0416120A (pt)
WO (1) WO2005050187A1 (pt)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104512A (en) * 1990-05-14 1992-04-14 Labintelligence, Inc. Gel electrophoresis system
US5184192A (en) * 1991-07-17 1993-02-02 Millipore Corporation Photometric apparatus with a flow cell coated with an amorphous fluoropolymer
US5784154A (en) * 1992-01-13 1998-07-21 Anthony R. Torres Electrophoresis separation in a capillary passage
JPH10239278A (ja) * 1997-02-24 1998-09-11 Hitachi Ltd 電気泳動装置
FR2774472B1 (fr) * 1998-01-30 2000-04-21 Centre Nat Rech Scient Perfectionnements aux systemes d'electrophorese multicapillaire
DE60220497T2 (de) * 2001-01-26 2008-01-31 Biocal Technology, Inc., Irvine Optische detektion in einem mehrkanaligen bioseparationssystem
FR2827958B1 (fr) * 2001-07-25 2003-09-26 Picometrics Dispositif d'analyse par fluorescence induite par laser et appareil de separation avec un tel dispositif

Also Published As

Publication number Publication date
EP1682883A1 (en) 2006-07-26
WO2005050187A1 (en) 2005-06-02

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired