BRPI0512143A - plasmìdeo mob- derivado de rsf1010, bactéria, e, método para produzir um metabólito útil - Google Patents

plasmìdeo mob- derivado de rsf1010, bactéria, e, método para produzir um metabólito útil

Info

Publication number
BRPI0512143A
BRPI0512143A BRPI0512143-4A BRPI0512143A BRPI0512143A BR PI0512143 A BRPI0512143 A BR PI0512143A BR PI0512143 A BRPI0512143 A BR PI0512143A BR PI0512143 A BRPI0512143 A BR PI0512143A
Authority
BR
Brazil
Prior art keywords
mob
plasmid
rsf1010
producing
bacterium
Prior art date
Application number
BRPI0512143-4A
Other languages
English (en)
Inventor
Joanna Yosifovna Katashkina
Aleksandra Yuriev Skorokhodova
Danila Vadimovich Zimenkov
Andrey Yurievich Gulevich
Lopes Lubov Errais
Irina Vladimirovna Biryukova
Aleksandr Sergeevich Mirovov
Sergei Vladimirovich Mashko
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BRPI0512143A publication Critical patent/BRPI0512143A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PLASMìDEO MOB DERIVADO DE RSF1010, BACTéRIA, E, MéTODO PARA PRODUZIR UM METABóLITO úTIL Um plasmídeo Mob tendo um replicon RSF1010, que compreende um gene que codifica a proteína Rep e o dito plasmídeo foi modificado para inativar o gene relacionado com a capacidade de mobilização. A presente invenção também descreve uma bactéria tendo uma capacidade para produzir metabólitos úteis, que compreende o plasmídeo e a dita bactéria carece da timidilato sintase ativa codificada pelo gene thyA e timidina quinase codificada pelo gene tdk e um método para produzir metabólitos úteis, tal como proteínas nativas ou recombinante, enzimas, L-aminoácidos, nucleosídeos e nucleotídeos, ácido orgânico, vitaminas, usando a bactéria.
BRPI0512143-4A 2004-06-24 2005-06-24 plasmìdeo mob- derivado de rsf1010, bactéria, e, método para produzir um metabólito útil BRPI0512143A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2004119027/13A RU2306338C2 (ru) 2004-06-24 2004-06-24 Mob'-ПРОИЗВОДНАЯ ПЛАЗМИДА RSF1010, НЕ СОДЕРЖАЩАЯ ГЕНЫ УСТОЙЧИВОСТИ К АНТИБИОТИКАМ, БАКТЕРИЯ, СОДЕРЖАЩАЯ УКАЗАННУЮ ПЛАЗМИДУ, И СПОСОБ ПОЛУЧЕНИЯ ПОЛЕЗНЫХ МЕТАБОЛИТОВ
PCT/JP2005/012159 WO2006001514A1 (en) 2004-06-24 2005-06-24 Rsf1010 derivative mob-deficient plasmid containing no antibiotic resistance gene, bacterium comprising the plasmid and method for producing useful metabolites

Publications (1)

Publication Number Publication Date
BRPI0512143A true BRPI0512143A (pt) 2008-02-12

Family

ID=34971535

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0512143-4A BRPI0512143A (pt) 2004-06-24 2005-06-24 plasmìdeo mob- derivado de rsf1010, bactéria, e, método para produzir um metabólito útil

Country Status (7)

Country Link
US (1) US20060014257A1 (pt)
EP (1) EP1761632A1 (pt)
JP (1) JP2008503202A (pt)
CN (1) CN1973043A (pt)
BR (1) BRPI0512143A (pt)
RU (1) RU2306338C2 (pt)
WO (1) WO2006001514A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2229513C2 (ru) * 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты)
GB0207021D0 (en) * 2002-03-25 2002-05-08 Univ Warwick Anti-bacterial agents
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
US7915018B2 (en) 2004-10-22 2011-03-29 Ajinomoto Co., Inc. Method for producing L-amino acids using bacteria of the Enterobacteriaceae family
ATE417119T1 (de) 2005-02-18 2008-12-15 Ajinomoto Kk Verfahren zur herstellung einer nichtaromatischen l-aminosäure unter verwendung eines bakteriums der familie enterobacteriaceae mit abgeschwächter expression des csra-gens
WO2006088235A1 (en) 2005-02-18 2006-08-24 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family
WO2007013639A1 (en) * 2005-07-25 2007-02-01 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE cpxR GENE
ATE420969T1 (de) * 2005-08-09 2009-01-15 Ajinomoto Kk Verfahren zur herstellung einer l-aminosäure unter verwendung eines bakteriums der familie enterobacteriaceae mit abgeschwächter expression des ybiv-gens
WO2007119574A2 (en) * 2006-03-23 2007-10-25 Ajinomoto Co., Inc. A method for producing an l-amino acid using bacterium of the enterobacteriaceae family with attenuated expression of a gene coding for small rna
WO2007119890A1 (en) 2006-04-18 2007-10-25 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE
RU2006129690A (ru) 2006-08-16 2008-02-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ
US8318481B2 (en) * 2007-12-07 2012-11-27 Pfenex Inc. High copy number self-replicating plasmids in pseudomonas
RU2008105793A (ru) * 2008-02-19 2009-08-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Способ конструирования оперонов, содержащих трансляционно сопряженные гены, бактерия, содержащая такой оперон, способ продукции полезного метаболита и способ мониторинга экспрессии гена
EP3569710A1 (en) * 2018-05-16 2019-11-20 BioVersys AG Bacterial vectors for genetic manipulation of bacteria
EP3947696A1 (en) 2019-03-28 2022-02-09 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
CN111471685B (zh) * 2020-05-21 2021-12-21 中国科学院水生生物研究所 一种喇叭虫rna干扰表达载体与构建方法及其应用
CN115109791B (zh) * 2022-06-22 2023-09-01 华南农业大学 一种基于IncQ型质粒泛宿主的功能基因递送载体、构建方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4912046A (en) * 1983-06-27 1990-03-27 Genentech, Inc. Portable inducible control system
IE892131A1 (en) * 1989-06-30 1991-01-02 Univ Cork Marker genes for genetic manipulation
US5670343A (en) * 1990-04-24 1997-09-23 Rhone Poulenc Biochimie Cloning and/or expression vectors, preparation method and their use
US7279313B2 (en) * 1995-09-15 2007-10-09 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
US6916646B1 (en) * 1997-06-23 2005-07-12 Genencor International, Inc. Enterobacteriaceae fermentation strains
AU746542B2 (en) * 1998-03-18 2002-05-02 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
RU2212447C2 (ru) * 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты)
RU2229513C2 (ru) * 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты)
JPWO2004074476A1 (ja) * 2003-02-21 2006-06-01 株式会社カネカ 新規ベクター
US20050191684A1 (en) * 2004-02-25 2005-09-01 Zimenkov Danila V. Method for producing L-amino acids

Also Published As

Publication number Publication date
RU2306338C2 (ru) 2007-09-20
US20060014257A1 (en) 2006-01-19
CN1973043A (zh) 2007-05-30
JP2008503202A (ja) 2008-02-07
EP1761632A1 (en) 2007-03-14
RU2004119027A (ru) 2006-01-10
WO2006001514A1 (en) 2006-01-05

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2158 DE 15/05/2012.