BRPI0513691A - glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido - Google Patents

glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido

Info

Publication number
BRPI0513691A
BRPI0513691A BRPI0513691-1A BRPI0513691A BRPI0513691A BR PI0513691 A BRPI0513691 A BR PI0513691A BR PI0513691 A BRPI0513691 A BR PI0513691A BR PI0513691 A BRPI0513691 A BR PI0513691A
Authority
BR
Brazil
Prior art keywords
dependent
amino acid
quinone
gdh
pyrroloquinoline
Prior art date
Application number
BRPI0513691-1A
Other languages
English (en)
Inventor
Mara Boenitz-Dulat
Jessica Laggerbauer
Rainer Schmuck
Peter Kratzsch
Wolfgang-Reinhold Knappe
Original Assignee
Roche Diagnostics Gmbh
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Hoffmann La Roche filed Critical Roche Diagnostics Gmbh
Publication of BRPI0513691A publication Critical patent/BRPI0513691A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

GLICOSE DESIDROGENASE DEPENDENTE DE PIRROLOQUINOLINA QUINONA TRANSFORMADA GENETICAMENTE COMPREENDENDO UMA INSERçãO DE AMINOáCIDO. A presente invenção refere-se às variantes aperfeiçoadas de glicose desidrogenases solúveis dependente de pirroloquinolina quinona (PQQ) (s-GDH) compreendendo uma inserção de aminoácido entre as posições 428 e 429 como correspondendo á seqüência de aminoácido conhecida do Acinetobacter calcoacetícus, aos genes codificando tal variante de s-GDH, às proteínas de tais variantes de s-GDH com especificidade de substrato aperfeiçoada para glicose, e ás diferentes aplicações dessas variantes de s-GDH, especificamente para determinação de concentrações de açúcar, especialmente de glicose em uma amostra.
BRPI0513691-1A 2004-07-20 2005-07-19 glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido BRPI0513691A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04017069 2004-07-20
PCT/EP2005/007844 WO2006008132A1 (en) 2004-07-20 2005-07-19 Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Publications (1)

Publication Number Publication Date
BRPI0513691A true BRPI0513691A (pt) 2008-03-25

Family

ID=34925823

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0513691-1A BRPI0513691A (pt) 2004-07-20 2005-07-19 glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido

Country Status (15)

Country Link
US (1) US7547524B2 (pt)
EP (2) EP1771553A1 (pt)
JP (1) JP4842938B2 (pt)
KR (1) KR100879491B1 (pt)
CN (1) CN100549167C (pt)
AU (1) AU2005263591B2 (pt)
BR (1) BRPI0513691A (pt)
CA (1) CA2570593C (pt)
IL (1) IL179750A (pt)
MX (1) MX2007000560A (pt)
NO (1) NO340499B1 (pt)
NZ (1) NZ551870A (pt)
RU (1) RU2336306C1 (pt)
WO (1) WO2006008132A1 (pt)
ZA (1) ZA200700544B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2010649T3 (pl) 2006-04-13 2017-08-31 F. Hoffmann-La Roche Ag Ulepszone mutanty rozpuszczalnej dehydrogenazy glukozowej zależnej od chinonu pirolochinoliny
EP2329255A4 (en) 2008-08-27 2014-04-09 Edwards Lifesciences Corp analyte
FR2948680B1 (fr) 2009-07-28 2013-10-04 Centre Nat Rech Scient Nouveaux mutants de la pqq s-gdh
US9234239B2 (en) * 2009-10-23 2016-01-12 Life Technologies Corporation Systems and methods for error correction in DNA sequencing
US20130092536A1 (en) 2010-07-23 2013-04-18 Roche Diagnostics Operations, Inc. Zwitterion buffer containing compositions and uses in electroanalytical methods
CA2822331A1 (en) 2010-12-20 2012-07-19 Lek Pharmaceuticals D.D. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP3901624B1 (en) 2010-12-22 2024-01-31 Roche Diabetes Care GmbH Methods to compensate for sources of error during electrochemical testing
CN105331591B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) * 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) * 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
US5902731A (en) * 1998-09-28 1999-05-11 Lifescan, Inc. Diagnostics based on tetrazolium compounds
JP2000350588A (ja) * 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) * 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
EP1332216B1 (en) * 2000-10-27 2011-08-17 Roche Diagnostics GmbH Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
US6507120B2 (en) * 2000-12-22 2003-01-14 Siliconware Precision Industries Co., Ltd. Flip chip type quad flat non-leaded package
EP1367120A3 (en) * 2002-05-27 2004-06-02 Toyo Boseki Kabushiki Kaisha Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素

Also Published As

Publication number Publication date
CA2570593C (en) 2012-03-20
WO2006008132A1 (en) 2006-01-26
ZA200700544B (en) 2008-10-29
JP4842938B2 (ja) 2011-12-21
EP1771553A1 (en) 2007-04-11
CN100549167C (zh) 2009-10-14
IL179750A (en) 2015-11-30
EP3521421A1 (en) 2019-08-07
NO340499B1 (no) 2017-05-02
IL179750A0 (en) 2007-05-15
KR20070032794A (ko) 2007-03-22
AU2005263591A1 (en) 2006-01-26
CA2570593A1 (en) 2006-01-26
US20070243566A1 (en) 2007-10-18
US7547524B2 (en) 2009-06-16
JP2008506375A (ja) 2008-03-06
NO20070946L (no) 2007-04-11
NZ551870A (en) 2009-02-28
RU2336306C1 (ru) 2008-10-20
KR100879491B1 (ko) 2009-01-20
CN1989241A (zh) 2007-06-27
MX2007000560A (es) 2007-03-07
AU2005263591B2 (en) 2008-08-14
HK1107829A1 (zh) 2008-04-18

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11N Dismissal: publication cancelled [chapter 11.14 patent gazette]

Free format text: REFERENTE A RPI 2049 DE 13/04/2010.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]

Free format text: NOTIFICACAO DE DEVOLUCAO DO PEDIDO POR NAO SE ENQUADRAR NO ART. 229-C DA LPI.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]