BRPI0514778A - meio e cultivo das células tronco embriÈnica - Google Patents

meio e cultivo das células tronco embriÈnica

Info

Publication number
BRPI0514778A
BRPI0514778A BRPI0514778-6A BRPI0514778A BRPI0514778A BR PI0514778 A BRPI0514778 A BR PI0514778A BR PI0514778 A BRPI0514778 A BR PI0514778A BR PI0514778 A BRPI0514778 A BR PI0514778A
Authority
BR
Brazil
Prior art keywords
cells
embryonic stem
medium
fibroblast
stem cell
Prior art date
Application number
BRPI0514778-6A
Other languages
English (en)
Inventor
James A Thomson
Tenneille Ludwing
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BRPI0514778A publication Critical patent/BRPI0514778A/pt
Publication of BRPI0514778B1 publication Critical patent/BRPI0514778B1/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
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    • C12N2500/32—Amino acids
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    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MEIO E CULTIVO DAS CéLULAS TRONCO EMBRIÈNICA. Os métodos prévios para cultivação das células tronco embriónica humana tem requerido ou células alimentadoras do fibroblasto ou um meio, que tem sido exposto às células aumentadoras do fibroblasto, a fim de manter as células tronco em um estado não diferenciado. Agora tem agora sido constatado que se os níveis elevados do fator de crescimento do fibroblasto, ácido gama amino butírico, ácido pipecólico, lítio e transformando o fator de crescimento beta são adicionados ao meio em que as células são cultuvadas, as células tronco permanecerão indefinidamente não diferenciadas através das múltiplas passagens, ainda sem células alimentadoras ou meio de condicionado.
BRPI0514778-6A 2004-09-08 2005-09-08 Meio para cultivar células tronco embrionárias BRPI0514778B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
US60/608,040 2004-09-08
PCT/US2005/031838 WO2006029197A1 (en) 2004-09-08 2005-09-08 Medium and culture of embryonic stem cells

Publications (2)

Publication Number Publication Date
BRPI0514778A true BRPI0514778A (pt) 2008-06-24
BRPI0514778B1 BRPI0514778B1 (pt) 2018-07-10

Family

ID=35351611

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514778-6A BRPI0514778B1 (pt) 2004-09-08 2005-09-08 Meio para cultivar células tronco embrionárias

Country Status (17)

Country Link
US (5) US7449334B2 (pt)
EP (1) EP1791948B1 (pt)
JP (3) JP5420837B2 (pt)
KR (1) KR101264940B1 (pt)
CN (2) CN101052712A (pt)
AT (1) ATE549395T1 (pt)
AU (1) AU2005282510B2 (pt)
BR (1) BRPI0514778B1 (pt)
CA (1) CA2579643C (pt)
ES (1) ES2383813T3 (pt)
GB (1) GB2432846B (pt)
IL (1) IL181437A (pt)
IS (1) IS8618A (pt)
MX (1) MX2007002390A (pt)
NO (1) NO344695B1 (pt)
NZ (1) NZ553241A (pt)
WO (1) WO2006029197A1 (pt)

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