BRPI0514778A - meio e cultivo das células tronco embriÈnica - Google Patents

meio e cultivo das células tronco embriÈnica

Info

Publication number
BRPI0514778A
BRPI0514778A BRPI0514778-6A BRPI0514778A BRPI0514778A BR PI0514778 A BRPI0514778 A BR PI0514778A BR PI0514778 A BRPI0514778 A BR PI0514778A BR PI0514778 A BRPI0514778 A BR PI0514778A
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BR
Brazil
Prior art keywords
cells
embryonic stem
medium
fibroblast
stem cell
Prior art date
Application number
BRPI0514778-6A
Other languages
English (en)
Inventor
James A Thomson
Tenneille Ludwing
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BRPI0514778A publication Critical patent/BRPI0514778A/pt
Publication of BRPI0514778B1 publication Critical patent/BRPI0514778B1/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
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    • C12N2500/32—Amino acids
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    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MEIO E CULTIVO DAS CéLULAS TRONCO EMBRIÈNICA. Os métodos prévios para cultivação das células tronco embriónica humana tem requerido ou células alimentadoras do fibroblasto ou um meio, que tem sido exposto às células aumentadoras do fibroblasto, a fim de manter as células tronco em um estado não diferenciado. Agora tem agora sido constatado que se os níveis elevados do fator de crescimento do fibroblasto, ácido gama amino butírico, ácido pipecólico, lítio e transformando o fator de crescimento beta são adicionados ao meio em que as células são cultuvadas, as células tronco permanecerão indefinidamente não diferenciadas através das múltiplas passagens, ainda sem células alimentadoras ou meio de condicionado.
BRPI0514778-6A 2004-09-08 2005-09-08 Meio para cultivar células tronco embrionárias BRPI0514778B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
US60/608,040 2004-09-08
PCT/US2005/031838 WO2006029197A1 (en) 2004-09-08 2005-09-08 Medium and culture of embryonic stem cells

Publications (2)

Publication Number Publication Date
BRPI0514778A true BRPI0514778A (pt) 2008-06-24
BRPI0514778B1 BRPI0514778B1 (pt) 2018-07-10

Family

ID=35351611

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514778-6A BRPI0514778B1 (pt) 2004-09-08 2005-09-08 Meio para cultivar células tronco embrionárias

Country Status (17)

Country Link
US (5) US7449334B2 (pt)
EP (1) EP1791948B1 (pt)
JP (3) JP5420837B2 (pt)
KR (1) KR101264940B1 (pt)
CN (2) CN101052712A (pt)
AT (1) ATE549395T1 (pt)
AU (1) AU2005282510B2 (pt)
BR (1) BRPI0514778B1 (pt)
CA (1) CA2579643C (pt)
ES (1) ES2383813T3 (pt)
GB (1) GB2432846B (pt)
IL (1) IL181437A (pt)
IS (1) IS8618A (pt)
MX (1) MX2007002390A (pt)
NO (1) NO344695B1 (pt)
NZ (1) NZ553241A (pt)
WO (1) WO2006029197A1 (pt)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0514778B1 (pt) * 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
DK3196296T3 (en) * 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
EP1888123B1 (en) 2005-06-08 2013-01-09 Janssen Biotech, Inc. A cellular therapy for ocular degeneration
US20070025973A1 (en) * 2005-07-29 2007-02-01 Fitzsimmons Frederick J Reprogramming of adult or neonic stem cells and methods of use
AU2006301549B2 (en) * 2005-10-07 2013-02-14 Cellartis Ab A method for obtaining a xeno-free hBS cell line
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
GB0613756D0 (en) * 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
CA2675445C (en) * 2007-01-17 2014-11-04 Wisconsin Alumni Research Foundation Improved culture of stem cells
EP2139988A1 (en) * 2007-03-15 2010-01-06 California Stem Cell, Inc. Cardiomyocytes and methods of producing and purifying cardiomyocytes
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
DK2173863T3 (en) * 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
US9096832B2 (en) 2007-07-31 2015-08-04 Lifescan, Inc. Differentiation of human embryonic stem cells
ATE523585T1 (de) 2007-11-27 2011-09-15 Lifescan Inc Differenzierung menschlicher embryonaler stammzellen
KR101574989B1 (ko) * 2007-12-28 2015-12-21 후지레비오 가부시키가이샤 포유 동물 체세포용 배지 및 이를 위한 첨가제
CA2959401C (en) 2008-02-21 2021-12-07 Centocor Ortho Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US8513012B2 (en) * 2008-05-02 2013-08-20 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells
KR101651661B1 (ko) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
EP2318516A1 (en) * 2008-06-30 2011-05-11 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
WO2010017562A2 (en) 2008-08-08 2010-02-11 Mayo Foundation For Medical Education And Research Induced pluripotent stem cells
CN102272291B (zh) 2008-10-31 2018-01-16 詹森生物科技公司 人胚胎干细胞向胰腺内分泌谱系的分化
MX2011004563A (es) 2008-10-31 2011-06-01 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
PL2366022T3 (pl) 2008-11-20 2016-11-30 Sposoby i kompozycje do przyłączania i hodowli komórek na podłożach planarnych
PL2366021T3 (pl) 2008-11-20 2017-12-29 Janssen Biotech, Inc. Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach
EP2398897B1 (en) 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
CN102388130B (zh) 2009-02-27 2014-08-27 细胞动力国际有限公司 多能细胞的分化
ES2693088T3 (es) 2009-07-20 2018-12-07 Janssen Biotech, Inc. Diferenciación de células madre embriónicas humanas
AU2010276402B2 (en) 2009-07-20 2014-07-03 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
KR20170118969A (ko) 2009-07-20 2017-10-25 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
CN102695790A (zh) * 2009-10-29 2012-09-26 詹森生物科技公司 多能干细胞
EP2501802A4 (en) 2009-11-17 2013-08-21 Advanced Cell Tech Inc METHOD FOR PRODUCING HUMAN RPE CELLS AND PHARMACEUTICAL PREPARATIONS FROM HUMAN RPE CELLS
FI20096288A0 (fi) * 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
AU2010333839C1 (en) 2009-12-23 2016-07-21 Janssen Biotech Inc. Differentiation of human embryonic stem cells
MX339669B (es) 2009-12-23 2016-06-02 Janssen Biotech Inc Diferenciacion de celulas madres embrionarias humanas.
CN107189979B (zh) 2010-03-01 2021-05-04 詹森生物科技公司 纯化衍生自多能干细胞的细胞的方法
KR101986176B1 (ko) 2010-05-12 2019-06-05 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
AU2010355614B2 (en) 2010-06-13 2015-07-02 Institute Of Biophysics, Chinese Academy Of Sciences Methods and compositions for preparing cardiomyocytes from stem cells and uses thereof
AU2011280878B2 (en) 2010-07-23 2016-06-16 Astellas Institute For Regenerative Medicine Methods for detection of rare subpopulations of cells and highly purified compositions of cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
CN103180434A (zh) 2010-08-05 2013-06-26 威斯康星校友研究基金会 用于人多能细胞培养的简化基础培养基
WO2012030540A2 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
WO2012030539A2 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
AU2011296381B2 (en) 2010-08-31 2016-03-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN102559587A (zh) * 2010-12-16 2012-07-11 中国科学院上海药物研究所 诱导性多能干细胞的制备方法以及用于制备诱导性多能干细胞的培养基
JP6002675B2 (ja) * 2010-12-17 2016-10-05 ビオラミナ アーベー 細胞培養培地
US9133266B2 (en) * 2011-05-06 2015-09-15 Wisconsin Alumni Research Foundation Vitronectin-derived cell culture substrate and uses thereof
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
WO2013022584A1 (en) 2011-08-05 2013-02-14 Corning Incorporated Modulation of the mammalian receptor mtor to inhibit stem cell differentiation into neurons
CN102399742B (zh) * 2011-09-30 2014-07-30 东莞中山大学研究院 用于哺乳动物唾液腺上皮细胞和唾液腺干细胞培养的无血清培养液
US10238755B2 (en) 2011-11-30 2019-03-26 The Wistar Institute Of Anatomy And Biology Methods and compositions for regulation of cell aging, carcinogenesis and reprogramming
RU2691027C2 (ru) 2011-12-05 2019-06-07 Фэктор Байосайенс Инк. Способы и препараты для трансфекции клеток
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
US8772460B2 (en) 2011-12-16 2014-07-08 Wisconsin Alumni Research Foundation Thermostable FGF-2 mutant having enhanced stability
US9388386B2 (en) 2011-12-22 2016-07-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
CA2866590A1 (en) 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
US9260696B2 (en) 2012-04-24 2016-02-16 Dan S. Kaufman Method for developing natural killer cells from stem cells
WO2013184888A1 (en) 2012-06-08 2013-12-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
EP2891712B1 (en) 2012-07-23 2026-01-28 Institute Of Biophysics, Chinese Academy Of Sciences Method for inducing pluripotent stem cells to differentiate into ventricular myocytes in vitro
EP2914728B1 (en) 2012-11-01 2020-07-08 Factor Bioscience Inc. Methods and products for expressing proteins in cells
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
WO2014105543A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
RU2684215C2 (ru) 2012-12-31 2019-04-04 Янссен Байотек, Инк. Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток
SG11201507275TA (en) 2013-03-13 2015-10-29 Wisconsin Alumni Res Found Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
NL2011170C2 (en) * 2013-07-15 2015-01-21 Univ Erasmus Medical Ct Method and culture medium for in vitro culturing of stem cells.
US20160230143A1 (en) 2013-09-19 2016-08-11 The U.S.A., As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CN105940110A (zh) 2014-01-31 2016-09-14 菲克特生物科学股份有限公司 用于核酸产生和递送的方法和产品
WO2015164740A1 (en) 2014-04-24 2015-10-29 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
US10280400B2 (en) 2014-05-05 2019-05-07 Indiana University Research And Technology Corporation Method for producing retinal ganglion cells from pluripotent cells
JP6588969B2 (ja) 2014-05-16 2019-10-09 ヤンセン バイオテツク，インコーポレーテツド 膵内分泌細胞内のｍａｆａ発現を強化するための小分子の使用
WO2016131052A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
CN104830772A (zh) * 2015-05-28 2015-08-12 深圳富利鑫健康产业发展有限公司 一种造血干细胞培养基及其应用和干细胞培养方法
ES2862676T3 (es) * 2015-10-20 2021-10-07 Fujifilm Cellular Dynamics Inc Producción de células precursoras hematopoyéticas multilinaje mediante programación genética
JP6705911B2 (ja) 2016-04-14 2020-06-03 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の中腸内胚葉細胞への分化
IL308824A (en) 2016-08-17 2024-01-01 Factor Bioscience Inc Nucleic acid products and methods of administration thereof
CA3038721A1 (en) 2016-09-30 2018-04-05 Mayo Foundation For Medical Education And Research Viral vectors for nuclear reprogramming
JP7049347B2 (ja) * 2017-01-23 2022-04-06 ステムセル テクノロジーズ カナダ インコーポレイテッド 幹細胞の生存および増殖を高めるための培地および方法
AU2018254442B2 (en) 2017-04-18 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
CA3064864A1 (en) * 2017-05-25 2018-11-29 New York Stem Cell Foundation, Inc. Method and composition for generating basal forebrain cholinergic neurons (bfcns)
CA3068685A1 (en) 2017-06-27 2019-01-03 Ajinomoto Co., Inc. Riboflavin derivative-containing medium
KR102121042B1 (ko) * 2017-12-06 2020-06-09 이정복 조류의 에그로부터 분리한 세포인 배반엽 전분화능세포, 신경줄기세포, 배아섬유아세포 컨디션드 배지를 유효성분으로 하는 세포 증식, 피부재생 및 주름 개선용 배지 조성물
WO2019177420A1 (ko) * 2018-03-16 2019-09-19 사회복지법인 삼성생명공익재단 Fgf-17을 유효 성분으로 포함하는 줄기세포 증식 촉진용 배지 조성물 및 이를 이용한 줄기세포 증식 촉진 방법
KR102216302B1 (ko) * 2018-09-21 2021-02-17 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도
JP7699552B2 (ja) 2019-07-03 2025-06-27 ファクター バイオサイエンス インコーポレイテッド カチオン性脂質及びその使用
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
CN111690595B (zh) * 2020-07-21 2021-07-16 生物岛实验室 一种无血清无饲养层的胚胎干细胞或多能干细胞培养基及其应用
CN112029708A (zh) * 2020-09-09 2020-12-04 江苏育瑞康生物科技有限公司 一种干细胞培养系统

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3880795B2 (ja) 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
EP1572984B8 (en) * 2002-12-16 2016-06-01 Technion Research & Development Foundation Ltd. Feeder-free, xeno-free culture system for human embryonic stem cells
KR100632851B1 (ko) 2004-01-20 2006-10-13 한국원자력연구소 전분화능 줄기세포의 분화를 억제하는 방법
JP5128946B2 (ja) * 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
BRPI0514778B1 (pt) * 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
DK3196296T3 (en) * 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells

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