BRPI0606612A2 - expressão recombinante de proteìnas em uma forma de duas cadeias ligadas por dissulfeto - Google Patents

expressão recombinante de proteìnas em uma forma de duas cadeias ligadas por dissulfeto

Info

Publication number
BRPI0606612A2
BRPI0606612A2 BRPI0606612-7A BRPI0606612A BRPI0606612A2 BR PI0606612 A2 BRPI0606612 A2 BR PI0606612A2 BR PI0606612 A BRPI0606612 A BR PI0606612A BR PI0606612 A2 BRPI0606612 A2 BR PI0606612A2
Authority
BR
Brazil
Prior art keywords
dichain
ile
phe
leu
val
Prior art date
Application number
BRPI0606612-7A
Other languages
English (en)
Inventor
Volker Specht
Original Assignee
Biotecon Therapeutics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotecon Therapeutics Gmbh filed Critical Biotecon Therapeutics Gmbh
Publication of BRPI0606612A2 publication Critical patent/BRPI0606612A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

EXPRESSAO RECOMBINANTE DE PROTEìNAS EM UMA FORMA DE DUAS CADEIAS LIGADAS POR DISSULFETO. A invenção se relaciona a um método para produzir polipeptideos ou proteínas em uma forma de duas cadeias por expressão recombinante em células hospedeiras de E. coli, em que i) o polipeptideo ou proteína exerce sua atividade biológica como um polipeptideo ou proteína de duas cadeias, ii) o grupo de aminoácido C-terminal da primeira cadeia é um grupo Arg ou Lys, iii) a segunda cadeia da proteina/polipeptideo, em sua extremidade N-terminal, tem 1 até 20 grupos de aminoácidos e uma seqUência pentapeptídica VPXGS referida como PRS, em que X pode ser qualquer aminoácido que ocorre naturalmente, em que V representa Val, Leu, Ile, Ala, Phe, Pro ou Gly, P representa Pro, Leu, Ile, Ala, Phe, Val ou Gly, O representa Gly, Leu, Ile, Ala, Pro, Phe ou Val e 5 representa Ser, Tyr, Trp ou Thr; e (iv) o método compreende as seguintes etapas: a) modificar o polipeptídeo ou proteína no nível de ácido nucléico de tal maneira que o polipeptideo ou proteína na sua forma modificada compreende a seqUência VPXGS em sua área de alça, com X, V, P. O e 5 definidos como acima; b) introduzir a construção modificada no nível de ácido nucléico em células de E. coli; c) cultivo e então lise das células hospedeiras; e d) isolar o polipeptideo ou proteína de duas cadeias. De acordo com uma modalidade preferida da invenção, o polipeptídeo ou proteína é uma neurotoxina botulínica, especialmente a neurotoxina botulínica (BoNT(A)) tipo A.
BRPI0606612-7A 2005-01-21 2006-01-20 expressão recombinante de proteìnas em uma forma de duas cadeias ligadas por dissulfeto BRPI0606612A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005002978A DE102005002978B4 (de) 2005-01-21 2005-01-21 Rekombinante Expression von Proteinen in einer disulfidverbrückten, zweikettigen Form
PCT/DE2006/000088 WO2006076902A2 (de) 2005-01-21 2006-01-20 Rekombinante expression von proteinen in einer disulfidverbrückten, zweikettigen form

Publications (1)

Publication Number Publication Date
BRPI0606612A2 true BRPI0606612A2 (pt) 2009-07-07

Family

ID=36603464

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0606612-7A BRPI0606612A2 (pt) 2005-01-21 2006-01-20 expressão recombinante de proteìnas em uma forma de duas cadeias ligadas por dissulfeto

Country Status (15)

Country Link
US (1) US20080103098A1 (pt)
EP (1) EP1844150B1 (pt)
JP (2) JP5107055B2 (pt)
KR (1) KR20070104605A (pt)
CN (2) CN101107361B (pt)
AT (1) ATE546533T1 (pt)
AU (1) AU2006207726B2 (pt)
BR (1) BRPI0606612A2 (pt)
CA (1) CA2593520C (pt)
DE (2) DE102005002978B4 (pt)
DK (1) DK1844150T3 (pt)
ES (1) ES2380677T3 (pt)
IL (1) IL184112A0 (pt)
RU (1) RU2412253C2 (pt)
WO (1) WO2006076902A2 (pt)

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EP2218783A1 (en) 2009-02-05 2010-08-18 Merz Pharma GmbH & Co. KGaA Novel method for the manufacturing of neurotoxins
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CN111454931A (zh) * 2012-11-21 2020-07-28 益普生生物创新有限公司 用于制备经蛋白水解处理的多肽的方法
EP3113792B1 (en) 2014-03-05 2018-12-05 Merz Pharma GmbH & Co. KGaA Novel recombinant clostridial neurotoxins with increased duration of effect
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TWI799366B (zh) 2015-09-15 2023-04-21 美商建南德克公司 胱胺酸結骨架平臺
ES2929347T3 (es) 2016-01-20 2022-11-28 Merz Pharma Gmbh & Co Kgaa Nuevas neurotoxinas clostridiales recombinantes con aumento de la duración del efecto
ES2893838T3 (es) 2016-03-02 2022-02-10 Merz Pharma Gmbh & Co Kgaa Composición que comprende toxina botulínica
EP3263710A1 (en) 2016-07-01 2018-01-03 Ipsen Biopharm Limited Production of activated clostridial neurotoxins
EP3290437A1 (en) 2016-08-31 2018-03-07 Merz Pharma GmbH & Co. KGaA Novel recombinant clostridial neurotoxins with decreased duration of effect
EP3312193A1 (en) 2016-10-19 2018-04-25 Merz Pharma GmbH & Co. KGaA Novel recombinant botulinum neurotoxins with accelerated onset of effect
EP3333179A1 (en) 2016-12-07 2018-06-13 Merz Pharma GmbH & Co. KGaA Novel recombinant botulinum toxin with accelarated onset of effect
EP3335719A1 (en) 2016-12-14 2018-06-20 Merz Pharma GmbH & Co. KGaA Novel recombinant botulinum neurotoxins with a stabilized light chain
WO2018233813A1 (en) 2017-06-20 2018-12-27 Merz Pharma Gmbh & Co. Kgaa NOVEL RECOMBINANT BOTULINUM TOXINS WITH INCREASED DURATION
US11155802B2 (en) 2017-07-06 2021-10-26 Merz Pharma Gmbh & Co. Kgaa Recombinant botulinum neurotoxins with increased duration of effect
US20210008156A1 (en) 2017-10-26 2021-01-14 Merz Pharma Gmbh & Co. Kgaa Novel recombinant botulinum neurotoxins with increased duration of effect
WO2019101308A1 (en) 2017-11-22 2019-05-31 Merz Pharma Gmbh & Co. Kgaa Novel recombinant botulinum toxin with increased duration of effect
WO2019158745A1 (de) * 2018-02-16 2019-08-22 Bontana Therapies Gmbh Nukleinsäure-basiertes botulinum neurotoxin zur therapeutischen anwendung
JP2021525514A (ja) * 2018-05-30 2021-09-27 ザ ガバニング カウンシル オブ ザ ユニバーシティ オブ トロントＴｈｅ Ｇｏｖｅｒｎｉｎｇ Ｃｏｕｎｃｉｌ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｔｏｒｏｎｔｏ 受容体−リガンド相互作用に関連付けられるタンパク質を同定するための方法及びキット
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CN114269932B (zh) * 2019-12-18 2024-08-06 Mvrix株式会社 肉毒神经毒素的安全制备方法
CN114957482B (zh) * 2021-02-26 2024-09-24 重庆誉颜制药有限公司 一种经修饰的神经毒素的单链多肽及其用途
CN113480667B (zh) * 2021-08-17 2023-03-28 长春萤火虫生物科技有限公司 一种人粒细胞集落刺激因子突变体重组融合蛋白及其制备方法和应用
CN115894641B (zh) * 2022-09-13 2023-09-29 君合盟生物制药(杭州)有限公司 A型肉毒素突变体及其基因工程菌的构建
JP7760716B2 (ja) * 2022-05-24 2025-10-27 ジェイ・エイチ・エム バイオファーマシューティカル（ハンチョウ）カンパニー リミテッド 組換えａ型ボツリヌス神経毒及びその調製方法
CN115819526A (zh) * 2022-12-02 2023-03-21 海雅美生物技术(珠海)有限公司 一种重组肉毒杆菌神经毒素及其制备方法和应用
CN116813727B (zh) * 2023-06-08 2025-06-06 苏州德进生物制药有限公司 一种a型肉毒毒素的制备方法及其应用
CN118126143B (zh) * 2024-03-14 2025-06-24 河北平朴生物科技合伙企业(有限合伙) 一种a型肉毒毒素的突变体及其应用
CN121406618A (zh) * 2025-11-05 2026-01-27 中国人民解放军军事科学院军事医学研究院 一种肉毒毒素a1型重组蛋白及其制备方法和应用

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Also Published As

Publication number Publication date
DE112006000677A5 (de) 2007-12-27
IL184112A0 (en) 2007-10-31
RU2007127624A (ru) 2009-02-27
AU2006207726B2 (en) 2011-04-14
DE102005002978B4 (de) 2013-04-25
EP1844150A2 (de) 2007-10-17
RU2412253C2 (ru) 2011-02-20
CA2593520A1 (en) 2006-07-27
AU2006207726A1 (en) 2006-07-27
ATE546533T1 (de) 2012-03-15
US20080103098A1 (en) 2008-05-01
CN101107361A (zh) 2008-01-16
WO2006076902A3 (de) 2006-11-02
CA2593520C (en) 2013-11-19
DK1844150T3 (da) 2012-05-29
DE102005002978A1 (de) 2006-08-03
JP2012254088A (ja) 2012-12-27
EP1844150B1 (de) 2012-02-22
KR20070104605A (ko) 2007-10-26
ES2380677T3 (es) 2012-05-17
CN103320459A (zh) 2013-09-25
WO2006076902A2 (de) 2006-07-27
JP2008527983A (ja) 2008-07-31
CN101107361B (zh) 2013-07-17
JP5107055B2 (ja) 2012-12-26

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