BRPI0812920A2 - Sistema de expressão de celulase heteróloga e homóloga - Google Patents

Sistema de expressão de celulase heteróloga e homóloga

Info

Publication number
BRPI0812920A2
BRPI0812920A2 BRPI0812920-7A2A BRPI0812920A BRPI0812920A2 BR PI0812920 A2 BRPI0812920 A2 BR PI0812920A2 BR PI0812920 A BRPI0812920 A BR PI0812920A BR PI0812920 A2 BRPI0812920 A2 BR PI0812920A2
Authority
BR
Brazil
Prior art keywords
heterologous
expression system
cellular expression
homologous cellular
homologous
Prior art date
Application number
BRPI0812920-7A2A
Other languages
English (en)
Inventor
Benjamin S Bower
Edmund A Larenas
Original Assignee
Danisco Us Inc Genencor Div
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc Genencor Div filed Critical Danisco Us Inc Genencor Div
Publication of BRPI0812920A2 publication Critical patent/BRPI0812920A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445Beta-glucosidase (3.2.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021Beta-glucosidase (3.2.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0812920-7A2A 2007-06-08 2008-06-05 Sistema de expressão de celulase heteróloga e homóloga BRPI0812920A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93389407P 2007-06-08 2007-06-08
PCT/US2008/007077 WO2008153903A2 (en) 2007-06-08 2008-06-05 Heterologous and homologous cellulase expression system

Publications (1)

Publication Number Publication Date
BRPI0812920A2 true BRPI0812920A2 (pt) 2014-10-14

Family

ID=39767012

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0812920-7A2A BRPI0812920A2 (pt) 2007-06-08 2008-06-05 Sistema de expressão de celulase heteróloga e homóloga

Country Status (11)

Country Link
US (2) US20100323426A1 (pt)
EP (1) EP2171039A2 (pt)
JP (1) JP5651466B2 (pt)
CN (2) CN104726350A (pt)
BR (1) BRPI0812920A2 (pt)
CA (1) CA2693084A1 (pt)
EA (2) EA201300048A1 (pt)
IL (1) IL202304A (pt)
MX (2) MX344810B (pt)
UA (1) UA104279C2 (pt)
WO (1) WO2008153903A2 (pt)

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US8097445B2 (en) * 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
BRPI0812920A2 (pt) * 2007-06-08 2014-10-14 Danisco Us Inc Genencor Div Sistema de expressão de celulase heteróloga e homóloga
US9181537B2 (en) * 2009-07-03 2015-11-10 Meiji Seika Pharma Co., Ltd. Cellulase preparation comprising endoglucanases derived from two different types of microorganisms
MX353237B (es) 2009-09-23 2018-01-08 Danisco Us Inc Enzimas de glicosil hidrolasa novedosas y usos de las mismas.
RU2012131405A (ru) 2009-12-23 2014-01-27 ДАНИСКО ЮЭс ИНК. Способы улучшения эффективности одновременно протекающих реакций осахаривания и ферментации
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
US8759064B2 (en) 2010-05-14 2014-06-24 Codexis, Inc. Cellobiohydrolase variants
US20140134677A1 (en) 2011-03-17 2014-05-15 Danisco Us Inc. Method for reducing viscosity in saccharification process
CA2833583A1 (en) * 2011-04-27 2012-11-01 Codexis, Inc. Cellobiohydrolase variants
US8945903B2 (en) 2011-08-23 2015-02-03 Codexis, Inc. Cellobiohydrolase variants
CN102304540B (zh) * 2011-08-26 2013-10-09 华东理工大学 一种在里氏木霉分泌表达外源蛋白的表达设备及其应用
WO2013043317A1 (en) * 2011-09-20 2013-03-28 Codexis, Inc. Endoglucanase 1b
JP6105590B2 (ja) 2011-09-22 2017-03-29 ダニスコ・ユーエス・インク Dna含有量を減少させる内因性dnアーゼ活性
CA2871140A1 (en) 2012-05-21 2013-11-28 Danisco Us Inc. Trichoderma hydrophobin production
WO2014192647A1 (ja) * 2013-05-27 2014-12-04 独立行政法人産業技術総合研究所 培養細胞および糖液の製造方法
DK3419992T3 (da) 2016-02-22 2021-02-15 Danisco Us Inc Svampesystem til proteinproduktion med højt udbytte
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
US10428334B2 (en) * 2016-04-05 2019-10-01 Wisconsin Alumni Research Foundation Method of creating industrial streptomyces with capability to grow on cellulosic polysaccharide substrates
WO2019059404A1 (ja) * 2017-09-25 2019-03-28 味の素株式会社 タンパク質の製造法および二糖の製造法
JP2021035330A (ja) 2017-09-29 2021-03-04 Spiber株式会社 発現カセット
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes

Family Cites Families (26)

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US5536655A (en) * 1989-09-26 1996-07-16 Midwest Research Institute Gene coding for the E1 endoglucanase
US5475101A (en) * 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5677151A (en) * 1994-06-24 1997-10-14 Cornell Research Foundation, Inc. Thermostable cellulase from a thermomonospora gene
BR9611114A (pt) * 1995-10-17 1999-07-13 Rohm Enzyme Finland Oy Celulases os genes codificando-as e os usos das mesmas
FI964692A0 (fi) * 1996-11-25 1996-11-25 Primalco Ltd Foerbaettrad cellulassammansaettning foer bioefterbehandling av textilmaterial som innehaoller cellulosa
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US7074608B1 (en) * 1998-05-12 2006-07-11 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis
BRPI9911086B1 (pt) * 1998-06-10 2016-08-02 Novozymes As composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase
US6358715B1 (en) * 1998-12-04 2002-03-19 Genencor International, Inc. Production of ascorbic acid
CA2422558C (en) * 2000-09-25 2012-02-14 Iogen Energy Corporation Method for glucose production with a modified cellulase mixture
DK2305801T3 (en) * 2002-08-16 2017-10-09 Danisco Us Inc Hitherto UNKNOWN HYPOCREA JECORINA-CBH1 CELLULASES
US7279321B2 (en) * 2003-01-17 2007-10-09 E.I. Du Pont De Nemours And Company Methods for increasing the resistance of a host cell to aromatic carboxylic acids
WO2004078919A2 (en) * 2003-02-27 2004-09-16 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
CA2518580A1 (en) * 2003-03-12 2004-09-23 Vib Vzw Improved protein secretion in eukaryotic cells
CN1898381B (zh) * 2003-03-21 2013-07-17 金克克国际有限公司 Cbh1同源物和cbh1纤维素酶变体
EP2385104A1 (en) * 2003-04-01 2011-11-09 Danisco US Inc. Variant Scytalidium thermophilium CBH1
ES2393058T3 (es) * 2003-05-02 2012-12-18 Novozymes Inc. Variantes de beta-glucosidasa
US8097445B2 (en) * 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
BRPI0509168A (pt) * 2004-03-25 2007-09-11 Iogen Bio Products Corp xilanases modificadas que exibem expressão melhorada
US7413887B2 (en) * 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
DK2292747T3 (en) * 2004-12-30 2017-04-24 Danisco Us Inc CBH2 CELLULASE VARIETIES OF HYPOCREA JECORINA
US8206970B2 (en) * 2006-05-02 2012-06-26 Butamax(Tm) Advanced Biofuels Llc Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
BRPI0812920A2 (pt) * 2007-06-08 2014-10-14 Danisco Us Inc Genencor Div Sistema de expressão de celulase heteróloga e homóloga
AU2009240505B2 (en) * 2008-04-23 2013-09-05 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
WO2012019169A1 (en) * 2010-08-06 2012-02-09 Danisco Us Inc. Production of isoprene under neutral ph conditions
BR112013018316A2 (pt) * 2010-12-22 2018-09-11 Danisco Us Inc composições e métodos para produção aprimorada de isopreno usando dois tipos de enzimas ispg

Also Published As

Publication number Publication date
EA200971142A1 (ru) 2010-04-30
EA018049B1 (ru) 2013-05-30
HK1145851A1 (en) 2011-05-06
JP5651466B2 (ja) 2015-01-14
MX344810B (es) 2017-01-09
CA2693084A1 (en) 2008-12-18
IL202304A (en) 2016-08-31
IL202304A0 (en) 2011-08-01
JP2010528655A (ja) 2010-08-26
MX2009013329A (es) 2010-02-24
CN101772570B (zh) 2015-03-18
US20100184138A1 (en) 2010-07-22
CN104726350A (zh) 2015-06-24
US20100323426A1 (en) 2010-12-23
EP2171039A2 (en) 2010-04-07
CN101772570A (zh) 2010-07-07
UA104279C2 (uk) 2014-01-27
EA201300048A1 (ru) 2013-09-30
WO2008153903A2 (en) 2008-12-18
WO2008153903A3 (en) 2009-03-05

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]