BRPI1007214A8 - Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla. - Google Patents

Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla.

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Publication number
BRPI1007214A8
BRPI1007214A8 BRPI1007214A BRPI1007214A BRPI1007214A8 BR PI1007214 A8 BRPI1007214 A8 BR PI1007214A8 BR PI1007214 A BRPI1007214 A BR PI1007214A BR PI1007214 A BRPI1007214 A BR PI1007214A BR PI1007214 A8 BRPI1007214 A8 BR PI1007214A8
Authority
BR
Brazil
Prior art keywords
nucleic acid
adapter
sequencing
construct
methods
Prior art date
Application number
BRPI1007214A
Other languages
English (en)
Inventor
Mckeown Brian
Original Assignee
Oxford Nanopore Tech Ltd
Oxford Nanopore Tech Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Oxford Nanopore Tech Ltd, Oxford Nanopore Tech Plc filed Critical Oxford Nanopore Tech Ltd
Publication of BRPI1007214A2 publication Critical patent/BRPI1007214A2/pt
Publication of BRPI1007214A8 publication Critical patent/BRPI1007214A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MÉTODO, ADAPTADOR PARA SEQUENCIAR ÁCIDOS NUCLEICOS, E, CONSTRUTO DE ÁCIDO NUCLEICO E DE ÁCIDO NUCLEICO DE FITA SIMPLES PARA USO COMO MOLDE DE SEQUENCIAMENTO. A invenção diz respeito a adaptadores para sequenciar ácidos nucleicos. Os adaptadores podem ser usados para gerar construtos de fita simples de ácido nucleico com propósitos de sequenciamento. Tais construtos podem conter tanto molde de ácido desoxirribonucleico (DNA) quanto de ácido ribonucleico (RNA) de fita dupla. A invenção diz respeito também aos construtos gerados usando os adaptadores, métodos de preparar os adaptadores e construtos, bem como métodos de sequenciar ácidos nucleicos de fita dupla.
BRPI1007214A 2009-01-30 2010-01-29 Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla. BRPI1007214A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14873709P 2009-01-30 2009-01-30
US61/148737 2009-01-30
PCT/GB2010/000160 WO2010086622A1 (en) 2009-01-30 2010-01-29 Adaptors for nucleic acid constructs in transmembrane sequencing

Publications (2)

Publication Number Publication Date
BRPI1007214A2 BRPI1007214A2 (pt) 2019-04-09
BRPI1007214A8 true BRPI1007214A8 (pt) 2022-12-20

Family

ID=42083949

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1007214A BRPI1007214A8 (pt) 2009-01-30 2010-01-29 Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla.

Country Status (9)

Country Link
US (7) US20120058468A1 (pt)
EP (1) EP2391732B1 (pt)
JP (1) JP5843614B2 (pt)
KR (1) KR101797773B1 (pt)
CN (1) CN102369298B (pt)
AU (1) AU2010209528B2 (pt)
BR (1) BRPI1007214A8 (pt)
CA (1) CA2750879C (pt)
WO (1) WO2010086622A1 (pt)

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