BRPI1008083B8 - processo para a produção de uma linhagem de células amniócitos humanas permanentes, processo para a expressão transitória de polipeptídeos e proteínas recombinantes com o emprego da referida linhagem de células, e emprego da referida linhagem de células - Google Patents
processo para a produção de uma linhagem de células amniócitos humanas permanentes, processo para a expressão transitória de polipeptídeos e proteínas recombinantes com o emprego da referida linhagem de células, e emprego da referida linhagem de célulasInfo
- Publication number
- BRPI1008083B8 BRPI1008083B8 BRPI1008083A BRPI1008083A BRPI1008083B8 BR PI1008083 B8 BRPI1008083 B8 BR PI1008083B8 BR PI1008083 A BRPI1008083 A BR PI1008083A BR PI1008083 A BRPI1008083 A BR PI1008083A BR PI1008083 B8 BRPI1008083 B8 BR PI1008083B8
- Authority
- BR
- Brazil
- Prior art keywords
- cell line
- proteins
- transient expression
- recombinant polypeptides
- production
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 210000001776 amniocyte Anatomy 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 title abstract 3
- 230000010474 transient expression Effects 0.000 title abstract 3
- 210000005260 human cell Anatomy 0.000 abstract 2
- 150000007523 nucleic acids Chemical group 0.000 abstract 2
- 101710145634 Antigen 1 Proteins 0.000 abstract 1
- 108010031111 EBV-encoded nuclear antigen 1 Proteins 0.000 abstract 1
- 101710128836 Large T antigen Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/24—Vectors characterised by the absence of particular element, e.g. selectable marker, viral origin of replication
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- Developmental Biology & Embryology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
processo para a produção de uma linhagem de células amniócitos humanas permanentes, linhagem de células humanas aminióciticas, processo para a expressão transitória de polipeptídeos e proteínas recombinantes com o emprego de uma linhagem, emprego das células e polipeptídeo ou proteína. a presente invenção se refere a uma linhagem de células humanas permanente, compreendendo as sequências de ácidos nucleicos para as funções genéticas adenovirais e1a e e1b e a sequência de ácidos nucleicos para o antígeno t grande de sv40 ou o antígeno-1 nuclear do vírus de epstein-barr (ebv) (ebna-1). a presente invenção se refere ainda a um processo para a expressão transitória de polipeptídeos e proteínas recombinantes na linhagem de células humanas permanentes.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102009003439A DE102009003439A1 (de) | 2009-02-05 | 2009-02-05 | Neue permanente humane Zelllinie |
| DE102009003439.0 | 2009-02-05 | ||
| PCT/DE2010/075012 WO2010094280A1 (de) | 2009-02-05 | 2010-02-05 | Neue permanente humane zelllinie |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI1008083A2 BRPI1008083A2 (pt) | 2016-10-11 |
| BRPI1008083B1 BRPI1008083B1 (pt) | 2020-08-18 |
| BRPI1008083B8 true BRPI1008083B8 (pt) | 2021-05-25 |
Family
ID=42316055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI1008083A BRPI1008083B8 (pt) | 2009-02-05 | 2010-02-05 | processo para a produção de uma linhagem de células amniócitos humanas permanentes, processo para a expressão transitória de polipeptídeos e proteínas recombinantes com o emprego da referida linhagem de células, e emprego da referida linhagem de células |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US9371512B2 (pt) |
| EP (2) | EP2393919B1 (pt) |
| JP (1) | JP2012516690A (pt) |
| KR (1) | KR101695349B1 (pt) |
| CN (1) | CN102369279A (pt) |
| AU (1) | AU2010214951B2 (pt) |
| BR (1) | BRPI1008083B8 (pt) |
| CA (1) | CA2751497C (pt) |
| DE (2) | DE102009003439A1 (pt) |
| DK (2) | DK2913395T3 (pt) |
| ES (2) | ES2642837T3 (pt) |
| HU (1) | HUE036707T2 (pt) |
| IL (1) | IL214421A (pt) |
| LT (1) | LT2913395T (pt) |
| PL (2) | PL2393919T3 (pt) |
| PT (2) | PT2913395T (pt) |
| RU (1) | RU2569181C2 (pt) |
| SG (1) | SG173484A1 (pt) |
| TW (1) | TW201033363A (pt) |
| WO (1) | WO2010094280A1 (pt) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9404090B2 (en) * | 2011-11-24 | 2016-08-02 | Viromed Co., Ltd. | Adenovirus producing novel cell line and the use thereof |
| WO2014115087A2 (en) * | 2013-01-22 | 2014-07-31 | Cencor Biotech Ltd. | A method for the preparation of recombinant human prothrombin and fibrinogen |
| EP3205719A1 (en) * | 2016-02-15 | 2017-08-16 | CEVEC Pharmaceuticals GmbH | Cell lines for producing recombinant glycoproteins with di-antennary n-glycans, methods using the same, and recombinant glycoproteins |
| EP3382014A1 (en) | 2017-03-29 | 2018-10-03 | CEVEC Pharmaceuticals GmbH | Recombinant glycoproteins with reduced antennary fucosylation |
| EP3736286A1 (en) | 2019-05-09 | 2020-11-11 | Biotest AG | Single chain factor viii molecule |
| AU2020342349A1 (en) | 2019-09-02 | 2022-03-03 | Biotest Ag | Factor VIII protein with increased half-life |
| EP3785726A1 (en) | 2019-09-02 | 2021-03-03 | Biotest AG | Factor viii protein with increased half-life |
| WO2021165226A1 (en) | 2020-02-17 | 2021-08-26 | Biotest Ag | Subcutaneous administration of factor viii |
| JP2024537896A (ja) | 2021-10-18 | 2024-10-16 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞 |
| AR127674A1 (es) | 2021-10-18 | 2024-02-21 | Regeneron Pharma | Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5658763A (en) * | 1993-10-25 | 1997-08-19 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from non-native DNA |
| US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US20050170463A1 (en) * | 1999-04-15 | 2005-08-04 | Abraham Bout | Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins |
| DE19955558C2 (de) | 1999-11-18 | 2003-03-20 | Stefan Kochanek | Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren |
| US6558948B1 (en) * | 1999-11-23 | 2003-05-06 | Stefan Kochanek | Permanent amniocytic cell line, its production and use for the production of gene transfer vectors |
| RU2267538C2 (ru) * | 2000-09-08 | 2006-01-10 | Айова Стейт Юниверсити Рисерч Фаундейшн, Инк. | Способ скрининга животных с повышенным уровнем численности приплода и/или предпочтительными признаками качества мяса, белок prkag3 (варианты) и кодирующая его нуклеотидная последовательность (варианты) |
| DE102005054628A1 (de) * | 2005-11-16 | 2007-05-24 | Cevec Pharmaceuticals Gmbh | Verfahren zur Herstellung von permanenten humanen Zelllinien |
-
2009
- 2009-02-05 DE DE102009003439A patent/DE102009003439A1/de not_active Ceased
-
2010
- 2010-02-05 KR KR1020117020450A patent/KR101695349B1/ko active Active
- 2010-02-05 SG SG2011055282A patent/SG173484A1/en unknown
- 2010-02-05 DK DK15000822.5T patent/DK2913395T3/en active
- 2010-02-05 AU AU2010214951A patent/AU2010214951B2/en not_active Ceased
- 2010-02-05 PL PL10721938T patent/PL2393919T3/pl unknown
- 2010-02-05 PL PL15000822T patent/PL2913395T4/pl unknown
- 2010-02-05 PT PT150008225T patent/PT2913395T/pt unknown
- 2010-02-05 WO PCT/DE2010/075012 patent/WO2010094280A1/de not_active Ceased
- 2010-02-05 ES ES15000822.5T patent/ES2642837T3/es active Active
- 2010-02-05 DE DE112010000285T patent/DE112010000285A5/de not_active Withdrawn
- 2010-02-05 HU HUE15000822A patent/HUE036707T2/hu unknown
- 2010-02-05 TW TW099103626A patent/TW201033363A/zh unknown
- 2010-02-05 BR BRPI1008083A patent/BRPI1008083B8/pt active IP Right Grant
- 2010-02-05 US US13/147,775 patent/US9371512B2/en active Active
- 2010-02-05 CA CA2751497A patent/CA2751497C/en active Active
- 2010-02-05 CN CN201080014527XA patent/CN102369279A/zh active Pending
- 2010-02-05 PT PT107219388T patent/PT2393919E/pt unknown
- 2010-02-05 JP JP2011548530A patent/JP2012516690A/ja active Pending
- 2010-02-05 RU RU2011136451/10A patent/RU2569181C2/ru active IP Right Revival
- 2010-02-05 LT LTEP15000822.5T patent/LT2913395T/lt unknown
- 2010-02-05 EP EP10721938.8A patent/EP2393919B1/de active Active
- 2010-02-05 DK DK10721938.8T patent/DK2393919T3/en active
- 2010-02-05 ES ES10721938.8T patent/ES2547700T3/es active Active
- 2010-02-05 EP EP15000822.5A patent/EP2913395B1/de active Active
-
2011
- 2011-08-02 IL IL214421A patent/IL214421A/en active IP Right Grant
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Legal Events
| Date | Code | Title | Description |
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| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
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| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B07B | Technical examination (opinion): publication cancelled [chapter 7.2 patent gazette] | ||
| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 18/08/2020, OBSERVADAS AS CONDICOES LEGAIS. |
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| B16C | Correction of notification of the grant [chapter 16.3 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 05/02/2010 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF |