CA1047918A - Methode et appareil pour le depistage et la purification des proteines et des anticorps - Google Patents

Methode et appareil pour le depistage et la purification des proteines et des anticorps

Info

Publication number: CA1047918A
Authority: CA; Canada
Prior art keywords: protein; substrate; metal; layer; antigen
Prior art date: 1973-07-30
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA204,262A

Other languages

English (en)

Other versions

CA204262S (en

Inventor

Ivar Giaever

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

General Electric Co

Original Assignee

General Electric Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1973-07-30

Filing date

1974-07-08

Publication date

1979-02-06

1974-07-08 Application filed by General Electric Co filed Critical General Electric Co

1979-02-06 Application granted granted Critical

1979-02-06 Publication of CA1047918A publication Critical patent/CA1047918A/fr

Status Expired legal-status Critical Current

Links

238000000034 method Methods 0.000 title claims abstract description 45
102000004169 proteins and genes Human genes 0.000 title abstract description 198
108090000623 proteins and genes Proteins 0.000 title abstract description 198
238000001514 detection method Methods 0.000 title abstract description 18
238000000746 purification Methods 0.000 title abstract description 10
239000000758 substrate Substances 0.000 claims abstract description 129
239000000427 antigen Substances 0.000 claims abstract description 64
102000036639 antigens Human genes 0.000 claims abstract description 62
108091007433 antigens Proteins 0.000 claims abstract description 62
239000002052 molecular layer Substances 0.000 claims abstract description 11
229910052751 metal Inorganic materials 0.000 claims description 59
239000002184 metal Substances 0.000 claims description 59
229910052737 gold Inorganic materials 0.000 claims description 23
239000010931 gold Substances 0.000 claims description 23
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 22
239000000463 material Substances 0.000 claims description 15
229910052738 indium Inorganic materials 0.000 claims description 12
APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 claims description 12
239000011521 glass Substances 0.000 claims description 10
238000005259 measurement Methods 0.000 claims description 8
238000002405 diagnostic procedure Methods 0.000 claims description 7
238000002310 reflectometry Methods 0.000 claims description 6
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
238000000576 coating method Methods 0.000 claims description 5
238000001704 evaporation Methods 0.000 claims description 5
239000011248 coating agent Substances 0.000 claims description 4
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 3
239000011133 lead Substances 0.000 claims description 3
229910044991 metal oxide Inorganic materials 0.000 claims description 3
150000004706 metal oxides Chemical class 0.000 claims description 3
239000010445 mica Substances 0.000 claims description 3
229910052618 mica group Inorganic materials 0.000 claims description 3
239000004033 plastic Substances 0.000 claims description 3
239000010453 quartz Substances 0.000 claims description 3
229910052709 silver Inorganic materials 0.000 claims description 3
239000004332 silver Substances 0.000 claims description 3
239000011135 tin Substances 0.000 claims description 3
229910052718 tin Inorganic materials 0.000 claims description 3
239000005350 fused silica glass Substances 0.000 claims description 2
238000009736 wetting Methods 0.000 claims description 2
238000000151 deposition Methods 0.000 claims 5
RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims 2
239000012736 aqueous medium Substances 0.000 claims 2
239000010936 titanium Substances 0.000 claims 2
229910052719 titanium Inorganic materials 0.000 claims 2
239000012472 biological sample Substances 0.000 claims 1
239000000523 sample Substances 0.000 claims 1
239000002253 acid Substances 0.000 abstract description 25
230000001900 immune effect Effects 0.000 abstract description 10
230000003287 optical effect Effects 0.000 abstract description 4
239000000126 substance Substances 0.000 abstract description 3
230000000694 effects Effects 0.000 abstract 1
239000000243 solution Substances 0.000 description 76
238000012360 testing method Methods 0.000 description 40
208000006454 hepatitis Diseases 0.000 description 27
231100000283 hepatitis Toxicity 0.000 description 27
210000002966 serum Anatomy 0.000 description 22
239000010408 film Substances 0.000 description 16
108091003079 Bovine Serum Albumin Proteins 0.000 description 14
229940098773 bovine serum albumin Drugs 0.000 description 14
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 14
229910052753 mercury Inorganic materials 0.000 description 14
210000004369 blood Anatomy 0.000 description 13
239000008280 blood Substances 0.000 description 13
230000008105 immune reaction Effects 0.000 description 11
238000006243 chemical reaction Methods 0.000 description 9
239000002245 particle Substances 0.000 description 7
230000035945 sensitivity Effects 0.000 description 7
241001465754 Metazoa Species 0.000 description 6
108010058846 Ovalbumin Proteins 0.000 description 6
239000004793 Polystyrene Substances 0.000 description 6
230000008859 change Effects 0.000 description 6
230000000536 complexating effect Effects 0.000 description 6
230000005764 inhibitory process Effects 0.000 description 6
239000000203 mixture Substances 0.000 description 6
229940092253 ovalbumin Drugs 0.000 description 6
229920002223 polystyrene Polymers 0.000 description 6
210000002700 urine Anatomy 0.000 description 6
229910000497 Amalgam Inorganic materials 0.000 description 5
239000007788 liquid Substances 0.000 description 5
230000004048 modification Effects 0.000 description 5
238000012986 modification Methods 0.000 description 5
230000008569 process Effects 0.000 description 5
241000283973 Oryctolagus cuniculus Species 0.000 description 4
230000004520 agglutination Effects 0.000 description 4
239000003990 capacitor Substances 0.000 description 4
239000000470 constituent Substances 0.000 description 4
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
230000000875 corresponding effect Effects 0.000 description 3
238000001465 metallisation Methods 0.000 description 3
239000002244 precipitate Substances 0.000 description 3
230000008901 benefit Effects 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
238000003745 diagnosis Methods 0.000 description 2
238000009792 diffusion process Methods 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
201000010099 disease Diseases 0.000 description 2
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
230000008020 evaporation Effects 0.000 description 2
-1 fused sili~a Substances 0.000 description 2
238000007654 immersion Methods 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
238000009597 pregnancy test Methods 0.000 description 2
239000000047 product Substances 0.000 description 2
238000012216 screening Methods 0.000 description 2
241000894007 species Species 0.000 description 2
230000003595 spectral effect Effects 0.000 description 2
230000000007 visual effect Effects 0.000 description 2
241000518994 Conta Species 0.000 description 1
101100270435 Mus musculus Arhgef12 gene Proteins 0.000 description 1
241001163743 Perlodes Species 0.000 description 1
XDXHAEQXIBQUEZ-UHFFFAOYSA-N Ropinirole hydrochloride Chemical compound Cl.CCCN(CCC)CCC1=CC=CC2=C1CC(=O)N2 XDXHAEQXIBQUEZ-UHFFFAOYSA-N 0.000 description 1
241000950638 Symphysodon discus Species 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
230000009471 action Effects 0.000 description 1
230000004523 agglutinating effect Effects 0.000 description 1
238000013019 agitation Methods 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000009582 blood typing Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
JJCFRYNCJDLXIK-UHFFFAOYSA-N cyproheptadine Chemical compound C1CN(C)CCC1=C1C2=CC=CC=C2C=CC2=CC=CC=C21 JJCFRYNCJDLXIK-UHFFFAOYSA-N 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
238000010586 diagram Methods 0.000 description 1
230000005670 electromagnetic radiation Effects 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000009713 electroplating Methods 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
239000007888 film coating Substances 0.000 description 1
238000009501 film coating Methods 0.000 description 1
239000012212 insulator Substances 0.000 description 1
230000009545 invasion Effects 0.000 description 1
HOQADATXFBOEGG-UHFFFAOYSA-N isofenphos Chemical compound CCOP(=S)(NC(C)C)OC1=CC=CC=C1C(=O)OC(C)C HOQADATXFBOEGG-UHFFFAOYSA-N 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
230000000737 periodic effect Effects 0.000 description 1
230000000144 pharmacologic effect Effects 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
230000035935 pregnancy Effects 0.000 description 1
239000012460 protein solution Substances 0.000 description 1
230000004044 response Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
230000004936 stimulating effect Effects 0.000 description 1
229910052715 tantalum Inorganic materials 0.000 description 1
GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 description 1
238000010998 test method Methods 0.000 description 1
239000010409 thin film Substances 0.000 description 1
238000001429 visible spectrum Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated

Landscapes

Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Nanotechnology (AREA)
Molecular Biology (AREA)
Immunology (AREA)
General Health & Medical Sciences (AREA)
Crystallography & Structural Chemistry (AREA)
Urology & Nephrology (AREA)
Biotechnology (AREA)
Hematology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
General Physics & Mathematics (AREA)
Physics & Mathematics (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Composite Materials (AREA)
Materials Engineering (AREA)
Inorganic Chemistry (AREA)
Pharmacology & Pharmacy (AREA)
Medical Informatics (AREA)
Cell Biology (AREA)
General Engineering & Computer Science (AREA)
Condensed Matter Physics & Semiconductors (AREA)
Biophysics (AREA)
Food Science & Technology (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Investigating Or Analysing Materials By Optical Means (AREA)
Light Receiving Elements (AREA)
Peptides Or Proteins (AREA)

CA204,262A 1973-07-30 1974-07-08 Methode et appareil pour le depistage et la purification des proteines et des anticorps Expired CA1047918A (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US38411373A	1973-07-30	1973-07-30

Publications (1)

Publication Number	Publication Date
CA1047918A true CA1047918A (fr)	1979-02-06

Family

ID=23516091

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA204,262A Expired CA1047918A (fr)	1973-07-30	1974-07-08	Methode et appareil pour le depistage et la purification des proteines et des anticorps

Country Status (7)

Country	Link
JP (2)	JPS5944583B2 (fr)
BR (1)	BR7406086D0 (fr)
CA (1)	CA1047918A (fr)
DE (2)	DE2433246C2 (fr)
IT (1)	IT1212274B (fr)
NL (1)	NL177939C (fr)
SE (1)	SE464784B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5783455A (en) *	1994-10-15	1998-07-21	Behring Diagnostics Gmbh	Regenerable solid phase for carrying out specific binding reactions

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5238789A (en) *	1975-08-14	1977-03-25	Sinai School Medicine	Method of identifying hematocyte type and adaptability
DE2638250C2 (de) *	1975-08-27	1985-11-28	General Electric Co., Schenectady, N.Y.	Diagnostisches Verfahren zum Bestimmen der Anwesenheit oder Abwesenheit eines spezifischen Proteins in einer biologischen Probe, sowie Vorrichtung zur Durchführung des Verfahrens
DE2638251A1 (de) *	1975-08-27	1977-06-08	Gen Electric	Verfahren und vorrichtung zum konzentrieren und reinigen von antigenen und antikoerpern
EP0067921B1 (fr) *	1981-06-22	1987-11-11	Prutec Limited	Méthode pour déterminer des substances bioactives
DE3215484A1 (de) *	1982-04-26	1983-11-03	Sagax Instrument AB, 18302 Täby	Aus mehreren schichten bestehender schichtkoerper und verfahren zum nachweis und/oder messen der konzentration einer chemischen substanz, insbesondere biologischer herkunft
JPS6432150A (en) *	1987-07-29	1989-02-02	Teijin Ltd	Reflection color measuring instrument
JPH073388B2 (ja) *	1987-08-10	1995-01-18	帝人株式会社	膜厚変化測定装置
JPH0188778U (fr) *	1987-12-02	1989-06-12

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1025772A (fr) *	1972-06-26	1978-02-07	Ivar Giaever	Methode et appareil de detection et d'epuration des proteines et des anticorps

1974
- 1974-07-08 CA CA204,262A patent/CA1047918A/fr not_active Expired
- 1974-07-11 DE DE19742433246 patent/DE2433246C2/de not_active Expired
- 1974-07-11 DE DE19742463435 patent/DE2463435C2/de not_active Expired
- 1974-07-24 BR BR608674A patent/BR7406086D0/pt unknown
- 1974-07-29 SE SE7409777A patent/SE464784B/xx not_active IP Right Cessation
- 1974-07-29 IT IT2566674A patent/IT1212274B/it active
- 1974-07-30 NL NL7410231A patent/NL177939C/xx not_active IP Right Cessation
- 1974-07-30 JP JP8665874A patent/JPS5944583B2/ja not_active Expired
1983
- 1983-10-28 JP JP20117483A patent/JPS59160763A/ja active Granted

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5783455A (en) *	1994-10-15	1998-07-21	Behring Diagnostics Gmbh	Regenerable solid phase for carrying out specific binding reactions

Also Published As

Publication number	Publication date
DE2433246A1 (de)	1975-02-13
NL177939C (nl)	1985-12-16
JPS6130215B2 (fr)	1986-07-11
JPS5944583B2 (ja)	1984-10-30
DE2433246C2 (de)	1986-04-17
SE464784B (sv)	1991-06-10
JPS5076226A (fr)	1975-06-21
SE7409777L (fr)	1975-01-31
IT1212274B (it)	1989-11-22
BR7406086D0 (pt)	1975-05-13
DE2463435C2 (de)	1986-12-04
NL7410231A (nl)	1975-02-03
JPS59160763A (ja)	1984-09-11
NL177939B (nl)	1985-07-16

Publication	Publication Date	Title
US4054646A (en)	1977-10-18	Method and apparatus for detection of antibodies and antigens
CA1039632A (fr)	1978-10-03	Substrat pour epreuves immunologiques et methode de fabrication
CA1039649A (fr)	1978-10-03	Methode pour former des films d'un complexe immunologique en plusieurs couches
US3979509A (en)	1976-09-07	Opaque layer method for detecting biological particles
US3979184A (en)	1976-09-07	Diagnostic device for visually detecting presence of biological particles
JPS5938543B2 (ja)	1984-09-18	蚤白質および抗体の検出と精製のための方法並びに装置
DE68921635T2 (de)	1995-10-05	Kennzeichnung von makromolekülen in interaktion mit mindestens zwei liganden auf einem fühler.
EP0073980B1 (fr)	1985-12-27	Procédé et dispositif pour déterminer des composants biologiques
US3960490A (en)	1976-06-01	Method and apparatus for detecting immunologic reactions by diffusion in gel
DE68925426T2 (de)	1996-08-22	Oberflächen-plasmon-resonanz-sensor-einheit und deren verwendung in biosensor-systemen
Silin et al.	1997	SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs)
US5124172A (en)	1992-06-23	Thin film diagnostic device
CA1049400A (fr)	1979-02-27	Methode et appareil pour le dosage des particules biologiques ayant des proprietes immunologiques
US5716854A (en)	1998-02-10	Solid phase binding assay
JP2001503862A (ja)	2001-03-21	マス輸送補助光学アッセイのための方法及び装置
CA1047918A (fr)	1979-02-06	Methode et appareil pour le depistage et la purification des proteines et des anticorps
US7037727B1 (en)	2006-05-02	Apparatus for measuring a medical substance; a sensor for use in the apparatus; and a sensing element for use in the sensor
EP0500611A1 (fr)	1992-09-02	Dispositif et methodes d'analyse.
JP2000506610A (ja)	2000-05-30	免疫測定装置
JPS5916669B2 (ja)	1984-04-17	抗原又は抗体の検出方法
EP0287731A2 (fr)	1988-10-26	Eléments d'analyse secs avec une couche pour exclure les érythrocytes
CA1255585A (fr)	1989-06-13	Ligand marque a l'aide d'un donneur d'electrons ou d'un accepteur d'electrons
DE2638250A1 (de)	1977-03-10	Verbessertes verfahren zum nachweisen von antikoerpern und antigenen, sowie vorrichtung zur durchfuehrung des verfahrens
GB1562804A (en)	1980-03-19	Detection of antibodies and antigens
GB1564333A (en)	1980-04-10	Method for contrast in surface immunological tests

CA1047918A - Methode et appareil pour le depistage et la purification des proteines et des anticorps - Google Patents

Info

Links

Classifications

Landscapes

Applications Claiming Priority (1)

Publications (1)

Family

ID=23516091

Family Applications (1)

Country Status (7)

Cited By (1)

Families Citing this family (8)

Family Cites Families (1)

Cited By (1)

Also Published As

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