CA1070612A - Methode d'immunofluorescence en phase solide - Google Patents
Methode d'immunofluorescence en phase solideInfo
- Publication number
- CA1070612A CA1070612A CA255,623A CA255623A CA1070612A CA 1070612 A CA1070612 A CA 1070612A CA 255623 A CA255623 A CA 255623A CA 1070612 A CA1070612 A CA 1070612A
- Authority
- CA
- Canada
- Prior art keywords
- unknown
- particles
- antigen
- antibody
- immune reactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000003556 assay Methods 0.000 title claims abstract description 46
- 239000007790 solid phase Substances 0.000 title abstract description 4
- 239000000427 antigen Substances 0.000 claims abstract description 91
- 102000036639 antigens Human genes 0.000 claims abstract description 89
- 108091007433 antigens Proteins 0.000 claims abstract description 89
- 239000002245 particle Substances 0.000 claims abstract description 83
- 239000000376 reactant Substances 0.000 claims abstract description 74
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910001868 water Inorganic materials 0.000 claims abstract description 9
- 238000005259 measurement Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 3
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 11
- 230000027455 binding Effects 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 9
- 230000001900 immune effect Effects 0.000 claims description 8
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims description 7
- 229920002401 polyacrylamide Polymers 0.000 claims description 7
- 239000007900 aqueous suspension Substances 0.000 claims description 6
- 239000007795 chemical reaction product Substances 0.000 claims 1
- 238000010166 immunofluorescence Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 30
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 abstract description 8
- 229920001184 polypeptide Polymers 0.000 abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 101710100170 Unknown protein Proteins 0.000 abstract 1
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- 239000011324 bead Substances 0.000 description 63
- 239000000523 sample Substances 0.000 description 13
- 210000002966 serum Anatomy 0.000 description 12
- 239000006228 supernatant Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 241000283973 Oryctolagus cuniculus Species 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 5
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000000386 microscopy Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- 102000006395 Globulins Human genes 0.000 description 3
- 108010044091 Globulins Proteins 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 241000283707 Capra Species 0.000 description 2
- XPDXVDYUQZHFPV-UHFFFAOYSA-N Dansyl Chloride Chemical compound C1=CC=C2C(N(C)C)=CC=CC2=C1S(Cl)(=O)=O XPDXVDYUQZHFPV-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012875 competitive assay Methods 0.000 description 2
- 230000009137 competitive binding Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000005558 fluorometry Methods 0.000 description 2
- 229960004198 guanidine Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- 208000006454 hepatitis Diseases 0.000 description 2
- 231100000283 hepatitis Toxicity 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000700 radioactive tracer Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 229920003176 water-insoluble polymer Polymers 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
- IGHBXJSNZCFXNK-UHFFFAOYSA-N 4-chloro-7-nitrobenzofurazan Chemical compound [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12 IGHBXJSNZCFXNK-UHFFFAOYSA-N 0.000 description 1
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 241000489861 Maximus Species 0.000 description 1
- 241000609499 Palicourea Species 0.000 description 1
- 101710151715 Protein 7 Proteins 0.000 description 1
- -1 Rhodamine B isothiocyanate Chemical class 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 101710123661 Venom allergen 5 Proteins 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002532 anti-gammaglobulin Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 150000007942 carboxylates Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000000093 cytochemical effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000011325 microbead Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 150000003141 primary amines Chemical group 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229940043267 rhodamine b Drugs 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229920001897 terpolymer Polymers 0.000 description 1
- 229940034208 thyroxine Drugs 0.000 description 1
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US62119775A | 1975-10-09 | 1975-10-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1070612A true CA1070612A (fr) | 1980-01-29 |
Family
ID=24489155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA255,623A Expired CA1070612A (fr) | 1975-10-09 | 1976-06-24 | Methode d'immunofluorescence en phase solide |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS6010578B2 (fr) |
| BR (1) | BR7605664A (fr) |
| CA (1) | CA1070612A (fr) |
| DE (1) | DE2641713A1 (fr) |
| FR (1) | FR2327546A1 (fr) |
| GB (1) | GB1552374A (fr) |
| IT (1) | IT1066186B (fr) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1111762A (fr) * | 1977-12-28 | 1981-11-03 | David S. Frank | Chelate de terre rare fluorescent dans des particules de latex polymerique |
| US4171311A (en) * | 1978-01-16 | 1979-10-16 | International Diagnostics Tech. Inc. | Imides of thyroxin and triiodothyronine |
| EP0030087A1 (fr) * | 1979-11-13 | 1981-06-10 | Technicon Instruments Company Limited | Procédé et dispositif d'immuno-essais et trousse pour mise en oeuvre de ce procédé |
| DE3132491A1 (de) * | 1980-08-22 | 1982-07-01 | Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad | Verfahren zur durchfuehrung analytischer bestimmungen mittels der chemilumineszenzmethode und anwendung des verfahrens fuer immunassays |
| IT1140209B (it) * | 1981-09-25 | 1986-09-24 | Anic Spa | Reagenti per immunoluorescenza e metodo per la loro preparazione |
| JPS59108111U (ja) * | 1983-01-10 | 1984-07-20 | ダイキン工業株式会社 | 貯湯式給湯機 |
| JPS59220271A (ja) * | 1983-05-27 | 1984-12-11 | Kawasaki Heavy Ind Ltd | 複合材の鋳造法 |
| GB8401368D0 (en) * | 1984-01-19 | 1984-02-22 | Amersham Int Plc | Assay method |
| CN111007241A (zh) * | 2019-12-20 | 2020-04-14 | 苏州和迈精密仪器有限公司 | 基于双层多孔膜的荧光竞争法免疫检测方法、装置及应用 |
-
1976
- 1976-06-24 CA CA255,623A patent/CA1070612A/fr not_active Expired
- 1976-07-06 GB GB2810576A patent/GB1552374A/en not_active Expired
- 1976-07-23 FR FR7622676A patent/FR2327546A1/fr active Granted
- 1976-08-27 BR BR7605664A patent/BR7605664A/pt unknown
- 1976-09-10 JP JP10868176A patent/JPS6010578B2/ja not_active Expired
- 1976-09-16 DE DE19762641713 patent/DE2641713A1/de not_active Withdrawn
- 1976-09-17 IT IT5133076A patent/IT1066186B/it active
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6010578B2 (ja) | 1985-03-18 |
| JPS5246884A (en) | 1977-04-14 |
| GB1552374A (en) | 1979-09-12 |
| BR7605664A (pt) | 1977-08-23 |
| FR2327546A1 (fr) | 1977-05-06 |
| FR2327546B3 (fr) | 1979-04-13 |
| DE2641713A1 (de) | 1977-04-21 |
| IT1066186B (it) | 1985-03-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |