JPS6010578B2 - 固相免疫ケイ光分析法 - Google Patents

固相免疫ケイ光分析法

Info

Publication number: JPS6010578B2
Authority: JP; Japan
Prior art keywords: antigen; antibody; beads; immune system; particles
Prior art date: 1975-10-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP10868176A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5246884A (en

Inventor

ジエームス・エフ・モンソニー

マイケル・ダブリユー・バーゲツト

ロバート・ブイ・ダールストロム

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bio Rad Laboratories Inc

Original Assignee

Bio Rad Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1975-10-09

Filing date

1976-09-10

Publication date

1985-03-18

1976-09-10 Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc

1977-04-14 Publication of JPS5246884A publication Critical patent/JPS5246884A/ja

1985-03-18 Publication of JPS6010578B2 publication Critical patent/JPS6010578B2/ja

Status Expired legal-status Critical Current

Links

238000010185 immunofluorescence analysis Methods 0.000 title claims description 3
239000007790 solid phase Substances 0.000 title 1
239000002245 particle Substances 0.000 claims description 33
210000000987 immune system Anatomy 0.000 claims description 24
239000000376 reactant Substances 0.000 claims description 23
238000004458 analytical method Methods 0.000 claims description 10
239000007864 aqueous solution Substances 0.000 claims description 5
230000027455 binding Effects 0.000 claims description 5
229920001477 hydrophilic polymer Polymers 0.000 claims description 2
239000007900 aqueous suspension Substances 0.000 claims 1
239000000427 antigen Substances 0.000 description 73
102000036639 antigens Human genes 0.000 description 72
108091007433 antigens Proteins 0.000 description 72
239000011324 bead Substances 0.000 description 59
238000000034 method Methods 0.000 description 28
239000000523 sample Substances 0.000 description 11
210000002966 serum Anatomy 0.000 description 11
238000012360 testing method Methods 0.000 description 11
238000006243 chemical reaction Methods 0.000 description 10
239000008363 phosphate buffer Substances 0.000 description 9
102000004169 proteins and genes Human genes 0.000 description 8
108090000623 proteins and genes Proteins 0.000 description 8
239000006228 supernatant Substances 0.000 description 8
239000000725 suspension Substances 0.000 description 8
241000283973 Oryctolagus cuniculus Species 0.000 description 7
229920002401 polyacrylamide Polymers 0.000 description 7
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
238000005119 centrifugation Methods 0.000 description 5
230000001900 immune effect Effects 0.000 description 5
239000000243 solution Substances 0.000 description 5
LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 4
102000006395 Globulins Human genes 0.000 description 4
108010044091 Globulins Proteins 0.000 description 4
ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 4
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
210000004027 cell Anatomy 0.000 description 4
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 4
239000000203 mixture Substances 0.000 description 4
229920000642 polymer Polymers 0.000 description 4
230000009137 competitive binding Effects 0.000 description 3
238000001514 detection method Methods 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
229910052698 phosphorus Inorganic materials 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
BLWINLJDTOJSRU-UHFFFAOYSA-N 2-methoxy-2,4-diphenylfuran-3-one Chemical compound O=C1C(OC)(C=2C=CC=CC=2)OC=C1C1=CC=CC=C1 BLWINLJDTOJSRU-UHFFFAOYSA-N 0.000 description 2
IGHBXJSNZCFXNK-UHFFFAOYSA-N 4-chloro-7-nitrobenzofurazan Chemical compound [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12 IGHBXJSNZCFXNK-UHFFFAOYSA-N 0.000 description 2
FWEOQOXTVHGIFQ-UHFFFAOYSA-N 8-anilinonaphthalene-1-sulfonic acid Chemical compound C=12C(S(=O)(=O)O)=CC=CC2=CC=CC=1NC1=CC=CC=C1 FWEOQOXTVHGIFQ-UHFFFAOYSA-N 0.000 description 2
229920000936 Agarose Polymers 0.000 description 2
241000283707 Capra Species 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 2
229930182555 Penicillin Natural products 0.000 description 2
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
239000002253 acid Substances 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
239000013068 control sample Substances 0.000 description 2
238000012258 culturing Methods 0.000 description 2
238000010908 decantation Methods 0.000 description 2
SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 2
230000005284 excitation Effects 0.000 description 2
ZFKJVJIDPQDDFY-UHFFFAOYSA-N fluorescamine Chemical compound C12=CC=CC=C2C(=O)OC1(C1=O)OC=C1C1=CC=CC=C1 ZFKJVJIDPQDDFY-UHFFFAOYSA-N 0.000 description 2
208000006454 hepatitis Diseases 0.000 description 2
231100000283 hepatitis Toxicity 0.000 description 2
239000007788 liquid Substances 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
238000005259 measurement Methods 0.000 description 2
239000008188 pellet Substances 0.000 description 2
229940049954 penicillin Drugs 0.000 description 2
229920001184 polypeptide Polymers 0.000 description 2
239000000843 powder Substances 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
102000004196 processed proteins & peptides Human genes 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
239000011541 reaction mixture Substances 0.000 description 2
238000012800 visualization Methods 0.000 description 2
UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
XPDXVDYUQZHFPV-UHFFFAOYSA-N Dansyl Chloride Chemical compound C1=CC=C2C(N(C)C)=CC=CC2=C1S(Cl)(=O)=O XPDXVDYUQZHFPV-UHFFFAOYSA-N 0.000 description 1
102000008100 Human Serum Albumin Human genes 0.000 description 1
108091006905 Human Serum Albumin Proteins 0.000 description 1
241000609499 Palicourea Species 0.000 description 1
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
-1 Rhodamine B isothiocyanate Chemical class 0.000 description 1
230000004520 agglutination Effects 0.000 description 1
238000003556 assay Methods 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
238000009835 boiling Methods 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000007975 buffered saline Substances 0.000 description 1
150000007942 carboxylates Chemical class 0.000 description 1
238000007385 chemical modification Methods 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
238000012875 competitive assay Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
239000008367 deionised water Substances 0.000 description 1
229910021641 deionized water Inorganic materials 0.000 description 1
238000010586 diagram Methods 0.000 description 1
238000009826 distribution Methods 0.000 description 1
230000000694 effects Effects 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
HBNYJWAFDZLWRS-UHFFFAOYSA-N ethyl isothiocyanate Chemical compound CCN=C=S HBNYJWAFDZLWRS-UHFFFAOYSA-N 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000011049 filling Methods 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
238000001215 fluorescent labelling Methods 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
229940127121 immunoconjugate Drugs 0.000 description 1
JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000004816 latex Substances 0.000 description 1
229920000126 latex Polymers 0.000 description 1
239000010985 leather Substances 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
238000000399 optical microscopy Methods 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
239000011574 phosphorus Substances 0.000 description 1
239000011148 porous material Substances 0.000 description 1
239000012460 protein solution Substances 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
239000000700 radioactive tracer Substances 0.000 description 1
229920013730 reactive polymer Polymers 0.000 description 1
229940043267 rhodamine b Drugs 0.000 description 1
230000028327 secretion Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
241000894007 species Species 0.000 description 1
238000004611 spectroscopical analysis Methods 0.000 description 1
150000003431 steroids Chemical class 0.000 description 1
239000000758 substrate Substances 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
229940034208 thyroxine Drugs 0.000 description 1
XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
238000005406 washing Methods 0.000 description 1
229920003176 water-insoluble polymer Polymers 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

Health & Medical Sciences (AREA)
Immunology (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

JP10868176A 1975-10-09 1976-09-10 固相免疫ケイ光分析法 Expired JPS6010578B2 (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US62119775A	1975-10-09	1975-10-09
US621197		1990-11-30

Publications (2)

Publication Number	Publication Date
JPS5246884A JPS5246884A (en)	1977-04-14
JPS6010578B2 true JPS6010578B2 (ja)	1985-03-18

Family

ID=24489155

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP10868176A Expired JPS6010578B2 (ja)	1975-10-09	1976-09-10	固相免疫ケイ光分析法

Country Status (7)

Country	Link
JP (1)	JPS6010578B2 (fr)
BR (1)	BR7605664A (fr)
CA (1)	CA1070612A (fr)
DE (1)	DE2641713A1 (fr)
FR (1)	FR2327546A1 (fr)
GB (1)	GB1552374A (fr)
IT (1)	IT1066186B (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1111762A (fr) *	1977-12-28	1981-11-03	David S. Frank	Chelate de terre rare fluorescent dans des particules de latex polymerique
US4171311A (en) *	1978-01-16	1979-10-16	International Diagnostics Tech. Inc.	Imides of thyroxin and triiodothyronine
EP0030087A1 (fr) *	1979-11-13	1981-06-10	Technicon Instruments Company Limited	Procédé et dispositif d'immuno-essais et trousse pour mise en oeuvre de ce procédé
DE3132491A1 (de) *	1980-08-22	1982-07-01	Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad	Verfahren zur durchfuehrung analytischer bestimmungen mittels der chemilumineszenzmethode und anwendung des verfahrens fuer immunassays
IT1140209B (it) *	1981-09-25	1986-09-24	Anic Spa	Reagenti per immunoluorescenza e metodo per la loro preparazione
JPS59108111U (ja) *	1983-01-10	1984-07-20	ダイキン工業株式会社	貯湯式給湯機
JPS59220271A (ja) *	1983-05-27	1984-12-11	Kawasaki Heavy Ind Ltd	複合材の鋳造法
GB8401368D0 (en) *	1984-01-19	1984-02-22	Amersham Int Plc	Assay method
CN111007241A (zh) *	2019-12-20	2020-04-14	苏州和迈精密仪器有限公司	基于双层多孔膜的荧光竞争法免疫检测方法、装置及应用

1976
- 1976-06-24 CA CA255,623A patent/CA1070612A/fr not_active Expired
- 1976-07-06 GB GB2810576A patent/GB1552374A/en not_active Expired
- 1976-07-23 FR FR7622676A patent/FR2327546A1/fr active Granted
- 1976-08-27 BR BR7605664A patent/BR7605664A/pt unknown
- 1976-09-10 JP JP10868176A patent/JPS6010578B2/ja not_active Expired
- 1976-09-16 DE DE19762641713 patent/DE2641713A1/de not_active Withdrawn
- 1976-09-17 IT IT5133076A patent/IT1066186B/it active

Also Published As

Publication number	Publication date
JPS5246884A (en)	1977-04-14
GB1552374A (en)	1979-09-12
BR7605664A (pt)	1977-08-23
FR2327546A1 (fr)	1977-05-06
CA1070612A (fr)	1980-01-29
FR2327546B3 (fr)	1979-04-13
DE2641713A1 (de)	1977-04-21
IT1066186B (it)	1985-03-04

Publication	Publication Date	Title
US4254096A (en)	1981-03-03	Reagent combination for solid phase immunofluorescent assay
US4201763A (en)	1980-05-06	Solid phase immunofluorescent assay method
US5413913A (en)	1995-05-09	Erythrocyte agglutination assay
US4433059A (en)	1984-02-21	Double antibody conjugate
JPH09506172A (ja)	1997-06-17	汎用的な抗ハプテン抗体を使用するイムノアッセイ及び該イムノアッセイで使用される材料
US4894347A (en)	1990-01-16	Erythrocyte agglutination assay
JPH06258325A (ja)	1994-09-16	異なる２又は３種類のモノクローナル抗体を使用する抗原測定法及び該モノクローナル抗体を利用した試験キット
AU654833B2 (en)	1994-11-24	The use of pairs of peptides with extremely high specific affinity for one another in the area of in vitro diagnosis
AU3939493A (en)	1993-11-08	Immunoassays employing generic anti-hapten antibodies and materials for use therein
WO1992021974A1 (fr)	1992-12-10	Compositions stables a base de phosphatase alcaline a coloration amelioree, et leur utilisation dans des dosages
US6500629B1 (en)	2002-12-31	Materials and methods for detection and quantitation of an analyte
JPS6010578B2 (ja)	1985-03-18	固相免疫ケイ光分析法
US4623618A (en)	1986-11-18	Simultaneous quantitative immunoassay for different antigens or antibodies
JP2007510165A (ja)	2007-04-19	結合アッセイ成分
JPS6247555A (ja)	1987-03-02	シンチレ−シヨン近接定量法
EP0308242B1 (fr)	1995-01-04	Essai d'agglutination
JPH05502098A (ja)	1993-04-15	凝集検定
JPH0611507A (ja)	1994-01-21	ヒトポドカリキシンの測定方法
JP2596959B2 (ja)	1997-04-02	リガンドのアッセイ法
JP2931111B2 (ja)	1999-08-09	癌診断剤
JPS5960260A (ja)	1984-04-06	酵素免疫測定法
JPS6012576B2 (ja)	1985-04-02	サイモポイエチンの免疫検定
DE69332454T2 (de)	2003-03-20	Trennverfahren
AP81A (en)	1990-04-28	Agglutination assay
JPS63231265A (ja)	1988-09-27	凝集反応試薬及びその製造方法