CA1212320A - Ozone inactivated organisms as vaccines - Google Patents

Ozone inactivated organisms as vaccines

Info

Publication number: CA1212320A
Authority: CA; Canada
Prior art keywords: ozone; microorganism; inactivated; virus; vaccine
Prior art date: 1982-02-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000420401A

Other languages

English (en)

French (fr)

Inventor

Yuan C. Zee

David C. Bolton

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of California San Diego UCSD

Original Assignee

University of California San Diego UCSD

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-02-09

Filing date

1983-01-27

Publication date

1986-10-07

1983-01-27 Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD

1986-10-07 Application granted granted Critical

1986-10-07 Publication of CA1212320A publication Critical patent/CA1212320A/en

Status Expired legal-status Critical Current

Links

CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 title claims abstract description 47
229960005486 vaccine Drugs 0.000 title claims abstract description 24
244000005700 microbiome Species 0.000 claims abstract description 36
230000009851 immunogenic response Effects 0.000 claims abstract description 4
241000700605 Viruses Species 0.000 claims description 29
238000000034 method Methods 0.000 claims description 9
241000712461 unidentified influenza virus Species 0.000 claims description 6
230000003053 immunization Effects 0.000 claims description 5
230000000415 inactivating effect Effects 0.000 claims description 4
244000000010 microbial pathogen Species 0.000 claims description 4
241000894006 Bacteria Species 0.000 claims description 3
238000004519 manufacturing process Methods 0.000 claims description 3
239000000126 substance Substances 0.000 claims description 2
238000002255 vaccination Methods 0.000 abstract description 4
241000699670 Mus sp. Species 0.000 description 22
230000002779 inactivation Effects 0.000 description 14
239000002609 medium Substances 0.000 description 12
239000007789 gas Substances 0.000 description 11
244000052769 pathogen Species 0.000 description 8
239000000725 suspension Substances 0.000 description 8
235000015097 nutrients Nutrition 0.000 description 7
230000003472 neutralizing effect Effects 0.000 description 6
230000001717 pathogenic effect Effects 0.000 description 6
238000012360 testing method Methods 0.000 description 6
239000000443 aerosol Substances 0.000 description 5
239000010409 thin film Substances 0.000 description 5
108091003079 Bovine Serum Albumin Proteins 0.000 description 4
241001465754 Metazoa Species 0.000 description 4
239000012894 fetal calf serum Substances 0.000 description 4
239000012530 fluid Substances 0.000 description 4
210000000987 immune system Anatomy 0.000 description 4
230000000937 inactivator Effects 0.000 description 4
210000004072 lung Anatomy 0.000 description 4
239000006145 Eagle's minimal essential medium Substances 0.000 description 3
239000000232 Lipid Bilayer Substances 0.000 description 3
241000124008 Mammalia Species 0.000 description 3
239000000427 antigen Substances 0.000 description 3
102000036639 antigens Human genes 0.000 description 3
108091007433 antigens Proteins 0.000 description 3
238000004113 cell culture Methods 0.000 description 3
239000006185 dispersion Substances 0.000 description 3
230000028993 immune response Effects 0.000 description 3
208000015181 infectious disease Diseases 0.000 description 3
238000002347 injection Methods 0.000 description 3
239000007924 injection Substances 0.000 description 3
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230000003612 virological effect Effects 0.000 description 3
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
206010001497 Agitation Diseases 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
241000233866 Fungi Species 0.000 description 2
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
229930182566 Gentamicin Natural products 0.000 description 2
239000007995 HEPES buffer Substances 0.000 description 2
241000712431 Influenza A virus Species 0.000 description 2
238000013019 agitation Methods 0.000 description 2
230000000890 antigenic effect Effects 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
239000005388 borosilicate glass Substances 0.000 description 2
230000001413 cellular effect Effects 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
201000010099 disease Diseases 0.000 description 2
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
229960002518 gentamicin Drugs 0.000 description 2
230000002163 immunogen Effects 0.000 description 2
238000010348 incorporation Methods 0.000 description 2
206010022000 influenza Diseases 0.000 description 2
239000000203 mixture Substances 0.000 description 2
229910052760 oxygen Inorganic materials 0.000 description 2
239000001301 oxygen Substances 0.000 description 2
230000001105 regulatory effect Effects 0.000 description 2
210000002966 serum Anatomy 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
208000024891 symptom Diseases 0.000 description 2
231100000331 toxic Toxicity 0.000 description 2
230000002588 toxic effect Effects 0.000 description 2
230000009385 viral infection Effects 0.000 description 2
PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 1
TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 1
IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
241000606125 Bacteroides Species 0.000 description 1
241000537222 Betabaculovirus Species 0.000 description 1
241000588807 Bordetella Species 0.000 description 1
241000701083 Bovine alphaherpesvirus 1 Species 0.000 description 1
241000606161 Chlamydia Species 0.000 description 1
241000711573 Coronaviridae Species 0.000 description 1
101100511454 Epichloe uncinata lolO1 gene Proteins 0.000 description 1
101100511455 Epichloe uncinata lolO2 gene Proteins 0.000 description 1
241000283073 Equus caballus Species 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
229920002449 FKM Polymers 0.000 description 1
JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 1
241000606790 Haemophilus Species 0.000 description 1
241000700586 Herpesviridae Species 0.000 description 1
206010020751 Hypersensitivity Diseases 0.000 description 1
229940124873 Influenza virus vaccine Drugs 0.000 description 1
241000186781 Listeria Species 0.000 description 1
241000204031 Mycoplasma Species 0.000 description 1
241000588653 Neisseria Species 0.000 description 1
241000712464 Orthomyxoviridae Species 0.000 description 1
241000711504 Paramyxoviridae Species 0.000 description 1
241000702247 Reoviridae Species 0.000 description 1
241000711931 Rhabdoviridae Species 0.000 description 1
241000607142 Salmonella Species 0.000 description 1
241000607768 Shigella Species 0.000 description 1
241000191940 Staphylococcus Species 0.000 description 1
241000194017 Streptococcus Species 0.000 description 1
239000004809 Teflon Substances 0.000 description 1
229920006362 Teflon® Polymers 0.000 description 1
241000710924 Togaviridae Species 0.000 description 1
241000607598 Vibrio Species 0.000 description 1
LEBBDRXHHNYZIA-LDUWYPJVSA-N [(2s,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] n-[(z)-1,3-dihydroxyoctadec-4-en-2-yl]carbamate Chemical compound CCCCCCCCCCCCC\C=C/C(O)C(CO)NC(=O)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O LEBBDRXHHNYZIA-LDUWYPJVSA-N 0.000 description 1
230000004913 activation Effects 0.000 description 1
150000007933 aliphatic carboxylic acids Chemical class 0.000 description 1
208000026935 allergic disease Diseases 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
229940126575 aminoglycoside Drugs 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
230000005875 antibody response Effects 0.000 description 1
238000013459 approach Methods 0.000 description 1
230000000721 bacterilogical effect Effects 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
239000012620 biological material Substances 0.000 description 1
239000000872 buffer Substances 0.000 description 1
125000004432 carbon atom Chemical group C* 0.000 description 1
235000012000 cholesterol Nutrition 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000001816 cooling Methods 0.000 description 1
230000006378 damage Effects 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
ZGSPNIOCEDOHGS-UHFFFAOYSA-L disodium [3-[2,3-di(octadeca-9,12-dienoyloxy)propoxy-oxidophosphoryl]oxy-2-hydroxypropyl] 2,3-di(octadeca-9,12-dienoyloxy)propyl phosphate Chemical compound [Na+].[Na+].CCCCCC=CCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COP([O-])(=O)OCC(O)COP([O-])(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COC(=O)CCCCCCCC=CCC=CCCCCC ZGSPNIOCEDOHGS-UHFFFAOYSA-L 0.000 description 1
230000000694 effects Effects 0.000 description 1
238000010891 electric arc Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
-1 etc. sphingomyelin Chemical compound 0.000 description 1
239000010408 film Substances 0.000 description 1
239000012467 final product Substances 0.000 description 1
238000009472 formulation Methods 0.000 description 1
238000007710 freezing Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
239000011521 glass Substances 0.000 description 1
244000144993 groups of animals Species 0.000 description 1
230000028996 humoral immune response Effects 0.000 description 1
230000009610 hypersensitivity Effects 0.000 description 1
230000005847 immunogenicity Effects 0.000 description 1
229940031551 inactivated vaccine Drugs 0.000 description 1
229960003971 influenza vaccine Drugs 0.000 description 1
230000009545 invasion Effects 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
229940067606 lecithin Drugs 0.000 description 1
235000010445 lecithin Nutrition 0.000 description 1
239000000787 lecithin Substances 0.000 description 1
239000012669 liquid formulation Substances 0.000 description 1
230000007774 longterm Effects 0.000 description 1
239000008176 lyophilized powder Substances 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
QZIQJVCYUQZDIR-UHFFFAOYSA-N mechlorethamine hydrochloride Chemical compound Cl.ClCCN(C)CCCl QZIQJVCYUQZDIR-UHFFFAOYSA-N 0.000 description 1
238000002941 microtiter virus yield reduction assay Methods 0.000 description 1
239000007758 minimum essential medium Substances 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
244000309711 non-enveloped viruses Species 0.000 description 1
239000002245 particle Substances 0.000 description 1
WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
150000003904 phospholipids Chemical class 0.000 description 1
239000000902 placebo Substances 0.000 description 1
229940068196 placebo Drugs 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000005057 refrigeration Methods 0.000 description 1
238000011160 research Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000000243 solution Substances 0.000 description 1
239000007921 spray Substances 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
238000007920 subcutaneous administration Methods 0.000 description 1
230000000153 supplemental effect Effects 0.000 description 1
230000002459 sustained effect Effects 0.000 description 1
239000003104 tissue culture media Substances 0.000 description 1
238000004448 titration Methods 0.000 description 1
210000002845 virion Anatomy 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5252—Virus inactivated (killed)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16161—Methods of inactivation or attenuation
- C12N2760/16163—Methods of inactivation or attenuation by chemical treatment

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Virology (AREA)
Microbiology (AREA)
Immunology (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Veterinary Medicine (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Engineering & Computer Science (AREA)
Epidemiology (AREA)
Mycology (AREA)
Pharmacology & Pharmacy (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Pulmonology (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

CA000420401A 1982-02-09 1983-01-27 Ozone inactivated organisms as vaccines Expired CA1212320A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US34856982A	1982-02-09	1982-02-09
US348,569		1982-02-09

Publications (1)

Publication Number	Publication Date
CA1212320A true CA1212320A (en)	1986-10-07

Family

ID=23368584

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000420401A Expired CA1212320A (en)	1982-02-09	1983-01-27	Ozone inactivated organisms as vaccines

Country Status (10)

Country	Link
EP (1)	EP0086071B1 (da)
JP (1)	JPS58201721A (da)
AT (1)	ATE16348T1 (da)
AU (1)	AU558008B2 (da)
CA (1)	CA1212320A (da)
DE (1)	DE3361138D1 (da)
DK (1)	DK52783A (da)
IL (1)	IL67818A (da)
NZ (1)	NZ203094A (da)
ZA (1)	ZA83640B (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3345864A1 (de) *	1983-12-19	1985-06-27	Egbert Frh. von 8000 München Malsen-Ponickau	Impfstoff und verfahren zu seiner herstellung
US4632980A (en) *	1985-04-03	1986-12-30	Immunologics	Ozone decontamination of blood and blood products
EP0227441B1 (en) *	1985-12-20	1991-05-15	E.I. Du Pont De Nemours And Company	Preparation of tablets of microbiological substances
FR2603805A1 (fr) *	1986-09-16	1988-03-18	Transfusion Sanguine Assoc Rgl	Procede d'inactivation par ozonolyse de micro-organismes contaminants presents dans des materiaux biologiques essentiellement proteiques, les produits obtenus et leurs applications biologiques et analytiques
GB0603749D0 (en) *	2006-02-24	2006-04-05	Moving Sun Ltd	Immune system stimulant and process of manufacturing the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
BE471250A (da) *
CH173194A (de) *	1933-05-27	1934-11-15	Veterinaria A G Zuerich	Verfahren zur Herstellung eines Impfstoffes zur aktiven Immunisierung von Menschen oder Tieren gegen anaerobe Krankheitskeime.
CH200180A (de) *	1936-03-26	1938-09-30	Frei Walter Dr Prof	Verfahren zur Herstellung von Vaccinen.
GB1230414A (da) *	1968-07-09	1971-05-05

1983
- 1983-01-26 NZ NZ203094A patent/NZ203094A/en unknown
- 1983-01-27 CA CA000420401A patent/CA1212320A/en not_active Expired
- 1983-01-27 AU AU10812/83A patent/AU558008B2/en not_active Expired - Fee Related
- 1983-01-31 ZA ZA83640A patent/ZA83640B/xx unknown
- 1983-01-31 EP EP83300483A patent/EP0086071B1/en not_active Expired
- 1983-01-31 AT AT83300483T patent/ATE16348T1/de not_active IP Right Cessation
- 1983-01-31 DE DE8383300483T patent/DE3361138D1/de not_active Expired
- 1983-02-01 IL IL67818A patent/IL67818A/xx unknown
- 1983-02-08 DK DK52783A patent/DK52783A/da not_active Application Discontinuation
- 1983-02-08 JP JP58018265A patent/JPS58201721A/ja active Pending

Also Published As

Publication number	Publication date
NZ203094A (en)	1986-04-11
DK52783D0 (da)	1983-02-08
EP0086071B1 (en)	1985-11-06
ATE16348T1 (de)	1985-11-15
DE3361138D1 (en)	1985-12-12
AU1081283A (en)	1983-08-18
JPS58201721A (ja)	1983-11-24
AU558008B2 (en)	1987-01-15
IL67818A (en)	1987-03-31
IL67818A0 (en)	1983-06-15
ZA83640B (en)	1984-03-28
DK52783A (da)	1983-08-10
EP0086071A1 (en)	1983-08-17

Legal Events

Date	Code	Title	Description
2003-10-07	MKEX	Expiry

Publication	Publication Date	Title
US4632980A (en)	1986-12-30	Ozone decontamination of blood and blood products
Rosendal et al.	1988	Evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of Haemophilus (Actinobacillus) pleuropneumoniae
JP4243349B2 (ja)	2009-03-25	インフルエンザウイルスの複製のための動物細胞および方法
Blake et al.	1981	Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae
Thacore et al.	1969	Cells persistently infected with newcastle disease virus: I. Properties of mutants isolated from persistently infected L cells
JP2008245659A (ja)	2008-10-16	細胞培養におけるインフルエンザウイルスの複製の方法、および本方法によって入手可能なインフルエンザウイルス
Pauli et al.	1985	Increase of virus yields and releases of Borna disease virus from persistently infected cells
CA1212320A (en)	1986-10-07	Ozone inactivated organisms as vaccines
AU2007305595B2 (en)	2012-10-04	IPV-DPT vaccine
Kohn et al.	1980	Interaction of polyunsaturated fatty acids with animal cells and enveloped viruses
Appleby	1952	The isolation and properties of a modified strain of neurotropic influenza A virus
Haff et al.	1965	Role of sialic acid receptors in adsorption of influenza virus to chick embryo cells
Hackemann et al.	1974	Inactivation of influenza virus by human lymphocytes
RU2142816C1 (ru)	1999-12-20	Способ получения антигерпетической вакцины и лекарственная форма на ее основе
Kasel et al.	1961	Further characterization of the adenovirus erythrocyte receptor-modifying factor
JP2889923B2 (ja)	1999-05-10	百日咳トキソイドワクチン
AU637374B2 (en)	1993-05-27	Ozone decontamination of blood and blood products
宮前武雄	1987	Mouse nasal immunogenicity of Sendai virus vaccines inactivated by chemical modification of core RNA.
HK1129836B (en)	2016-06-24	Ipv-dpt vaccine
MOORE	1904	A chemical theory as to the propagation and development of certain infectious diseases
JPH02257877A (ja)	1990-10-18	リンホカイン活性化キラー細胞の製造法