CA2060314A1 - Agarose a faible electro-osmose - Google Patents

Agarose a faible electro-osmose

Info

Publication number
CA2060314A1
CA2060314A1 CA2060314A CA2060314A CA2060314A1 CA 2060314 A1 CA2060314 A1 CA 2060314A1 CA 2060314 A CA2060314 A CA 2060314A CA 2060314 A CA2060314 A CA 2060314A CA 2060314 A1 CA2060314 A1 CA 2060314A1
Authority
CA
Canada
Prior art keywords
agarose
less
concentration
purified
content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2060314A
Other languages
English (en)
Other versions
CA2060314C (fr
Inventor
Francis H. Kirkpatrick
Kenneth Guiseley
Richard Provonchee
Samuel Nochumson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FMC Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2060314A1 publication Critical patent/CA2060314A1/fr
Application granted granted Critical
Publication of CA2060314C publication Critical patent/CA2060314C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0036—Galactans; Derivatives thereof
    • C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44752—Controlling the zeta potential, e.g. by wall coatings

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Environmental & Geological Engineering (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Colloid Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002060314A 1989-08-03 1990-07-31 Agarose a faible electro-osmose Expired - Fee Related CA2060314C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US389,141 1989-08-03
US07/389,141 US4983268A (en) 1989-08-03 1989-08-03 High gel strength low electroendosmosis agarose

Publications (2)

Publication Number Publication Date
CA2060314A1 true CA2060314A1 (fr) 1991-02-04
CA2060314C CA2060314C (fr) 1994-09-06

Family

ID=23537006

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002060314A Expired - Fee Related CA2060314C (fr) 1989-08-03 1990-07-31 Agarose a faible electro-osmose

Country Status (5)

Country Link
US (1) US4983268A (fr)
EP (1) EP0485510A4 (fr)
JP (1) JPH087187B2 (fr)
CA (1) CA2060314C (fr)
WO (1) WO1991001995A1 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0454763T3 (da) * 1989-01-13 1996-11-25 Fmc Corp Adskillelsesgeler og gelsystemer på polysaccharidbasis til stabelelektroforese og fremgangsmåde til støbning af en elektroforesegel
JPH0776242B2 (ja) * 1990-06-13 1995-08-16 エフ エム シー コーポレーション 誘導体化されたアガロース生成物およびその製法
DE69016014T2 (de) * 1990-07-04 1995-08-31 Marcin Krotkiewski Blutdrucksenkendes Präparat.
US5230832A (en) * 1991-01-24 1993-07-27 Brandeis University Galactomannan-agarose binary gel for nucleic acid electrophoresis
US5455344A (en) * 1993-09-03 1995-10-03 Fmc Corporation Agarose compositions for nucleic acid sequencing
FR2722295B1 (fr) * 1994-07-07 1996-10-04 Roussy Inst Gustave Methode d'analyse d'adn dite sscp et gel d'electro-phorene
US8071133B2 (en) * 2001-08-20 2011-12-06 Stiefel Laboratories, Inc. Oral dosage forms of water insoluble drugs and methods of making the same
US20040115266A1 (en) * 2002-02-01 2004-06-17 Namburi Ranga Raju Oral itraconazole formulations and methods of making the same
US20050267296A1 (en) * 2004-06-01 2005-12-01 Council Of Scientific & Industrial Research Cost-effective process for preparing agarose from gracilaria spp.
RU2417090C2 (ru) * 2005-09-26 2011-04-27 Дзе Рогозин Инститьют, Инк. Макрогранулы агарозы seakem gold, содержащие секреторные клетки, и их применение
EP2408482A1 (fr) 2009-03-19 2012-01-25 Millipore Corporation Élimination de micro-organismes dans des échantillons de fluide en utilisant des milieux de filtration à nanofibres
SG185659A1 (en) 2010-08-10 2012-12-28 Emd Millipore Corp Method for retrovirus removal
CN105413480B (zh) 2011-04-01 2019-03-29 Emd密理博公司 含有纳米纤维的复合材料结构
KR20190011838A (ko) 2014-06-26 2019-02-07 이엠디 밀리포어 코포레이션 개선된 먼지 포집 능력을 갖는 필터 구조
KR102206959B1 (ko) 2015-04-17 2021-01-25 이엠디 밀리포어 코포레이션 접선방향 유동 여과 모드에서 작동되는 나노섬유 한외여과막을 사용하여 샘플에서 목적하는 생물학적 물질을 정제하는 방법
EP3655142B1 (fr) 2017-07-21 2026-02-25 Merck Millipore Ltd Membranes de fibres non tissées
CN108835612A (zh) * 2018-06-20 2018-11-20 罗国球 一种高温低浓度碱制制取琼脂的方法
US11691122B2 (en) 2018-11-02 2023-07-04 Advanced Aesthetic Technologies, Inc. Irradiated agarose, compositions thereof, and related methods
CN112996551B (zh) * 2018-11-02 2023-08-29 先进美学科技公司 辐照琼脂糖、其组合物及相关方法
CN110698573B (zh) * 2019-11-19 2021-07-30 中国科学院海洋研究所 一种高品质琼脂糖的制备方法
CN115260341B (zh) * 2022-07-29 2023-05-02 国药集团化学试剂有限公司 一种高质量琼脂糖及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB639222A (en) * 1941-06-15 1950-06-21 Tjoa Sie Lian Process for purifying agar-agar
US3753972A (en) * 1970-04-28 1973-08-21 Research Corp Fractionation of agar
US4290911A (en) * 1979-02-07 1981-09-22 Fmc Corporation Agarose composition, aqueous gel and method of making same
CA1206666A (fr) * 1982-02-19 1986-06-24 Samuel Nochumson Copolymere d'acrylamide et de resine polysaccharidique utilise comme milieu pour l'electrophorese sur gel
US4990611A (en) * 1987-08-17 1991-02-05 Fmc Corporation Agarose purification method using glycol

Also Published As

Publication number Publication date
EP0485510A4 (en) 1992-11-04
JPH04503328A (ja) 1992-06-18
WO1991001995A1 (fr) 1991-02-21
JPH087187B2 (ja) 1996-01-29
EP0485510A1 (fr) 1992-05-20
US4983268A (en) 1991-01-08
CA2060314C (fr) 1994-09-06

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Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed
MKLA Lapsed

Effective date: 19990803