CA2138948A1 - Clonage moleculaire des genes de la production de collagenase par clostridium histolyticum - Google Patents

Clonage moleculaire des genes de la production de collagenase par clostridium histolyticum

Info

Publication number: CA2138948A1
Authority: CA; Canada
Prior art keywords: collagenase; histolyticum; lys; gly; asp
Prior art date: 1992-06-22
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002138948A

Other languages

English (en)

Inventor

Hun-Chi Lin

Shau-Ping Lei

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Trigen Inc

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-06-22

Filing date

1993-06-22

Publication date

1994-01-06

1993-06-22 Application filed by Individual filed Critical Individual

1994-01-06 Publication of CA2138948A1 publication Critical patent/CA2138948A1/fr

Status Abandoned legal-status Critical Current

Links

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AEFJNECXZCODJM-UWVGGRQHSA-N Val-Val-Gly Chemical compound CC(C)[C@H]([NH3+])C(=O)N[C@@H](C(C)C)C(=O)NCC([O-])=O AEFJNECXZCODJM-UWVGGRQHSA-N 0.000 description 1
208000000260 Warts Diseases 0.000 description 1
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150000007513 acids Chemical class 0.000 description 1
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KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
238000005349 anion exchange Methods 0.000 description 1
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108010093581 aspartyl-proline Proteins 0.000 description 1
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239000013592 cell lysate Substances 0.000 description 1
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239000003795 chemical substances by application Substances 0.000 description 1
YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
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229910000388 diammonium phosphate Inorganic materials 0.000 description 1
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FSXRLASFHBWESK-UHFFFAOYSA-N dipeptide phenylalanyl-tyrosine Natural products C=1C=C(O)C=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FSXRLASFHBWESK-UHFFFAOYSA-N 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
235000019797 dipotassium phosphate Nutrition 0.000 description 1
229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
230000008014 freezing Effects 0.000 description 1
238000007710 freezing Methods 0.000 description 1
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239000008103 glucose Substances 0.000 description 1
108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 description 1
XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
108010059898 glycyl-tyrosyl-lysine Proteins 0.000 description 1
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108010087823 glycyltyrosine Proteins 0.000 description 1
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108010064235 lysylglycine Proteins 0.000 description 1
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230000035772 mutation Effects 0.000 description 1
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108010018625 phenylalanylarginine Proteins 0.000 description 1
108010012581 phenylalanylglutamate Proteins 0.000 description 1
108010051242 phenylalanylserine Proteins 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
230000003389 potentiating effect Effects 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
102200131851 rs121912444 Human genes 0.000 description 1
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230000028327 secretion Effects 0.000 description 1
108010069117 seryl-lysyl-aspartic acid Proteins 0.000 description 1
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239000007858 starting material Substances 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
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239000005720 sucrose Substances 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
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150000003522 tetracyclines Chemical class 0.000 description 1
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238000010257 thawing Methods 0.000 description 1
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238000001890 transfection Methods 0.000 description 1
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ZIBGPFATKBEMQZ-UHFFFAOYSA-N triethylene glycol Chemical compound OCCOCCOCCO ZIBGPFATKBEMQZ-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
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108010000998 wheylin-2 peptide Proteins 0.000 description 1
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235000005074 zinc chloride Nutrition 0.000 description 1
JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Medicinal Chemistry (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
General Engineering & Computer Science (AREA)
Pharmacology & Pharmacy (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Microbiology (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Saccharide Compounds (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

CA002138948A 1992-06-22 1993-06-22 Clonage moleculaire des genes de la production de collagenase par clostridium histolyticum Abandoned CA2138948A1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US90212992A	1992-06-22	1992-06-22
US07/902,129		1992-06-22

Publications (1)

Publication Number	Publication Date
CA2138948A1 true CA2138948A1 (fr)	1994-01-06

Family

ID=25415339

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002138948A Abandoned CA2138948A1 (fr)	1992-06-22	1993-06-22	Clonage moleculaire des genes de la production de collagenase par clostridium histolyticum

Country Status (5)

Country	Link
EP (1)	EP0654084A4 (fr)
JP (1)	JPH08500970A (fr)
AU (1)	AU4644793A (fr)
CA (1)	CA2138948A1 (fr)
WO (1)	WO1994000580A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN115058443A (zh) *	2022-06-22	2022-09-16	广州市乾相生物科技有限公司	转录抑制质粒pCRISPR-nadG及其在提高产溶剂梭菌胞内还原力中的应用

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU7524994A (en) *	1993-08-12	1995-03-14	University Of Maryland	Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof
DE4408940A1 (de) *	1994-03-16	1995-10-05	Boehringer Mannheim Gmbh	Rekombinante Collagenase Typ II aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden
HUP9901188A2 (hu) *	1995-03-16	1999-08-30	Knoll Aktiengesellschaft	Új, meghatározott enzimkeverékek sejtkinyeréshez és sebkezeléshez
WO1996034093A1 (fr) *	1995-04-25	1996-10-31	Baxter International Inc.	Composition contenant de la collagenase et la chymopapaine pour isoler des hepatocytes et des cellules d'ilots pancreatiques
US5670358A (en) *	1995-10-19	1997-09-23	Baxter International Inc.	Method for inhibiting chymopapain and papain enzyme activity with polysaccharides of animal origin
CA2272244A1 (fr) *	1996-11-19	1998-05-28	Roche Diagnostics Gmbh	Collagenase recombinee type i provenant du clostridium histolyticum, et son utilisation pour l'isolation des cellules et des agglomerations cellulaires
US6280993B1 (en) *	1999-08-24	2001-08-28	Ichiro Yamato	Gene encoding class I collagenase
US7811560B2 (en) *	2006-01-30	2010-10-12	Auxilium Us Holdings, Llc	Compositions and methods for treating collagen-mediated diseases
US20090191608A1 (en) *	2008-01-22	2009-07-30	Baylor Research Institute	Pancreatic Islet Cell Preparation and Transplantation
IT1397646B1 (it) *	2009-12-15	2013-01-18	Seidita	Collagenasi ricombinanti di c. histolyticum e metodo per la loro produzione.
CN102174548B (zh) *	2011-03-01	2012-07-11	山东大学	一种深海适冷耐盐胶原蛋白酶及其编码基因myr02与应用
CA2861033C (fr)	2012-01-12	2017-07-25	Auxilium International Holdings, Inc.	Enzymes de clostridium histolyticum et procedes pour les utiliser
ES3036011T3 (en)	2017-03-01	2025-09-11	Endo Operations Ltd	Apparatus and method for assessing and treating cellulite
KR20240001279A (ko)	2017-03-28	2024-01-03	엔도 벤쳐즈 리미티드	개선된 콜라게나제 생성 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5177017A (en) *	1990-03-22	1993-01-05	Trigen, Inc.	Molecular cloning of the genes responsible for collagenase production from Clostridium histolyticum

1993
- 1993-06-22 CA CA002138948A patent/CA2138948A1/fr not_active Abandoned
- 1993-06-22 AU AU46447/93A patent/AU4644793A/en not_active Abandoned
- 1993-06-22 WO PCT/US1993/005944 patent/WO1994000580A1/fr not_active Ceased
- 1993-06-22 EP EP93916670A patent/EP0654084A4/fr not_active Withdrawn
- 1993-06-22 JP JP6502521A patent/JPH08500970A/ja active Pending

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN115058443A (zh) *	2022-06-22	2022-09-16	广州市乾相生物科技有限公司	转录抑制质粒pCRISPR-nadG及其在提高产溶剂梭菌胞内还原力中的应用

Also Published As

Publication number	Publication date
JPH08500970A (ja)	1996-02-06
EP0654084A4 (fr)	1996-07-03
EP0654084A1 (fr)	1995-05-24
AU4644793A (en)	1994-01-24
WO1994000580A1 (fr)	1994-01-06

Legal Events

Date	Code	Title	Description
1997-06-23	FZDE	Dead

Publication	Publication Date	Title
KR100566136B1 (ko)	2006-11-10	효소적으로 활성인 재조합 카르복시펩티다제 b의 생산
JP3043803B2 (ja)	2000-05-22	融合タンパク質、その調製及び用途
JP2659530B2 (ja)	1997-09-30	ストレプトアビジン様ポリペプチドの製造
CA2138948A1 (fr)	1994-01-06	Clonage moleculaire des genes de la production de collagenase par clostridium histolyticum
HU204097B (en)	1991-11-28	Process for producing cloning system relating to kluyveromyces species
HU205386B (en)	1992-04-28	Process for expressing cloned lysostaphin gene and for producing dna fragment containing the gene, expression vector and transformed host cell
US4828988A (en)	1989-05-09	Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
CA1300534C (fr)	1992-05-12	Elastase pancreatique humaine
IE832921L (en)	1984-06-12	Microbial expression vector
US5516685A (en)	1996-05-14	Isolation and characterization of a novel protease from Streptomyces lividans
CA1338469C (fr)	1996-07-23	Clonage pour l'expression de la ligninase
EP0157604B1 (fr)	1992-12-30	Elastase pancréatique du porc
JPH02150282A (ja)	1990-06-08	修飾ヒトｐｓｔｉ
JP2890168B2 (ja)	1999-05-10	新規アミノペプチダーゼ
JPH03219880A (ja)	1991-09-27	細菌コラゲナーゼ遺伝子
RU2143492C1 (ru)	1999-12-27	Рекомбинантная плазмида, кодирующая гибридный белок - предшественник инсулина человека (варианты), штамм бактерий e.coli - продуцент гибридного белка - предшественника инсулина человека (варианты), способ получения инсулина человека
JPS62158487A (ja)	1987-07-14	突然変異体コ−デイング配列
US5952463A (en)	1999-09-14	Proteinase inhibitor and gene encoding the inhibitor
KR100190143B1 (ko)	1999-06-01	터닙 모자이크 바이러스 c5로부터 분리한 프로테아제
JPS6368087A (ja)	1988-03-26	蛋白質の発現と分泌に関与する領域を含むｄｎａ
JP3044325B2 (ja)	2000-05-22	グルコシルトランスフェラーゼ遺伝子及びそれを用いたグルコシルトランスフェラーゼの製造法
CA1324093C (fr)	1993-11-09	Fragment de ricin a recombinant et toxine de ricin
JP3089287B2 (ja)	2000-09-18	う蝕ワクチンの製造法
JPH0648984B2 (ja)	1994-06-29	遺伝子
JPH0675509B2 (ja)	1994-09-28	ヒト・膵臓エラスタ−ゼ▲ｉｉｉ▼ｂ

CA2138948A1 - Clonage moleculaire des genes de la production de collagenase par clostridium histolyticum - Google Patents

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Applications Claiming Priority (2)

Publications (1)

Family

ID=25415339

Family Applications (1)

Country Status (5)

Cited By (1)

Families Citing this family (14)

Family Cites Families (1)

Cited By (1)

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