CA2336249C - Nouvelle sequence d'acide nucleique de ciblage plastidial, sequence de .beta.-amylase, promoteur recepteur reagissant aux stimuli et leurs utilisations - Google Patents

Nouvelle sequence d'acide nucleique de ciblage plastidial, sequence de .beta.-amylase, promoteur recepteur reagissant aux stimuli et leurs utilisations Download PDF

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Publication number
CA2336249C
CA2336249C CA2336249A CA2336249A CA2336249C CA 2336249 C CA2336249 C CA 2336249C CA 2336249 A CA2336249 A CA 2336249A CA 2336249 A CA2336249 A CA 2336249A CA 2336249 C CA2336249 C CA 2336249C
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Prior art keywords
nucleic acid
sequence
plant
amylase
seq
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Expired - Fee Related
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CA2336249A
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CA2336249A1 (fr
Inventor
Thomas Anthony Kavanagh
Nga Thi Lao
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British American Tobacco Investments Ltd
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Advanced Technologies Cambridge Ltd
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Priority claimed from GBGB9817963.3A external-priority patent/GB9817963D0/en
Priority claimed from GBGB9817959.1A external-priority patent/GB9817959D0/en
Priority claimed from GBGB9913014.8A external-priority patent/GB9913014D0/en
Application filed by Advanced Technologies Cambridge Ltd filed Critical Advanced Technologies Cambridge Ltd
Publication of CA2336249A1 publication Critical patent/CA2336249A1/fr
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Publication of CA2336249C publication Critical patent/CA2336249C/fr
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne une nouvelle séquence de .beta.-amylase (ct .beta.-amylase) ciblant un nouveau chloroplaste, un nouveau chloroplaste ciblant une séquence d'acide nucléique, et une nouvelle séquence de .beta.-amylase. Cette invention concerne également un promoteur inductible stimulé individuellement par des stimuli tels que la lumière ou le sucre. En outre, cette invention concerne des procédés de transformation de plantes utilisant ces séquences, des cellules végétales transformées, des plantes transformées et leurs graines, ainsi que des gènes chimères comprenant ces séquences. Par ailleurs, cette invention permet d'effectuer la modification des taux d'amidon chez les plantes, le ciblage de gènes des voies de dégradation ou d'amidon biosynthétique, la résistance aux maladies ou aux parasites, ou encore la variation de l'expression génique en raison des stimuli.
CA2336249A 1998-08-19 1999-08-13 Nouvelle sequence d'acide nucleique de ciblage plastidial, sequence de .beta.-amylase, promoteur recepteur reagissant aux stimuli et leurs utilisations Expired - Fee Related CA2336249C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB9817963.3A GB9817963D0 (en) 1998-08-19 1998-08-19 A plastid-targeting nucleic acid sequence and uses therefor
GB9817959.1 1998-08-19
GB9817963.3 1998-08-19
GBGB9817959.1A GB9817959D0 (en) 1998-08-19 1998-08-19 A novel plastid-targeting nucleic acid sequence and a novel B-amylase sequence and uses therefor
GB9913014.8 1999-06-05
GBGB9913014.8A GB9913014D0 (en) 1999-06-05 1999-06-05 A plastid-targeting nucleic acid sequence,a stimulus-responsive promoter,and uses thereof
PCT/GB1999/002697 WO2000011144A2 (fr) 1998-08-19 1999-08-13 NOUVELLE SEQUENCE D'ACIDE NUCLEIQUE DE CIBLAGE DE CYTOPLASME, NOUVELLE SEQUENCE DE β-AMYLASE, PROMOTEUR RECEPTEUR REAGISSANT AUX STIMULI ET LEURS UTILISATIONS

Publications (2)

Publication Number Publication Date
CA2336249A1 CA2336249A1 (fr) 2000-03-02
CA2336249C true CA2336249C (fr) 2011-03-08

Family

ID=27269441

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CA2336249A Expired - Fee Related CA2336249C (fr) 1998-08-19 1999-08-13 Nouvelle sequence d'acide nucleique de ciblage plastidial, sequence de .beta.-amylase, promoteur recepteur reagissant aux stimuli et leurs utilisations

Country Status (18)

Country Link
US (2) US6489540B1 (fr)
EP (1) EP1105468B1 (fr)
JP (2) JP2002523040A (fr)
CN (2) CN1234868C (fr)
AT (1) ATE527346T1 (fr)
AU (1) AU773492B2 (fr)
BR (1) BR9914299A (fr)
CA (1) CA2336249C (fr)
CZ (1) CZ303574B6 (fr)
ES (1) ES2375034T3 (fr)
HU (1) HU228696B1 (fr)
MX (1) MXPA01001815A (fr)
NZ (1) NZ509642A (fr)
PL (1) PL205558B1 (fr)
SK (1) SK288125B6 (fr)
TR (1) TR200100625T2 (fr)
WO (1) WO2000011144A2 (fr)
ZA (1) ZA200100736B (fr)

Families Citing this family (15)

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JP3546337B2 (ja) * 1993-12-21 2004-07-28 ゼロックス コーポレイション 計算システム用ユーザ・インタフェース装置及びグラフィック・キーボード使用方法
NZ509642A (en) 1998-08-19 2003-08-29 Cambridge Advanced Tech A novel plastid-targeting nucleic acid sequence, a novel beta-amylase sequence, a stimulus-responsive promoter and uses thereof
WO2002067662A1 (fr) 2001-02-22 2002-09-06 Pioneer Hi-Bred International, Inc. Manipulation de genes de sucrose synthase pour ameliorer la qualite de la tige et des grains
JP2004535789A (ja) * 2001-04-25 2004-12-02 マックス−プランク−ゲゼルシャフト ツール フォルデルング デル ヴィッセンシャフテン エー．ファウ． デンプン分解酵素をコードする核酸分子
KR20040029122A (ko) * 2001-08-27 2004-04-03 신젠타 파티서페이션즈 아게 자가-프로세싱 식물 및 식물 부분
EP1562444B2 (fr) * 2002-11-08 2017-10-11 Bayer CropScience AG Procede de reduction de la teneur en acrylamide d'aliments thermo-traites
JP2007151435A (ja) * 2005-12-02 2007-06-21 Niigata Univ デンプン集積能力の高い形質転換植物およびその製造方法
CA2704016A1 (fr) * 2007-11-05 2009-05-14 Syngenta Participations Ag Methodes destinees a augmenter la teneur en amidon de vegetaux
WO2010099134A1 (fr) * 2009-02-25 2010-09-02 Syngenta Participations Ag Procédés destinés à augmenter la teneur en amidon dans la rafle d'une plante
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
CA2803868A1 (fr) 2010-06-25 2011-12-29 Agrivida, Inc. Plantes a niveaux modifies d'amidon vegetal
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
WO2015021600A1 (fr) * 2013-08-13 2015-02-19 Danisco Us Inc. Bêta-amylase et ses méthodes d'utilisation
CN104232681B (zh) * 2014-09-28 2017-02-15 浙江大学 一种植物表达载体及在制备磷酸化改性水稻淀粉中的应用
CN117481005B (zh) * 2023-09-21 2024-08-02 中国烟草总公司广东省公司 一种坡地烟与山旱稻双优融合的种植方法

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5391725A (en) * 1989-12-08 1995-02-21 New York University Medical Center Organ-specific plant promoter sequences
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5349123A (en) * 1990-12-21 1994-09-20 Calgene, Inc. Glycogen biosynthetic enzymes in plants
ATE212670T1 (de) 1990-06-18 2002-02-15 Monsanto Technology Llc Erhöhter stärkegehalt in pflanzen
US5750876A (en) * 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
GB9522589D0 (en) * 1995-11-03 1996-01-03 Innes John Centre Modified plants and plant products
BRPI9706706B1 (pt) 1996-09-03 2015-08-18 Bayer Cropscience Nv Dna, proteína de barstar, e, uso do dna
NZ509642A (en) * 1998-08-19 2003-08-29 Cambridge Advanced Tech A novel plastid-targeting nucleic acid sequence, a novel beta-amylase sequence, a stimulus-responsive promoter and uses thereof

Also Published As

Publication number Publication date
WO2000011144A2 (fr) 2000-03-02
JP2010131029A (ja) 2010-06-17
AU773492B2 (en) 2004-05-27
WO2000011144A3 (fr) 2000-09-08
EP1105468B1 (fr) 2011-10-05
US6489540B1 (en) 2002-12-03
US20030074690A1 (en) 2003-04-17
CA2336249A1 (fr) 2000-03-02
SK2042001A3 (en) 2002-01-07
HUP0200732A3 (en) 2004-10-28
SK288125B6 (sk) 2013-10-02
CN1323349A (zh) 2001-11-21
BR9914299A (pt) 2001-11-27
MXPA01001815A (es) 2002-06-21
NZ509642A (en) 2003-08-29
CN1234868C (zh) 2006-01-04
CZ2001460A3 (cs) 2001-11-14
HU228696B1 (en) 2013-05-28
JP2002523040A (ja) 2002-07-30
ATE527346T1 (de) 2011-10-15
CN1528904A (zh) 2004-09-15
CZ303574B6 (cs) 2012-12-19
ES2375034T3 (es) 2012-02-24
HUP0200732A2 (hu) 2002-07-29
CN1528904B (zh) 2010-05-26
JP4673431B2 (ja) 2011-04-20
ZA200100736B (en) 2002-06-26
TR200100625T2 (tr) 2001-06-21
PL346704A1 (en) 2002-02-25
PL205558B1 (pl) 2010-05-31
EP1105468A2 (fr) 2001-06-13
AU5432699A (en) 2000-03-14

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