CA2386607A1 - Method for identifying binding partners with position-specific arrays - Google Patents

Method for identifying binding partners with position-specific arrays Download PDF

Info

Publication number: CA2386607A1
Authority: CA; Canada
Prior art keywords: further characterized; array; carrier material; bound; cell lysate
Prior art date: 1999-09-03
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002386607A

Other languages

English (en)

French (fr)

Inventor

Eberhard Krause

Thomas Bittorf

Jens Schneider-Mergener

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Jerini AG

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-09-03

Filing date

2000-09-01

Publication date

2001-03-15

2000-09-01 Application filed by Individual filed Critical Individual

2001-03-15 Publication of CA2386607A1 publication Critical patent/CA2386607A1/en

Status Abandoned legal-status Critical Current

Links

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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
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- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

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Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Chemical & Material Sciences (AREA)
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Bioinformatics & Cheminformatics (AREA)
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Urology & Nephrology (AREA)
Hematology (AREA)
Analytical Chemistry (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Medicinal Chemistry (AREA)
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Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Spectroscopy & Molecular Physics (AREA)
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Pathology (AREA)
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Chemical Kinetics & Catalysis (AREA)
Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CA002386607A 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays Abandoned CA2386607A1 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
DE19943743.2		1999-09-03
DE19943743A DE19943743C2 (de)	1999-09-03	1999-09-03	Verfahren zur Identifizierung von Bindungspartnern mit positions-spezifischen Arrays
PCT/DE2000/003071 WO2001018545A2 (de)	1999-09-03	2000-09-01	Verfahren zur identifizierung von bindungspartnern mit positions-spezifischen arrays

Publications (1)

Publication Number	Publication Date
CA2386607A1 true CA2386607A1 (en)	2001-03-15

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Application Number	Title	Priority Date	Filing Date
CA002386607A Abandoned CA2386607A1 (en)	1999-09-03	2000-09-01	Method for identifying binding partners with position-specific arrays

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EP (1)	EP1212622A2 (de)
JP (1)	JP2003511652A (de)
AU (1)	AU7644200A (de)
CA (1)	CA2386607A1 (de)
CZ (1)	CZ2002660A3 (de)
DE (2)	DE19943743C2 (de)
HU (1)	HUP0203074A2 (de)
PL (1)	PL353995A1 (de)
WO (1)	WO2001018545A2 (de)

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DE10141464A1 (de) *	2001-08-23	2004-03-04	Genprofile Ag I.Ins.	Sequenzvarianten des für neuropsychiatrische Krankheiten verantwortlichen Gens 22444 und deren Verwendung
EP1319954A1 (de) *	2001-12-12	2003-06-18	Centre National de Genotypage	Verfahren zur Proteinanalyse durch Protein bindende Arrays
US7504364B2 (en)	2002-03-01	2009-03-17	Receptors Llc	Methods of making arrays and artificial receptors
US20030228709A1 (en) *	2002-03-25	2003-12-11	Kozlowski Roland Zbignieiw	Arrays
CN1665790A (zh)	2002-05-03	2005-09-07	莫莱丘莱尔探针公司	用于检测和分离磷酸化分子的组合物及方法
US7445894B2 (en)	2002-05-03	2008-11-04	Molecular Probes, Inc.	Compositions and methods for detection and isolation of phosphorylated molecules
US7469076B2 (en)	2003-09-03	2008-12-23	Receptors Llc	Sensors employing combinatorial artificial receptors
EP1613737A4 (de)	2003-03-28	2008-12-03	Receptors Llc	Reversibel immobilisierte bausteine beinhaltend künstliche rezeptoren und verfahren
US20040248323A1 (en)	2003-06-09	2004-12-09	Protometrix, Inc.	Methods for conducting assays for enzyme activity on protein microarrays
US7884052B2 (en)	2004-09-03	2011-02-08	Receptors Llc	Combinatorial artificial receptors including tether building blocks on scaffolds
EP1789792A2 (de)	2004-09-11	2007-05-30	Receptors LLC	Kombinatorische künstliche rezeptoren mit peptidbausteinen

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DE4027675C2 (de) *	1990-08-31	1997-05-07	Biotechnolog Forschung Gmbh	Verfahren zur parallelen Herstellung von trägergebundenen oder freien Peptiden, trägergebundene Peptide und ihre Verwendung
CA2095633C (en) *	1990-12-03	2003-02-04	Lisa J. Garrard	Enrichment method for variant proteins with altered binding properties
US5834318A (en) *	1995-05-10	1998-11-10	Bayer Corporation	Screening of combinatorial peptide libraries for selection of peptide ligand useful in affinity purification of target proteins
WO1997043301A2 (en) *	1996-05-10	1997-11-20	Novartis Ag	Identification of members of combinatorial libraries by mass spectrometry
US6207370B1 (en) *	1997-09-02	2001-03-27	Sequenom, Inc.	Diagnostics based on mass spectrometric detection of translated target polypeptides
GB9800378D0 (en) *	1998-01-08	1998-03-04	Univ Liverpool	Proteome analysis

1999
- 1999-09-03 DE DE19943743A patent/DE19943743C2/de not_active Expired - Fee Related
2000
- 2000-09-01 DE DE10082703T patent/DE10082703D2/de not_active Expired - Lifetime
- 2000-09-01 CA CA002386607A patent/CA2386607A1/en not_active Abandoned
- 2000-09-01 AU AU76442/00A patent/AU7644200A/en not_active Abandoned
- 2000-09-01 WO PCT/DE2000/003071 patent/WO2001018545A2/de not_active Ceased
- 2000-09-01 EP EP00965834A patent/EP1212622A2/de not_active Withdrawn
- 2000-09-01 CZ CZ2002660A patent/CZ2002660A3/cs unknown
- 2000-09-01 JP JP2001522083A patent/JP2003511652A/ja active Pending
- 2000-09-01 HU HU0203074A patent/HUP0203074A2/hu unknown
- 2000-09-01 PL PL00353995A patent/PL353995A1/xx not_active Application Discontinuation

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Publication number	Publication date
WO2001018545A2 (de)	2001-03-15
AU7644200A (en)	2001-04-10
DE19943743A1 (de)	2001-03-15
EP1212622A2 (de)	2002-06-12
DE19943743C2 (de)	2002-02-07
WO2001018545A3 (de)	2001-09-13
DE10082703D2 (de)	2002-02-14
CZ2002660A3 (cs)	2002-10-16
JP2003511652A (ja)	2003-03-25
PL353995A1 (en)	2003-12-15
HUP0203074A2 (hu)	2002-12-28

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Date	Code	Title	Description
2005-09-01	FZDE	Discontinued

Publication	Publication Date	Title
Annan et al.	2001	A multidimensional electrospray MS-based approach to phosphopeptide mapping
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