CL2011000665A1 - Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas. - Google Patents

Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas.

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Publication number
CL2011000665A1
CL2011000665A1 CL2011000665A CL2011000665A CL2011000665A1 CL 2011000665 A1 CL2011000665 A1 CL 2011000665A1 CL 2011000665 A CL2011000665 A CL 2011000665A CL 2011000665 A CL2011000665 A CL 2011000665A CL 2011000665 A1 CL2011000665 A1 CL 2011000665A1
Authority
CL
Chile
Prior art keywords
nucleic acid
acid molecule
ahasl
brassica
protein
Prior art date
Application number
CL2011000665A
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English (en)
Inventor
Christian Schopke
Bijay Singh
Peter Beetham
Dale Carlson
Greg Gocal
Elver John Mc
James Pearce
Keith Walker
Original Assignee
Basf Agrochemical Products Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Agrochemical Products Bv filed Critical Basf Agrochemical Products Bv
Publication of CL2011000665A1 publication Critical patent/CL2011000665A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Catching Or Destruction (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MOLÉCULA DE ÁCIDO NUCLEICO RECOMBINANTE/MUTAGENIZADA, QUE CODIFICA UNA PROTEÍNA DE SUBUNIDAD MAYOR DE ACETOHIDROXIÁCIDO SINTASA 111 (AHASL) DE BRASSICA NAPUS QUE COMPRENDE UNA SUSTITUCIÓN DE ALANINA POR TREONINA EN LA POSICIÓN A122 DE LA 5EQ ID NO:23 (A122T) Y UNA SUSTITUCIÓN DE SERINA POR ASPARAGINA EN LA POSICIÓN 5653 DE LA 5EQ ID NO:23 (5653N), DONDE DICHA AHASL ES RESISTENTE A LA INHIBICIÓN POR UN HERBICIDA DE IMIDAZOLINONA, Y DONDE LA PROTEÍNA AHASL DEMUESTRA UN NIVEL SINÉRGICO DE RESISTENCIA A LA INHIBICIÓN POR HERBICIDA DE IMIDAZOLINONA COMPARADO CON LOS<br /> NIVELES ADITIVOS DE LA PROTEÍNA DE AHASL 111 DE BRASSLCA NAPUS QUE TIENE UNA ÚNICA MUTACIÓN EN A122T O EN S653N, RESPECTIVAMENTE. VECTOR DE EXPRESIÓN QUE COMPRENDE A DICHA MOLÉCULA DE ÁCIDO NUCLEICO. MÉTODO DE CONTROL DE MALEZAS EN LA VECINDAD DE UNA PLANTA DE BRASSICA QUE COMPRENDE EN SU GENOMA A LA MOLÉCULA DE ÁCIDO NUCLEICO DE AHASL MUTAGENIZADA. MÉTODO DE REPRODUCCIÓN DE DICHA PLANTA DE BRASSICA. CÉLULA DE PLANTA, QUE COMPRENDE A DICHA MOLÉCULA DE ÁCIDO NUCLEICO, O DE CUALQUIERA DE LAS LINEAS DE PLANTAS BNCL131A1, BNCL140B3, BNCL140C7, O PM1PM2/BNCL131AL,<br /> O QUE COMPRENDE LAS CARACTERÍSTICAS DE RESISTENCIA A HERBICIDAS, DE DICHAS LÍNEAS DE PLANTAS. MÉTODO PARA CONTROLAR MALEZAS, QUE UTILIZA A DICHA PLANTA DE BRASSICA. MÉTODO PARA IDENTIFICAR UNA MOLÉCULA DE ÁCIDO NUCLEICO AHAS MUTAGENIZADA EN UNA MUESTRA BIOLOGICA.<br />  
CL2011000665A 2008-09-26 2011-03-25 Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas. CL2011000665A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10054108P 2008-09-26 2008-09-26

Publications (1)

Publication Number Publication Date
CL2011000665A1 true CL2011000665A1 (es) 2011-08-12

Family

ID=41351460

Family Applications (1)

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CL2011000665A CL2011000665A1 (es) 2008-09-26 2011-03-25 Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas.

Country Status (20)

Country Link
US (1) US20120060243A1 (es)
EP (3) EP2342331B1 (es)
JP (2) JP6133011B2 (es)
KR (1) KR20110080154A (es)
CN (2) CN103834675A (es)
AP (1) AP2011005671A0 (es)
AR (1) AR073402A1 (es)
AU (2) AU2009296625B2 (es)
BR (1) BRPI0919380A2 (es)
CA (1) CA2737939C (es)
CL (1) CL2011000665A1 (es)
EA (2) EA025732B9 (es)
GE (1) GEP20146179B (es)
MX (1) MX2011003266A (es)
NZ (2) NZ592464A (es)
PL (2) PL2342331T3 (es)
UA (1) UA115019C2 (es)
UY (1) UY32144A (es)
WO (3) WO2010036771A2 (es)
ZA (1) ZA201102076B (es)

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CN110545659A (zh) * 2017-01-31 2019-12-06 赖斯泰克有限公司 多个突变对提高水稻中的除草剂抗性/耐受性的作用
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JP2019141045A (ja) * 2019-03-04 2019-08-29 アドヴァンタ・ホールディングス・ベスローテン・フェンノートシャップ 突然変異アセトヒドロキシ酸合成酵素タンパク質のラージサブユニットをコードする突然変異ポリヌクレオチドを有し、除草剤耐性が増大したソルガム植物
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