CS269959B2 - Method and agent of alpha-amylase determination - Google Patents

Method and agent of alpha-amylase determination Download PDF

Info

Publication number: CS269959B2
Authority: CS; Czechoslovakia
Prior art keywords: amylase; glucosidase; alpha; determination; substrate
Prior art date: 1983-06-28

Application number

CS844827A

Other languages

Czech (cs)

English (en)

Other versions

CS482784A2 (en

Inventor

Eberhard Dr Henkel

Barkew Dr Dolabdjian

Roland Dr Helger

Rainer Dr Klink

Uwe Dr Wurzburg

Original Assignee

Merck Patent Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-06-28

Filing date

1984-06-25

Publication date

1990-05-14

1984-06-25 Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh

1989-09-12 Publication of CS482784A2 publication Critical patent/CS482784A2/cs

1990-05-14 Publication of CS269959B2 publication Critical patent/CS269959B2/cs

Links

238000000034 method Methods 0.000 title claims description 13
239000003795 chemical substances by application Substances 0.000 title claims description 9
108090000637 alpha-Amylases Proteins 0.000 title description 12
102000004139 alpha-Amylases Human genes 0.000 title description 12
229940024171 alpha-amylase Drugs 0.000 title description 12
239000000758 substrate Substances 0.000 claims description 22
239000004382 Amylase Substances 0.000 claims description 19
239000003153 chemical reaction reagent Substances 0.000 claims description 16
102100024295 Maltase-glucoamylase Human genes 0.000 claims description 9
108010028144 alpha-Glucosidases Proteins 0.000 claims description 9
-1 4-nitrophenyl- Chemical group 0.000 claims description 8
238000003776 cleavage reaction Methods 0.000 claims description 8
230000007017 scission Effects 0.000 claims description 8
239000000872 buffer Substances 0.000 claims description 7
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 6
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
230000003213 activating effect Effects 0.000 claims description 4
230000002255 enzymatic effect Effects 0.000 claims description 4
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
239000001103 potassium chloride Substances 0.000 claims description 2
235000011164 potassium chloride Nutrition 0.000 claims description 2
230000008569 process Effects 0.000 claims description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
239000011780 sodium chloride Substances 0.000 claims description 2
102000006995 beta-Glucosidase Human genes 0.000 description 9
108010047754 beta-Glucosidase Proteins 0.000 description 9
230000008033 biological extinction Effects 0.000 description 9
238000003556 assay Methods 0.000 description 5
230000008859 change Effects 0.000 description 5
238000006243 chemical reaction Methods 0.000 description 5
230000000694 effects Effects 0.000 description 5
229910052736 halogen Inorganic materials 0.000 description 5
150000002367 halogens Chemical group 0.000 description 5
239000000203 mixture Substances 0.000 description 5
BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 3
PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 3
238000010521 absorption reaction Methods 0.000 description 3
108010019077 beta-Amylase Proteins 0.000 description 3
229910052801 chlorine Inorganic materials 0.000 description 3
239000000460 chlorine Substances 0.000 description 3
PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 3
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
125000000636 p-nitrophenyl group Chemical class [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 3
239000008363 phosphate buffer Substances 0.000 description 3
239000000243 solution Substances 0.000 description 3
IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 2
102000013142 Amylases Human genes 0.000 description 2
108010065511 Amylases Proteins 0.000 description 2
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
108010044467 Isoenzymes Proteins 0.000 description 2
229920002472 Starch Polymers 0.000 description 2
235000019418 amylase Nutrition 0.000 description 2
238000000354 decomposition reaction Methods 0.000 description 2
238000001514 detection method Methods 0.000 description 2
239000008103 glucose Substances 0.000 description 2
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
125000006501 nitrophenyl group Chemical group 0.000 description 2
229920001542 oligosaccharide Polymers 0.000 description 2
150000002482 oligosaccharides Chemical class 0.000 description 2
239000008107 starch Substances 0.000 description 2
235000019698 starch Nutrition 0.000 description 2
239000000126 substance Substances 0.000 description 2
125000001424 substituent group Chemical group 0.000 description 2
238000006467 substitution reaction Methods 0.000 description 2
210000002700 urine Anatomy 0.000 description 2
FSRMLWKBCQFLJA-HTZQGQHSSA-N (2r,3r,4s,5s,6r)-2-[(2r,3s,4r,5r,6r)-6-[(2r,3s,4r,5r,6r)-6-[(2r,3s,4r,5r,6r)-6-[(2r,3s,4r,5r,6r)-6-[(2r,3s,4r,5r,6r)-6-[(2r,3s,4r,5r,6r)-4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-4,5-dihyd Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](O[C@H](O[C@@H]3[C@H](O[C@H](O[C@@H]4[C@H](O[C@H](O[C@@H]5[C@H](O[C@H](O[C@@H]6[C@H](O[C@H](OC=7C=CC(=CC=7)[N+]([O-])=O)[C@H](O)[C@H]6O)CO)[C@H](O)[C@H]5O)CO)[C@H](O)[C@H]4O)CO)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FSRMLWKBCQFLJA-HTZQGQHSSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 description 1
BOFRXDMCQRTGII-UHFFFAOYSA-N 619-08-9 Chemical compound OC1=CC=C([N+]([O-])=O)C=C1Cl BOFRXDMCQRTGII-UHFFFAOYSA-N 0.000 description 1
WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
102000004366 Glucosidases Human genes 0.000 description 1
108010056771 Glucosidases Proteins 0.000 description 1
XYZZKVRWGOWVGO-UHFFFAOYSA-N Glycerol-phosphate Chemical compound OP(O)(O)=O.OCC(O)CO XYZZKVRWGOWVGO-UHFFFAOYSA-N 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
208000016222 Pancreatic disease Diseases 0.000 description 1
GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
239000002250 absorbent Substances 0.000 description 1
230000002745 absorbent Effects 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000009471 action Effects 0.000 description 1
239000012190 activator Substances 0.000 description 1
230000008901 benefit Effects 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
239000010839 body fluid Substances 0.000 description 1
GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
229910052794 bromium Inorganic materials 0.000 description 1
125000001309 chloro group Chemical group Cl* 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
230000005593 dissociations Effects 0.000 description 1
230000002183 duodenal effect Effects 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
229910052731 fluorine Inorganic materials 0.000 description 1
239000011737 fluorine Substances 0.000 description 1
GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
230000001771 impaired effect Effects 0.000 description 1
230000006698 induction Effects 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
PLXPTFQGYWXIEA-UHFFFAOYSA-N nitroformonitrile Chemical compound [O-][N+](=O)C#N PLXPTFQGYWXIEA-UHFFFAOYSA-N 0.000 description 1
229940031826 phenolate Drugs 0.000 description 1
210000002381 plasma Anatomy 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
210000003296 saliva Anatomy 0.000 description 1
239000000523 sample Substances 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
238000002798 spectrophotometry method Methods 0.000 description 1
230000007704 transition Effects 0.000 description 1
150000008495 β-glucosides Chemical class 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CS844827A 1983-06-28 1984-06-25 Method and agent of alpha-amylase determination CS269959B2 (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19833323245 DE3323245A1 (de)	1983-06-28	1983-06-28	Verfahren und reagenz zur bestimmung von (alpha)-amylase

Publications (2)

Publication Number	Publication Date
CS482784A2 CS482784A2 (en)	1989-09-12
CS269959B2 true CS269959B2 (en)	1990-05-14

Family

ID=6202584

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CS844827A CS269959B2 (en)	1983-06-28	1984-06-25	Method and agent of alpha-amylase determination

Country Status (7)

Country	Link
US (2)	US4698300A (ja)
EP (1)	EP0133329B1 (ja)
JP (1)	JPS6062999A (ja)
CS (1)	CS269959B2 (ja)
DE (2)	DE3323245A1 (ja)
IL (1)	IL72226A (ja)
ZA (1)	ZA844952B (ja)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3413118A1 (de) *	1984-04-06	1985-10-24	Miles Laboratories, Inc., Elkhart, Ind.	Analysenverfahren und mittel zum nachweis esterolytischer und/oder proteolytischer enzyme
JPS6128400A (ja) *	1984-07-19	1986-02-08	Toyobo Co Ltd	α−アミラ−ゼ活性測定法および測定用試薬
US4649108A (en) *	1984-07-26	1987-03-10	Genzyme Corporation	Alpha amylase assay
JPS61112092A (ja) *	1984-11-07	1986-05-30	Meiji Seika Kaisha Ltd	新規グルコサミン誘導体及びこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼの活性測定試薬
DE3525926A1 (de) *	1985-07-19	1987-01-29	Boehringer Mannheim Gmbh	Verfahren und reagenz zur spezifischen bestimmung von pankreas-alpha-amylase
EP0259521A1 (en) *	1986-09-10	1988-03-16	Akzo N.V.	Test reagent for amylase determination
US5158872A (en) *	1986-10-07	1992-10-27	Hoechst Celanese Corporation	Aromatic substituted glycoside
US4963479A (en) *	1986-10-07	1990-10-16	Hoechst Celanese Corporation	Reagent system for an alpha-amylase assay containing aromatic substituted glycoside
JPH0612999B2 (ja) *	1986-11-17	1994-02-23	関東化学株式会社	塩素イオン定量用試薬
US5229270A (en) *	1986-11-17	1993-07-20	Kanto Kagaku Kabushiki Kaisha	Reagent for the determination of chlorine ion
IL85581A (en) *	1987-05-21	1992-05-25	Technicon Instr	Substrate for beta-galactosidase comprising derivatives of 4-nitrophenyl-beta-d-galactopyranoside and beta-galactosidase immunoassay containing said substrate
AU621245B2 (en) *	1989-10-17	1992-03-05	Boehringer Mannheim Gmbh	Hydrolase substrates, a process for the preparation thereof and agents containing them
JP2886249B2 (ja) *	1990-03-14	1999-04-26	日本食品化工株式会社	ガラクトシル―マルトオリゴ糖誘導体、その製造法およびα―アミラーゼ活性測定方法
CA2039564A1 (en) *	1990-04-10	1991-10-11	James R. Rasmussen	Oligosaccharide compounds and preparation thereof
DE4021063A1 (de) *	1990-07-03	1992-01-09	Boehringer Mannheim Gmbh	Neue ss-galactosidase-substrate fuer den cedia
JP2770891B2 (ja) *	1991-06-26	1998-07-02	キッコーマン株式会社	マルトオリゴシド誘導体、これを有効成分とするα‐アミラーゼ活性測定用試薬及びこれを用いたα‐アミラーゼ活性の測定方法
JP3075377B2 (ja) *	1991-11-06	2000-08-14	東洋紡績株式会社	α−アミラーゼ活性の測定法およびα−アミラーゼ活性測定用試薬
US5766872A (en) *	1995-11-20	1998-06-16	Dade International Inc.	Method for eliminating hemolysis interference in an amylase analysis
US20080050451A1 (en) *	2006-06-16	2008-02-28	Mabry Helen C	Methods for assessing dehydration and shock, assays and kits for the methods
WO2010090810A2 (en) *	2009-02-05	2010-08-12	Hydradx, Inc.	Diagnostic device and method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4145527A (en) *	1976-07-13	1979-03-20	E. I. Du Pont De Nemours And Company	Nitro aromatic glycosides
US4102747A (en) *	1977-07-28	1978-07-25	American Hospital Supply Corporation	Amylase determination
DE2755803A1 (de) *	1977-12-14	1979-06-21	Boehringer Mannheim Gmbh	Verfahren und reagens zur bestimmung von alpha-amylase
AT362526B (de) *	1977-09-13	1981-05-25	Boehringer Mannheim Gmbh	Verfahren und reagens zur bestimmung von alpha- -amylase
US4233403A (en) *	1978-02-27	1980-11-11	E. I. Du Pont De Nemours And Company	Amylase assay
US4225672A (en) *	1979-02-27	1980-09-30	Hall Leo M	Method for producing maltooligosaccharide glycosides
DE3328616A1 (de) *	1983-08-08	1985-02-28	Boehringer Mannheim Gmbh, 6800 Mannheim	Oligoglucosidderivate

1983
- 1983-06-28 DE DE19833323245 patent/DE3323245A1/de not_active Withdrawn
1984
- 1984-06-15 EP EP84200866A patent/EP0133329B1/de not_active Expired - Lifetime
- 1984-06-15 DE DE8484200866T patent/DE3481432D1/de not_active Expired - Lifetime
- 1984-06-25 CS CS844827A patent/CS269959B2/cs unknown
- 1984-06-26 IL IL72226A patent/IL72226A/xx unknown
- 1984-06-28 JP JP59132172A patent/JPS6062999A/ja active Granted
- 1984-06-28 ZA ZA844952A patent/ZA844952B/xx unknown
- 1984-06-28 US US06/625,482 patent/US4698300A/en not_active Expired - Lifetime
1987
- 1987-08-04 US US07/081,380 patent/US4857640A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP0133329A2 (de)	1985-02-20
US4857640A (en)	1989-08-15
US4698300A (en)	1987-10-06
DE3481432D1 (de)	1990-04-05
IL72226A0 (en)	1984-10-31
JPH059069B2 (ja)	1993-02-03
IL72226A (en)	1987-12-31
EP0133329A3 (en)	1987-10-14
DE3323245A1 (de)	1985-01-10
CS482784A2 (en)	1989-09-12
ZA844952B (en)	1985-02-27
JPS6062999A (ja)	1985-04-11
EP0133329B1 (de)	1990-02-28

Publication	Publication Date	Title
CS269959B2 (en)	1990-05-14	Method and agent of alpha-amylase determination
FI61916C (fi)	1982-10-11	Foerfarande och reagens foer bestaemning av alfa-amylas
Foo et al.	1998	Amylase measurement with 2-chloro-4-nitrophenyl maltotrioside as substrate
JPS6163299A (ja)	1986-04-01	アルフア・アミラ−ゼの検出法
US4000042A (en)	1976-12-28	Diagnostic reagent for the determination of amylase
Dupuy et al.	1987	Rapid determination of alpha-amylase activity by use of a new chromogenic substrate.
RU2122740C1 (ru)	1998-11-27	Способ определения мочевины в биологических жидкостях и набор реактивов для его осуществления
Asp	1971	Improved method for the assay of phenylglycosidase activity with a 4-aminoantipyrine reagent
EP0260414B1 (en)	1992-04-15	Method of differential assay for alpha-amylase isozymes and a kit for the same
US5766872A (en)	1998-06-16	Method for eliminating hemolysis interference in an amylase analysis
EP0243125B1 (en)	1992-08-12	Method of measuring phenol and alpha-naphthol, and method of measuring the activity of enzymes
Kovar et al.	1983	Determination of cholesterol in sera
KR920000058B1 (ko)	1992-01-06	가수분해효소 활성을 갖는 물질들의 검출방법 및 약품
US4983512A (en)	1991-01-08	Reagent for determination of acid phosphatase
Ungerer et al.	1993	An enzymatic assay of inorganic phosphate in serum using nucleoside phosphorylase and xanthine oxidase
IE47123B1 (en)	1983-12-28	Analytical device
US6818415B2 (en)	2004-11-16	Sodium activation of amylase
US4212844A (en)	1980-07-15	Rapid diagnostic composition for the determination of uric acid in body fluids
JP2000189194A (ja)	2000-07-11	α―アミラ―ゼ活性測定試薬及び測定方法
JPH02276597A (ja)	1990-11-13	体液中のカルシウム測定方法
EP0886139A1 (en)	1998-12-23	A reagent for measurement and a measurement method
Bais	1982	Evaluation of an amylase method utilizing p-nitrophenyl glucosides as substrates
JPH1014597A (ja)	1998-01-20	体液中のカルシウムイオン測定用試薬組成物および測定方法
JPH11266898A (ja)	1999-10-05	塩素イオン測定用試薬組成物
CS254978B2 (en)	1988-02-15	Agent for glucose,cholesterol and haemoglobin determination