DD149874A3 - Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase - Google Patents

Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase Download PDF

Info

Publication number: DD149874A3
Authority: DD; German Democratic Republic
Prior art keywords: enzyme; hydroxybutyrate; protein; item; acetoacetate
Prior art date: 1980-03-31

Application number

DD22006080A

Other languages

German (de)

English (en)

Inventor

Wulfdieter Schoepp

Hans-Peter Kleber

Original Assignee

Wulfdieter Schoepp

Kleber Hans Peter

Tauchert Hermann

Grunow Marlis

Stolarski Kristina

Schaefer Angelika

Harnisch Heimo

Ruede Waltraud

Trobisch Elke

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-03-31

Filing date

1980-03-31

Publication date

1981-08-05

1980-03-31 Application filed by Wulfdieter Schoepp, Kleber Hans Peter, Tauchert Hermann, Grunow Marlis, Stolarski Kristina, Schaefer Angelika, Harnisch Heimo, Ruede Waltraud, Trobisch Elke filed Critical Wulfdieter Schoepp

1980-03-31 Priority to DD22006080A priority Critical patent/DD149874A3/de

1980-12-31 Priority to SU807771547A priority patent/SU1731813A1/ru

1981-01-03 Priority to BG5029081A priority patent/BG41060A1/xx

1981-01-05 Priority to CS8981A priority patent/CS233318B1/cs

1981-03-13 Priority to DE19813109748 priority patent/DE3109748A1/de

1981-08-05 Publication of DD149874A3 publication Critical patent/DD149874A3/de

Links

238000000034 method Methods 0.000 title claims abstract description 17
102000034279 3-hydroxybutyrate dehydrogenases Human genes 0.000 title claims abstract description 11
108090000124 3-hydroxybutyrate dehydrogenases Proteins 0.000 title claims abstract description 11
238000002360 preparation method Methods 0.000 title claims abstract description 6
102000004190 Enzymes Human genes 0.000 claims abstract description 21
108090000790 Enzymes Proteins 0.000 claims abstract description 21
230000000694 effects Effects 0.000 claims abstract description 21
241000589776 Pseudomonas putida Species 0.000 claims abstract description 7
SJZRECIVHVDYJC-UHFFFAOYSA-M 4-hydroxybutyrate Chemical compound OCCCC([O-])=O SJZRECIVHVDYJC-UHFFFAOYSA-M 0.000 claims abstract description 5
WDJHALXBUFZDSR-UHFFFAOYSA-M acetoacetate Chemical compound CC(=O)CC([O-])=O WDJHALXBUFZDSR-UHFFFAOYSA-M 0.000 claims abstract description 4
229930195733 hydrocarbon Natural products 0.000 claims abstract description 3
150000002430 hydrocarbons Chemical class 0.000 claims abstract description 3
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 22
102000004169 proteins and genes Human genes 0.000 claims description 17
108090000623 proteins and genes Proteins 0.000 claims description 17
229910052921 ammonium sulfate Inorganic materials 0.000 claims description 12
235000011130 ammonium sulphate Nutrition 0.000 claims description 12
239000000287 crude extract Substances 0.000 claims description 7
241000894006 Bacteria Species 0.000 claims description 4
230000003247 decreasing effect Effects 0.000 claims description 4
239000000284 extract Substances 0.000 claims description 4
239000007788 liquid Substances 0.000 claims description 4
238000001556 precipitation Methods 0.000 claims description 4
229920002684 Sepharose Polymers 0.000 claims description 3
239000001166 ammonium sulphate Substances 0.000 claims description 3
230000001580 bacterial effect Effects 0.000 claims description 3
238000000746 purification Methods 0.000 claims description 3
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 2
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Chemical compound CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 2
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
239000004472 Lysine Substances 0.000 claims description 2
229910019142 PO4 Inorganic materials 0.000 claims description 2
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
230000006735 deficit Effects 0.000 claims description 2
235000014113 dietary fatty acids Nutrition 0.000 claims description 2
239000000194 fatty acid Substances 0.000 claims description 2
229930195729 fatty acid Natural products 0.000 claims description 2
150000004665 fatty acids Chemical class 0.000 claims description 2
150000002500 ions Chemical class 0.000 claims description 2
239000000463 material Substances 0.000 claims description 2
229910052760 oxygen Inorganic materials 0.000 claims description 2
239000001301 oxygen Substances 0.000 claims description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
239000010452 phosphate Substances 0.000 claims description 2
230000015556 catabolic process Effects 0.000 claims 2
238000006731 degradation reaction Methods 0.000 claims 2
238000012870 ammonium sulfate precipitation Methods 0.000 claims 1
239000012051 hydrophobic carrier Substances 0.000 claims 1
239000000758 substrate Substances 0.000 abstract description 6
238000012258 culturing Methods 0.000 abstract description 2
235000018102 proteins Nutrition 0.000 description 15
239000000243 solution Substances 0.000 description 12
239000013049 sediment Substances 0.000 description 5
229910052799 carbon Inorganic materials 0.000 description 4
238000005119 centrifugation Methods 0.000 description 4
239000007983 Tris buffer Substances 0.000 description 3
239000008363 phosphate buffer Substances 0.000 description 3
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
WHBMMWSBFZVSSR-UHFFFAOYSA-M 3-hydroxybutyrate Chemical compound CC(O)CC([O-])=O WHBMMWSBFZVSSR-UHFFFAOYSA-M 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
235000019750 Crude protein Nutrition 0.000 description 2
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
WHBMMWSBFZVSSR-UHFFFAOYSA-N R3HBA Natural products CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 2
241000191043 Rhodobacter sphaeroides Species 0.000 description 2
239000000126 substance Substances 0.000 description 2
229960005486 vaccine Drugs 0.000 description 2
238000005406 washing Methods 0.000 description 2
PHIQHXFUZVPYII-ZCFIWIBFSA-N (R)-carnitine Chemical compound C[N+](C)(C)C[C@H](O)CC([O-])=O PHIQHXFUZVPYII-ZCFIWIBFSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
235000019766 L-Lysine Nutrition 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
102000013460 Malate Dehydrogenase Human genes 0.000 description 1
108010026217 Malate Dehydrogenase Proteins 0.000 description 1
102000007327 Protamines Human genes 0.000 description 1
108010007568 Protamines Proteins 0.000 description 1
101710202013 Protein 1.5 Proteins 0.000 description 1
239000008272 agar Substances 0.000 description 1
238000006065 biodegradation reaction Methods 0.000 description 1
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
239000004327 boric acid Substances 0.000 description 1
239000000872 buffer Substances 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
PHIQHXFUZVPYII-UHFFFAOYSA-N carnitine Chemical compound C[N+](C)(C)CC(O)CC([O-])=O PHIQHXFUZVPYII-UHFFFAOYSA-N 0.000 description 1
229960004203 carnitine Drugs 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
238000007710 freezing Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
150000002576 ketones Chemical class 0.000 description 1
229960003136 leucine Drugs 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
230000004962 physiological condition Effects 0.000 description 1
229940048914 protamine Drugs 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000000527 sonication Methods 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
229910021653 sulphate ion Inorganic materials 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000009210 therapy by ultrasound Methods 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Compounds Of Unknown Constitution (AREA)

DD22006080A 1980-03-31 1980-03-31 Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase DD149874A3 (de)

Priority Applications (5)

Application Number	Priority Date	Filing Date	Title
DD22006080A DD149874A3 (de)	1980-03-31	1980-03-31	Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase
SU807771547A SU1731813A1 (ru)	1980-03-31	1980-12-31	Способ получени 3-гидроксибутиратдегидрогеназы
BG5029081A BG41060A1 (en)	1980-03-31	1981-01-03	Method for preparing of 3- hydroxybutyratedehydrogenase
CS8981A CS233318B1 (en)	1980-03-31	1981-01-05	Method of 3-hydroxybutyratedehydrogenase preparation
DE19813109748 DE3109748A1 (de)	1980-03-31	1981-03-13	Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DD22006080A DD149874A3 (de)	1980-03-31	1980-03-31	Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase

Publications (1)

Publication Number	Publication Date
DD149874A3 true DD149874A3 (de)	1981-08-05

Family

ID=5523436

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD22006080A DD149874A3 (de)	1980-03-31	1980-03-31	Verfahren zur herstellung von 3-hydroxybutyratdehydrogenase

Country Status (5)

Country	Link
BG (1)	BG41060A1 (cs)
CS (1)	CS233318B1 (cs)
DD (1)	DD149874A3 (cs)
DE (1)	DE3109748A1 (cs)
SU (1)	SU1731813A1 (cs)

1980
- 1980-03-31 DD DD22006080A patent/DD149874A3/de not_active IP Right Cessation
- 1980-12-31 SU SU807771547A patent/SU1731813A1/ru active
1981
- 1981-01-03 BG BG5029081A patent/BG41060A1/xx unknown
- 1981-01-05 CS CS8981A patent/CS233318B1/cs unknown
- 1981-03-13 DE DE19813109748 patent/DE3109748A1/de not_active Withdrawn

Also Published As

Publication number	Publication date
SU1731813A1 (ru)	1992-05-07
CS233318B1 (en)	1985-02-14
BG41060A1 (en)	1987-04-15
DE3109748A1 (de)	1982-05-06

Legal Events

Date	Code	Title	Description
1996-05-09	ENJ	Ceased due to non-payment of renewal fee

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