DD249037A1 - Verfahren zur synthese von streptokinase durch heterologe produzenten - Google Patents

Verfahren zur synthese von streptokinase durch heterologe produzenten Download PDF

Info

Publication number: DD249037A1
Authority: DD; German Democratic Republic
Prior art keywords: skc; streptokinase; dna; strains; coli
Prior art date: 1983-10-10

Application number

DD25552383A

Other languages

German (de)

English (en)

Inventor

Horst Malke

Joseph J Ferretti

Original Assignee

Adw Ddr

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-10-10

Filing date

1983-10-10

Publication date

1987-08-26

1983-10-10 Application filed by Adw Ddr filed Critical Adw Ddr

1983-10-10 Priority to DD25552383A priority Critical patent/DD249037A1/de

1984-10-05 Priority to AU33859/84A priority patent/AU561372B2/en

1984-10-08 Priority to GR80577A priority patent/GR80577B/el

1984-10-08 Priority to AT84306851T priority patent/ATE61816T1/de

1984-10-08 Priority to DE8484306851T priority patent/DE3484304D1/de

1984-10-08 Priority to ZA847873A priority patent/ZA847873B/xx

1984-10-08 Priority to EP84306851A priority patent/EP0151337B1/en

1984-10-09 Priority to JP21240384A priority patent/JPS60237995A/ja

1984-10-09 Priority to NO844039A priority patent/NO844039L/no

1984-10-09 Priority to DK482284A priority patent/DK482284A/da

1984-10-09 Priority to ES536623A priority patent/ES8702948A1/es

1984-10-09 Priority to CA000464939A priority patent/CA1223223A/en

1984-10-09 Priority to FI843963A priority patent/FI843963L/fi

1987-08-26 Publication of DD249037A1 publication Critical patent/DD249037A1/de

Links

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Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)

DD25552383A 1983-10-10 1983-10-10 Verfahren zur synthese von streptokinase durch heterologe produzenten DD249037A1 (de)

Priority Applications (13)

Application Number	Priority Date	Filing Date	Title
DD25552383A DD249037A1 (de)	1983-10-10	1983-10-10	Verfahren zur synthese von streptokinase durch heterologe produzenten
AU33859/84A AU561372B2 (en)	1983-10-10	1984-10-05	Streptokinase-coding recombinant vectors
GR80577A GR80577B (en)	1983-10-10	1984-10-08	Streptokinase - coding recombinant vectors
AT84306851T ATE61816T1 (de)	1983-10-10	1984-10-08	Streptokinase kodierende rekombinante vektoren.
DE8484306851T DE3484304D1 (de)	1983-10-10	1984-10-08	Streptokinase kodierende rekombinante vektoren.
ZA847873A ZA847873B (en)	1983-10-10	1984-10-08	Streptokinase-coding recombinant vectors
EP84306851A EP0151337B1 (en)	1983-10-10	1984-10-08	Streptokinase-coding recombinant vectors
JP21240384A JPS60237995A (ja)	1983-10-10	1984-10-09	ストレプトキナ−ゼ産生のための組換えベクタ−
NO844039A NO844039L (no)	1983-10-10	1984-10-09	Streptokinase kodende rekombinant vektorer
DK482284A DK482284A (da)	1983-10-10	1984-10-09	Rekombinante vektorer, der koder for streptokinase, og fremgangsmaadetil fremstilling af saadanne vektorer
ES536623A ES8702948A1 (es)	1983-10-10	1984-10-09	Procedimiento para preparar estreptoquinasa
CA000464939A CA1223223A (en)	1983-10-10	1984-10-09	Streptokinase-coding recombinant vectors
FI843963A FI843963L (fi)	1983-10-10	1984-10-09	Streptokinas kodande kombinationsvektorer.

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DD25552383A DD249037A1 (de)	1983-10-10	1983-10-10	Verfahren zur synthese von streptokinase durch heterologe produzenten

Publications (1)

Publication Number	Publication Date
DD249037A1 true DD249037A1 (de)	1987-08-26

Family

ID=5550980

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD25552383A DD249037A1 (de)	1983-10-10	1983-10-10	Verfahren zur synthese von streptokinase durch heterologe produzenten

Country Status (4)

Country	Link
JP (1)	JPS60237995A (el)
DD (1)	DD249037A1 (el)
GR (1)	GR80577B (el)
ZA (1)	ZA847873B (el)

1983
- 1983-10-10 DD DD25552383A patent/DD249037A1/de not_active IP Right Cessation
1984
- 1984-10-08 ZA ZA847873A patent/ZA847873B/xx unknown
- 1984-10-08 GR GR80577A patent/GR80577B/el unknown
- 1984-10-09 JP JP21240384A patent/JPS60237995A/ja active Pending

Also Published As

Publication number	Publication date
GR80577B (en)	1985-02-12
ZA847873B (en)	1985-05-29
JPS60237995A (ja)	1985-11-26

Legal Events

Date	Code	Title	Description
1994-01-27	UW	Conversion of economic patent into exclusive patent
1995-08-31	ENJ	Ceased due to non-payment of renewal fee

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JPH10500012A (ja)	1998-01-06	溶解素による乳酸バクテリアの培養物を溶解する方法および得られる溶解培養物の使用
DE60023158T2 (de)	2006-09-28	Phagenresistentes streptococcus thermophilus
Malouin et al.	1987	Cloning and expression of genes responsible for altered penicillin-binding proteins 3a and 3b in Haemophilus influenzae
DE69126389T2 (de)	1997-12-18	Phagenabwehr Rotationsstrategie
DE3787565T2 (de)	1994-04-14	Plasmiden, die bei Bakterien Phageunempfindlichkeit verleihen.
EP0187138B1 (de)	1992-04-15	Mikroorganismus und Plasmid für konstitutive Creatinamidino-hydrolase-Bildung sowie Verfahren zur Herstellung derselben
EP0524604B1 (de)	1998-10-14	Gentechnologisches Verfahren zur Herstellung von S-(+)-2,2-Dimethylcyclopropancarboxamid mittels Mikroorganismen
DD273284A5 (de)	1989-11-08	Verfahren zur Synthese von Streptokinase durch heterologe produzenten
DE3689993T2 (de)	1994-11-24	Vektor für Expression und Sekretion von Produkten von heterologen Genen in Bacillus Subtilis.
EP0150424B1 (de)	1991-08-28	Unikale, monogenetische Streptokinase, ihre Herstellung und Verwendung