DE3852004D1 - Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. - Google Patents
Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.Info
- Publication number
- DE3852004D1 DE3852004D1 DE3852004T DE3852004T DE3852004D1 DE 3852004 D1 DE3852004 D1 DE 3852004D1 DE 3852004 T DE3852004 T DE 3852004T DE 3852004 T DE3852004 T DE 3852004T DE 3852004 D1 DE3852004 D1 DE 3852004D1
- Authority
- DE
- Germany
- Prior art keywords
- genes
- cells
- expression
- microcultures
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 230000001164 bioregulatory effect Effects 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 5
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 235000011056 potassium acetate Nutrition 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 229920002477 rna polymer Polymers 0.000 abstract 1
- 238000011896 sensitive detection Methods 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000001632 sodium acetate Substances 0.000 abstract 1
- 235000017281 sodium acetate Nutrition 0.000 abstract 1
- 230000010474 transient expression Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPI145587 | 1987-04-15 | ||
| PCT/AU1988/000099 WO1988008038A1 (en) | 1987-04-15 | 1988-04-05 | Method for detecting the expression of bioregulatory genes in microcultures of eukaryotic cells and kit therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3852004D1 true DE3852004D1 (de) | 1994-12-08 |
| DE3852004T2 DE3852004T2 (de) | 1995-06-01 |
Family
ID=3772116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE3852004T Expired - Fee Related DE3852004T2 (de) | 1987-04-15 | 1988-04-05 | Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0309558B1 (de) |
| JP (1) | JPH01503598A (de) |
| AT (1) | ATE113665T1 (de) |
| DE (1) | DE3852004T2 (de) |
| ES (1) | ES2009261A6 (de) |
| PT (1) | PT87224B (de) |
| WO (1) | WO1988008038A1 (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0534640B1 (de) * | 1991-09-23 | 1996-10-02 | Pfizer Inc. | Verfahren für die Detektion von spezifische mRNS und DNS in Zellen |
| US5837467A (en) * | 1997-01-21 | 1998-11-17 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Method for predicting the response to treatment with BCG of superficial bladder carcinoma |
| US20010046665A1 (en) * | 2000-01-21 | 2001-11-29 | Powell Thomas J. | Method of identifying the function of a test agent |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4483920A (en) * | 1982-05-17 | 1984-11-20 | Hahnemann University | Immobilization of message RNA directly from cells onto filter material |
| ES8600404A1 (es) * | 1983-05-02 | 1985-10-01 | Enzo Biochem Inc | Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico. |
| FR2552879B1 (fr) * | 1983-10-03 | 1987-08-14 | Pasteur Institut | Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes |
| CA1301606C (en) * | 1986-05-02 | 1992-05-26 | David H. Gillespie | Chaotropic method for evaluating nucleic acids in a biological sample |
-
1988
- 1988-04-05 JP JP63503796A patent/JPH01503598A/ja active Pending
- 1988-04-05 DE DE3852004T patent/DE3852004T2/de not_active Expired - Fee Related
- 1988-04-05 EP EP88903742A patent/EP0309558B1/de not_active Expired - Lifetime
- 1988-04-05 WO PCT/AU1988/000099 patent/WO1988008038A1/en not_active Ceased
- 1988-04-05 AT AT88903742T patent/ATE113665T1/de not_active IP Right Cessation
- 1988-04-12 PT PT87224A patent/PT87224B/pt not_active IP Right Cessation
- 1988-04-13 ES ES8801135A patent/ES2009261A6/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0309558A4 (en) | 1992-04-01 |
| WO1988008038A1 (en) | 1988-10-20 |
| EP0309558A1 (de) | 1989-04-05 |
| ES2009261A6 (es) | 1989-09-16 |
| ATE113665T1 (de) | 1994-11-15 |
| JPH01503598A (ja) | 1989-12-07 |
| PT87224B (pt) | 1992-08-31 |
| EP0309558B1 (de) | 1994-11-02 |
| PT87224A (pt) | 1988-05-01 |
| DE3852004T2 (de) | 1995-06-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |