ES2009261A6 - Procedimiento para detectar la expresion de genes biorreguladores en microcultivos de celulas eucarioticas. - Google Patents

Procedimiento para detectar la expresion de genes biorreguladores en microcultivos de celulas eucarioticas.

Info

Publication number
ES2009261A6
ES2009261A6 ES8801135A ES8801135A ES2009261A6 ES 2009261 A6 ES2009261 A6 ES 2009261A6 ES 8801135 A ES8801135 A ES 8801135A ES 8801135 A ES8801135 A ES 8801135A ES 2009261 A6 ES2009261 A6 ES 2009261A6
Authority
ES
Spain
Prior art keywords
genes
cells
expression
microcultures
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8801135A
Other languages
English (en)
Inventor
Raymond Kaempfer
Gila Arrad
Lisya Gerez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of ES2009261A6 publication Critical patent/ES2009261A6/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA DETECTAR LA EXPRESION DE GENES BIORREGULARES EN MICROCULTIVOS DE CELULAS EUCARIOTICAS, COMPRENDE: (A) AISLAR Y CULTIVAR DICHAS CELULAS EN CONDICIONES QUE INDUZCAN LA EXPRESION DE POR LO MENOS UNO DE DICHOS GENES; (B) LISAR DICHAS CELULAS CULTIVADAS CON HIDROCLORURO DE GUANIDINIO; (C) PRECIPITAR SELECTIVAMENTE ACIDO RIBONUCLEICO POR ADICION, AL PREPARADO CELULAR HOMOGENEIZADO, DE UNA SOLUCION DE ACETATO SODICO O POTASICO, SEGUIDO POR ETANOL ABSOLUTO; (D) RECUPERAR Y TRANSFERIR RNA SOBRE UNA MATRIZ SOPORTE; (E) HIBRIDIZAR EL RNA RECUPERADO Y TRANSFERIDO CON UNA RIBOSONDA MARCADA ESPECIFICA A POR LO MENOS UNO DE DICHO GENES; Y (F) DETERMINAR CUANTITATIVAMENTE EL GRADO DE LIGACION DE DICHA RIBOSONDA MARCADA A DICHO RNA TRANSFERIDO.
ES8801135A 1987-04-15 1988-04-13 Procedimiento para detectar la expresion de genes biorreguladores en microcultivos de celulas eucarioticas. Expired ES2009261A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPI145587 1987-04-15

Publications (1)

Publication Number Publication Date
ES2009261A6 true ES2009261A6 (es) 1989-09-16

Family

ID=3772116

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8801135A Expired ES2009261A6 (es) 1987-04-15 1988-04-13 Procedimiento para detectar la expresion de genes biorreguladores en microcultivos de celulas eucarioticas.

Country Status (7)

Country Link
EP (1) EP0309558B1 (es)
JP (1) JPH01503598A (es)
AT (1) ATE113665T1 (es)
DE (1) DE3852004T2 (es)
ES (1) ES2009261A6 (es)
PT (1) PT87224B (es)
WO (1) WO1988008038A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE143700T1 (de) * 1991-09-23 1996-10-15 Pfizer Verfahren für die detektion von spezifische mrns und dns in zellen
US5837467A (en) * 1997-01-21 1998-11-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Method for predicting the response to treatment with BCG of superficial bladder carcinoma
US20010046665A1 (en) * 2000-01-21 2001-11-29 Powell Thomas J. Method of identifying the function of a test agent

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
ES8600404A1 (es) * 1983-05-02 1985-10-01 Enzo Biochem Inc Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico.
FR2552879B1 (fr) * 1983-10-03 1987-08-14 Pasteur Institut Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample

Also Published As

Publication number Publication date
WO1988008038A1 (en) 1988-10-20
EP0309558A4 (en) 1992-04-01
EP0309558A1 (en) 1989-04-05
DE3852004T2 (de) 1995-06-01
EP0309558B1 (en) 1994-11-02
PT87224B (pt) 1992-08-31
DE3852004D1 (de) 1994-12-08
JPH01503598A (ja) 1989-12-07
ATE113665T1 (de) 1994-11-15
PT87224A (pt) 1988-05-01

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FD1A Patent lapsed

Effective date: 20000301